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Citations for New England Biolabs :
1701 - 1750 of 10000+ citations for Urea Nitrogen BUN Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... PCR amplification was performed in a 10 µl reaction volume containing 1X PCR Buffer (New England Biolabs), 10 µM dNTP and 0.05 U Taq DNA polymerase (New England Biolabs) ...
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bioRxiv - Genomics 2022Quote: ... The mixture electroporated into 100 μL of 10-beta E.coli (NEB, C3020K, 2kV, 200 ohm, 25 μF) in order to achieve a transformation efficiency equal to 300-times the number of unique oligos sequences (target CFU ...
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bioRxiv - Genomics 2020Quote: ... DNA libraries were amplified by PCR for 6-10 cycles with Phusion HF DNA Polymearse (NEB, M0530) using Illumina TruSeq indexing primers for multiplexing ...
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bioRxiv - Molecular Biology 2021Quote: ... the resin was resuspended in 100 μL of NLS Buffer and 10 μL of Proteinase K (NEB) and the sample was incubated at 50°C for 30 minutes while shaking at 1200 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... Images were collected every 10 mins for up to 6 days and the timing of events (NEB, the start of cytokinetic furrow ingression and the appearance of 2 distinct cells ...
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bioRxiv - Genetics 2020Quote: ... Sense oligos diluted to 10 μM in nuclease-free water were phosphorylated with T4 PNK (NEB #M0201) supplemented with ATP ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... 10–20% were sequentially digested with a mix of restriction enzymes according to the manufacturer’s instructions (NEB) 69 ...
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bioRxiv - Molecular Biology 2019Quote: ... Subsequently 5’ and 3’ termini were ligated using 10 units of T4 RNA ligase (New England Biolabs) at 37°C for 1 h ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Commercial competent cells were used for the K-12 DH10B strain (NEB® 10-beta high efficiency). Transformation of B REL606 and B REL607 was achieved via the chemical competence method ...
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bioRxiv - Bioengineering 2020Quote: GFP1-10 was inserted into the pBAD plasmid via NEBuilder® HiFi DNA Assembly (New England Biolabs): 25 ng of the digested vector ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was incubated with gentle shaking for 1 h with 10 mL of amylose resin (NEB) pre-equilibrated in 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... The optimal 25-μL reaction included 2.5 μL 10× ThermoPol buffer (New England Biolabs, Ipswich, Massachusetts, USA), 2.25 μL each of FIP and BIP (1.8 μM) ...
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bioRxiv - Microbiology 2021Quote: ... 10 µg of total RNAs were subjected to RNA purification using DNase I (New England Biolabs Inc.) and the quality was checked on 2% agarose gel and verified using the Agilent Bioanalyser 2100 (Agilent technologies) ...
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bioRxiv - Biophysics 2022Quote: ... The pooled PCR products were incubated at a 10:1 ratio with DpnI enzyme (New England Biolabs) at room temperature for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Five μg of dsRNAs were incubated with 10 units of ShortCut® RNase III (New England Biolabs) in the buffer conditions recommended by the manufacturer for 20 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Parallel T7 reactions performed at the same time used 1.25 μg XbaI linearised pT7HCV(5’+)341N79wt and pT7HCV(5’+)341N79ko as templates in a 25μl final reaction volume containing 2.5μl 10 x T7 transcription buffer (NEB), 0.6μl RNaseOUT ...
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bioRxiv - Cell Biology 2022Quote: Lysates (20 μg) and media immunoprecipitates were diluted to 10 μL in 2X glycoprotein denaturing buffer (NEB), and boiled for 10 min ...
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bioRxiv - Genomics 2020Quote: ... 10 μg of pUC19 was CpG methylated in vitro using 40 units of M.SssI (NEB, Ipswich, MA) in a 200 μl final reaction volume for 2 h at 37 °C ...
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bioRxiv - Microbiology 2019Quote: ... 10 µg of RNA was incubated with 3 units of DNase I (New England Biolabs, MA, USA) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... A 10-fold dilution of Nt.BstNBI was prepared by dissolving the stock enzyme in Diluent A (NEB) supplemented with 0.1 % Triton X-100 ...
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Structure and mechanism of a Type III CRISPR defence DNA nuclease activated by cyclic oligoadenylatebioRxiv - Biochemistry 2019Quote: ... 10 μl eluted product was incubated with T4 DNA Ligase (New England BioLabs, Ipswich, MA, United States) in ligase buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2019Quote: 2–3 μL of recovered DNA was electroporated into NEB® 10-beta Electrocompetent cells (C320K, NEB) and plated on chloramphenicol LB plates ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was dripped twice through a 10 ml packed volume of amylose resin (New England Biolabs), washed with 5 column volumes of column buffer (20 mM Tris ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL of 5 μM annealed adaptors and 10 μL of Blunt/TA ligase master mix (NEB) was added to each reaction ...
