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Citations for New England Biolabs :
1801 - 1850 of 10000+ citations for Protein Digestion kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... These amplicons were used as templates for in vitro transcription using the HiScribe T7 mRNA Kit with CleanCap® Reagent AG kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription into cDNA was performed using a cDNA synthesis kit (ProtoScript First Strand cDNA Synthesis Kit, New England BioLabs, Ipswich) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were generated using the NEBNext Globin&rRNA depletion kit and the NEBNext UltraII Directional RNA Library prep kit according to the manufacturer’s protocols (New England Biolabs, MA, USA), as described previously58 ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were qualified on Agilent 2100 Bioanalyzer using High Sensitivity DNA Kit and quantified by qPCR using NEBNext Library Quant Kit for Illumina (NEB, E7630). Equal molarity of each library was pooled and subjected to sequencing on Illumina NovaSeq 6000 platform at the McGill University and Génome Québec Innovation Centre to generate 100 bp paired-end reads.
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bioRxiv - Biophysics 2024Quote: ... Vector DNA was linearized with NheI or MluI and mRNA synthesized in vitro using the SP6 or T7 AmpliCap Max High Yield Message Maker Kit (Cellscript, USA) or HiScribe® T7 ARCA mRNA Kit (New England Biolabs).
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bioRxiv - Evolutionary Biology 2024Quote: ... Poly-A selection for mRNA enrichment was performed using the corresponding NEB kit (#E7490) followed by library preparation using the directional RNA library prep kit from NEB (#E7765). An additional NEB kit Low Input RNA library prep kit (#E6420 ...
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bioRxiv - Molecular Biology 2019Quote: ... After annealing the complex an equimolar amount was mixed with 1000ng Cas9 recombinant protein (NEB; final conc 20ng/ul) and incubated at RT for 15’ ...
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bioRxiv - Cell Biology 2020Quote: ... 40 µl of the cleared lysates were incubated with 1 µl Lambda Protein Phosphatase (λ phosphatase) (New England Biolabs), 5 µl 10x buffer for Protein MetalloPhosphatases (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... The reactions were stopped by adding EDTA and the proteins were digested by adding Proteinase K (New England Biolabs) at 37℃ for 20min ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mg of cell lysate was cleared with protein A magnetic beads to remove non-specific interactions (S1425S, NEB) and incubated overnight with anti-ZAP antibody (Proteintech 16820-1-AP ...
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bioRxiv - Genetics 2019Quote: P2C-Cas9 and P2C-EGFP proteins were expressed from pET28a-P2C-Cas9 and pRSET-P2C-EGFP respectively by recombinant BL21 E.coli (NEB) as described in detail in Chaverra-Rodriguez et al ...
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bioRxiv - Cell Biology 2019Quote: ... the purified C-I30-Flag protein (30µl beads) was digested on beads (Anti-FLAG M2 affinity gel) with final 5U Enterokinase (cat#: P8070S, NEB) in the 1x EK reaction buffer (20 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2020Quote: ... and 1.5 mg of protein was cleaved in a 2 ml reaction with 240 Units of TEV protease (NEB) for two hours at 30 °C ...
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bioRxiv - Bioengineering 2021Quote: ... and cleavage of the fusion protein with enterokinase that were performed according to manufacturer’s instructions (amylose resin purification – according to pMAL Protein Fusion & Purification System, New England Biolabs, Ltd. ...
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bioRxiv - Biochemistry 2021Quote: ... 500 μg of “heavy” or “light” THP-1 protein extract were dephosphorylated with 50 U of Antarctic phosphatase (NEB) in Antarctic phosphatase buffer (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
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bioRxiv - Cell Biology 2021Quote: ... beads were pelleted and resuspended in 100 μl of phosphatase buffer with lambda protein phosphatase (P0753; New England Biolabs) before agitation at 30°C for 30 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... 20 μg each of the recombinant proteins were lyophilized and digested with PNGase F (P0701S, New England Biolabs Inc.) according to the manufacture’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... centrifuged at 58,000 × g for 50 minutes at 4°C and the protein was batch purified using chitin beads (NEB). Protein-bound chitin beads were washed with lysis buffer and high salt buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2020Quote: All recombinant CPK28 clones were transformed into lambda phosphatase-expressing BL21 (DE3) E.coli cells for the production of dephosphorylated proteins or into T7 Express cells (New England Biolabs) for production of hyperphosphorylated protein (53) ...
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bioRxiv - Microbiology 2021Quote: ... benthamiana using the method described in the immunoblotting section were treated with Protein Deglycosylation Mix II (New England BioLabs) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... As a marker 70 µl of Color Protein Standard Broad Range (New England BioLabs, #P7712S; 25 to 245 kD) diluted 1/7 in SDS sample buffer was loaded ...
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bioRxiv - Cell Biology 2021Quote: ... One sample was left untreated and the other two were supplemented with 1X Protein MetalloPhosphatase buffer (New England Biolabs) and 1 mM MnCl2 only or together with 1 μl Lambda Protein Phosphatase (New England Biolabs) ...
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bioRxiv - Biophysics 2021Quote: ... All proteins were purified and labeled with BG-surface Alexa 647 and BC-surface Dy547 dyes (New England Biolabs) as previously described (Murayama and Uhlmann ...
