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Citations for New England Biolabs :
1451 - 1500 of 10000+ citations for Estrone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: NEBNext FFPE DNA repair kit (NEB M6630S)
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bioRxiv - Genomics 2023Quote: Monarch Genomic DNA Purification Kit (NEB T3010S)
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bioRxiv - Genetics 2023Quote: ... or the Monarch HMW DNA kit (NEB) according to manufacturer’s instructions ...
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Chimeric Antigen Cytotoxic Receptors for In-Vivo Engineering of Tumor-targeting Natural Killer CellsbioRxiv - Immunology 2023Quote: ... and Monarch® RNA purification kit (NEB) was used to purify the final mRNA according to the manufacturer’s protocol.
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bioRxiv - Synthetic Biology 2024Quote: ... The NEBuilder HiFi DNA Assembly kit (NEB) was used to create the plasmid EPG-IRES-GCaMP6m ...
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bioRxiv - Molecular Biology 2024Quote: ... NEBNext Small RNA Library Prep kit (NEB), RNA oligonucleotides (Horizon Discoveries) ...
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bioRxiv - Cell Biology 2024Quote: ... Monarch® DNA Gel Extraction Kits (NEB) were used per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... an NEB Q5 SDM kit (NEB, E0554S) was used ...
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bioRxiv - Genomics 2024Quote: ... Q5 Site-directed mutagenesis kit (#E0554S, NEB) was used to produce intronless ...
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bioRxiv - Microbiology 2023Quote: ... using Quick Ligase kit (New England Biolabs). Ligation was used to transform E ...
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bioRxiv - Evolutionary Biology 2021Quote: DNA libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit kit by New England Biolabs (NEB) and the NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 ...
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bioRxiv - Genomics 2020Quote: NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext rRNA Depletion Kit (NEB, Ipswich, MA) was used to generate RNA-seq libraries from purified RNA following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: Libraries were constructed using NEBNext Ultra RNA Library Prep for Illumina kits (New England Biolabs kits E7490 and E7530), as per manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... a ChIP-seq library was made using the NEBNext Ultra II DNA Library Prep Kit for Illumina kit (NEB).
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bioRxiv - Cell Biology 2023Quote: The novel entry modules created for this study were cloned by insertion of PCR-amplified sequences in pJet1.3 or pMiniT 2.0 following manufacturer’s instructions (CloneJET PCR Cloning Kit, Thermo Scientific; NEB® PCR Cloning Kit, NEB). BsaI recognition sites followed by module-specific overhangs were added to each primer used to amplify entry sequences ...
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bioRxiv - Physiology 2023Quote: ... including DNase 1 treatment (Monarch Total RNA Miniprep kit, #T2010, New England Biolabs, USA and QIAGEN RNeasyMini kit, #74104). Purity and quantity of RNA were assessed by determining the optical density (OD ...
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bioRxiv - Genomics 2023Quote: ... Final immunoprecipitated DNA samples from the kit were then purified using a Monarch PCR and DNA cleanup kit (5μg) (NEB), with a final elution volume of 50μL in TE buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... libraries were quantified by qPCR using a library quantification kit (NEBNext® Library Quantification Kit, E7630, New England Biolabs). Illumina sequencing was performed on a HiSeq2500 instrument for 100 single end read cycles (Genome Sequencing Core ...
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bioRxiv - Microbiology 2020Quote: ... Following KLD (kinase, ligase, and DpnI) treatment and transformation into NEB 5-alpha F’Iq cells (New England Biolabs), cells were grown as near-lawns on LB-chloramphenicol plates and a miniprep was performed from a resuspension of ~5000 colonies for each library.
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bioRxiv - Molecular Biology 2019Quote: ... 5 μL of Standard Taq Reaction Buffer (Final concentration 2.5×, 3.75 mM MgCl2, New England BioLabs, MA, USA), 1 μL of dNTPs (final concentration 0.5 mM ...
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bioRxiv - Genomics 2021Quote: ... the purified products are treated with Klenow fragment (3’ → 5’ exo-) (Cat. No. M0212L; NEB; use 1 uL) and Taq DNA polymerase (Cat ...
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bioRxiv - Biochemistry 2020Quote: ... the top strand (MB733) was 5′ labelled with ϒ[32P]- ATP and T4 polynucleotide kinase (T4 PNK; NEB) for 1 h at 37°C and cleaned up with a G-50 micro column as above ...
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bioRxiv - Developmental Biology 2021Quote: ... the synthesized sgB was injected at 300ng/µL after 5-minute incubation at RT with Cas9 protein (NEB) at 2µM final in 20mM Hepes-NaOH pH 7.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 μL (approximately 250 ng) of the extracted DNA was first digested using the restriction enzyme DpnII (NEB), diluted to 100 μL and ligated with T4 ligase (NEB) ...
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bioRxiv - Developmental Biology 2020Quote: ... 37.5 μl of 0.4 mM biotin-14-dATP and 10 μl of 5 U/ μl Klenow (NEB, M0210L) were added and mixed by pipetting ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA was then 5’ end labeled in 24μL PNK wash buffer with 16μL of 1x PNK buffer (NEB) containing 150μCi of gamma P32-ATP ...
