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Citations for New England Biolabs :
1601 - 1650 of 3658 citations for Diethyl 2 5 diaminoterephthalate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Dangling ends were removed by a 5 min incubation with Exonuclease III (NEB #0206) at 37 °C and biotin enrichment was done using 20 ul Dynabeads™ MyOne™ Streptavidin C1 beads (Invitrogen #65001) ...
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bioRxiv - Biochemistry 2023Quote: ... activation was performed overnight at 4 °C with 5 μg of Factor Xa (NEB) per mg of protein ...
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bioRxiv - Cancer Biology 2023Quote: ... A volume of 40 μl reaction mix containing 5 μl isothermal amplification buffer (NEB), 3 μl 100 mM MgSO4 ...
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bioRxiv - Genetics 2023Quote: ... 5% DMSO and 1× NEBNext High-Fidelity PCR master mix (New England BioLabs, M0541L) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Injection mixtures contained 10μl restriction digest including 5 units of I-SceI enzyme (NEB), 1μl CutSmart buffer ...
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bioRxiv - Biochemistry 2023Quote: ... 5 µg of genomic DNA from the clones were digested solely with XhoI (NEB) overnight at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... cleaned and concentrated 20x using the Monarch PCR & DNA Cleanup Kit (5 µg) (NEB). Further cleanup was done with the E-gel imager system (Invitrogen ...
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bioRxiv - Evolutionary Biology 2023Quote: ... which was purified directly over Monarch DNA Cleanup Columns (5 μg) (New England Biolabs) using a 10:1 ratio of binding buffer (a modified version of Qiagen’s PB buffer ...
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bioRxiv - Genomics 2024Quote: ... RNAs were 5’dephosporylated through 90 minutes incubation in total with thermostable QuickCIP (NEB) in which the samples were briefly heated to 75°C and quickly chilled on ice at the 60 minutes mark ...
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bioRxiv - Molecular Biology 2024Quote: ... and then 3’-end dephosphorylated and 5’-end phosphorylated using T4 polynucleotide kinase (NEB). tRNA library preparation used SuperScript™ IV following the QuantM-tRNA-seq protocol (Pinkard et al ...
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bioRxiv - Genetics 2024Quote: ... along with an additional 5 µL 2x Gibson Assembly Master Mix (NEB Cat#E2611L). The reaction mixture was incubated at 50°C for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 uL of 5 x mRNA Second Strand Synthesis buffer (New England Biolabs, E6111L), and 0.25 uL of mRNA Second Strand Synthesis enzyme (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... with 5 μL Quick-Load pBR322 DNA-MspI Molecular Marker (New England Biolabs Inc.) in a separate lane ...
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bioRxiv - Biochemistry 2024Quote: ... The assembly reaction was transformed into NEB 5-alpha competent cells (New England Biolabs). After the recovery step ...
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bioRxiv - Molecular Biology 2024Quote: ... Coverslips were then washed twice for 5 min in 1x rCutSmart buffer (NEB; #B6004S) and once in 1x blunting buffer (NEB ...
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bioRxiv - Genetics 2024Quote: ... Dangling ends were removed by a 5 min incubation with Exonuclease III (NEB #0206) at 37 °C and biotin enrichment was done using 20 ul DynabeadsTM MyOneTM Streptavidin C1 beads (Invitrogen #65001) ...
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bioRxiv - Microbiology 2024Quote: 5’ RACE was done using a Template Switching (TS) Reverse Transcriptase Enzyme mix (NEB) that takes advantage of a template switching reverse transcriptase and a template switching oligonucleotide (TSO) ...
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bioRxiv - Molecular Biology 2024Quote: ... coli 5-alpha and purified using the Monarch Plasmid Miniprep Kit (New England Biolabs).
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bioRxiv - Molecular Biology 2024Quote: ... and fragment inserts were cloned into digested pLS21-5 using Gibson assembly (NEB, E5510S). The plasmid pARL01 ...
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bioRxiv - Neuroscience 2024Quote: ... Each PCR reaction was subsequently supplemented with 5 μL of CutSmart Buffer (NEB B7204) and digested using 1μL DpnI restriction enzyme (NEB R0176 ...
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bioRxiv - Genomics 2024Quote: ... we added 5 pg of unmethylated DNA from phage λ (New England Biolabs, E7123A) per 10 ng sample DNA ...
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bioRxiv - Plant Biology 2024Quote: ... for 30 minutes and the supernatant mixed with 5 mL Chitin Resin (NEB #S6651), previously washed first with 30 mL of ddH2O and then 20 mL of HEGX buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 5-10 μg of plasmid was linearised with the restriction enzyme NotI (NEB, R3189L). The linearised plasmids were run on a gel to ensure complete digestion ...
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bioRxiv - Synthetic Biology 2024Quote: ... Transformations used the NEB recommended protocols for chemical transformation (NEB 5-alpha, Cat# C2987H) or electroporation (NEB 10-beta ...