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bioRxiv - Genomics 2021Quote: ... 10 ng A-tailed gel-isolated PCR product and 1 µl T4 DNA ligase (New England Biolabs) were mixed in a total reaction volume of 10 µl and incubated for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10 μM NEBNext Index Primer (NEB E7335, NEB E7500, NEB E7710, NEB E7730, NEB E6609), 1 μL 10 μM NEBNext Universal PCR Primer in 20 μL total volume ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10 μM NEBNext Index Primer (NEB E7335, NEB E7500, NEB E7710, NEB E7730, NEB E6609), 1 μL 10 μM NEBNext Universal PCR Primer in 20 μL total volume ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10 μM NEBNext Index Primer (NEB E7335, NEB E7500, NEB E7710, NEB E7730, NEB E6609), 1 μL 10 μM NEBNext Universal PCR Primer in 20 μL total volume ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL 10 μM NEBNext Index Primer (NEB E7335, NEB E7500, NEB E7710, NEB E7730, NEB E6609), 1 μL 10 μM NEBNext Universal PCR Primer in 20 μL total volume ...
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bioRxiv - Biochemistry 2020Quote: ... 10 µCi/µl) by in vitro (run off) transcription driven by T7 RNA polymerase (New England Biolabs), Next ...
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bioRxiv - Cell Biology 2022Quote: ... Sticky ends were blunted using 10 U of DNA Polymerase I Large (Klenow) Fragment (New England Biolabs). pWPI Puro was digested by PmeI and dephosphorylated using 20 U of calf alkaline phosphatase (New England Biolabs ...
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bioRxiv - Developmental Biology 2020Quote: ... Free adaptor was then removed by addition of 1 μL 10 U/μL 5’-Deadenylase (NEB, M0331S). 1 μL 10 U/μL RecJ Exonuclease (Lucigen/Epicentre ...
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bioRxiv - Microbiology 2021Quote: ... and 10 μl of 2x Quick T4 ligase buffer and 1 μl Quick T4 DNA ligase (NEB) were added and incubated at room temperature overnight ...
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bioRxiv - Genetics 2019Quote: ... eluted with 50 ul of 1x NEB Buffer 3 and treated with 10 units of CIP (NEB) for 1 hour at 37°C ...
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bioRxiv - Genomics 2020Quote: ... 10 million crosslinked cells were lysed and the chromatin digested using 375 U of MboI (NEB, R0147M) for 4 hours at 37°C ...
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bioRxiv - Genetics 2020Quote: ... IP and input samples were incubated with 10 μL of 800 U/mL Proteinase K (NEB P8107S) at 37° for 2 hours ...
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bioRxiv - Bioengineering 2022Quote: ... All ligation reactions were performed in a 10 µL mix with T4 DNA Ligase (New England Biolabs) at 16°C overnight according to the manufactureŕs instructions.
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bioRxiv - Biochemistry 2022Quote: ... 50 pmol of in vitro transcribed or synthetic RNA was incubated with 10 units T4 PNK (NEB) and 2 μl γ-32P-ATP in 1X PNK buffer and incubated for 1 h at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... 10 min) and resuspended in 50 µl of 50 mM Tris-HCl containing 1 µg trypsin (NEB). After overnight incubation at 37 °C ...
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bioRxiv - Biophysics 2024Quote: ... 10 µl of each sample was mixed with 4 µL native loading dye (1% Orange G (NEB) dissolved in 50% glycerol and 50% EMSA buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... nucleic acids were digested by addition of 10 µL of DNaseI (2000 units/mL, New England Biolabs) and CaCl2 to a final concentration of 10 mM ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting plasmid products were transformed into NEB 10-beta electrocompetent cells (New England Biolabs, Ipswich, MA) and purified using a ZymoPURE Midiprep Kit (Zymo Research ...
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bioRxiv - Biochemistry 2024Quote: ... Nicking mutagenesis plasmid products were transformed into NEB 10-beta electrocompetent cells (New England Biolabs, Ipswich, MA) and purified using a ZymoPURE Midiprep Kit (Zymo Research ...
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bioRxiv - Genomics 2023Quote: ... at 10 pmol each and then amplified in 9 cycles using Phusion DNA polymerase (New England Biolabs) from primers that added flanking overlaps to tiles allowing Infusion cloning in a screening vector ...
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bioRxiv - Microbiology 2024Quote: ... followed by DNaseI treatment of the RNA for 10 min at 37°C (New England Biolabs, M0303), and removal of the DNaseI using the RNA clean up kit (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... followed by DNaseI treatment of the RNA for 10 min at 37°C (New England Biolabs, M0303), and removal of the DNaseI using the RNA clean up kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 ul 10 mM dGTP, 1.5 ul 10 mM dTTP, 37.5 ul 0.4 mM biotin-14-dATP, 40 ul NEB buffer2 ...
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bioRxiv - Bioengineering 2023Quote: ... and 1.25 μl of 10 μM each primer using Phusion high-fidelity DNA polymerase (New England BioLabs). After PCR ...
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bioRxiv - Genetics 2024Quote: ... 10 μg of the purified RNA was then polyadenylated by 4U poly(A) polymerase (New England Biolabs) at 37°C for 1 hr and purified again by illustra microspin G-50 column or Direct-zol RNA Miniprep kits (Zymo Research ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA 3′-ends were dephosphorylated in a 10 µL reaction with 2 µL T4 PNK (NEB #M0201S) 1 µL of FastAP Thermosensitive Alkaline Phosphatase (ThermoFisher #EF0651) ...