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Citations for New England Biolabs :
1601 - 1650 of 5887 citations for 7 Diethylamino 3 nitro 2H 1 benzopyran 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 15 μl 20% SDS and 3 μl 20 mg/ml Proteinase K (NEB #P8107) was added to the sample and the mixture was incubated at 37 °C for 30 min with agitation at 1400 rpm to completely lyze the cells ...
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bioRxiv - Biochemistry 2022Quote: ... and then incubating with either Klenow Fragment (3’→5’ exo-) (New England Biolabs (NEB), M0212L ...
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bioRxiv - Biochemistry 2022Quote: ... and then incubating with either Klenow Fragment (3’→5’ exo-) (New England Biolabs (NEB), M0212L ...
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bioRxiv - Developmental Biology 2021Quote: ... custom NTP mix was prepared with 3’-O-Me-m7G cap analogue (60mM, NEB), GTP (75mM ...
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bioRxiv - Immunology 2020Quote: ... and 3’ end A-overhangs were added by NEBNext® dA-Tailing Module (NEB). The A-tailed products were subsequently cloned into pGEM®-T Easy vector (Promega ...
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bioRxiv - Microbiology 2019Quote: ... the 3’ adapter-ligated DNA fragments were adenylated using 5’ DNA Adenylation Kit (NEB) in a 20-μl reaction following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1.5 μl Klenow Fragment (3’->5’ exo-) (NEB, cat#M0212S, 5 U/μl) per amplicon pool reaction ...
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bioRxiv - Synthetic Biology 2020Quote: ... This was done in 50 μl reactions using 3 μl dual index primers (NEB), 135 ng purified PCR product from the previous reaction and 25 μl Q5 high fidelity master mix (NEB) ...
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bioRxiv - Developmental Biology 2020Quote: ... act57B sequence: 5’-UCUUCCCCUC-3’ RNA oligonucleotides were end-labeled using T4 Kinase (NEB) with ATP [γ-32P]32 ...
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bioRxiv - Physiology 2020Quote: ... 636 bp 3’ arm of homology) with the nicking enzyme Nb.Bsm1 (New England Biolabs) and purification from an alkaline-agarose gel ...
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bioRxiv - Molecular Biology 2022Quote: ... and/or 10 U of T4 PNK (wild-type or 3’ phosphatase minus, NEB). For RtcB ligation ...
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bioRxiv - Biochemistry 2022Quote: ... 3’A overhang were added to each fragment using the Klenow fragment (NEB #M0212M) and DNA was purified using MinElute PCR Purification Kit (QIAGEN #28006) ...
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bioRxiv - Genomics 2022Quote: ... the end-repaired DNA was mixed with Klenow Fragment (3′ → 5′ exo−) (NEB, #E6044A) in NEBNext dA-Tailing Reaction Buffer (NEB ...
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bioRxiv - Genomics 2022Quote: ... Illumina sequencing adapters containing uracil hairpin loop structure and 3′ T overhangs (NEB, #E7337A) were ligated to the DNA by adding 150 units of Quick T4 DNA Ligase (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 3’UTR mutant was obtained by using the Site Directed Mutagenesis (New England Biolabs). miR-19a ...
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bioRxiv - Immunology 2022Quote: ... To digest remaining M1 tag forward oligo: 3 µl 20U/ µl Exonuclease I (NEB) was added and samples were incubated for 1 h at 37°C ExoI denaturation was performed for 5 min at 98°C ...
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bioRxiv - Cell Biology 2023Quote: ... and Exon 3 amplified using Q5® High-Fidelity DNA Polymerase (New England Biolabs). The following primers were used ...
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bioRxiv - Molecular Biology 2023Quote: ... and then 3’-end dephosphorylated and 5’-end phosphorylated using T4 polynucleotide kinase (NEB). tRNA library preparation used SuperScript™ IV following the QuantM-tRNA-seq protocol (Pinkard et al ...
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bioRxiv - Molecular Biology 2023Quote: ... free adapters were removed by adding 3 units of RecJf (New England Biolabs, M0264) and 1.7 units of 5ʹ deadenylase (NEB ...
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bioRxiv - Microbiology 2023Quote: ... then barcoded RNA adapters were ligated to the 3′ end (T4 RNA Ligase, NEB). Samples were separated by SDS-PAGE and transferred to nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2024Quote: ... HR template was divided into 3 parts and each part was amplified by NEB Q5 polymerase using AJ51 & AJ52 oligonucleotides for part 1 ...
