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Citations for New England Biolabs :
1651 - 1700 of 4504 citations for Integrin beta 1 binding protein 1 ITGB1BP1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Unmethylated λ-bacteriophage DNA (1:200 mass ratio; New England Biolabs) was included in all samples to calculate unmethylated cytosine conversion efficiency ...
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bioRxiv - Microbiology 2024Quote: ... 1 µl (500 Units) of PNGase F (New England BioLabs, Inc.) was used to deglycosylate 10 µg of the protein in the presence of 5 mM DTT and mild detergents by incubating at room temperature for 1 hour according to manufacturer’s recommendations.
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bioRxiv - Microbiology 2024Quote: ... respectively) using T4 RNA Ligase 1 (New England Biolabs, USA; #M0204) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µl of Golden Gate Enzyme Mix (BsaI-HFv2, NEB M2616AA), and adding dH2O to reach a total volume of 20 µl ...
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bioRxiv - Microbiology 2024Quote: ... Ligation of 5’ linker using T4 RNA ligase 1 (NEB; #M0204S) was conducted after phosphorylating the 5’ end of the pooled fragments with T4 PNK (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and 1 μL of terminal transferase (20 units, NEB Catalog # M0315S) was added to the reaction with incubation at 37 °C for 1 h to block any pre-existing strand-break sites ...
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bioRxiv - Neuroscience 2024Quote: ... brains were incubated in SNAP-Surface 647 (1:1000; NEB; S9136S) or CLIP-Surface 647 (1:1000 ...
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were then washed twice in 1× rCutSmart buffer (NEB; #B6004S) and twice in 1×T4 ligase buffer (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 U of α2-3,6,8,9 neuraminidase (NEB; Cat. No. P0722L), 50 mM phosphate buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 72 °C for 1 min) × 5] using NEBNext 2xMasterMix (NEB, M0541S) with Ad1_noMX and v2_Ad2.* indexing primers followed by qPCR amplification to determine additional cycle numbers ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mM ATP with 20 U of T4 PNK (NEB, M0201). Following an ethanol precipitation ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 µL of Thermostable RNase H (New England Biolabs, M0523S) before incubation at 55 °C for 15 minutes ...
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bioRxiv - Genetics 2024Quote: ... and 1 µL CutSmartTM r3.1 Buffer (New England BioLabs, MA, USA) was incubated for 2 hours at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... a respective combination of 1 µL FPG (NEB, 8 U/µL), 1 µL ENDOIV (NEB ...
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bioRxiv - Genomics 2024Quote: ... 1 µL T4 polynucleotide kinase (T4 PNK) (10 U/µL, NEB) was added to the gDNA in 1x NEBuffer 2 (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... with or without 1 µL/reaction of PNGase F (NEB, P0704S) and incubated for 1 h at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... DNAs were incubated with 1 µl EcoRI in Buffer 2.1 (NEB). Reaction mixtures were treated with Proteinase K for 30 minutes at 55°C and products were separated by electrophoresis on 1xTBE 15% polyacrylamide gels ...
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bioRxiv - Genomics 2024Quote: ... using the 1 kb plus DNA ladder (New England Biolabs, N3200S). High molecular weight samples were retained for subsequent steps ...
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bioRxiv - Genomics 2024Quote: ... DNA was mixed with 1 µL FPG (NEB, 8 U/µL) and 1 µL T4 PNK (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by the addition of 1 μl M13KO7 helper phage (NEB).
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bioRxiv - Cancer Biology 2024Quote: ... made up of 1 uL DNA Ladder (New England Biolabs, N3231L), 1 µL Purple Dye (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... 72 °C for 1 min) × 5] using NEBNext 2xMasterMix (NEB, M0541S) with Ad1_noMX and v2_Ad2.* indexing primers followed by qPCR amplification to determine additional cycle numbers ...
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bioRxiv - Systems Biology 2024Quote: ... IVT products were treated with 1 U of DNase I (NEB) at 37°C for 15 min and brought to a final volume of 100 µL with nuclease-free water ...
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bioRxiv - Microbiology 2024Quote: ... (b) Heat labile Shrimp Alkaline Phosphatase (rSAP) (1 µl) (NEB #M0371S) was used to remove terminal phosphates that could interfere with terminal extension by incubating in rCutSmart buffer (3.5 µl ...
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bioRxiv - Microbiology 2024Quote: ... and 1 μL of terminal transferase (20 units, NEB Catalog #M0315S) was added to the reaction with incubation at 37 °C for 1 h to block any pre-existing strand-break sites ...
