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Citations for New England Biolabs :
1501 - 1550 of 10000+ citations for Glutathione Fluorescent Detection Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 5 mM DTT) prior to incubating with 0.5 μCi/μl [γ32P]ATP and T4 PNK (NEB, M0201L) to radiolabel crosslinked RNA ...
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bioRxiv - Molecular Biology 2022Quote: miRNAs were synthesized by IDT and radio-labeled at the 5’ end with T4 polynucleotide kinase (NEB) and (γ-32P ...
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bioRxiv - Microbiology 2022Quote: ... followed by adding a 5’ cap using the Vaccina Capping System (cat#M2080S, New England Biolabs Inc.) and a poly-A tail using E.coli Poly(A ...
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bioRxiv - Microbiology 2022Quote: ... 5 ng of α-HA ChIP or input DNA were end-repaired with T4 DNA polymerase (NEB), Klenow DNA polymerase (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... 45 nM pegRNA3-3AGTGA (Appendix Table S1) and 5 µl NEBuilder HiFi DNA Assembly Master Mix (NEB) and incubated at 37°C for 1 h.
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bioRxiv - Molecular Biology 2022Quote: Approximately 5 μg genomic DNA were digested with 1.5 μl restriction enzymes + 2.5 μl CutSmart buffer (NEB) for 5 hrs at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... which was dephosphorylated at the 5’-end with 3 µl of Quick-CIP (5000 U/µl, NEB) in a total volume of 20 µl according to manufactureŕs instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... After CIP inactivation (2 min at 80°C) piRNAs were 5΄end radiolabeled by T4 PNK (NEB) with [γ-32P] ATP (10mCi/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was 5’-end-labeled with 32P using T4 polynucleotide kinase (New England Biolabs catalogue number M0201L). Samples were separated using 4–12% BisTris-PAGE and transferred to a nitrocellulose membrane (Protran) ...
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bioRxiv - Cell Biology 2022Quote: ... 5 μg of DNA was digested with HinfI and RsaI restriction enzymes (New England BioLabs, 50U each) at 37 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′Iq Competent Escherichia coli (High Efficiency) (NEB, Cat# C2992H), and isolated with PureYield Plasmid Miniprep System (Promega ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription reactions (RT +) were then performed with 5 µg of RNA and random hexamers (NEB, #S1330S), using the Superscript III Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... pETduet-1::5′-flank_SpecPromoter_kanR_3′-flank and were subsequently digested from the plasmid using BamHI and NotI (NEB). The resultant insert (5′-flank_SpecPromoter_kanR_3′-flank ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 12·5 μL of Q5 Hot Start High-Fidelity 2X Master Mix (New England BioLabs, M0494S), 1·1 μL of 10 μM SARS-Cov2-Midnight-1200 primer (either Pool 1 or Pool 2)(17 ...
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bioRxiv - Genetics 2023Quote: ... and amplified for 5 cycles using NEBNext High-Fidelity 2X PCR Master Mix (New England Biolabs; M0541L). After evaluation by real-time PCR ...
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bioRxiv - Microbiology 2023Quote: ... CVO689-CVO586 (integrant 5’ end) and CVO321-CVO183 (integrant 3’ end) using Q5 Polymerase (New England Biolabs). PCRs were set up according to manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were treated with JET (12.5 nM d-o-p) and/or ScaI-HF (NEB, 5 U) for 15 minutes at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... VCE or FCE::T7RNAP fusion and 5 U/μL vaccinia cap 2′-O-methyltransferase (New England Biolabs). Reactions were carried out at indicated temperatures for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were centrifuged (500xg, 5 min, 4°C) and washed once with 1X NEBuffer 2.1 (NEB, #B7202). For nucleosome depletion ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of PCR product from the inner primer amplification was cleaned using 5μL Exonuclease I (NEB) and 2μL rSAP (NEB) ...
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bioRxiv - Physiology 2024Quote: ... Glycan digestion was achieved with the following enzyme concentrations: 5 μL of α2-3,6,8,9 neuraminidase A (20 U/μL, New England Biolab, NEB) with 1x GlycoBuffer 1 (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... then combined with 5 μM dNTPs and 2 μL of 10× rCutSmart buffer (NEB, Ipswich, MA, USA) in a total reaction volume of 17 μL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Nuclei were pelleted at 500 ξ g at 4°C for 5 minutes and resuspended in 0.5 mL of 1.2x NEBuffer r2.1 (New England Biolabs) containing 3% sodium dodecyl sulfate (SDS ...