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bioRxiv - Neuroscience 2021Quote: NFIB 3’ UTR and 5’ UTR HP forming regions were in vitro transcribed using T7 transcriptase (NEB, E2040) and purified with Trizol extraction (described in RT-qPCR paragraph) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was denatured again and pyrophosphates were removed from the 5’-end by 10 U RppH (M0356S, NEB) in 1× NEB buffer 2 (10 mM Tris-HCl pH 7.9 ...
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bioRxiv - Molecular Biology 2019Quote: ... the 5′ linker (containing four random nucleotides) was ligated to the small RNAs using T4 RNA ligase (NEB) overnight at 16°C ...
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bioRxiv - Synthetic Biology 2020Quote: ... was achieved by PCR of the relevant design using primers F_RiboJ_Rem and R_RiboJ_Rem (Supplementary Table 5) and subsequent circularization by standard Golden Gate assembly (New England Biolabs, E1601S) to create the plasmids pVB003 and pVB004 ...
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bioRxiv - Biophysics 2020Quote: ... Fluorescence labelling was done by incubating with 5 nmol SNAP-Surface Alexa Fluor 488 (#S9129S, New England Biolabs) for 1 hour at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... 5 µg of Intein-RapGAP-SIPA1L2 (470-838) and Intein-Caldendrin were immobilized on chitin resin (NE BioLabs). Resin was washed with TBS buffer (150 mM NaCl ...
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bioRxiv - Genomics 2020Quote: ... RNA was resuspended in 5 μl of water and the 3′ ends dephosphorylated with PNK (New England BioLabs) in MES buffer (100 mM MES-NaOH ...
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bioRxiv - Neuroscience 2019Quote: ... Ten microliters of PCR products were digested with Nsi1 (3 hours with 5 units of Nsi1 enzyme (NEB)) and then ...
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bioRxiv - Plant Biology 2020Quote: ... were amplified from genomic DNA using primers in Supplemental Table 5 and Taq Phusion polymerase (New England BioLabs), cloned in pGEM-t Easy (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... 15 μg of DNA-depleted RNA was incubated with 12.5 units of RNA 5’ pyrophosphohydrolase (RppH) (#M0356, NEB) in a 50 μl reaction for 1 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... the cap-1 structure was added to the 5’ end using the Vaccinia capping enzyme (New England Biolabs) and Vaccinia 2’-O-methyltransferase (New England Biolabs) ...
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bioRxiv - Genomics 2020Quote: ... Chromatin within intact nuclei was then digested for 5 minutes with micrococcal nuclease (NEB #M0247, 500U/1M cells) prior to nuclei lysis by Covaris sonication ...
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bioRxiv - Biochemistry 2021Quote: ... gDNA was quantified by Nanodrop and a fraction of DNA was treated with 5 U RNase H (NEB) for 1h at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... Residual template switch adaptor was removed by incubation with 5 U of uracil DNA glycosylase (New England BioLabs) for 40 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... and was then installed with 5’cap (Vaccinia Capping System, NEB, USA; Cap 2’-O-methyltransferase, NEB, USA) and 3’ Poly(A ...
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bioRxiv - Microbiology 2019Quote: ... 20 μg of total RNA were ligated with 50 pmol 3′adaptor Linker (5′-rAppCTGTAGGCACCATCAAT–NH2-3′; NEB) through a 16 h incubation at 16°C with 20 U T4 RNA ligase (Ambion) ...
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bioRxiv - Biochemistry 2022Quote: 3’-PUA-DNA was generated by incubation of 1 μM 5’-FAM-labeled AP-DNA (FAM_U_35) with 20 U EndoIII/Nth (NEB) in Buffer B pH 7.0 at 37 °C for 60 m ...
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bioRxiv - Microbiology 2022Quote: ... 2.5µL of 10µM MBTUni-12 primer + 2.5µL of 10µM MBTUni-13 primer (5’-ACGCGTGATCAGTAGAAACAAGG-3’) + 10µL 5x HF Phusion Buffer + 1µL 10mM dNTPs mix (NEB #N0447S) + 0.5µL Phusion Polymerase + 28.5µL of Nuclease-free water (Ambion ...
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bioRxiv - Biochemistry 2021Quote: ... 5 µL of PCR product from the inner primer amplification was cleaned used 0.5 µL Exonuclease I (NEB) and 1 µL rSAP (NEB) ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were incubated on ice for 5 minutes then immediately treated with appropriate concentration of RNAse If(NEB) for 3 mins at 37C at 1200RPM ...
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bioRxiv - Cell Biology 2021Quote: ... 5 µg antibody (Table. S3) and 50 µl protein A magnetic beads (New England Biolabs, Cat. No. S1425S) were added to the supernatant and incubate overnight at 4 degree Celsius ...
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bioRxiv - Molecular Biology 2019Quote: ... samples were then radiolabeled on bead with 50ul of the following mix: 5 ul 10X PNK buffer (NEB), 1 ul RNaseOUT ...
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bioRxiv - Bioengineering 2019Quote: ... 1.2 µg of the sheared DNA was blunted and 5’-phosphorylated using the NEBNext End Repair Module (NEB), and 3’-A-tailed with NEBNext dA-Tailing Module (NEB ...
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bioRxiv - Molecular Biology 2019Quote: ... The RNA templates were degraded by incubating reactions with 5 units of RNase H (M0297, NEW ENGLAND BIOLABS) and 1.0 µl of RNase cocktail enzyme mix (AM2286 ...