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bioRxiv - Molecular Biology 2024Quote: ... steps 1–3: GELase was replaced by β-agarase I (NEB). The reaction (1 U per plug ...
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bioRxiv - Bioengineering 2024Quote: ... NEBNext Multiplex Oligos for Illumina (Index Primer Set 1) (NEB, 7335S) were used for the final PCR amplification ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µl T4 DNA ligase (400 units/µL; New England Biolabs), and 1.5 µl 10x T4 DNA ligase buffer ...
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bioRxiv - Bioengineering 2024Quote: ... 1 U/µL Murine RNAse Inhibitor (New England Biolabs, Cat #M0314L), 0.002 U/µL yeast inorganic pyrophosphatase (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli NGF-1 gDNA using Q5 High-Fidelity PCR polymerase (NEB) following the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2024Quote: ... 1 unit (100nM) Casein Kinase II (CK2) (New England Biolabs, P6010SVIAL) and/or recombinant human GSK3 beta (Abcam ...
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bioRxiv - Bioengineering 2024Quote: ... and 1 µg of gDNA per sample was DpnI-digested (NEB) to remove residual donor plasmid ...
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bioRxiv - Cancer Biology 2024Quote: ... Primers Set 1 and 2 (NEB, cat. no. E7335 and E7500). We proceeded by pulling the samples together ...
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bioRxiv - Genetics 2024Quote: ... Reactions were incubated with 1 µL Themolabile Exonuclease I (NEB, M0568L) at 10 min at 37° ...
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bioRxiv - Cell Biology 2024Quote: ... reverse-transcribed (1 μg, LunaScript RT Supermix 5X; New England Biolabs), and analyzed by quantitative PCR (qPCR ...
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bioRxiv - Genetics 2023Quote: ... We added phasing nucleotides as well as overhangs for indexing primers using primer mixtures SL5.F[1-4] and SL5.R[1-4] (NEB Q5 for 20 cycles, Tm 62 °C). We finally added dual indexing primers using the i5 and i7 system from Illumina (NEB Q5 for 20 cycles ...
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bioRxiv - Biochemistry 2020Quote: ... 50 μg of purified protein complexes were treated with 2,000 U of Lambda Protein Phosphatase (New England Biolabs) in 200 μl dephosphorylation buffer (50 mM HEPES-NaOH ...
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bioRxiv - Systems Biology 2023Quote: ... the wild-type protein extracts were incubated with 40 units of Lambda Protein Phosphatase (New England Biolabs, P0753S) at 30°C for 30min ...
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bioRxiv - Cell Biology 2024Quote: ... Protein lysates (500 µg) were subjected to precleaning using 25 µl Protein A (New England Biolabs, Cat # S1425S) and G (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... in protein kinase buffer (New England Biolabs) in the presence of 0.2 mM Mg2+-ATP and 1 µCi [γ32-P]ATP for 30 min at 30°C ...
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bioRxiv - Cell Biology 2020Quote: ... in protein kinase buffer (New England Biolabs) and 0.5 mM Mg2+-ATP for 30 min at 30°C ...
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bioRxiv - Cell Biology 2020Quote: ... Protein Phosphatase Inhibitor 2 (New England Biolabs) and Trichostatin A HDAC inhibitor (TSA ...
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bioRxiv - Physiology 2022Quote: ... The Cas9 protein was purchased from NEB. Mixture of sgRNA (100 pg ...
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bioRxiv - Immunology 2022Quote: ... 1μl of Lambda Protein Phosphatase (NEB, P0753S) was added and samples were incubated at 30°C for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... protein tyrosine phosphatase (PTP, 5 unit; NEB), or sodium orthovanadate (Na3VO4 ...
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bioRxiv - Plant Biology 2021Quote: ... Lambda protein phosphatase treatment (New England BioLabs) was performed following the manufactorer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Lambda protein phosphatase (New England BioLabs, P0753S) was used per product instructions.
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bioRxiv - Pathology 2021Quote: Magnetic protein G beads (New England Biolabs) were blocked in antibody selection buffer and used to immobilize 10-15μg polyclonal rabbit anti-VWF (Dako A0082 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were degraded using proteinase K (NEB) and RNA was purified using RNeasy maxi kit (Qiagen).
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bioRxiv - Microbiology 2023Quote: ... coli Protein Synthesis System (New England Biolabs), then purified with Ni-NTA beads (Qiagen ...