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Citations for New England Biolabs :
1551 - 1600 of 9461 citations for Human Malonyl CoA Decarboxylase Mitochondrial MLYCD ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Adenylated 3’ adapter was prepared using the 5’ DNA adenylation kit (NEB) and ligated using T4 RNA ligas’ 2 ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was extracted with the Monarch Total RNA miniprep kit T2010 (NEB), 1ug of which was converted to cDNA by a RT-PCR reaction using the High Capacity RNA-to-cDNA kit (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2022Quote: ... mRNA synthesis reactions were cleaned using Monarch RNA Cleanup Kit (NEB: #T2040L) and visualized using agarose gel-electrophoresis ...
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bioRxiv - Cell Biology 2022Quote: ... The PCR products were transcribed with T7 HiScribe kit (New England Biolabs) and transfected together with TrueCas9 protein v2 (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... Point mutations were made using the Q5 Site-Directed Mutagenesis Kit (NEB) with mutagenic primers or mutagenic double-stranded DNA fragments (gBlocks™ ...
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bioRxiv - Cell Biology 2022Quote: ... and sgRNAs were purified with Monarch RNA Cleanup Kit (New England Biolabs). sgRNA sequences and PCR primers used in this study are listed in Supplementary Table 2.
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bioRxiv - Genomics 2022Quote: RNA extraction was performed using the Monarch Total RNA Miniprep Kit (NEB) according to the manufacturer’s protocol.
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bioRxiv - Genetics 2022Quote: ... polyadenylated with the E.coli Poly (A) polymerase Kit (NEB, cat. no. M0276L), and purified with the RNeasy Mini Kit (QIAGEN ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR products were cleaned (Monarch PCR & DNA Cleanup Kit, NEB cat. T1030S) and Sanger-sequenced ...
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bioRxiv - Microbiology 2022Quote: ... Products were PCR cleaned up with Monarch PCR & DNA cleanup kit (NEB), ligated into pCR2.1-TOPO vector (Thermo fisher ...
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bioRxiv - Microbiology 2022Quote: ... or fragment insertion using the Gibson assembly Cloning kit (New England Biolabs) were employed according to the supplier’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Mutagenesis of complement templates was performed using the Q5 Mutagenesis kit (NEB) with primers referenced in the primer table S1.
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bioRxiv - Microbiology 2022Quote: ... reverse transcribed and amplified with OneTaq one-step RT-PCR kit (NEB) for verification by Sanger sequencing.
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bioRxiv - Molecular Biology 2022Quote: ... The guide RNAs (gRNAs) were generated by EnGen sgRNA Synthesis Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... mRNA was isolated using the Magnetic mRNA Isolation Kit (New England Biolabs), followed by rRNA depletion using the RiboMinus Eukaryote System v2 (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were constructed according to NEBNext Small RNA Library Prep kit (NEB) protocol ...
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bioRxiv - Microbiology 2023Quote: ... All other plasmids were constructed using a HiFi DNA assembly kit (NEB). All plasmids were confirmed by Sanger sequencing (Genewiz) ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting RNA was purified using the RNA cleanup kit (#T2040, NEB) and injected into 1-2 cell stage gcna/acrc maternal mutant embryos or into control WT embryos (1nl of the mRNA solution ...
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bioRxiv - Systems Biology 2022Quote: ... and 2μL of NEBridge Golden Gate Assembly Kit (BsmBI-v2) (NEB #E1602L), added in that order ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized using the ProtoScript First Stand cDNA Synthesis Kit (NEB). The obtained cDNA was diluted 10-fold to 200 μL with nuclease-free water ...
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bioRxiv - Molecular Biology 2022Quote: ... were generated using a Q5 site directed mutagenesis kit (New England Biolabs) according to the manufacturer’s instructions and expressed and purified as for VCB.
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bioRxiv - Molecular Biology 2022Quote: ... and purified with the Monarch Plasmid Miniprep Kit (T1010, New England Biolabs). Plasmids were digested with restriction enzymes to generate linearized templates for in vitro transcription ...
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bioRxiv - Microbiology 2022Quote: ... or Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) or LightCycler® Multiplex RNA Virus Master (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... and Monarch Total RNA Miniprep Kit (New England Biolabs, Ipswich, MA, U.S.A.) protocol ...
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bioRxiv - Cell Biology 2022Quote: ... or homologous recombination based NEBuilder® HiFi DNA Assembly kit (NEB, MA). Site-directed mutagenesis was carried out using QuikChange QuikChange Site-Directed Mutagenesis Kit (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNA was isolated using a Monarch Total RNA Miniprep Kit (NEB), RNA quantity and quality were determined with a NanoDrop OneC Spectrophotometer (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2024Quote: ... IVT sgRNA products were purified Monarch PCR and DNA Cleanup Kit (NEB) and quantified ...
