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Citations for New England Biolabs :
1801 - 1850 of 9563 citations for Mouse Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... mRNAs were purified using the Monarch RNA Cleanup Kit (New England BioLabs) and eluted in RNAse-free water ...
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bioRxiv - Cell Biology 2024Quote: ... and NEBNext Ultra TMII Directional RNA Library Prep Kit (New England Biolabs). Libraries were sequenced with an Illumina NovaSeq 6000 to obtain 150 bp ×2 paired-end reads ...
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bioRxiv - Genetics 2024Quote: All plasmids were constructed using Hifi DNA Assembly Cloning Kit (NEB, USA) and then midipreped with ZymoPure Midiprep Kit (Zymo Research ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... The RNA samples were cleaned with Monarch RNA Cleanup Kit (NEB #T2030) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasmid DNA was extracted using the Monarch Miniprep Kit (NEB, Cat. #T1010L) and sent to sequencing to identify mutations.
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bioRxiv - Developmental Biology 2024Quote: ... using the NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA).
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: Total RNA was extracted with Monarch Total RNA Miniprep Kit (NEB #T2010S). The integrity of the RNA was determined using the TapeStation 4150 (High Sensitivity RNA kit ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were purified using the Monarch PCR Cleanup Kit (NEB, T1030L) and digested with the TfiI restriction enzyme (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs, Ipswich, MA).
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bioRxiv - Developmental Biology 2024Quote: ... using the NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA) and recombined into a UASp-attB destination vector (gift of F ...
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bioRxiv - Microbiology 2024Quote: ... The Primer-free LunaScript® RT Master Mix Kit (New England Biolabs) was used for first strand cDNA synthesis using a gene-specific primer targeting the mCherry gene in the genomic vRNA (primer sequence 5’-CGCTTCAAGGTGCACATGG-3’) ...
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bioRxiv - Cell Biology 2024Quote: ... The insertion was done using the Q5 Site-Directed Mutagenesis Kit (NEB) and design of respective primers using the NEBaseChanger online tool (http://nebasechanger.neb.com/).
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bioRxiv - Cell Biology 2024Quote: ... and plasmids were purified using a Monarch Plasmid Miniprep Kit (NEB #T1010). Successful mutagenesis was verified through DNA sequencing (Plasmidsaurus).
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bioRxiv - Cell Biology 2024Quote: ... and plasmids were purified using a Monarch Plasmid Miniprep Kit (NEB #T1010). Constructs were then verified by restriction digest using SacI-HF (NEB #R3156 ...
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bioRxiv - Cell Biology 2024Quote: ... The RNA was then made using a T7 Highscribe kit (NEB #E2040S), treated with DNase I (NEB #M0303L ...
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bioRxiv - Microbiology 2024Quote: ... using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, E0554S). Homology-directed repair templates were PCR amplified using the CloneAmp™ HiFi PCR Premix (Takara Bio ...
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bioRxiv - Microbiology 2024Quote: ... and HiFi DNA Assembly kit were purchased from New England Biolabs (NEB). All bacterial strains were PCR or sequence verified before use ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA was extracted using the Monarch gDNA Purification Kit (NEB T3010S) following the standard protocol but with elution using 175 µL 25 mM K3BO3 at pH 7.0.
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared with the NEBNext Ultra II Directional RNA Kit (NEB). Samples were equimolarly pooled and sequenced on a NextSeq550 system (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... PCR-amplified DNA fragments were assembled by Gibson assembly kit (NEBuilder, NEB) as per manufacturer’s protocol and introduced into DH5α E ...
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bioRxiv - Cell Biology 2024Quote: ... we use the Monarch® Genomic DNA Purification Kit (New England Biolabs) and follow the manufacturer recommendation ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA samples were additionally processed with the Monarch RNA Cleanup Kit (NEB), analyzed for integrity with a TapeStation (Agilent) ...
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bioRxiv - Cell Biology 2024Quote: Monarch® Genomic DNA Purification Kit (New England Biolabs, cat. no. T3010S)
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bioRxiv - Cell Biology 2024Quote: Monarch® PCR & DNA Cleanup Kit (New England Biolabs, cat. no. T1030S)
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to manufacturer’s directions ...
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bioRxiv - Molecular Biology 2024Quote: ... In-vitro transcription (HiScribe T7 High Yield RNA Synthesis Kit E2040L, NEB) from the DNA was then carried out ...
