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Citations for New England Biolabs :
1551 - 1600 of 9451 citations for Human Zyxin ZYX ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... or fragment insertion using the Gibson assembly Cloning kit (New England Biolabs) were employed according to the supplier’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Mutagenesis of complement templates was performed using the Q5 Mutagenesis kit (NEB) with primers referenced in the primer table S1.
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bioRxiv - Microbiology 2022Quote: ... reverse transcribed and amplified with OneTaq one-step RT-PCR kit (NEB) for verification by Sanger sequencing.
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bioRxiv - Molecular Biology 2022Quote: ... The guide RNAs (gRNAs) were generated by EnGen sgRNA Synthesis Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... mRNA was isolated using the Magnetic mRNA Isolation Kit (New England Biolabs), followed by rRNA depletion using the RiboMinus Eukaryote System v2 (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed according to NEBNext Small RNA Library Prep kit (NEB) protocol ...
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bioRxiv - Microbiology 2023Quote: ... All other plasmids were constructed using a HiFi DNA assembly kit (NEB). All plasmids were confirmed by Sanger sequencing (Genewiz) ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting RNA was purified using the RNA cleanup kit (#T2040, NEB) and injected into 1-2 cell stage gcna/acrc maternal mutant embryos or into control WT embryos (1nl of the mRNA solution ...
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bioRxiv - Systems Biology 2022Quote: ... and 2μL of NEBridge Golden Gate Assembly Kit (BsmBI-v2) (NEB #E1602L), added in that order ...
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bioRxiv - Molecular Biology 2022Quote: ... were generated using a Q5 site directed mutagenesis kit (New England Biolabs) according to the manufacturer’s instructions and expressed and purified as for VCB.
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bioRxiv - Molecular Biology 2022Quote: ... and purified with the Monarch Plasmid Miniprep Kit (T1010, New England Biolabs). Plasmids were digested with restriction enzymes to generate linearized templates for in vitro transcription ...
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bioRxiv - Microbiology 2022Quote: ... or Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) or LightCycler® Multiplex RNA Virus Master (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... and Monarch Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA, U.S.A.) protocol ...
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bioRxiv - Cell Biology 2022Quote: ... or homologous recombination based NEBuilder® HiFi DNA Assembly kit (NEB, MA). Site-directed mutagenesis was carried out using QuikChange QuikChange Site-Directed Mutagenesis Kit (Agilent ...
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bioRxiv - Plant Biology 2024Quote: ... Using NEBNext Ultra II kits for Illumina (New England BioLabs, Ipswich, MA), each pool was indexed with a unique NEBNext i7 adapter and an i5 adapter containing a degenerate barcode (for removal of PCR duplicates ...
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bioRxiv - Synthetic Biology 2024Quote: ... The HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs) was used for in vitro transcription according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs). Gel fragments were extracted using GeneJet Gel extraction kit (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: Plasmid libraries are generated using Golden Gate Assembly Kits (NEB, BsaI-HFv2). The reactions undergo a cycling protocol of 30 alternating 5-minute 37°C and 16°C cycles followed by a final 60°C 5-minute hold ...
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bioRxiv - Immunology 2024Quote: ... PCR and Gibson Assembly® Cloning Kit (New England Biolabs, Ipswich, MA). The product was transformed in E ...
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bioRxiv - Genomics 2024Quote: ... using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, USA). These libraries were then sequenced to produce 150-bp paired-end reads using a NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Luna Universal One-Step RT-qPCR kit (New England Biolabs) with the following primers:
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bioRxiv - Microbiology 2024Quote: ... The reaction system was prepared according to the kit’s requirements (Yugong Biolabs) and fluorescence quantitative analysis was performed using the ABI 7500 PCR system (Applied Biosystems) ...
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bioRxiv - Zoology 2024Quote: ... The Monarch® RNA purification kit (New England Biolabs, Whitby, ON, Canada) was used to purify synthesized dsRNA following the manufacturer’s recommendation ...
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bioRxiv - Physiology 2024Quote: ... cDNA was synthesized using a cDNA Reverse Transcriptase Kit (New England Biolabs). TaqMan-based real time quantitative polymerase chain reactions (qRT-PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... products were purified with the Monarch PCR DNA cleanup kit (NEB, Inc.) and analyzed by endpoint PCR ...
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bioRxiv - Microbiology 2023Quote: ... purification (Monarch PCR & DNA Cleanup Kit, New England Biolabs Cat. No. T1030), and ligation with T4 DNA ligase (New England Biolabs Cat ...