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bioRxiv - Biochemistry 2024Quote: ... and ligated together with NEBuilder HiFi DNA Assembly Kit (New England Biolabs). Escherichia coli DH5α competent cells were transformed with the ligation mixtures and the cells containing assembled plasmid were selected on an agar medium supplemented with 100 mg/l ampicillin ...
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bioRxiv - Biochemistry 2024Quote: ... All mutangenesis were performed by Q5 Site-Directed Mutagenesis Kit (NEB; E0554S). For pCAG-OSF-FOXA1 (N-terminus 2xStrep-Flag-tagged ...
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bioRxiv - Bioengineering 2024Quote: ... gDNA extraction using Monarch® Genomic DNA Purification Kit (New England Biolabs), and histology sectioning.
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bioRxiv - Bioengineering 2024Quote: ... gDNA extraction using Monarch® Genomic DNA Purification Kit (New England Biolabs), and histology sectioning.
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bioRxiv - Developmental Biology 2024Quote: ... was mutated using Q5 site directed mutagenesis kit (NEB, primers: SP (RSV) AP2 (hTFAP2A)-CRISPR RES-fw and SP (RSV ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by the Monarch™ Total RNA Miniprep Kit (New England BioLabs) applying the manufacturer’s protocol for TRIzol® extracted samples ...
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bioRxiv - Molecular Biology 2023Quote: ... which were then inserted using NEBuilder® HiFi DNA Assembly kit (NEB). The expression cassette for WT or mutant RAD6 in the pRS41H backbone was excised with Kpn1 and Sac1 and inserted into the same sites in vector pRS305 (LEU2 ...
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bioRxiv - Biochemistry 2024Quote: ... using Q5® Site-Directed Mutagenesis Kit (New England BioLabs, cat # E0554S) with the following primers.
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bioRxiv - Cancer Biology 2024Quote: ... PCR was performed using the Q5 High-Fidelity DNA Polymerase Kit (NEB) and primers specific for the microsatellite expansion adapters (1X Q5 Reaction Buffer ...
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bioRxiv - Cell Biology 2024Quote: ... followed by NEBNext Ultra II Direction Prep Kit for Illumina (NEB Cat #). Library sizes was measured using Bioanalyzer and pooled libraries concentration was calculated using NEBNext Lib Quant Kit (NEB Cat#) ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II kit (NEB E7645) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... The HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs) was used for in vitro transcription according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Using NEBNext Ultra II kits for Illumina (New England BioLabs, Ipswich, MA), each pool was indexed with a unique NEBNext i7 adapter and an i5 adapter containing a degenerate barcode (for removal of PCR duplicates ...
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bioRxiv - Microbiology 2024Quote: ... they were purified using Monarch DNA Gel Extraction kit (New England Biolabs) and assembled by two-step PCR (supplementary methods ...
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bioRxiv - Neuroscience 2024Quote: ... using the NEBuilder HiFi DNA Assembly cloning kit (New England Biolabs E5520S). The fusion construct was then amplified by PCR and inserted into the multiple-cloning site of the lentiviral vector pLEX using the same NEBuilder cloning kit to generate pLEX-mNFL-GFP ...
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bioRxiv - Genomics 2024Quote: ... The DNA was extracted with NEB Monarch gel purification kit (NEB, T1020S) using two columns and eluted in 30 µL of the elution buffer.
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Recurrent loss of crossover interference punctuates the recombination landscape across yeast speciesbioRxiv - Genomics 2024Quote: DNA extraction was performed using the Monarch HWW DNA extraction kit (NEB) following the manufacturers protocol ...
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bioRxiv - Cell Biology 2024Quote: ... The NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB, USA) was used for generating the RNA-Seq library for each sample ...
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bioRxiv - Microbiology 2024Quote: In vitro transcription/translation assays were conducted using the PURExpress kit (NEB) according to the manufacturer’s protocol with a 2-hour incubation at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... We used a magnetic mRNA isolation kit (New England BioLabs, Ipswich, MA) to enrich samples for mRNA before proceeding to library preparation ...
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bioRxiv - Synthetic Biology 2023Quote: ... cDNA was synthesized using a LunaScript® RT SuperMix Kit (NEB E3010), and real time PCR was run on a Bio-Rad CFX96 Touch Real-Time PCR Detection System using an iTaq Universal SYBR Green Supermix (BioRad #1725121) ...
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bioRxiv - Molecular Biology 2023Quote: ... Inserts were acquired by PCR using KOD Hotstart Mastermix from NovaGen and purified from gel using Monarch DNA Gel Extraction kit from NEB. All cloning was performed in E ...
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bioRxiv - Plant Biology 2023Quote: ... mRNA was purified with the NEB mRNA isolation kit (New England Biolabs). Extracted RNA was treated with DNase I (Thermo Fisher ...