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Citations for New England Biolabs :
101 - 150 of 767 citations for Recombinant Human CD3d Molecule Delta CD3 TCR Complex His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... TCRs were then amplified by PCR (20 cycles with the Phusion from New England Biolabs, NEB) with a single primer pair binding to the constant region and the adapter linked to the TRAV/TRBV primers added during the reverse transcription ...
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bioRxiv - Cancer Biology 2020Quote: ... with Hi-Fi DNA builder (NEB). Primers used to amplify specific regions are described in (Supplementary Table 1) ...
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bioRxiv - Neuroscience 2022Quote: All anti-tau monoclonal antibodies were generated by the hybridoma approach against recombinant tau aggregates (human full-length tau) encapsulated in the ACM Polymersomes (ACM Biolabs, Singapore) and purified by size exclusion chromatography (SEC ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant Cas9 protein (NEB) and sgRNA prepared by in vitro transcription were added to the mixture and incubated at 37℃ for ∼ 4h to remove rRNA ...
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bioRxiv - Biochemistry 2021Quote: ... The parental plasmid molecules were inactivated by DpnI treatment (1 h, NEB, USA) and the crude reaction mixture was transformed to electrocompetent E ...
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bioRxiv - Biochemistry 2020Quote: ... The template plasmid molecules were inactivated by DpnI treatment (1 h, NEB, USA) and directly transformed to electrocompetent E ...
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bioRxiv - Biochemistry 2022Quote: ... 1 uM (in ntds; ~100 pM molecules) bacteriophage lambda DNA (New England Biolabs), 25 nM fSSB (tetramer ...
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VapC12 ribonuclease toxin modulates host immune response during Mycobacterium tuberculosis infectionbioRxiv - Microbiology 2023Quote: ... mRNA molecules are captured using magnetic Poly(T) beads (NEB, catalog no. E7490L) following purification ...
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bioRxiv - Molecular Biology 2021Quote: ... The digestions were stopped with ribonucleoside vanadyl complex (NEB S1402S) at a final concentration of 20 mM ...
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bioRxiv - Cell Biology 2019Quote: ... 400 μM Ribonucleoside Vanadyl Complex (VRC) (NEB, prod. number S1402), 400U per ml of buffer of RNASEOUT (Thermo ...
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bioRxiv - Neuroscience 2019Quote: ... and 10 mM vanadyl-ribonucleoside complex (S1402S, New England BioLabs) with probes at final concentration of 1 ng/μl ...
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bioRxiv - Microbiology 2021Quote: ... and 10 mM Ribonucleoside-Vanadyl Complex (NEB, Ipswich, MA, USA) on ice ...
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bioRxiv - Biochemistry 2022Quote: ... and the solubilized complex was incubated with Amylose resin (NEB) for 2 h at 4°C ...
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bioRxiv - Genomics 2022Quote: ... and ribonucleoside vanadyl complex (RVC, 10 mM, New England Biolabs) and fixed in 4% paraformaldehyde (PFA ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2 mM ribonucleoside-vanadyl complex (New England Biolabs, # S1402S)) ...
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bioRxiv - Immunology 2023Quote: ... 2 mM ribonucleoside vanadyl complex (New England Biolabs, Ipswich, MA), 0.02% RNase free bovine serum albumin (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... 2 mM Vanadyl Ribonucleoside Complex (Cat: S1402S; New England Biolabs), and 10 U/mL SUPERase•In RNase Inhibitor (Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... Exchange of V5/His to myc/His was performed using the Q5 site-directed mutagenesis kit (NEB #E0554) according to the manufacturer’s protocol resulting in pEF1-ZAP-S-myc/His and pEF1-ZAP-L-myc/His ...
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bioRxiv - Genomics 2022Quote: ... we first repaired DNA molecules with the NEBNext FFPE DNA Repair Mix (NEB M6630) and NEBNext Ultra II end repair/dA-tailing Module (NEB E7546) ...
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bioRxiv - Biophysics 2022Quote: ... A fraction of dynein molecules was labeled with SNAP-surface 649 (New England Biolabs) and purified using micro Bio-spin P30 gel filtration columns (33 ...
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bioRxiv - Immunology 2023Quote: ... via recombinant neuraminidase (NEB P0720L) as described by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... Nsp1–40S complex was then affinity-purified using amylose resin (NEB), followed by size-exclusion chromatography ...
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bioRxiv - Developmental Biology 2020Quote: ... and 10 mM ribonucleoside vanadyl complex (RVC; New England Biolabs, S1402S) for 10-20 minutes in a 37°C water bath ...
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bioRxiv - Cell Biology 2021Quote: ... 20 mM ribonucleoside vanadyl complex (New England Biolabs, Ipswich, MA, USA), 40 U/ml RNasin (Promega ...
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bioRxiv - Immunology 2021Quote: ... 20 mM ribonucleoside vanadyl complex (New England Biolabs, Ipswich, MA, USA), 40 U/ml RNasin (Promega ...
