Labshake search
Citations for New England Biolabs :
1551 - 1600 of 3340 citations for 7 Decyn 1 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... by adding 1 µl (20 units) of BamHI restriction enzyme (New England Biolabs) to 1 µg DNA ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were treated with 1 μM SNAP-cell 647-SiR (New England Biolabs) for 30 minutes ...
-
bioRxiv - Genomics 2021Quote: ... 20% PEG8000 and 20 U T4 RNA Ligase 1 (New England Biolabs, M0204) at 16°C for 12 hours.
-
bioRxiv - Genomics 2020Quote: ... and 1 μL of Phi29 (Catalog number: M0269L Vendor: New England Biolabs Inc). The combined products are cleaned at a 0.5x bead ratio and eluted in 10 μl of nuclease-free water.
-
bioRxiv - Biophysics 2020Quote: ... 1 µl of Universal nuclease (Pierce; 125U) and 10 of DNaseI (NEB; 20U) were added to the sample and incubate for 10 min at RT ...
-
bioRxiv - Genomics 2020Quote: ... The reaction mix included 1 μl of 10x poly(A) polymerase buffer (NEB), 0.1 mM of ATP ...
-
bioRxiv - Molecular Biology 2021Quote: ... The digested nuclei were ligated with 1 μl of T4 DNA ligase (NEB) in 100 μl of 1 × T4 ligation buffer for 24 h at 16°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNA 5’ adaptor was ligated using T4 RNA Ligase 1 - high concentration (NEB) and 10 mM ATP ...
-
bioRxiv - Immunology 2020Quote: ... 5µl Quick-Load Purple 1 kb DNA ladder (New England Biolabs, Ipswich, Massachusetts) was used as reference ...
-
Glucocorticoid receptor collaborates with pioneer factors and AP-1 to execute genome-wide regulationbioRxiv - Genomics 2021Quote: ... 1.35 μL MNase (New England Biolabs, diluted 1:10 in MNase reaction buffer) was added to the resuspended chromatin and incubated at 37°C ...
-
bioRxiv - Genetics 2020Quote: ... we prepared PCRs in final 25 μl volumes consisting of 1× buffer (NEB Hot Start Taq® 2X Master Mix ...
-
bioRxiv - Microbiology 2022Quote: ... save for a 1 hour incubation with 0.3μL (3U) of DpnI (NEB R0176) at 37°C instead of using PspGI ...
-
bioRxiv - Biochemistry 2022Quote: ... 1 uM (in ntds; ~100 pM molecules) bacteriophage lambda DNA (New England Biolabs), 25 nM fSSB (tetramer ...
-
bioRxiv - Developmental Biology 2022Quote: ... 1 mM ATP with 60U of High Concentration T4 RNA ligase (NEB, M0437M) at 16°C overnight ...
-
bioRxiv - Cancer Biology 2022Quote: ... and NEBNext Multiplex Oligos for Illumina (NEB, Set 1 #E7335S, Set 2 #E7500S). ChIP libraries were done following NEB’s guidelines (NEB ...
-
bioRxiv - Genomics 2022Quote: ... Samples were treated with 1 µl of Exonuclease I (New England Biolabs, M0293S) and subsequently pooled for SPRI bead cleanup ...
-
bioRxiv - Microbiology 2022Quote: ... After the ligation of adaptors using T4 RNA ligase 1 (New England Biolabs), reverse transcription was executed ...
-
bioRxiv - Synthetic Biology 2022Quote: ... PCR-1 was carried out using 2X Taq polymerase mastermix (New England BioLabs) while PCR-2 and PCR-3 were carried out using dATP ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 μg of total RNA was treated with amplification-grade DNase I (NEB) and reverse transcribed using Oligo(dT)20 as a primer and Superscript III Reverse Transcriptase (Life Technologies ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNA was circularized using T4 RNA ligase 1 (Epicentre or New England BioLabs) and reverse transcribed using Superscript III (Invitrogen ...
-
bioRxiv - Genetics 2022Quote: ... was digested for 1 h with 50 units of DpnI (New England Biolabs) to eliminate unreplicated plasmids ...
-
bioRxiv - Neuroscience 2023Quote: ... for 1 hour at 37 °C and SfiI (New England Biolabs, catalog #R0123) for 5 hours at 50 °C ...
-
bioRxiv - Microbiology 2023Quote: ... 1 unit of Phusion Polymerase were mixed with 1x HF Phusion buffer (NEB) and made up to 50 µl total volume with H20 ...
-
bioRxiv - Plant Biology 2022Quote: ... Of each entry plasmid 100 ng was combined with 1× Cutsmart buffer (NEB), 1 mM ATP ...
