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Citations for New England Biolabs :
1651 - 1700 of 3658 citations for Diethyl 2 chlorothiazol 5 yl methylphosphonate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... We performed a second bead binding followed by a 5’ decapping with RppH (NEB, M0356S). RNA was phosphorylated on the 5’ end using T4 polynucleotide kinase (NEB ...
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bioRxiv - Genomics 2022Quote: ... 5 μl of highly concentrated HhaI enzyme (150,000 U/mL, NEB; Cat. Num. R0139B-HC1), while keeping the remaining reagents as stated in the Tapestri protocol (5 μl Forward Primer Pool ...
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bioRxiv - Cell Biology 2021Quote: ... A-tailing 3’ end was performed using Klenow Fragment (3’→5’ exo-) (New England Biolabs), and then TruSeq Adapters were ligated by Quick T4 DNA Ligase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 0.1 mM dATP and 15 units of Klenow Fragment (3’→5’ exo-) (NEB, M0212M) and incubated at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: 5’ linker ligation (1x PNK buffer, 40 U T4 RNA ligase I (NEB, Cat# M0204L), 80 U RNaseOUT ...
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bioRxiv - Microbiology 2022Quote: ... CrPV 5’UTR-1A-GFP-3’ UTR was generated using Gibson assembly (NEB Gibson assembly). The respective mutants were generated using Site directed mutagenesis ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μl of 2x Quick Ligase buffer and 1 μl of Quick ligase (NEB, M2200) in a final volume of 10 μl was incubated for 10 min at RT and then used to transform DH5α competent cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Phosphorylation of the 5’ end using T4 polynucleotide kinase (New England Biolabs, Ipswich, MA, M0201L) (iii ...
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bioRxiv - Molecular Biology 2020Quote: ... 240 μM TruSeq Universal Adapter (5’-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3’) and 0.025 U Taq DNA Polymerase (NEB) in 1X Standard Taq Reaction Buffer (NEB) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Hemiligated DNA was 5’-phosphorylated with T4 PNK in T4 DNA Ligase Reaction Buffer (NEB) at 25°C for 30 minutes ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli using standard transformation techniques with NEB 5-Alpha chemically competent cells (New England BioLabs), and to C ...
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bioRxiv - Biophysics 2021Quote: ... 5 μL proteoliposomes were mixed with 8 units of enterokinase light chain (New England Biolabs) and diluted to 20 μL ...
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bioRxiv - Physiology 2020Quote: ... and 5 μg of the vector was linearized using restriction enzymes (NEB, Hitchin, Hertfordshire, UK). Linearized vector containing the ArCCKP cDNA were cleaned using phenol-chloroform (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μl RT mastermix (2x reaction buffer, 20 mM DTT, 4U murine RNase inhibitor [NEB] ...
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bioRxiv - Microbiology 2021Quote: ... 5μM diphosphorylated 20mer RNA substrate was pre-treated with 0.5U/μl RNA 5’ Pyrophosphohydrolase (NEB) in 1× NEBuffer 2 (NEB ...
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bioRxiv - Genetics 2020Quote: ... The 10 μl reactions contained 5 μl Q5 High-Fidelity Master Mix (New England Biolabs), 1 μl forward primer [10 μM] ...
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bioRxiv - Molecular Biology 2020Quote: ... and 2.0 units of Klenow Fragment (3’-5’ exo−)(New England Biolabs, Ipswich, Massachusetts, USA), total 10 μl of the mixture was incubate at 37°C for 90 min ...
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bioRxiv - Synthetic Biology 2023Quote: All cloning was performed in NEB 5-alpha Competent Escherichia coli (New England BioLabs C2987U). The Pseudomonas putida KT2440 strain was purchased from ATCC (#47054) ...
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bioRxiv - Microbiology 2023Quote: ... Oligos included 5’ overhangs complementary to the sticky ends generated by BsaI-HF (NEB, R3733S) restriction enzyme ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.01% Igepal CA-630 and 5% glycerol) with 1 U/µL murine RNase inhibitor (NEB), 0.1 µg/µL T7 RNA polymerase (purified in-house ...
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bioRxiv - Genomics 2023Quote: ... the single-stranded probes were further digested with 5 units of USER enzyme (NEB M5505L) in 1x DpnII buffer at 37 °C for 3 hours.
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bioRxiv - Biophysics 2023Quote: ... Calmodulin and myosin-5 expression plasmids were prepared from E.coli 5α cells (New England Biolabs), transformed with the each of plasmids ...
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bioRxiv - Molecular Biology 2023Quote: ... 6 μL 20mg/ml BSA and 5 μL 400U/μl T4 DNA Ligase (NEB, M0202) overnight at 16°C (tumbling ...
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bioRxiv - Molecular Biology 2023Quote: ... Small RNAs were 3’ dephosphorylated and 5’ phosphorylated with T4 polynucleotide kinase (T4PNK, NEB, #M0201S), and purified with RNA Clean and Concentrator-5 (Zymo Research ...
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bioRxiv - Immunology 2023Quote: ... The diluted supernatant was loaded onto a 5 mL amylose resin column (New England Biolabs). The column was washed with 150 mL of buffer I ...