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bioRxiv - Biochemistry 2024Quote: ... Point mutations were generated using the Q5 Site-Directed Mutagenesis Kit (NEB). Primer sequences and plasmid construct information is listed in Supplementary Table 1.
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II kit (NEB E7645) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs E5520S). The fusion construct was then amplified by PCR and inserted into the multiple-cloning site of the lentiviral vector pLEX using the same NEBuilder cloning kit to generate pLEX-mNFL-GFP ...
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bioRxiv - Microbiology 2024Quote: ... they were purified using Monarch DNA Gel Extraction kit (New England Biolabs) and assembled by two-step PCR (supplementary methods ...
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bioRxiv - Microbiology 2024Quote: In vitro transcription/translation assays were conducted using the PURExpress kit (NEB) according to the manufacturer’s protocol with a 2-hour incubation at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNA was isolated using a Monarch Total RNA Miniprep Kit (NEB), RNA quantity and quality were determined with a NanoDrop OneC Spectrophotometer (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2024Quote: dsRNA was generated by in vitro transcription (NEB T7 E2050s transcription kit) from PCR products generated by the following forward and reverse primers (separated by comma):
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bioRxiv - Biochemistry 2023Quote: ... PRIMPOL S255D mutant was generated using Q5 site-directed mutagenesis kit (NEB). The pCS2 NLS-mCherry-PRIMPOL WT or S255D vectors were used for transfection in U2OS PRIMPOL KO cells using TransIT (Mirus ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using the ProtoScript First Stand cDNA Synthesis Kit (NEB). The obtained cDNA was diluted 10-fold to 200 μL with nuclease-free water ...
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bioRxiv - Cell Biology 2024Quote: ... The NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, USA) was used for generating the RNA-Seq library for each sample ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using NEBNext small RNA sequencing kit (New England Biolabs) or NextFlex small RNA sequencing kit (Perkin Elmer ...
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bioRxiv - Molecular Biology 2023Quote: ... Inserts were acquired by PCR using KOD Hotstart Mastermix from NovaGen and purified from gel using Monarch DNA Gel Extraction kit from NEB. All cloning was performed in E ...
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bioRxiv - Synthetic Biology 2023Quote: ... cDNA was synthesized using a LunaScript® RT SuperMix Kit (NEB E3010), and real time PCR was run on a Bio-Rad CFX96 Touch Real-Time PCR Detection System using an iTaq Universal SYBR Green Supermix (BioRad #1725121) ...
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bioRxiv - Developmental Biology 2024Quote: ... IVT sgRNA products were purified Monarch PCR and DNA Cleanup Kit (NEB) and quantified ...
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bioRxiv - Plant Biology 2023Quote: ... mRNA was purified with the NEB mRNA isolation kit (New England Biolabs). Extracted RNA was treated with DNase I (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... We used a magnetic mRNA isolation kit (New England BioLabs, Ipswich, MA) to enrich samples for mRNA before proceeding to library preparation ...
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bioRxiv - Developmental Biology 2024Quote: ... was mutated using Q5 site directed mutagenesis kit (NEB, primers: SP (RSV) AP2 (hTFAP2A)-CRISPR RES-fw and SP (RSV ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by the Monarch™ Total RNA Miniprep Kit (New England BioLabs) applying the manufacturer’s protocol for TRIzol® extracted samples ...
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bioRxiv - Genomics 2024Quote: ... The DNA was extracted with NEB Monarch gel purification kit (NEB, T1020S) using two columns and eluted in 30 µL of the elution buffer.
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Recurrent loss of crossover interference punctuates the recombination landscape across yeast speciesbioRxiv - Genomics 2024Quote: DNA extraction was performed using the Monarch HWW DNA extraction kit (NEB) following the manufacturers protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... which were then inserted using NEBuilder® HiFi DNA Assembly kit (NEB). The expression cassette for WT or mutant RAD6 in the pRS41H backbone was excised with Kpn1 and Sac1 and inserted into the same sites in vector pRS305 (LEU2 ...
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bioRxiv - Biochemistry 2024Quote: ... All mutangenesis were performed by Q5 Site-Directed Mutagenesis Kit (NEB; E0554S). For pCAG-OSF-FOXA1 (N-terminus 2xStrep-Flag-tagged ...
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bioRxiv - Biochemistry 2024Quote: ... using Q5® Site-Directed Mutagenesis Kit (New England BioLabs, cat # E0554S) with the following primers.
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bioRxiv - Bioengineering 2024Quote: ... gDNA extraction using Monarch® Genomic DNA Purification Kit (New England Biolabs), and histology sectioning.