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Citations for New England Biolabs :
1651 - 1700 of 8855 citations for Soluble Starch Synthase Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... using Q5® Site-Directed Mutagenesis Kit (New England BioLabs, cat # E0554S) with the following primers.
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bioRxiv - Bioengineering 2024Quote: ... gDNA extraction using Monarch® Genomic DNA Purification Kit (New England Biolabs), and histology sectioning.
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bioRxiv - Bioengineering 2024Quote: ... gDNA extraction using Monarch® Genomic DNA Purification Kit (New England Biolabs), and histology sectioning.
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bioRxiv - Biochemistry 2024Quote: ... and ligated together with NEBuilder HiFi DNA Assembly Kit (New England Biolabs). Escherichia coli DH5α competent cells were transformed with the ligation mixtures and the cells containing assembled plasmid were selected on an agar medium supplemented with 100 mg/l ampicillin ...
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bioRxiv - Cell Biology 2024Quote: ... followed by NEBNext Ultra II Direction Prep Kit for Illumina (NEB Cat #). Library sizes was measured using Bioanalyzer and pooled libraries concentration was calculated using NEBNext Lib Quant Kit (NEB Cat#) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR was performed using the Q5 High-Fidelity DNA Polymerase Kit (NEB) and primers specific for the microsatellite expansion adapters (1X Q5 Reaction Buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by the Monarch™ Total RNA Miniprep Kit (New England BioLabs) applying the manufacturer’s protocol for TRIzol® extracted samples ...
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bioRxiv - Developmental Biology 2024Quote: ... was mutated using Q5 site directed mutagenesis kit (NEB, primers: SP (RSV) AP2 (hTFAP2A)-CRISPR RES-fw and SP (RSV ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using NEBNext small RNA sequencing kit (New England Biolabs) or NextFlex small RNA sequencing kit (Perkin Elmer ...
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bioRxiv - Molecular Biology 2023Quote: ... Inserts were acquired by PCR using KOD Hotstart Mastermix from NovaGen and purified from gel using Monarch DNA Gel Extraction kit from NEB. All cloning was performed in E ...
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bioRxiv - Synthetic Biology 2023Quote: ... cDNA was synthesized using a LunaScript® RT SuperMix Kit (NEB E3010), and real time PCR was run on a Bio-Rad CFX96 Touch Real-Time PCR Detection System using an iTaq Universal SYBR Green Supermix (BioRad #1725121) ...
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bioRxiv - Developmental Biology 2024Quote: ... IVT sgRNA products were purified Monarch PCR and DNA Cleanup Kit (NEB) and quantified ...
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bioRxiv - Plant Biology 2023Quote: ... mRNA was purified with the NEB mRNA isolation kit (New England Biolabs). Extracted RNA was treated with DNase I (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... We used a magnetic mRNA isolation kit (New England BioLabs, Ipswich, MA) to enrich samples for mRNA before proceeding to library preparation ...
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bioRxiv - Microbiology 2020Quote: ... total RNA was collected and prepared using Monarch Total RNA Miniprep Kits (NEB). For SARS-CoV-2 experiments ...
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bioRxiv - Plant Biology 2020Quote: ... followed by subcloning into pEAQ-HT vector using the Gibson assembly kit (NEB). Linearization of pEAQ-HT vector was performed by XhoI and AgeI restriction enzyme double digestion ...
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bioRxiv - Plant Biology 2019Quote: ... The libraries were prepared using the Next Ultra RNA Library Prep Kit (NEB) by the company Novogene (China) ...
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bioRxiv - Microbiology 2020Quote: ... chloramphenicol or apramycin resistance cassette by using the Gibson isothermal assembly kit (NEB) followed by PCR amplification using the primers from the extremities ...
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bioRxiv - Microbiology 2020Quote: ... followed by vector-insert ligation using the Quick Ligation kit (New England Biolabs) to generate the original and mutated pCAGGS-HHRz-3M-eGFP-5M vectors ...
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bioRxiv - Cell Biology 2019Quote: ... rRNA was depleted using the NEBNext rRNA Depletion Kit (E6310, New England Biolabs) and subsequently directional RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (E7420 ...
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bioRxiv - Immunology 2021Quote: ... The released DNA was cleaned up using Monarch DNA PCR Clean Kit (NEB) and eluted in TE buffer ...
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bioRxiv - Genetics 2021Quote: SpyCas9 tyr and tbxta sgRNAs were synthesized using EnGen sgRNA Synthesis Kit (NEB). SauCas9 ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplicon was purified using the Monarch PCR and DNA Cleanup Kit (NEB), and 1 ug used as template for transcription of digoxigenin labelled riboprobes using the DIG RNA Labelling mix (Sigma).
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bioRxiv - Genetics 2021Quote: ... The NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) was used for library preparation ...
