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Citations for New England Biolabs :
1601 - 1650 of 9500 citations for QuantiChrom Iron Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... or homologous recombination based NEBuilder® HiFi DNA Assembly kit (NEB, MA). Site-directed mutagenesis was carried out using QuikChange QuikChange Site-Directed Mutagenesis Kit (Agilent ...
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bioRxiv - Plant Biology 2024Quote: ... Using NEBNext Ultra II kits for Illumina (New England BioLabs, Ipswich, MA), each pool was indexed with a unique NEBNext i7 adapter and an i5 adapter containing a degenerate barcode (for removal of PCR duplicates ...
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bioRxiv - Synthetic Biology 2024Quote: ... The HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs) was used for in vitro transcription according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs). Gel fragments were extracted using GeneJet Gel extraction kit (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: Plasmid libraries are generated using Golden Gate Assembly Kits (NEB, BsaI-HFv2). The reactions undergo a cycling protocol of 30 alternating 5-minute 37°C and 16°C cycles followed by a final 60°C 5-minute hold ...
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bioRxiv - Immunology 2024Quote: ... PCR and Gibson Assembly® Cloning Kit (New England Biolabs, Ipswich, MA). The product was transformed in E ...
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bioRxiv - Genomics 2024Quote: ... using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA). These libraries were then sequenced to produce 150-bp paired-end reads using a NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Luna Universal One-Step RT-qPCR kit (New England Biolabs) with the following primers:
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bioRxiv - Microbiology 2024Quote: ... The reaction system was prepared according to the kit’s requirements (Yugong Biolabs) and fluorescence quantitative analysis was performed using the ABI 7500 PCR system (Applied Biosystems) ...
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bioRxiv - Zoology 2024Quote: ... The Monarch® RNA purification kit (New England Biolabs, Whitby, ON, Canada) was used to purify synthesized dsRNA following the manufacturer’s recommendation ...
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bioRxiv - Physiology 2024Quote: ... cDNA was synthesized using a cDNA Reverse Transcriptase Kit (New England Biolabs). TaqMan-based real time quantitative polymerase chain reactions (qRT-PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... products were purified with the Monarch PCR DNA cleanup kit (NEB, Inc.) and analyzed by endpoint PCR ...
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bioRxiv - Microbiology 2023Quote: ... purification (Monarch PCR & DNA Cleanup Kit, New England Biolabs Cat. No. T1030), and ligation with T4 DNA ligase (New England Biolabs Cat ...
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bioRxiv - Biochemistry 2024Quote: ... Point mutations were generated using the Q5 Site-Directed Mutagenesis Kit (NEB). Primer sequences and plasmid construct information is listed in Supplementary Table 1.
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II kit (NEB E7645) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs E5520S). The fusion construct was then amplified by PCR and inserted into the multiple-cloning site of the lentiviral vector pLEX using the same NEBuilder cloning kit to generate pLEX-mNFL-GFP ...
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bioRxiv - Microbiology 2024Quote: ... they were purified using Monarch DNA Gel Extraction kit (New England Biolabs) and assembled by two-step PCR (supplementary methods ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNA was isolated using a Monarch Total RNA Miniprep Kit (NEB), RNA quantity and quality were determined with a NanoDrop OneC Spectrophotometer (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2024Quote: dsRNA was generated by in vitro transcription (NEB T7 E2050s transcription kit) from PCR products generated by the following forward and reverse primers (separated by comma):
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bioRxiv - Biochemistry 2023Quote: ... PRIMPOL S255D mutant was generated using Q5 site-directed mutagenesis kit (NEB). The pCS2 NLS-mCherry-PRIMPOL WT or S255D vectors were used for transfection in U2OS PRIMPOL KO cells using TransIT (Mirus ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using the ProtoScript First Stand cDNA Synthesis Kit (NEB). The obtained cDNA was diluted 10-fold to 200 μL with nuclease-free water ...
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bioRxiv - Cell Biology 2024Quote: ... The NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, USA) was used for generating the RNA-Seq library for each sample ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using NEBNext small RNA sequencing kit (New England Biolabs) or NextFlex small RNA sequencing kit (Perkin Elmer ...
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bioRxiv - Molecular Biology 2023Quote: ... Inserts were acquired by PCR using KOD Hotstart Mastermix from NovaGen and purified from gel using Monarch DNA Gel Extraction kit from NEB. All cloning was performed in E ...
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bioRxiv - Synthetic Biology 2023Quote: ... cDNA was synthesized using a LunaScript® RT SuperMix Kit (NEB E3010), and real time PCR was run on a Bio-Rad CFX96 Touch Real-Time PCR Detection System using an iTaq Universal SYBR Green Supermix (BioRad #1725121) ...