-
bioRxiv - Systems Biology 2023Quote: ... NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB EE7600S), and an Illumina NextSeq 550 75-cycle high-output kit (Illumina #20024906) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... and 1 µl of a dual unique index multiplex primer pair (NEB; E6440) in a total reaction volume of 25 µl ...
-
bioRxiv - Synthetic Biology 2022Quote: ... into backbones previously digested with corresponding restriction enzymes (NEB, 1 h 37° C) to yield intermediate plasmids containing individual transcriptional units ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 µL was used in a 10 µL Kinase/Ligase/DpnI (KLD, NEB) reaction and incubated at room temperature for 10-20 min ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 μg BSA per well dealt as negative control (NEB, Ipswich, MA, USA). Samples were applied in a final volume of 100 μl coating buffer (100 mM Tris-HCL pH 8 ...
-
bioRxiv - Systems Biology 2024Quote: ... and 1 µl of Klenow large fragment DNA polymerase (New England Biolabs M0210) were then added ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 ul of 10x Template Switching Reverse Transcriptase (New England Biolabs, MA, USA) for the synthesis of the 1st strand ...
-
bioRxiv - Molecular Biology 2024Quote: ... Adaptor ligation was performed with a 1:10 dilution of adaptor (NEB, E6440S). For DNA purification ...
-
bioRxiv - Neuroscience 2024Quote: ... the construct was diluted 1:5 with empty pUC19 vector (New England Biolabs) and then transfected with 7.5 µL of TransIT-293 and 2.5 µg of DNA ...
-
bioRxiv - Cell Biology 2024Quote: ... and/or 1 µM SiR-647-SNAP-dye (New England Biolabs, Waltham, MA) or 100 nM DAPI (Life Technologies) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Nuclei were taken up in 520 µl of 1× DpnII buffer (NEB, R0543M) and incubated with 7.5 µl of 20% SDS for 1 hour shaking at 600 r.p.m ...
-
bioRxiv - Genetics 2024Quote: ... for 1 hour and then treated with 1.6 units of Proteinase K (NEB) for a further hour before cleanup of DNA with AMPure XP Beads (Beckman Coulter ...
-
bioRxiv - Microbiology 2023Quote: ... and 1 μl T4 RNA ligase 2 (truncated K227Q, NEB, 200,000 units/ml) and incubated at 16°C for 14 h ...
-
bioRxiv - Cancer Biology 2023Quote: ... NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) oligos (NEB E7600S) was used for assigning indices to all samples prior to pooling ...
-
bioRxiv - Immunology 2023Quote: All PD-1 mutants were generated using Q5 site-directed mutagenesis kit (NEB) following the manufacture’s protocol ...
-
bioRxiv - Genomics 2023Quote: ... and Uracil-Specific Excision Reagent (1 U/μl USER, catalog num. M5505L, NEB) treatment ...
-
bioRxiv - Microbiology 2023Quote: 1 µg of total RNA was treated with DNase-I (New England Biolabs) followed by cDNA synthesis using iScript cDNA synthesis kit (Bio-rad ...
-
bioRxiv - Molecular Biology 2023Quote: ... the extracted cDNA was then added to 1 x Terminal Transferase buffer (NEB), 250 µM CoCl2 (NEB) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The clarified lysate is mixed with 1 mL of amylose resin (E8021S, NEB) pre-equilibrated with lysis buffer and incubated in the cold room using an end-over-end rotor for 2 hours ...
-
bioRxiv - Immunology 2023Quote: ... The 3lll adaptor was added using T4 RNA ligase 1 (New England Biolabs) for 2.5 hours at 37°C ...
-
bioRxiv - Biochemistry 2023Quote: ... The final Golden Gate reaction volume contained 1× concentrated T4 ligase buffer (NEB), 250 ng Golden Gate vector (pcDNA3-based carrying a twin Strep-FLAG tag ...
-
bioRxiv - Genetics 2023Quote: ... the rRNA was eliminated by adding 1 μl RNase H (New England Biolabs), 4 μl 10X RNase H buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... 0.5% Triton X-100) supplemented with Proteinase K (1:100, New England Biolabs). Specimens were incubated on an orbital shaker at 37°C per 2 hours and 60 rpm.
-
bioRxiv - Biophysics 2023Quote: ... The left lane (“L”) has a DNA size ladder (1 kb+, NE Biolabs). The black arrow in the left lane (“P” ...
-
bioRxiv - Cell Biology 2023Quote: ... RNA was first circularized by a T4 RNA Ligase 1 Kit (M0204. NEB) and then purified by TRIzol reagent followed by isopropanol precipitation ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1) by NEB following the manufacturer’s protocol ...