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bioRxiv - Immunology 2021Quote: ... 20 mM ribonucleoside vanadyl complex (New England Biolabs, Ipswich, MA, USA), 40 U/ml RNasin (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... Digestions were stopped with 20 mM ribonucleoside vanadyl complex (NEB: S1402S). The samples were then loaded onto 20%-50% sucrose gradients and ultracentrifuged in a SW41 Ti swinging-bucket rotor (Beckman 331362 ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 mM ribonucleoside vanadyl complex (New England Biolabs, Ipswich, MA), 10 minutes in 4% paraformaldehyde (Ted Pella ...
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bioRxiv - Genomics 2024Quote: ... in the presence of 1% VRC (Vanadyl Ribonucleoside Complex; NEB, S1402S). Coverslips were then rinsed 3 times in 70% EtOH and stored at -20C ...
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bioRxiv - Microbiology 2024Quote: ... 10 mM ribonucleoside-vanadyl complex (New England Biolabs, Beverley, MA, USA), and 1× complete EDTA-free protease inhibitor (Roche ...
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bioRxiv - Plant Biology 2022Quote: ... and PHB*-Delta-YFP cDNAs were reamplified and cloned into pCR8-GW via Gibson assembly (Invitrogen; NEB). Gateway LR reactions then shuttled each cDNA into pEARLYGATE100 (stable overexpression) ...
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bioRxiv - Immunology 2021Quote: ... or Amylose resin (MBP-tagged fragments, New England Biolabs) following the manufacturer’s protocols.
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bioRxiv - Biophysics 2022Quote: ... 2018) and a C-terminal 6x His tag and cloned into the pET21B vector by Hi-Fi DNA assembly (NEB).
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bioRxiv - Biophysics 2022Quote: ... The DNA fragment was amplified by PCR by inserting a C-terminal 6x His tag and cloned into the pETDUET-1 vector by Hi-Fi DNA assembly (NEB).
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bioRxiv - Microbiology 2021Quote: ... poly-A containing mRNA molecules were purified using poly-T oligo-attached magnetic beads (NEB) that were subsequently fragmented into shorter mRNA fragments using divalent cations under an elevated temperature (RNA fragmentation reagents kit ...
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bioRxiv - Genomics 2024Quote: ... The high-quality HMW DNA molecules were treated with Nb.BssS1 (New England BioLabs, Ipswich, MA) to generate single-strand breaks with sequence-specific motifs (GCTCTTC) ...
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bioRxiv - Biochemistry 2021Quote: ... 25 U Hi-T7 RNA polymerase (NEB #M0658), 250 nM RNaseAlert™ QC System v2 (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... This complex was co-injected with Cas9 protein (M0386M, NEB, Ipswich USA) into the yolk of 1-cell stage WT zebrafish embryos ...
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bioRxiv - Genomics 2023Quote: ... 0.5µL of 44µM crRNA:tracrRNA complex and 0.9µL 20µM EnGen SpyCas9 NLS (NEB) per electroporation were mixed and incubated at RT for 10-20 mins ...
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bioRxiv - Molecular Biology 2024Quote: ... The digestion was stopped with ribonucleoside vanadyl complex (NEB, Cat. No. S1402S) at a final concentration of 20 mM ...
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bioRxiv - Bioengineering 2022Quote: Recombinant Cas9 (New England Biolabs, USA) was used to generate a standard curve (20µM ...
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bioRxiv - Physiology 2022Quote: ... Recombinant CK2 was purchased from NEB. Recombinant TGFBR1 was purchased from Proqinase ...
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bioRxiv - Molecular Biology 2020Quote: ... MBP-tagged complexes were further purified on amylose resin (NEB). The core complex without MBP was instead further purified on Anti-Flag M2 affinity resin (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... MBP-tagged RBD was affinity purified by Amylose Resin (NEB) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Excess bead primers without RNA molecules were removed by the treatment of Exonuclease I (NEB, NEBM0293S). Then ...
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bioRxiv - Biophysics 2023Quote: ... Plasmid DNA molecules were purified using a commercially available purification kit (MonarchQR Plasmid Miniprep Kit, NEB). The purified plasmids were run on a 0.8% agarose gel supplemented with 2.5 µg/mL of chloroquine in 1xTBE buffer containing 2.5 µg/mL of chloroquine at 25 V for 15 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... and T478K of the Delta variant was spliced into the spike expression vector by HiFi DNA assembler cloning (NEB). The plasmid was prepared and verified as described above.
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM EGTA and 2 mM Vanadyl Ribonucleoside complex (VRC, New England Biolabs), washed three times with 70% ice-cold ethanol and kept at −20°C.
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bioRxiv - Bioengineering 2020Quote: ... a 10x Cas12-gRNA complex was prepared using 1 μM of LbCas12a (NEB) and 0.5 μM of Cy5-labeled gRNA ...
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bioRxiv - Cancer Biology 2020Quote: ... RNAsin was replaced with 10 mM ribonucleoside vanadyl complex (New England Biolabs S1402S) was added to the ground tissue ...