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Citations for New England Biolabs :
1701 - 1750 of 10000+ citations for Human Carboxyl Terminal PDZ Ligand Of Neuronal Nitric Oxide Synthase Protein NOS1AP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... using the Monarch DNA gel extraction kit (NEB #T020S) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Amplicons were purified with the Monarch PCR kit (NEB), digested with MfeI-HF and SphI-HF (NEB ...
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bioRxiv - Microbiology 2023Quote: ... and NEB Ultra II RNA library prep kit (NEB) were used to prepare complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2023Quote: ... Cloning was done using a Gibson Assembly kit (NEB) and NEB5a competent cells (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... products were purified using an RNA purification kit (NEB), absorption at 260 nm was determined and length of the RNA product was analyzed on a 1 % agarose gel.
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bioRxiv - Molecular Biology 2023Quote: ... then cloned into pCW57.1 using HiFi assembly kit (NEB). All the plasmids were sequenced and verified ...
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bioRxiv - Molecular Biology 2023Quote: ... after rRNA depletion (NEBNext rRNA depletion kit by NEB) using 1 μg of total RNA ...
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bioRxiv - Developmental Biology 2023Quote: ... a Q5® Site-Directed Mutagenesis Kit (NEB #E0552S) was used ...
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bioRxiv - Molecular Biology 2023Quote: The Q5 High-Fidelity Polymerase kit (Cat# M0493, NEB) was used for amplification (5 μL 5× Q5 reaction buffer ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were constructed using Ultra II DNA kit (NEB).
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bioRxiv - Biochemistry 2024Quote: ... and purified with Monarch PCR & DNA cleanup kit (NEB). PURExpress in vitro translation reaction was assembled in RNase-free tubes according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... and Monarch PCR&DNA Cleanup Kit (5 μg) (NEB). The purified dsDNA templates were transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... the Monarch Genomic DNA Purification kit (New England Biolabs) was utilized with 1-2 x 106 cells ...
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A humoral immune response to parasitoid wasps in Drosophila is regulated by JAK/STAT, NF-κB and GATAbioRxiv - Immunology 2024Quote: ... we used Q5 site-directed mutagenesis kit (NEB# E0554S). Primers were designed according to the sites to be scrambled as shown in the table below ...
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bioRxiv - Microbiology 2024Quote: ... spin purified with DNA clean and concentrate kit (NEB), and sequenced with Plasmidsaurus.
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bioRxiv - Microbiology 2024Quote: ... the NEBNext Ultra II DNA Library Prep Kit (NEB) was used according to the manufactu er’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... using Qubit DNA HS kit (New England Biolabs, M0494S). The final RP libraries were single-end sequenced with a NextSeq2000 P2 system (Illumina) ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Quick Ligase kit (M2200, NEB, Ipswich, US). For a site-directed mutagenesis the central guanine of the seed sequence was exchanged for an adenosine ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by ligation (T4 DNA Ligase Kit, M0202, NEB) of the resulting products at a 1:3 molar ratio of vector to insert ...
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bioRxiv - Cell Biology 2024Quote: ... or using NEB LunaScript RT SuperMix kit (NEB #E3010L). In both cases ...
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bioRxiv - Molecular Biology 2024Quote: ... and purified using Monarch RNA Cleanup Kit (NEB, T2030S). 100 ng mRNA was aliquoted and stored as input at –80°C ...
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bioRxiv - Biochemistry 2024Quote: A Q5 Site-directed Mutagenesis Kit (New England Biolabs) was used per the manufacturer’s instructions with the primers in Table S1 and a pET15b-holC plasmid to mutate arginine 128 to alanine in the HolC protein ...
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bioRxiv - Bioengineering 2024Quote: ... and Ultra II Directional RNA Library Prep Kit (NEB) to isolate mRNA and perform dUTP directional preparation of the mRNA library ...
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bioRxiv - Bioengineering 2024Quote: ... Ligations were done with the Quick LigationTM Kit (NEB). NEB® Stable Competent E ...
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bioRxiv - Bioengineering 2024Quote: ... purified using the Monarch DNA Gel Extraction Kit (NEB), confirmed via Sanger sequencing (Genewiz) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and subsequently purified (Monarch® RNA Cleanup Kit, NEB). All tailed and untailed mRNA samples were normalized to a concentration of 50ng/µl and quality was assessed via TapeStation RNA ScreenTape Analysis (Agilent ...
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bioRxiv - Synthetic Biology 2024Quote: ... The Monarch® RNA Cleanup Kit (New England Biolabs) was used to purify transcribed RNA strands ...
