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Citations for New England Biolabs :
1701 - 1750 of 7694 citations for 2R 5S 5 4 amino 5 fluoro 2 oxopyrimidin 1 2H yl 1 3 oxathiolan 2 yl methyl butyrate WXC04778 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: Chemically competent NEB® 5-alpha F’Iq cells (NEB; C2992H) were transformed with recombinant plasmids per manufacturer instructions and grown at 37°C on selective Luria-Bertani (LB ...
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bioRxiv - Bioengineering 2024Quote: ... 5’-end labeling was performed using T4 Polynucleotide Kinase (NEB) and [gamma-32P] ATP (PerkinElmer) ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of T4 DNA ligase (NEB, 400000 U/mL), and 10 μL of 10X T4 DNA ligase buffer (NEB) ...
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bioRxiv - Bioengineering 2024Quote: ... then 5 μL of 6X Purple Loading Dye (NEB, B7024S) were added and run on a GelRed-stained 1% (w/v ...
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bioRxiv - Genomics 2022Quote: ... 2 μl of 10X CutSmart® buffer (1X final) and 2 μl of 1U/μl USER® II enzyme (M5505S, NEB) (0.1U/μl final ...
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bioRxiv - Microbiology 2020Quote: ... stained with a mAb cocktail composed of SARS-CoV-2 spike (Creative-Bios; 2BCE5) and SARS-CoV-2 nucleoprotein (Creative-Biolabs; NP1C7C7) followed by anti-Mouse IgG-HRP (Abcam ab6823 ...
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bioRxiv - Genomics 2021Quote: ... and supernatant was transferred to a new tube and added to 2 μL 10% SDS and 2 μL 20 mg/mL proteinase K (New England Biolabs P8107). Samples were incubated overnight at 65°C to reverse crosslinks ...
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bioRxiv - Genetics 2023Quote: ... 5 µl of 100 ng/µLcDNA diluted from the previous step was combined with 2 µL of block mix and 2 µL of nuclease free water (NEB, AM9937), and then the cDNA block oligo mix was incubated on a thermocycler under the following conditions to allow block oligo mix to bind to the 5′ end and the 3′ end of the cDNA molecule ...
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bioRxiv - Microbiology 2023Quote: ... antibiotic resistance cassette and 2 flanking regions were combined in equimolar amounts and mixed with 2 x HiFi reagent (NEB, UK) and incubated at 50°C for 1 hour ...
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bioRxiv - Genomics 2020Quote: ... 4 μL of 3 U/μL NEB T4 DNA Polymerase (New England Biolabs), 1 μL of 5U/μL Klenow (New England Biolabs) ...
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bioRxiv - Systems Biology 2022Quote: ... 5’-AAAC(N)19-20-3’) by combining 1 μl of each 100 μM oligonucleotide with 1 μl of 10× T4 ligation buffer (NEB cat. no. B0202S), 6.5 μl of water ...
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bioRxiv - Microbiology 2022Quote: ... We then used 1:3 insert to vector ratio in a 1 hour Hifi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). After the HiFi assembly ...
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bioRxiv - Biophysics 2023Quote: ... linker (listed in Supplemental Table S6) was ligated to the 3′ end using T4 RNA Ligase 2 truncated KQ (New England Biolabs #M0373, 400 units per sample) in T4 Ligase reaction buffer (New England Biolabs #B0216 ...
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bioRxiv - Genomics 2021Quote: ... 1 μl of RNAse inhibitor and 3 μl of Antarctic phosphatase (New England BioLabs Inc.). We then performed a kinase treatment adding 4 μl of T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Genomics 2021Quote: ... The 3′ adapter (sequence: AGATCG-GAAGAGCACACGTCTGAACTC) was ligated using T4 RNA Ligase 1 (NEB, M0204L) and purified nascent RNA using streptavidin beads (NEB ...
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bioRxiv - Systems Biology 2024Quote: ... and NEBNext Multiplex Oligos for Illumina Index Primers Set 1 and 3 (NEB E7335S, E7710S) and PCR-amplified for 15 cycles ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Library preparation for sRNA from sperm samples was done using the New England BioLabs kit NEBNext® Multiplex Small RNA Library Prep Set for Illumina® Set 1 and 2 (NEB #E7300 and NEB #E7580). The provided guidelines of NEB were followed for small sample preparation until step 15 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 2 uL of T4 RNA ligase I (NEB M0204S) totaling 20 uL and incubated for 1 hour at 30 C.