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bioRxiv - Bioengineering 2024Quote: ... digested vec-tors were treated with 5 units of Antarctic Phosphatase (New England Biolabs) for 30 min at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... The purified plasmid DNA (5 µg) was incubated with T4 DNA ligase (NEB, M0202) (2.5 µL ...
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bioRxiv - Synthetic Biology 2024Quote: ... Constructed plasmids were transformed and maintained in NEB 5-alpha (New England Biolabs (NEB) C2987H ...
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bioRxiv - Synthetic Biology 2024Quote: ... Constructed plasmids were transformed and maintained in NEB 5-alpha (New England Biolabs (NEB) C2987H ...
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bioRxiv - Genetics 2024Quote: ... 5 μL NEB T4 DNA Ligase from the NEB Quick Ligation Module (NEB, E6056), and 1.0 μL Native Adapter (NA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then immediately added 5 µL of a solution with 1X Ligase Buffer (NEB), 0.75 mM ATP (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... RNase H treatment on fixed cells was performed using 5 U RNase H (M0297, NEB) for 3 h at 37°C ...
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bioRxiv - Developmental Biology 2021Quote: ... with subsequent end repair/dA-tailing reaction using Klenow Fragment (3’-5’ exo-) (NEB M0212S) and ligation with Illumina sequencing adapters using T4 DNA ligase (NEB) ...
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bioRxiv - Developmental Biology 2020Quote: 5-7 μg of HiC library in a total volume of 100 μl (1x NEB buffer 2.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... a set of 5’-end biotinylated anti-sense DNA oligoes and 5ul RNase inhibitor (NEB) were added to the lysate ...
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bioRxiv - Molecular Biology 2020Quote: ... oligonucleotides were 5-end labeled with 32P-γATP using T4 polynucleotide kinase (New England Biolabs). Supercoiled pUC19 plasmid dsDNA was prepared by a method that did not involve DNA denaturation (Clewell and Helinski ...
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bioRxiv - Genomics 2020Quote: ... A-tailing 3’ end was performed using Klenow Fragment (3’→5’ exo-) (New England Biolabs), and then TruSeq Adapters were ligated by Quick T4 DNA Ligase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: 5’ RACE was performed using the Template Switching RT Enzyme Mix (New England Biolabs M0466) according to manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: Decapping of 5 µg total RNA was performed with 200 units of yDcpS (NEB M0463S) in 10mM Bis-Tris-HCl pH 6.5 ...
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bioRxiv - Genomics 2021Quote: ... The ligation mix (5 μL of LNB, 3 μL of Quick ligase (New England Biolabs), 1.2 μL of MQ ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by addition of 3’-A overhangs using Klenow Fragment 3’-5’ exo- (NEB, M0212S). After denaturation of DNA at 95°C for 3 min ...
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bioRxiv - Biochemistry 2021Quote: ... RNA substrates were 5’ end labeled using γ32P-ATP and T4 Polynucleotide Kinase (NEB, MA)38 ...
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bioRxiv - Pathology 2021Quote: ... The second strand was synthesized with Klenow Fragments (3′-5′ exo-; New England Biolabs Inc.) and complement chains of NSR primers ...
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bioRxiv - Cell Biology 2021Quote: ... purified HEK-proGATI (∼5 μg) was denatured and deglycosylated using deglycosylation mix II (NEB, #P6044S), which contains the enzymes needed to remove N-linked and many common O-linked glycans ...
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bioRxiv - Evolutionary Biology 2020Quote: ... samples were digested for 15 min at 37 °C with 5 μL of ExoI (NEB) to remove first round primers ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μg standard glycoprotein was mixed in 1 μL of 10× glycoprotein denaturing buffer (NEB) (final conc ...
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bioRxiv - Biochemistry 2021Quote: RNA substrates (listed in Table S1) were either 5’ labelled by T4 polynucleotide kinase (NEB) and 5’ 32P-γ −ATP ...
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bioRxiv - Biochemistry 2020Quote: ... 5:1 molar ratio of insert to backbone and 1X Gibson Assembly Master Mix (NEB) and incubated at 50°C for 1 hour before transformation into One Shot TOP10 Chemically Competent E ...
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bioRxiv - Biochemistry 2020Quote: ... 5:1 molar ratio of insert to backbone and 1X Gibson Assembly Master Mix (NEB) and incubated at 50°C for 1 hour before transformation into One Shot TOP10 Chemically Competent E ...
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bioRxiv - Biochemistry 2020Quote: ... The 5’ phosphate of transcribed RNA ligands was removed using calf intestinal phosphatase (CIP, NEB) and then replaced with 32P using T4 polynucleotide kinase (PNK ...
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bioRxiv - Microbiology 2020Quote: ... T5 exonuclease diluted 1:5 with 5X reaction buffer (New England BioLabs; 0.01 units/μL), Taq DNA ligase (New England BioLabs ...