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bioRxiv - Developmental Biology 2024Quote: ... IVT sgRNA products were purified Monarch PCR and DNA Cleanup Kit (NEB) and quantified ...
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bioRxiv - Plant Biology 2023Quote: ... mRNA was purified with the NEB mRNA isolation kit (New England Biolabs). Extracted RNA was treated with DNase I (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... We used a magnetic mRNA isolation kit (New England BioLabs, Ipswich, MA) to enrich samples for mRNA before proceeding to library preparation ...
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bioRxiv - Developmental Biology 2024Quote: ... was mutated using Q5 site directed mutagenesis kit (NEB, primers: SP (RSV) AP2 (hTFAP2A)-CRISPR RES-fw and SP (RSV ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by the Monarch™ Total RNA Miniprep Kit (New England BioLabs) applying the manufacturer’s protocol for TRIzol® extracted samples ...
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bioRxiv - Genomics 2024Quote: ... The DNA was extracted with NEB Monarch gel purification kit (NEB, T1020S) using two columns and eluted in 30 µL of the elution buffer.
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Recurrent loss of crossover interference punctuates the recombination landscape across yeast speciesbioRxiv - Genomics 2024Quote: DNA extraction was performed using the Monarch HWW DNA extraction kit (NEB) following the manufacturers protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... which were then inserted using NEBuilder® HiFi DNA Assembly kit (NEB). The expression cassette for WT or mutant RAD6 in the pRS41H backbone was excised with Kpn1 and Sac1 and inserted into the same sites in vector pRS305 (LEU2 ...
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bioRxiv - Biochemistry 2024Quote: ... All mutangenesis were performed by Q5 Site-Directed Mutagenesis Kit (NEB; E0554S). For pCAG-OSF-FOXA1 (N-terminus 2xStrep-Flag-tagged ...
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bioRxiv - Biochemistry 2024Quote: ... using Q5® Site-Directed Mutagenesis Kit (New England BioLabs, cat # E0554S) with the following primers.
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bioRxiv - Bioengineering 2024Quote: ... gDNA extraction using Monarch® Genomic DNA Purification Kit (New England Biolabs), and histology sectioning.
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bioRxiv - Bioengineering 2024Quote: ... gDNA extraction using Monarch® Genomic DNA Purification Kit (New England Biolabs), and histology sectioning.
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bioRxiv - Biochemistry 2024Quote: ... and ligated together with NEBuilder HiFi DNA Assembly Kit (New England Biolabs). Escherichia coli DH5α competent cells were transformed with the ligation mixtures and the cells containing assembled plasmid were selected on an agar medium supplemented with 100 mg/l ampicillin ...
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bioRxiv - Cell Biology 2024Quote: ... followed by NEBNext Ultra II Direction Prep Kit for Illumina (NEB Cat #). Library sizes was measured using Bioanalyzer and pooled libraries concentration was calculated using NEBNext Lib Quant Kit (NEB Cat#) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR was performed using the Q5 High-Fidelity DNA Polymerase Kit (NEB) and primers specific for the microsatellite expansion adapters (1X Q5 Reaction Buffer ...
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bioRxiv - Microbiology 2023Quote: ... AvcI was then purified using Monarch® RNA Cleanup Kit (NEB™). Purity of the product was evaluated using a 1.0% TBE agarose gel ...
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bioRxiv - Genetics 2023Quote: ... and PCR products were then purified by PCR & DNA Cleanup Kit (NEB). STR PCR products were ligated with the pClone007 simple vector (Tsingke) ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesised using LunaScript™ RT SuperMix Kit (New England Biolabs) before analysis via qRT-PCR ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were isolated from selected transformants using Monarch plasmid miniprep kit (NEB) and subjected to sequencing reactions (Eurofins ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA cleavage products were purified using Monarch RNA Cleanup kit (NEB) and divided into two parts ...
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bioRxiv - Synthetic Biology 2023Quote: ... they were assembled employing the NEBuilder® HiFi DNA Assembly kit (NEB). The assembled plasmids were structured as follows ...
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bioRxiv - Developmental Biology 2023Quote: ... libraries were prepared with an NEBnext DNA Library Prep kit (NEB, E7370L). Adaptor-ligated DNA was size-selected and purified with AMPure XP beads (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified using Monarch PCR/DNA Cleanup Kit (New England BioLabs). ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs ...
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bioRxiv - Synthetic Biology 2023Quote: ... Libraries were assembled using the HiFi DNA Assembly kit (NEB, cat #E2621) from degenerate UltramerTM oligos (IDT) ...
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bioRxiv - Biochemistry 2023Quote: RT-PCR was completed using Taq DNA polymerase (Taq PCR kit, NEB), according to the manufacturer’s protocol ...