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bioRxiv - Neuroscience 2023Quote: ... 5-HT2A receptor mutants were designed and performed using Q5 mutagenesis kit (New England Biolabs). All DNA was sequence verified using Sanger nucleotide sequencing (Retrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... The assembled plasmid was introduced into Escherichia coli strain NEB 5-alpha (New England Biolabs) by heat shocking ...
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bioRxiv - Microbiology 2023Quote: ... The resultant insert (5′-flank_SpecPromoter_kanR_3′-flank) was gel extracted (Monarch DNA GEL Extraction Kit, NEB) and added to competence peptide stimulated S ...
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bioRxiv - Cancer Biology 2023Quote: ... Exonuclease treatment was performed by addition of 5 µL of NEBbuffer1 (NEB, cat. no. B7001S) and 1 µL of each Exonuclease I (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μM Rad51 and 10 μM (nucleotide) φX174 circular single-stranded DNA (ccsDNA) (N3023L, NEB) were mixed in reaction buffer (25 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and second strand cDNA synthesis (using Klenow fragment 3’-5’ exo- [New England BioLabs, USA]) of chicken fecal samples and dust samples was performed as previously described 55 and following manufacturer’ instructions.
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bioRxiv - Plant Biology 2022Quote: ... 5 pmol of label-free RNA substrates were dephosphorylated with Alkaline Phosphatase (New England Biolabs) and labeled with [γ-32P] ATP (PerkinElmer ...
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bioRxiv - Cancer Biology 2022Quote: ... 3 μg purified DARPP-32 isoforms as well as 5 μl ATP (New England Biolabs) in kinase dilution buffer III (SignalChem ...
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bioRxiv - Cell Biology 2022Quote: ... A18-Y260A-SDM-Rev (5’-CGTTGTGTAACCAGGAGAATG-3’) and Q5 site directed mutagenesis kit (NEB E0554). This LT3N1-GPIR-Arhgef18 vectors were further modified to substitute EGFP with 3XHA-FLAG tag ...
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bioRxiv - Cell Biology 2022Quote: ... miRE-Rv (5’-TCTCGAATTCTAGCCCCTTGAAGTCCGAGGCAGTAGGC-3’) and Q5 Hot Start High-Fidelity DNA polymerase (NEB M049S). The final amplicon was then digested and cloned into LT3GEPIR in-between XhoI and EcoRI restriction sites as previously described2 ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing Cdt1 were further purified on a 5 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was further purified on a 5-10 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Cancer Biology 2024Quote: ... All ngRNAs were cloned into pLKO.5 Puro-2A-RFP between BsmBI (New England Biolabs) cut sites ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The 10-µl reactions contained: 5 µl Luna Universal qPCR Master Mix (New England Biolabs), 0.25 µl forward primer (10 µM) ...
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bioRxiv - Biochemistry 2024Quote: ... and the PCR products were subsequently 5’ phosphorylated using T4 polynucleotide kinase (New England Biolabs) and self-ligated by T4 DNA ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µl of LongAmp Taq 2x master mix (New England Biolabs, Ipswich, Massachusetts, United States), and 2.5 µl of the PCR product as template ...
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bioRxiv - Plant Biology 2024Quote: ... dephosphorylated F4 (5 µM) was incubated with γ-[32P]-ATP and T4 polynucleotide kinase (NEB) for 45 min at 37°C ...
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bioRxiv - Genomics 2024Quote: ... DNA was circularized at a concentration of 5 ng/µl with T4 DNA ligase (NEB), and treated with a cocktail of exonucleases ...
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bioRxiv - Molecular Biology 2023Quote: ... 55 Excess adaptor was removed by incubation with 5′ deadenylase and the exonuclease RecJf (NEB). TIS11B protein-RNA complexes were eluted from the beads by heating at 70°C for one minute ...
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bioRxiv - Cancer Biology 2023Quote: ... 1pmol ssDNA substrate 5’ DY782 ATTATTATTATTATTATTATTTCATTTATTTATTTATTTA-3’ (Eurofins, UK) and 0.75U uracil-DNA glycosylase (NEB) were added to 10µg protein lysate at 37°C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Nef deletion Reverse: 5’-AGATCTACAGCTGCCTTGTAAGTCATTGG-3’) using Q5® Site-Directed Mutagenesis Kit (NEB #E0554S).
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bioRxiv - Molecular Biology 2023Quote: ... Probes were synthesized as ssDNA oligos and 5’ 32P-radiolabeled with T4 polynucleotide-kinase (NEB) and ψ-32P-ATP (6000 Ci/mmol ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′ Iq competent Escherichia coli (NEB, Cat# C2992H) and extracted using the PureYield Plasmid Miniprep System (Promega ...
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bioRxiv - Molecular Biology 2023Quote: RNA was denatured for 5 min at 95°C in 1x RNA Loading Dye (NEB) and separated on denaturing polyacrylamide gels (12% ...
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bioRxiv - Cell Biology 2023Quote: ... using 5 μl of the reaction with 1 μl of loading dye (New England Biolabs). Agarose gel was electrophoresed at 70V until resolution of bands required was reached ...