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bioRxiv - Cell Biology 2020Quote: The remainder of the protein was incubated with 50 μl of Ni-NTA Magnetic Beads (New England Biolabs, Inc.) on a rocker for 45 min at room temperature ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 1:8 dilution Cas9 protein (New England Biolabs, cat. M0386M; diluted in buffer B, New England Biolabs, cat. B802S) and 1:40 dilution of phenol red (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation (20 min, 20,000 × g, 4°C) and protein was purified on amylose resin (NEB) including a high salt wash with buffer containing 25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... Protein samples were boiled in 1X Denaturing Buffer and incubated with PNGase F or Endo Hf (New England Biolabs) for 1-1.5 hr at 37°C following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Clarified lysates were incubated with 0.9 mM MnCl2 and 7 U/μl lysate lambda protein phosphatase (λPP; New England BioLabs), or 0.9 mM MnCl2 and H2O as control ...
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bioRxiv - Neuroscience 2020Quote: ... To perform in vitro phosphorylation 5 μg of purified GST-NL2CT (WT or S714D) fusion protein was incubated with 2.5kUnits purified catalytic subunit of PKA (NEB) supplemented with 200 μM ATP at 30°C for the indicated time points.
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bioRxiv - Biochemistry 2022Quote: ... The N-linked glycans and the CBP tag were cleaved by endoglycosidase H (Endo H, ∼0.2 mg per 10–15 mg purified protein, New England Biolabs) and HRV-3C protease (∼2 mg per 10–15 mg purified proteins ...
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bioRxiv - Biochemistry 2019Quote: ... All sens RNA strands were labeled at their 5’ end with [γ-32P] ATP using protein nucleotide kinase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... 115 amino acids) fused to the C-terminus of maltose binding protein (MBP) in pMALX vector (New England Biolabs), MBP-5-HT3A-ICD-pMALX ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 µg of genomic DNA was incubated with 3 µg of in vitro transcribed sgRNAs and 3 µg of purified Cas9 protein in 20µl of 1x NEB3 buffer (New England Biolabs) at 37°C over night ...
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bioRxiv - Developmental Biology 2019Quote: ... single copy scGESTALT.2 F1 male transgenic adults were crossed to wildtype female adults and one-cell embryos were injected with 1.5 nl of Cas9 protein (NEB) and sgRNAs 1-8 in salt solution (8 µM Cas9 ...
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bioRxiv - Microbiology 2021Quote: ... the nuclei were pelleted and supernatants were transferred to 2.0 ml screw-top tubes with O-rings containing magnetic beads (SureBeads Protein G Magnetic Beads, NEB) coupled to antibodies (anti-pan Ago antibody ...
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bioRxiv - Genetics 2020Quote: ... and resuspended in 750 µl ligation mixture containing 200 units T4 DNA ligase (NEB/ IMB core facility protein production), 1X T4-Ligation buffer (NEB ...
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bioRxiv - Biochemistry 2020Quote: The total volume of CRISPR Cas12a-based cleavage assay was 20 μL including LbCas12a protein (New England BioLabs, Inc.), crRNA ...
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bioRxiv - Cancer Biology 2019Quote: ... 25ug of each protein lysate were diluted into 3xSDS gel loading buffer (New England Biolabs Cat# B7703S, Lpswich, MA) and heated to 95°C for 5 minutes ...
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bioRxiv - Genetics 2020Quote: ... A 6 µL mixture containing 2 µL of 40 µM Spy Cas9 NLS protein (New England Biolabs, MA, USA), 200 ng each of five sgRNAs (in 2 µL ...
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bioRxiv - Biochemistry 2021Quote: ... The purified target PCR amplicon was incubated with purified recombinant wt- or en-Cas12a protein and crRNA (RNA-guides or chimeric DNA-RNA guides) in 10X buffer (NEBuffer3.1, NEB) for 1 h ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... proteins (2 mg mL-1) were incubated with 180000 U mL-1 PNGase F (New England Biolabs, Unites States) in PBS (pH 7.4 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of each sgRNA and 60 pmol Cas9-NLS protein (EnGen® Spy Cas9 NLS, NEB, M0646 M) with the Lonza 4D X-unit ...
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bioRxiv - Genetics 2021Quote: ... Five µl of the hybridization product was combined with 1 µl of 20 µM Cas9 protein (New England Biolabs) at room temperaturefor 5 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Expression of all SurA and SurA-OmpA proteins was carried out using chemically competent home-made stocks of E.coli strain BL21(DE3) originally sourced commercially (NEB). Both proteins were over-produced separately in 1 L of cultures as described previously [42] ...
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bioRxiv - Biochemistry 2022Quote: ... Expression of all soluble proteins used chemically competent home-made stocks of E.coli strain BL21(DE3) originally sourced commercially (NEB).
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bioRxiv - Cell Biology 2022Quote: ... Protein dephosphorylation was achieved by incubation of 20 μg sample with 400 units of λ phosphatase (New England Biolabs) in 10X NEBuffer for protein MetalloPhosphatases (PMP ...
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bioRxiv - Molecular Biology 2022Quote: ... a mixture of 0.5 μg of IMAC purified protein and 0.5 μg BSA (New England Biolabs, Ipswich, MA, USA) in 100 μl of coating buffer per well was added to 384-well microtiter plates (1 μg without BSA for direct detection) ...
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bioRxiv - Biophysics 2022Quote: ... and hCFTR-containing fractions based on SDS-PAGE analysis were pooled after thawing and immediately phosphorylated with 1,000 U of protein kinase A (New England BioLabs) per ml for ∼1 hr on ice prior to addition of VX770 and VX445 to some samples and final purification via Superose 6 Increase 3.2/300 gl microbore gel-filtration chromatography in glycerol-free TMN buffer with 150 µM ATP ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 x 106 PDCL-1108 cells were electroporated with 10 μg spCas9-NLS protein (New England Biolabs, Ipswich, MA), 4 μg guide RNA and 200 pmol ssODN using the Amaxa Nucleofector II (Lonza ...