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bioRxiv - Molecular Biology 2024Quote: The Q5 Site-Directed Mutagenesis Kit (New England Biolabs) was used to introduce the mutations into pET15b plasmid-borne copies of PRX1 and TRX3 genes using pairs of adequate ...
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bioRxiv - Microbiology 2024Quote: ... purified using the Monarch DNA Gel Extraction kit (NEB) and ligated using T4 DNA ligase (Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... HiFi DNA assembly cloning kit (New England Biolabs (NEB)) was used according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... or HiScribe T7 ARCA mRNA Kit (New England Biolabs), evaluated spectrophotometrically and by gel electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... or Q5 Site-Directed Mutagenesis Kit (New England Biolabs). To improve PCR yield with the GCrich hα1B template ...
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bioRxiv - Microbiology 2024Quote: ... HiFi DNA assembly cloning kit (New England Biolabs (NEB)) was used according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... a Quickchange site-directed mutagenesis kit (New England Biolabs) was used to introduce the F405L and K409R mutations in the HC plasmids of the IgGs to make the bsAbs ...
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bioRxiv - Molecular Biology 2020Quote: ... Lipidation-deficient LC3B proteins were generated by mutagenic PCR using Q5 Hot Start High-Fidelity Master Mix (New England Biolabs) to introduce the GGG to GCT change that leads to a glycine to alanine (G120A ...
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bioRxiv - Immunology 2020Quote: ... was inserted into a published oligonucleotide scaffold (Talbot and Amacher, 2014) and injected together with recombinant Cas9 protein (New England Biolabs) into 1-2 cell stage zebrafish (AB strain) ...
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bioRxiv - Developmental Biology 2020Quote: ... We first joined sequences for fluorescent protein mCerulean (CFP) and 2A peptide P2A (a ribosomal skip sequence) with Q5 (NEB) fusion PCR and added them to the pWPXL vector with Gibson Assembly Master Mix (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... glabrata total cell lysates using the anti-Rad53 antibodies described in the previous section and Protein A magnetic beads (New England Biolabs). The immunoprecipitated samples were run on 8% acrylamide gels ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 mM EDTA) at 65°C for 15 minutes and later the proteins were digested by 10U Proteinase K (NEB) at 37°C for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 μg/well of purified BSA (NEB) in a volume of 50 μl ...
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bioRxiv - Neuroscience 2019Quote: ... pRSET-dNOS exon 16 DNA was then transformed into T7 Express lysY/lq E.coli protein expression cells (New England Biolabs #C3013) and plated on LB plus ampicillin (60 µl/ml) ...
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bioRxiv - Biochemistry 2019Quote: ... Precipitated proteins were removed by centrifugation at 14000g for 30 min at 4°C and the remaining soluble protein was incubated with enterokinase light chain (NEB) for 16h at room-temperature as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... MBP-fusion proteins were purified with amylose resin following the manufacturer’s instructions (GE Healthcare, Pierce, Agilent Technologies and New England Biolabs). MBP-fusions (1 µg each in 100 µl of 1% NP40 ...
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bioRxiv - Cancer Biology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 µg/well of purified BSA (NEB) in a volume of 50 µl ...
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bioRxiv - Neuroscience 2020Quote: ... human parkin were expressed as 6His-Smt3 fusion proteins in Escherichia coli BL21 (DE3) Codon-Plus RIL competent cells (C2527, New England Biolabs) as previous described22,33,52 ...
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bioRxiv - Neuroscience 2021Quote: ... genomic PCR products containing the target sites of selected gRNAs were incubated with SpCas9 protein (New England Biolabs, Ipswich, MA) following the manufacturer’s protocol and analyzed on 2% agarose gel stained with ethidium bromide ...
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bioRxiv - Microbiology 2021Quote: ... The D614G S-protein was generated by introducing the mutation into the Wuhan reference strain via Q5 Site-directed mutagenesis (NEB). Other individual mutations were subsequently introduced into the D614G S by the same process ...
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bioRxiv - Neuroscience 2020Quote: ... human parkin were expressed as 6His-Smt3 fusion proteins in Escherichia coli BL21 (DE3) Codon-Plus RIL competent cells (C2527, New England Biolabs) as previous described 44-46 ...
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bioRxiv - Biochemistry 2020Quote: ... λex = 488 nm and λem = 526 nm were used for detection of covalently bound flavin and λex = 670 nm and λem = 633 nm for visualisation of the Blue Prestained Protein Standard (New England Biolabs).
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bioRxiv - Microbiology 2021Quote: ... or beads alone were suspended in 1x NEBuffer™ for Protein Kinases with 200μM ATP (Thermo) and 100 units of CK2 (NEB) at 30°C for 45 minutes ...