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bioRxiv - Microbiology 2019Quote: ... and quantified using a NEBNext Library Quant kit for Illumina (New England BioLabs). Pooled libraries were then sequenced on a MiSeq platform (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... rad27 mutant alleles were constructed with the Q5 mutagenesis kit (New England Biolabs) using pEAA720 as template The oligonucleotides used to make the alleles are shown in Table S7) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA libraries were constructed using NEBNext DNA Library Prep Kits (New England Biolabs). Samples were processed and sequenced at Novogene (Hong Kong ...
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bioRxiv - Developmental Biology 2020Quote: cDNA was synthesized using the ProtoScriptII First Strand cDNA synthesis kit (NEB E6560S). Cloning of the full length admats9 from pooled cDNA from 3 ...
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bioRxiv - Developmental Biology 2021Quote: ... They generated mRNA libraries with NEB RNA Ultra kit (New England Biolabs E7770L) and dToligos probe were used to target mRNA for cDNA synthesis ...
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bioRxiv - Bioengineering 2022Quote: ... Amplicons were then indexed using the NEBNext Multiplex Oligos for Illumina kit (NEB). Amplicons were then pooled and sequenced using a Miseq Nano with paired end 150 bp reads ...
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bioRxiv - Molecular Biology 2020Quote: ... by using NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs, E5520S).
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bioRxiv - Molecular Biology 2021Quote: ... The next day samples were purified using PCR cleanup kit columns (Monarch, NEB) and eluted using 50 μl kit elution buffer.
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were prepared using NEBnext Ultra DNA library prep kit for Illumina (NEB). Samples were paired-end sequenced at the iGE3 genomics platform of the University of Geneva.
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bioRxiv - Molecular Biology 2021Quote: ... Illumina libraries were constructed using the NEBNext Ultra II kit (New England Biolabs) and sequenced on an Illumina HiSeq2500 ...
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bioRxiv - Cell Biology 2022Quote: ... and adapter ligation were performed by using RNA library prep kit (E7530L, NEB). DNA products were cleaned using AMPure XP beads (Beckman) ...
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bioRxiv - Genomics 2022Quote: ... DNA was purified using a Monarch PCR & DNA Clean up kit (NEB, T1030) and eluted in 12 μL of water.
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bioRxiv - Genomics 2022Quote: The ligation reaction was carried out using Quick Ligation™ kit (NEB, M2200). A 10 μl mixture consist of 1 μl of 1 μM N40+22nt DNA (0.1 μM final) ...
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bioRxiv - Genomics 2020Quote: ... Libraries were generated using NEBnext Ultra DNA library preparation kit for Illumina (NEB). Libraries were sequenced by paired-end sequencing using a NextSeq 500 (Illumina).
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bioRxiv - Microbiology 2020Quote: ... the source plasmid (p-retronWT) was mutagenized through PCR using a kit (NEB; Q5-Site-Directed Mutagenesis Kit ...
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bioRxiv - Molecular Biology 2021Quote: All mutagenesis was done using Q5 Site-directed Mutagenesis Kit Protocol (NEB #E0554S) according to the manufacturer’s instructions using primers in table T1 ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... gel purifications were performed using the Monarch DNA Gel Extraction Kit (NEB T1020L), and minipreps were performed using the Monarch Plasmid Miniprep Kit (NEB T1010L) ...
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bioRxiv - Molecular Biology 2021Quote: ... NGS libraries were made using NEBNext Ultra II DNA Library Prep kit (NEB). DNA sequencing was performed on Illumina MiniSeq with 150+150 bp paired-end protocol.
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bioRxiv - Cancer Biology 2019Quote: ... Variants were generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). Plasmid transfection was performed with Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... and reverse-transcripted to cDNA using LunaScript RT SuperMix kit (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... PCR was done with the Phusion High-Fidelity PCR kit (New England BioLabs) with 40ng of gDNA ...
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bioRxiv - Plant Biology 2019Quote: TNIC317S active site mutant was generated using Q5 Site-Directed Mutagenesis Kit (NEB).pGEM-T-TNI served as a template for making the site directed mutant using primers P2346 and P2347 ...
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bioRxiv - Genomics 2019Quote: ... the DNA was treated with repair enzymes (New England Biolabs PreCR kit M0309) and purified using Qiagen PCR purification columns as above.
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bioRxiv - Microbiology 2019Quote: cDNA synthesis reactions were carried out using the Lunascript RT Supermix kit (NEB) and 500 ng of RNA was used for each reaction.
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bioRxiv - Evolutionary Biology 2019Quote: ... CPS mutant plasmids were constructed using the Q5 Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Cancer Biology 2019Quote: ... SPA4CT-T43P was generated using the Q5 Site-Directed Mutagenesis Kit (NEB E0554S) using pLVX SPA4CT as a template ...