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Citations for New England Biolabs :
1651 - 1700 of 3961 citations for Huntingtin Interacting Protein 1 Related Protein HIP1R Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... 1.5 μL 1:1,249 diluted Fe2+ solution (NEB), and 8.4 μL water ...
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bioRxiv - Molecular Biology 2023Quote: ... MA) in 50 µl 1× CutSmart buffer (NEB), 120 min at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... coli RNAP holoenzyme from NEB (1 U/µl) was used at 1/3 dilution ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µg pKHH030 was digested with BbsI (NEB) in CutSmart Buffer (NEB ...
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bioRxiv - Genomics 2023Quote: ... 1 uL of QuickCIP (New England Biolabs #M0525S) was added directly to the backbone digestions and incubated at 37°C for 30 minutes ...
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bioRxiv - Genomics 2023Quote: ... was pre-incubated in 1× NEBuffer 3.1 (NEB) with 25 pmol sgRNA at 37°C for 15min to form Cas9-Ribonucleoprotein complex ...
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bioRxiv - Genomics 2023Quote: ... 3 μL 1:1,249 diluted Fe2+ solution (NEB)) at 37°C for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µL 1U recombinant shrimp alkaline phosphatase (NEB) and 2.5 µL 10x CutSmart buffer (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... or with XRN-1 and RppH (NEB #M0356S) together ...
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bioRxiv - Developmental Biology 2024Quote: ... treated with Proteinase K (1:10000) (P8108S, NEB) for 2 min ...
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bioRxiv - Biochemistry 2024Quote: ... 5 U µL-1 T7 RNA polymerase (NEB) and 0.2 µg µL-1 template DNA ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 U/µL T4 RNA ligase 1 (NEB) and 10 U/µL T4 RNA ligase 2 ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... 1 ul of T5 Exonuclease (New England BioLabs) was added to the samples for 1 hr on a 37 degrees Celsius shaker ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 μl RppH 5000 U/μl (NEB) was added to the RNA/oligo mix ...
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bioRxiv - Cancer Biology 2024Quote: ... TOM20 at 1:1000 (New England Biolabs 42406S) were prepared in TBST with 3% bovine serum albumin (BSA) ...
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bioRxiv - Molecular Biology 2020Quote: ... m6A pulldown was performed with a rabbit monoclonal anti-m6A antibody in the EpiMark N6-Methyladenosine Enrichment Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Antibody de-N-glycosylation was carried out by incubating the sample with PNGase F (New England Biolabs, Ipswich, MA) for 24 hrs at 37 OC (enzyme:substrate ratio ca ...
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bioRxiv - Microbiology 2020Quote: ... 3 μL of in vitro transcribed 300 nM sgRNA or trL (produced as described in in vitro transcription protocol above) and 1 μL of 1 μM Cas9 nuclease (NEB, M0386S) were mixed and incubated for 10 minutes at 25°C to allow for RNP formation ...
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bioRxiv - Cell Biology 2021Quote: ... 1% NP-40, 1 mM DTT, 0.1 mg/ml cycloheximide, 1.25 mg/ml heparin, 400 U/ml NEB murine RNase inhibitor) and dounced ...
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bioRxiv - Plant Biology 2021Quote: ... and cloned into Gateway™ pENTR™ 4 by mixing the linearized vector backbone and PCR product in a 1:1 ratio using Gibson assembly (NEB), before transformation into DH10B electro-competent E ...
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bioRxiv - Genomics 2022Quote: ... Illumina libraries and DNA splint were mixed at a 1:1 ng ratio using NEBuilder HiFi DNA assembly Master mix (NEB #E2621). Any non-circularized DNA was digested overnight using ExoI ...
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bioRxiv - Microbiology 2020Quote: ... The flies were homogenised in 100 μl of TE-buffer pH 8 containing 1% Triton X-100 and 1% Proteinase K (NEB, P8107S). Homogenates were incubated for 3 h at 55°C followed by a 10 min incubation step at 95°C ...
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bioRxiv - Immunology 2022Quote: ... cleavage rates were transformed into standardized catalytic activities (1 “eqU” = equivalent to the catalytic activity of 1 U RNase HII (NEB ®) under standard conditions) ...
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bioRxiv - Systems Biology 2019Quote: ... the Nickel-NTA beads were incubated in 80 μl 3’-linker ligation mix with (1 X PNK buffer, 1 µM 3’-adapter, 10% PEG8000, 30U Truncated T4 RNA ligase 2 K227Q (NEB, M0351L), 60U RNasin) ...
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bioRxiv - Molecular Biology 2020Quote: ... was linked to the free hydroxyl group at the 3’-end of transcripts (1 □g of total RNA) by T4 RNA ligase 1 (NEB M0204) in the presence of 15% (w/v ...
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bioRxiv - Bioengineering 2022Quote: ... 1X of a restriction enzyme mix [1/2 EcoRI-HF® (NEB #R3101, 2/5 Nuclease-free water, 1/10 CutSmart Buffer 10X (NEB)] ...