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bioRxiv - Biochemistry 2024Quote: ... crosslinked RNA fragments were dephosphorylated at their 3’-ends using T4-PNK (NEB, M0201S) and ligated to a pre-adenylated 3’-adapter (L3-App ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg RNA were incubated for 30 minutes at 37°C with 2 μl RNA 5’ Pyrophosphohydrolase (NEB, M0356, 5,000 units/ml). The reactions were stopped by cleaning up the samples following the ZYMO RNA Clean & Concentrator-5 kit (ZYMO ...
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bioRxiv - Microbiology 2022Quote: ... RNA was subjected to OneStep qRT-PCR analysis using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, Frankfurt am Main, Germany) and a CFX96 Real-Time System ...
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bioRxiv - Microbiology 2023Quote: ... and the expression levels of genes were quantified with the Luna® universal one-step RT-qPCR kit (New England Biolabs, Ipswich, MA, USA) with the following cycling parameters ...
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bioRxiv - Systems Biology 2023Quote: ... Purified DNA fragments were assembled by One Step Cloning Kit (Vazyme Co., Ltd. Cat. C113) or Golden gate assembly54(NEB, Cat. M0202 and R3733). Sequence contents of genetic parts are collected in Supplementary Table 11.
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bioRxiv - Evolutionary Biology 2021Quote: ... 2 uL of T4 RNA ligase I (NEB M0204S) totaling 20 uL and incubated for 1 hour at 30 C.
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bioRxiv - Molecular Biology 2021Quote: ... 2 μl 10X RtcB reaction buffer (New England Biolabs), 2 μl 1mM GTP ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by adding 2 μl of RNase A (NEB) and incubating at 37 °C for 45 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 1x T4 RNA Ligase 2 reaction buffer (NEB, M0239S), and 0.5 units/µl T4 RNA Ligase 2 (NEB ...
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bioRxiv - Genomics 2019Quote: ... 5 μl of NEB Buffer 2 (New England Biolabs), 2 μl dNTP mix (2.5 mM) ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 ng of 2-log DNA ladder (NEB N3200) was included for reference ...
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bioRxiv - Genomics 2020Quote: ... the NEBNext High-Fidelity 2× PCR Master Mix (NEB) was used according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in 5 ml 1x NEB Buffer 2 (NEB) and drop frozen in liquid nitrogen ...
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bioRxiv - Genomics 2021Quote: ... 25 μL NEBNext HiFi 2× PCR Master mix (NEB) was added ...
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bioRxiv - Bioengineering 2021Quote: ... 2 unit of murine RNase inhibitor (New England Biolabs), varying amounts of target RNA ...
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bioRxiv - Bioengineering 2021Quote: ... Its constituents were 1X RNA Ligase 2 buffer (NEB) supplemented with 5% PEG 8000 ...
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bioRxiv - Biochemistry 2020Quote: ... followed by incubation with 2 Units USER enzyme (NEB) for 10 min at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... packed with 2 ml amylose resin (New England Biolabs) equilibrated with buffer AF ...
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bioRxiv - Genomics 2022Quote: ... 0.7 µl T4 ligase (2 M U/ml, NEB), 250 ng DNA (adapters in File S1 ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 units of phi29 DNA polymerase (New England Biolabs), three not to quantified deoxynucleoside triphosphate mix (20 μM) ...
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bioRxiv - Microbiology 2022Quote: ... 20 μl Phusion DNA polymerase (2 U/μl, NEB), 160 μl Taq DNA ligase (40 U/μl ...
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bioRxiv - Genomics 2022Quote: ... 2 μl of undiluted Thermolabile Proteinase K (NEB #P8111S) and 1 μl SUPERaseIN (20 U/μl ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μl of 10 mM dNTPs (New England Biolabs), 2.5 μl of 10 μM oligonucleotide A (unmodified forward primer ...
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bioRxiv - Cancer Biology 2019Quote: ... 10 µl Deglycosylation Mix Buffer 2 (New England Biolabs) was added to 17 µg of protein a 40 µl total volume ...
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bioRxiv - Biochemistry 2020Quote: ... followed by incubation with 2 Units USER enzyme (NEB) for 10 min at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... for 2 hours or ShortCut RNaseIII (New England Biolabs) for 20 minutes at 37°C after permeabilization before blocking.
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl Thermostable 5’App ligase (New England Biolabs), 4 μl 10 μM pre-adenylated R1R Adapter ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.5 vol 2×Phusion Master Mix (New England Biolabs), and 0.04 vol P1 and P2 amplification primers (10 nm) ...
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bioRxiv - Microbiology 2019Quote: ... and ExoI (New England Biolabs, 2 U/µg RNA) for 30 min at 37°C ...