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bioRxiv - Molecular Biology 2024Quote: ... rRNA depletion was conducted using NEBNext rRNA depletion kit v2 (NEB, #E7400L) followed by end-repair using T4 PNK after purification of the rRNA depleted samples using Oligo Clean & Concentrator Kits (Zymo research ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized using LunaScript RT SuperMix Kit (New England Biolabs, USA). SYBR green assay was used to determine HBV pregenomic expression (Luna Universal qPCR Master Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... Then we used ProtoScript® II First Strand cDNA Synthesis Kit (NEB) and 3′-RACE CDS primer (ClonTech ...
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bioRxiv - Molecular Biology 2024Quote: ... then purified using the Monarch RNA Cleanup Kit (T2040L, New England Biolabs). Quality and size of the produced RNAs were assessed using an Agilent Fragment Analyzer.
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription was performed with the LunaScript RT SuperMix Kit (#E3010, NEB) using the manufacturer’s protocol on a SimpliAmp thermal cycler (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids were recovered using Monarch Plasmid Miniprep Kit (New England Biolabs #T1010S) or E.Z.N.A ...
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bioRxiv - Microbiology 2024Quote: ... Digested products were cleaned using Monarch® PCR & DNA Cleanup Kit (NEB) and incubated with T4 DNA ligase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and cleaned with the Monarch RNA Cleanup Kit (NEB, Ipswich, MA, USA). Ribosomal 23S and 16S samples were prepared by amplifying the appropriate sequences from genomic E ...
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bioRxiv - Microbiology 2024Quote: Total RNA samples were treated with a NEBNext rRNA depletion kit (NEB) to remove rRNA ...
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bioRxiv - Biochemistry 2024Quote: ... Transcription was performed using T7 HiScribe RNA synthesis kit (New England Biolabs). Generated RNA was subjected to DNase treatment (TURBODNase ...
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bioRxiv - Cancer Biology 2024Quote: ... using the NEBNext® Ultra TM II DNA Library Prep Kit (NEB E7645S/L ...
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bioRxiv - Molecular Biology 2024Quote: ... and purified with the Monarch Total RNA Miniprep Kit (New England Biolabs). Alkylated RNA was purified with the Monarch RNA Cleanup Kit (New England Biolabs) ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were purified using the Monarch PCR Cleanup Kit (NEB, T1030L). PCR products were A-tailed ...
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bioRxiv - Systems Biology 2023Quote: ... Signals following a 1h incubation at room temperature with the appropriate HRP-conjugated secondary antibodies (anti-mouse NEB 7076S or anti-rabbit NEB 7074S) were acquired using the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... total RNA was collected and prepared using Monarch Total RNA Miniprep Kits (NEB). For SARS-CoV-2 experiments ...
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bioRxiv - Plant Biology 2020Quote: ... followed by subcloning into pEAQ-HT vector using the Gibson assembly kit (NEB). Linearization of pEAQ-HT vector was performed by XhoI and AgeI restriction enzyme double digestion ...
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bioRxiv - Plant Biology 2019Quote: ... The libraries were prepared using the Next Ultra RNA Library Prep Kit (NEB) by the company Novogene (China) ...
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bioRxiv - Microbiology 2020Quote: ... chloramphenicol or apramycin resistance cassette by using the Gibson isothermal assembly kit (NEB) followed by PCR amplification using the primers from the extremities ...
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bioRxiv - Microbiology 2020Quote: ... followed by vector-insert ligation using the Quick Ligation kit (New England Biolabs) to generate the original and mutated pCAGGS-HHRz-3M-eGFP-5M vectors ...
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bioRxiv - Cell Biology 2019Quote: ... rRNA was depleted using the NEBNext rRNA Depletion Kit (E6310, New England Biolabs) and subsequently directional RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420 ...
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bioRxiv - Immunology 2021Quote: ... The released DNA was cleaned up using Monarch DNA PCR Clean Kit (NEB) and eluted in TE buffer ...
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bioRxiv - Genetics 2021Quote: SpyCas9 tyr and tbxta sgRNAs were synthesized using EnGen sgRNA Synthesis Kit (NEB). SauCas9 ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplicon was purified using the Monarch PCR and DNA Cleanup Kit (NEB), and 1 ug used as template for transcription of digoxigenin labelled riboprobes using the DIG RNA Labelling mix (Sigma).
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bioRxiv - Genetics 2021Quote: ... The NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) was used for library preparation ...