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bioRxiv - Neuroscience 2024Quote: ... 50 µg of protein lysate was treated with 5 µL NeuA (a2-3,6,8,9 Neuraminidase A, P0722L, NEB) or 5 µL of O-Glycosidase (P0733L ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The assembled plasmid was transformed into NEB 5-alpha (high efficiency) competent cells (New England Biolabs; C2987). After verifying the sequence ...
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bioRxiv - Bioengineering 2024Quote: ... Initial studies were done in an alternate NEB® 5-alpha strain (New England Biolabs, Catalog # C2987H) where trpA and trpB were replaced with a chloramphenicol resistance cassette ...
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bioRxiv - Molecular Biology 2024Quote: ... fragments were dephosphorylated by adding 5 μL antarctic phosphatase buffer and 2 μL enzyme (NEB cat#M0289S) and incubating for 1 h at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then purified using the DNA cleanup column kit (the Monarch PCR & DNA cleanup kit from NEB).
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bioRxiv - Biochemistry 2020Quote: ... Samples were then purified using the DNA cleanup column kit (the Monarch PCR & DNA cleanup kit from NEB). The specificity of AlkB repair reaction was confirmed using an inactive AlkB control reaction in which Fe2+ ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Genetics 2022Quote: ... or FLAG (amino acid sequence: DYKDDDDK) with a kit (Gibson assembly kit from New England Biolabs, catalogue # E5510S). The different combinations were cloned into pJFRC7-20XUAS-IVS-mCD8::GFP (Addgene # 26220 ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was performed using the NEBNext Ultra DNA Library Prep Kit (kit number E7370L, New England Biolabs) and 8 cycles of PCR ...
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bioRxiv - Microbiology 2023Quote: ... DNA was isolated from blood (QiaAmp DNA Mini Blood Kit, Qiagen; or Monarch Genomic DNA purification kit, NEB) or from perfused organs homogenized (Fisher Bead Mill 4 ...
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bioRxiv - Developmental Biology 2023Quote: ... NEB One Taq RT-PCR kit (One Taq® RT-PCR Kit, New England Biolabs INC, Frankfurt, Germany) was used for cDNA synthesis ...
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bioRxiv - Synthetic Biology 2021Quote: ... or Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2021Quote: NEBNext rRNA Depletion Kit (NEB, Cat E6310S) was used to deplete ribosomal RNA according to the manufacture’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... The HiScribe Quick T7 kit (NEB, E2050S) was then used for in vitro transcription ...
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bioRxiv - Molecular Biology 2020Quote: ... a quick ligation kit (New England Biolabs) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... Q5 Site-Directed Mutagenesis Kit (NEB E0554S) and restriction cloning were used to generate partial constructs ...
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bioRxiv - Synthetic Biology 2019Quote: ... Q5 site directed mutagenesis kit from NEB was used for cloning the inserts inferiors at 100 bps ...
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bioRxiv - Microbiology 2019Quote: ... and end repaired (Quick Blunting Kit, NEB). Transposon junctions were amplified from gDNA by PCR ...
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bioRxiv - Developmental Biology 2019Quote: ... and Monarch Total RNA miniprep kit (NEB). RNA concentration was measured using Nanodrop (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Phusion high fidelity DNA polymerase kit (NEB) was used for the PCR amplification and optimized with DMSO and formamide ...
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bioRxiv - Neuroscience 2019Quote: The Q5 Site-Directed Mutagenesis Kit (NEB) was used to create malt-1(C374A ...
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bioRxiv - Cell Biology 2019Quote: ... annealed and ligated (Quick Ligase kit, NEB) into BbsI (NEB ...
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bioRxiv - Genomics 2019Quote: ... blunt-end repair (Quick Blunting kit, NEB), 3’-adenylation (Klenow Fragment 3’ to 5’ exo- ...
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bioRxiv - Bioengineering 2019Quote: ... using the Gibson Assembly Cloning Kit (NEB), producing the plasmids pgRNAMPN372-142 and pgRNAMPN372-400 respectively ...
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bioRxiv - Bioengineering 2019Quote: ... using the Gibson Assembly Cloning Kit (NEB). The resulting plasmid pgRNA-triple-target is transformed in E ...
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bioRxiv - Physiology 2021Quote: ... and Monarch PCR Cleanup Kit (NEB, T1030). PCR products were electrophoresed in 2% agarose gel (TopVision Agarose Tablets ThermoFisher R2801 ...
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bioRxiv - Cancer Biology 2020Quote: ... using the Gibson Assembly kit (NEB#E2611). To verify the identity and relative representation of sgRNAs in the pooled plasmids ...