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Citations for New England Biolabs :
1651 - 1700 of 2930 citations for Acyl protein thioesterase 2 LYPLA2 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... SNAP-FOR1(3P,FH2) protein was labeled with SNAP-549 dye (New England Biolabs, Ipswich, MA) as per manufacturer’s instructions prior to each TIRF experiment.
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bioRxiv - Cell Biology 2020Quote: Total protein extracts were obtained from cell pellets directly resuspended in the loading buffer (Biolabs B7709S) containing 1% SDS ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were prediluted to a concentration of 750 nM in 1x CutSmart buffer (New England Biolabs) and preloaded with guide DNA or guide RNA in a 1:1 ratio at 37°C for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... All proteins were overexpressed in Escherichia coli BL21 Codon-Plus competent cells (C2527, New England Biolabs) and grown at 37 °C in 2 % Luria Broth containing 30 mg/L kanamycin until OD600 reached 0.6 ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were injected with 200-300 pg gRNAs and 1.6 ng EnGen Cas9 protein (#M0646M NEB). The target and gRNA sequences ...
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bioRxiv - Molecular Biology 2021Quote: ... The antibodies were captured using 50 µl of protein G magnetic beads (S1430, New England Biolabs) incubated for 60 min with end over end mixing at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein and non-incorporated nucleotides were removed using the Monarch RNA Clean up kit (NEB, T2040), before quantification by nanodrop.
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bioRxiv - Microbiology 2022Quote: All recombinant proteins were expressed in Escherichia coli T7 Express lysY/Iq cells (New England Biolabs). Except for GST-PP1γ ...
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bioRxiv - Biochemistry 2022Quote: ... CBP (Chitin Binding Protein, produced by N. Martín in Dr. Casanova’s lab, New England Biolabs Protocol) was used as a secondary antibody at 1:300 to detect chitin and visualize the tracheal branches ...
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bioRxiv - Microbiology 2022Quote: ... individual sgRNAs and Cas9 protein in 1X NEB 3.1 buffer (New England Biolabs, Ipswich, MA, USA) were incubated at 37°C for 2 h ...
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bioRxiv - Microbiology 2022Quote: ... The DST was removed by incubating the protein with 64 units of Enterokinase light chain (BioLabs) in 10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: Genetically modified zebrafish lines were created using Cas9 protein (EnGen® Spy Cas9 NLS, #M0646T, NEB) and previously established protocols combining PCR and reverse transcription to generate guide RNAs44 ...
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bioRxiv - Microbiology 2022Quote: ... The EcRecA protein was purified in a similar manner and then compared to commercial EcRecA (NEB). The protein activity of commercial NEB and purified proteins was equivalent ...
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bioRxiv - Microbiology 2024Quote: ... thermophilus extracts or PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, Ipswich, MA, USA) and the reactions were further incubated at either 50 °C or 37 °C for another 4 h in the case of T ...
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bioRxiv - Molecular Biology 2024Quote: The glycosylation status of the proteins was studied using the PNGase-F and the EndoH (NEB) glycosidases according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples collected from culture media were treated with PNGase F enzyme (New England Biolabs, P0709S) to remove the N-linked oligosaccharides from the TECTA protein according to the manufacturer protocol ...
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bioRxiv - Biochemistry 2024Quote: ... crude extracts were treated with the λ-protein phosphatase (λ-PPase; New England BioLabs, Cat# P0753S) before application on the gel ...
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bioRxiv - Bioengineering 2024Quote: ... In vitro cleavage reactions were performed on the circularized DNA using SpCas9 protein (NEB, Ipswich, MA) and KLRC1 sgRNA ...
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bioRxiv - Cell Biology 2023Quote: ... In some cases immunoprecipitated proteins were treated with PNGase-F or Endo-H (New England Biolabs) according to the manufacturers protocols ...
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bioRxiv - Microbiology 2023Quote: ... The HA protein tag and point mutations were introduced using the KLD Enzyme mix (NEB M0554S) and were verified by Sanger sequencing and whole-plasmid sequencing (Plasmidsaurus).
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bioRxiv - Biochemistry 2023Quote: ... per 1 µg protein for 45 min at 30°C in PMP buffer (New England Biolabs) plus 1 mM MnCl2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Preclearing step: ∼ 50 μL magnetic beads (Protein A or G Magnetic Beads; #S1425S or #S1430S NEB) were added to the sample and incubation was carried out for 1 h on a rocking platform at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate-antibody mix was then incubated with Magnetic Protein A beads (New England Biolabs, S1425S) overnight at 4 °C with end over rotation ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by simultaneous overnight tag cleavage by TEV and dephosphorylation by λ-protein phosphatase (NEB, P0735). The cleaved ...
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bioRxiv - Biophysics 2023Quote: ... The labelled proteins were subjected to overnight cleavage by exogenous furin (New England Biolabs, Ipswich, MA) at 37°C to fully convert GP0 to GP1 and GP2 ...