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bioRxiv - Genomics 2023Quote: ... cells were finally resuspended in 400ul PSB-DTT at the concentration of ∼2e6 cells/100ul (PBS, 1% SUPERase In, 1% BSA (NEB B90000S), 1mM DTT) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were live labeled with 1 μM ATTO 590-chloroalkane and 1 μM of SNAP-Cell 647-SiR (New England Biolabs; S9102S) (final concentrations) ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... RT-PCR or qPCR was performed with 1 μl of 1:2 diluted cDNA using the Q5®Hot Start High-Fidelity 2X Master Mix (NEB) or the SYBR™ Green PCR Master Mix (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... Insert and vector were combined at a 5:1 ratio and held at 50 °C for 1 hour in NEBuilder® HiFi DNA Assembly Master Mix (NEB). Gibson assembly products were purified using the MinElute® PCR Purification Kit (Qiagen) ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... top and bottom oligonucleotides for a single R-S-R or dead end oligonucleotide pairs were combined 1 µL:1 µL and were phosphorylated in a 50 µL reaction using T4 Polynucleotide Kinase (NEB M0201L) for 2 h at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: For proximity ligation, the pellet was resuspended into 500 μL of ligation mix (1× T4 DNA ligase buffer, 1× BSA (NEB, B9000S), 5000 CEU T4 DNA ligase (NEB ...
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bioRxiv - Cancer Biology 2020Quote: ... and murine lung slices were incubated overnight with primary antibodies in 0.1% BSA as follows: anti-CDH1 (clone EP700Y, Biozol, 1:250) or anti-CDH1-Alexa-488 (clone 24E10, New England Biolabs, 1:50), anti-Twist1 (clone Twist2C1a ...
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bioRxiv - Microbiology 2021Quote: ... the purified cDNA was PCR amplified and barcoded using ONT’s PCR Barcoding Expansion 1-12 (EXP-PBC001) or PCR Barcoding Expansion 1-96 (EXP-PBC096) kits with LongAmp Taq 2x Mastermix (NEB, Ipswich, MA).
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bioRxiv - Microbiology 2020Quote: ... 50µl of lysed cells were transferred to a tube containing 450µL of digestion mix (1X NEB 1 buffer, 1% triton X-100). This process was repeated 11 times (total 12 tubes with 500 µl) ...
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bioRxiv - Biochemistry 2021Quote: ... the receptor was truncated at C-terminus at two positions (i.e. 1-342 and 1-351) by inserting a STOP codon by site-directed mutagenesis (NEB, Cat. no. E0554). The surface expression of WT and truncated receptor constructs were normalized to similar levels by DNA titration in HEK-293 cells ...
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bioRxiv - Genomics 2019Quote: ... expressing ATG7 isoform 2 was a gift from Toren Finkel (Addgene plasmid #24921).31 The plasmid pCMV-myc-Atg7(1) expressing ATG7 isoform 1 was derived from pCMV-myc-Atg7(2) using the Q5 site-directed mutagenesis kit (NEB, Ipswich, MA). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were visualized on a 1% agarose gel using the Quick-load Purple 1-Kb DNA ladder (New England Biolabs, Ipswich, MA). Primer3 Plus (Untergasser et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed in 1 mL ice-cold RNase-free cell lysis buffer supplemented with 1 μL RNase inhibitor (M0307S, NEB; Ipswich, MA). After storage in −80°C refrigerator for about 40 mins ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated with mouse anti-CaMKIIα (6G9) (1:1000, Pierce Biotechnology, Catalog #MA1-048) and rabbit anti-Gaussia luciferase (1:1000, New England BioLabs, Catalog #E8023S) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... μL supernatant were incubated with biotinylated DNA that was immobilized to streptavidin magnetic beads (1 pmol DNA to 1 μL beads; New England Biolabs, Ipswich, MA), as described in Meinhardt et al.79 ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibodies used were: Alexa-680 goat anti-mouse IgG and Alexa-800 goat anti-rabbit IgG (New England Biolabs). For DRGN cultures ...
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bioRxiv - Neuroscience 2021Quote: ... was separated into low-protein absorption tubes (PROKEEP; Watson Bio Lab, Tokyo, Japan) and reacted with 2 μg of rabbit polyclonal anti-lactyllysine antibody (PTM Biolabs) or normal rabbit IgG (Cell Signaling Technology ...
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bioRxiv - Microbiology 2021Quote: ... and either stored at −20°C or 10 μg used for immunoblots with an Anti-IkBa antibody (New England Biolabs) (54).
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bioRxiv - Cell Biology 2021Quote: ... Samples were resolved by SDS-PAGE in urea loading buffer and analysed by Western blot against the SNAP-tag (rabbit polyclonal anti-SNAP-tag antibody P9310S, New England Biolabs) to detect levels of SNAP-GLP-1R in the different fractions ...
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bioRxiv - Biochemistry 2020Quote: ... pCSE2.6-hFc-XP or pCSE2.6-mFc-XP 68 where the respective single chain variable fragment of the antibodies or antigens were inserted by NcoI/NotI (NEB Biolabs) digestion ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were separated through SDS-PAGE and transferred to PVDF membrane followed by incubation with anti-MBP monoclonal antibody (E8032S, NEB) and M2 Flag antibody (A8592 ...
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bioRxiv - Immunology 2021Quote: The cDNA sequences of the paired variable heavy and light chain region of anti-RBD antibody clones were synthesized as gBlocks (IDT) and cloned by the Gibson assembly (NEB) into human IgG1 heavy chain and light chain expression plasmids ...
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bioRxiv - Microbiology 2023Quote: Unique VH and VL domains were cloned into linearized human antibody expression vectors (human IgG1 and kappa light chain) using Gibson assembly (NEB) according to the manufacturer’s directions ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were separated through SDS-PAGE and transferred to a PVDF membrane followed by incubation with anti-MBP monoclonal antibody (E8032S, NEB) and M2 Flag antibody (A8592 ...