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl 10X RtcB reaction buffer (New England Biolabs), 2 μl 1mM GTP ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by adding 2 μl of RNase A (NEB) and incubating at 37 °C for 45 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 1x T4 RNA Ligase 2 reaction buffer (NEB, M0239S), and 0.5 units/µl T4 RNA Ligase 2 (NEB ...
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bioRxiv - Genomics 2020Quote: ... the NEBNext High-Fidelity 2× PCR Master Mix (NEB) was used according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 25 μL NEBNext HiFi 2× PCR Master mix (NEB) was added ...
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bioRxiv - Bioengineering 2021Quote: ... 2 unit of murine RNase inhibitor (New England Biolabs), varying amounts of target RNA ...
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bioRxiv - Bioengineering 2021Quote: ... Its constituents were 1X RNA Ligase 2 buffer (NEB) supplemented with 5% PEG 8000 ...
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bioRxiv - Biochemistry 2020Quote: ... followed by incubation with 2 Units USER enzyme (NEB) for 10 min at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... packed with 2 ml amylose resin (New England Biolabs) equilibrated with buffer AF ...
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bioRxiv - Genomics 2022Quote: ... 0.7 µl T4 ligase (2 M U/ml, NEB), 250 ng DNA (adapters in File S1 ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 units of phi29 DNA polymerase (New England Biolabs), three not to quantified deoxynucleoside triphosphate mix (20 μM) ...
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bioRxiv - Microbiology 2022Quote: ... 20 μl Phusion DNA polymerase (2 U/μl, NEB), 160 μl Taq DNA ligase (40 U/μl ...
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bioRxiv - Genomics 2022Quote: ... 2 μl of undiluted Thermolabile Proteinase K (NEB #P8111S) and 1 μl SUPERaseIN (20 U/μl ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μl of 10 mM dNTPs (New England Biolabs), 2.5 μl of 10 μM oligonucleotide A (unmodified forward primer ...
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bioRxiv - Biochemistry 2020Quote: ... followed by incubation with 2 Units USER enzyme (NEB) for 10 min at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... for 2 hours or ShortCut RNaseIII (New England Biolabs) for 20 minutes at 37°C after permeabilization before blocking.
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.5 vol 2×Phusion Master Mix (New England Biolabs), and 0.04 vol P1 and P2 amplification primers (10 nm) ...
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bioRxiv - Genomics 2021Quote: ... 2uL 2 000 000U T4 DNA ligase (NEB, M0202). The reaction was incubated at room temperature for 10 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... 1X Phusion buffer and 2 U Phusion Polymerase (NEB). The PCR protocol used was an initial denaturation of 30 s at 98 °C ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μL of 10% NP-40 (NEB Cat # B0701S) and 6 μL of water were mixed with the 10 μL of denatured spike glycoprotein ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 μl NEBuffer 2 (New England Biolabs, Ipswich, USA); 0.3 μl T7E1 enzyme at 10 Units/μl (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... 25 μL NEBNext HiFi 2 × PCR Master mix (NEB) was added ...
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bioRxiv - Genomics 2022Quote: ... 2 µL 400U/µL T4 DNA ligase (NEB, #M0202V), 10 µL 10mM ATP (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2 mM MnCl2 (New England Biolabs, NEB). Control treated cells were incubated with a volume of 50% glycerol equivalent to the volume of RNase III added as well as 2 mM MnCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2 mM MnCl2 (New England Biolabs, NEB). Control treated cells were incubated with a volume of 50% glycerol equivalent to the volume of RNase III added as well as 2 mM MnCl2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 2 µl of 10X ExoI buffer (NEB, B0293S). The annealing was performed in a thermocycler by gradually decreasing the temperature from 98 °C to 60 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... BamHI (Site 2) or HindIII (Control; New England Biolabs) overnight at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 200 U T4 RNA ligase 2 (truncated KQ, NEB) and 40 U Murine RNase Inhibitor (NEB ...
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bioRxiv - Genomics 2024Quote: ... 10 µl of 2% BSA (NEB, B9000S, 0.01% final) and 930 µl of nuclease-free water and supplemented with 1U/ml Protector RNase inhibitor (Millipore Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... containing 6,25µl of LongAmp Taq 2✕ Master Mix (NEB), 4,25µl of milliQ water ...
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bioRxiv - Bioengineering 2023Quote: ... 2 µL of PNGase F (New England Biolabs, MA) was added ...
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bioRxiv - Systems Biology 2024Quote: ... 2 μL of 10x Standard Taq Reaction Buffer (NEB), 0.4 μL of dNTPs (2.5 μM) ...