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bioRxiv - Neuroscience 2024Quote: ... and used to form ribonucleoprotein (RNP) complexes when combined with recombinant Cas9 protein (New England Biolabs). Ribonucleoprotein complexes were formed using the method of Kouranova49 ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were eluted with lysis buffer + 250 mM imidazole and bound to chitin-resin (S6651L, NEB), before washing in lysis buffer and overnight incubation with lysis buffer + 200 mM DTT and his-ULP1 protease ...
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bioRxiv - Biochemistry 2024Quote: ... reactions were performed with 6 μl of PURExpress in vitro protein synthesis system (New England Biolabs). The reactions were carried out on different templates (Supplementary Table 2) ...
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bioRxiv - Bioengineering 2024Quote: Protein was expressed for in vitro assays in T7 Express cells (New England Biolabs, Catalog # C2566H) in 96-well deep-well plates using the pET22b(+ ...
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bioRxiv - Cell Biology 2024Quote: ... AcGFP and TagRFP-657 proteins and SNAP-Surface 488 and Alexa 647 dyes (New England Biolabs) were measured in the same excitation and emission ranges as in flow cytometry assays ...
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bioRxiv - Molecular Biology 2020Quote: ... The mixed RNA was depleted of rRNA using the Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) and fragmented using NEBNext® Magnesium RNA Fragmentation Module (NEB, cat. no. E6150S) for 5 min ...
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bioRxiv - Genetics 2021Quote: ... 0.5 μM MPRA_v3_F and MPRA_v3_20I_R primers (Supplementary Table 14) and 2 ng BSA (NEB, B9000). PCR master mix was emulsified by vortexing with 220 μL Tegosoft DEC (Evonik) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... treated ~2 μg RNA with RNase-free DNase I (Catalog# M0303S NEW ENGLAND Biolabs, USA), then used 1 μL treated RNA in cDNA synthesis with SuperScript III Reverse Transciptase (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro was opened with BsrGI-HF (NEB) and EcoRI-HF (NEB) ...
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bioRxiv - Developmental Biology 2022Quote: ... samples were washed in PBS-T and blocked in PBS-T + 2% BSA (NEB, B9000) + 5% donkey/goat serum (Jackson Immunoresearch/Dianova ...
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bioRxiv - Molecular Biology 2020Quote: ... or 500 ng of chromatin-bound or nucleoplasmic RNA with 2 µl Quick CIP (NEB) in a total volume of 20 µl for 90 min at 37°C ...
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bioRxiv - Genomics 2020Quote: ... add 2 μl 10 mM dATP and 5 μl Klenow Fragment (3’ −> 5’ exo-) (NEB) to digest the unligated DNA fragments at 37°C for 40 min ...
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bioRxiv - Genomics 2019Quote: ... NEBNext Multiplex Oligos for Illumina (NEB, Set 1; cat. # E7335, NEB, Set 2; cat. # E7500) were used for multiplexing ...
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bioRxiv - Bioengineering 2019Quote: ... 5 μL of 10 × GlycoBuffer 2 (supplied by NEB, 500 mM Sodium Phosphate, pH 7.5), and 1 μL of PNGase ...
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bioRxiv - Biochemistry 2020Quote: ... pre-tRNA stock was diluted 1 in 2 into RNA loading buffer (New England Biolabs) and separated on a 10% acrylamide urea-TBE denaturing gel ...
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bioRxiv - Immunology 2021Quote: ... PCRs were performed with Phusion High-Fidelity DNA Polymerase (2 U/µL) (New England Biolabs) in a total volume of 50 μl ...
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bioRxiv - Genomics 2020Quote: ... 5 μL of Phusion High Fidelity 2× Master mix (New England Biolabs, Beverly MA, USA) and 2 μL of 10 μM standard Illumina P1 and P2 primers ...
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bioRxiv - Bioengineering 2020Quote: ... PCR was performed using Q5® High-Fidelity 2× Master Mix (New England Biolabs, US). The point mutation in dCas9 to create H840A Cas9n was made using Q5® Site-Directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Bioengineering 2020Quote: ... or colony PCR using Q5® High-Fidelity 2× Master Mix (New England Biolabs, US) if a chromosomal region was targeted ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was then resuspended in 100 μl/sample volume of 1X NEBuffer 2 (NEB) supplemented with Triton X-100 to 0.1% and transferred to a new tube ...
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bioRxiv - Cancer Biology 2021Quote: ... were incubated at 37 °C for 2 hours in 1x T4 DNA ligase buffer (NEB). Following this incubation 1200 units T4 DNA ligase (NEB ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA (2 μg) was digested with the AluI restriction enzyme (New England Biolabs, U.S.A) and purified by using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... and 2 μL T7 RNA Polymerase mix (HiScribe T7 High Yield RNA Synthesis Kit, NEB) were incubated at 37°C for 3 h ...
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Cellular and structural basis of synthesis of the unique intermediate dehydro-F420-0 in mycobacteriabioRxiv - Biochemistry 2020Quote: ... For Southern blotting analysis 2 μg of gDNA was digested with appropriate restriction enzymes (NEB) at 37 °C for 16 hours ...
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bioRxiv - Neuroscience 2020Quote: ... 1μL DNase I and 2 μL 10x DNase buffer (New England Biolabs, Ipswich, MA, USA), and incubated for 30 min at 37°C ...