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Citations for New England Biolabs :
101 - 150 of 10000+ citations for Rat TGF beta 1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and libraries were prepared using the NEBNext rRNA Depletion Kit for Human/Mouse/Rat (NEB Cat#E6310). Adapter-ligated cDNA was amplified with 11 cycles of PCR to generate libraries ∼300 bp in length ...
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bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were resuspended in 1 mL spheroplast buffer (1.2 M sorbitol, 0.1 M potassium phosphate, 20 mM vanadyl ribonuclease complex [NEB S1402S], 20 µM beta-mercaptoethanol), and 1.2–5 µL 100T zymolyase (US Biological Z1005 ...
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bioRxiv - Microbiology 2023Quote: ... The purified ligation reaction was transformed into NEB-10-Beta competent cells (NEB C3020K) and the lawns resulting from this transformation were pooled together and inoculated into LB broth supplemented with kanamycin ...
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bioRxiv - Microbiology 2023Quote: ... All four libraries were independently electroporated into NEB 10-beta electrocompetent cells (NEB, C3020K) and plated on LB agar supplemented with 15 μg/ml gentamicin at 37°C overnight.
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bioRxiv - Genomics 2024Quote: ... Ligation products were transformed into either 5-alpha or 10-beta electrocompetent cells (NEB) and grown in liquid LB-Amp cultures ...
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bioRxiv - Genomics 2024Quote: ... 975 μL of pre-warmed 10-beta/Stable Outgrowth Medium was added (NEB #B9035) to each cuvette ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted using the rRNA depletion Kit (Human/Mouse/Rat) (New England BioLabs [NEB, USA]). Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted using the rRNA depletion Kit (Human/Mouse/Rat) (New England BioLabs [NEB, USA]). Library preparation for total RNA sequencing was performed using NEBNext® UltraTM II RNA Library Preparation Kit for Illumina (NEB ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was depleted of ribosomal RNAs using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs) before they were used for library construction using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... Removal of rRNA was carried out by use of the NEBNext rRNA Depletion Kit Human/Mouse/Rat (NEB) followed by strand-specific cDNA NGS library preparation (NEBNext Ultra II Directional RNA Library Prep Kit for Illumina ...
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bioRxiv - Microbiology 2021Quote: ... and rat (New England Biolabs, USA) that specifically depletes cytoplasmic (5S ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein amounts of OP18 and beta-actin were quantified using a primary stathmin polyclonal antibody (1:1000; Cell Signaling Technology, NEB GmbH, Frankfurt/Main, Germany) and a polyclonal beta-actin antibody (1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... in conjunction with the NEBNext® rRNA Depletion Kit for Human/Mouse/Rat (New England BioLabs, Ipswich, MA, USA) and the MICROBExpress kit (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: mRNA was enriched from 100ng DNase treated total RNA using the NEBNext rRNA depletion Kit (human, mouse, rat, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... A 140-500 ng aliquot of total RNA was rRNA depleted using NEB’s Human/Mouse/Rat RNAse-H based Depletion kit (New England BioLabs). Following rRNA removal ...
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bioRxiv - Immunology 2020Quote: ... transferred to 42°C and digested by immediate addition of 2ul of beta-agaraseI (NEB) per 300ul of melted gel and incubation at 42°C for 1h ...
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bioRxiv - Evolutionary Biology 2022Quote: ... transformations that involved the CRE library were performed using electroporation (NEB 10-beta electrocompetent E.coli), in all other cloning steps chemically competent E ...
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bioRxiv - Biochemistry 2024Quote: ... Purified products were again transformed into either NEB 10-beta (New England Biolabs, Ipswich, MA) or SIG10 (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2023Quote: ... according to manufacturer’s protocols and transforming chemically competent NEB 10-beta cells (NEB CN# C3019), where colony PCR and Sanger sequencing were again used to determine if the clone was correct.
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bioRxiv - Molecular Biology 2024Quote: ... The cosmid-I95 plasmid was amplified in a NEB 10-beta (New England Biolabs, C3019H), and the DNA was purified using a QIAfilter Plasmid Midi Kit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were analysed by an MSD Elisa assay (Pacific Biolabs) using a CEP55 antibody (Novux ...
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bioRxiv - Neuroscience 2021Quote: ... The polymerase chain reaction (PCR) was performed on a genomic rat DNA template using a Taq PCR Kit (New England Biolabs), and the subsequent PCR product was purified using a Qiagen PCR Purification Kit (Life Technologies ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of total RNA was depleted of rRNA using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) prior to cDNA synthesis primed with random hexanucleotide oligonucleotides (Random Primer 6, NEB). Sequencing library construction was carried out using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Genomics 2021Quote: ... or by ribodepletion with NEBNext Human/Mouse/Rat rRNA Depletion kit followed by library construction using the NEBNext Ultra II Directional RNA-Seq protocol (New England BioLabs). Illumina 2×151 paired end reads were generated either on the HiSeq 4000 or NovaSeq 6000 sequencing platforms (Illumina) ...
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bioRxiv - Genomics 2021Quote: ... 500ng input RNA was treated using the NEBNext Human/Mouse/Rat rRNA Depletion kit and libraries were prepared following the NEBNext Ultra II Directional RNA-Seq protocol (New England BioLabs). Paired end 2×151 bp reads were produced using the HiSeq 4000 platform (Illumina) ...
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bioRxiv - Cancer Biology 2022Quote: ... rRNA depletion was performed using a NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) and RNA was purified using Agencourt RNAClean XP Beads (New England Biolabs). Libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2022Quote: ... and the ligation mix was directly used to transform 10-beta competent Escherichia coli cells (NEB), to amplify pSG01 ...
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bioRxiv - Molecular Biology 2024Quote: Escherichia coli strains used in this study were NEB 10-beta competent cells (New England Biolabs, Ipswich ...
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bioRxiv - Systems Biology 2020Quote: ... rRNA depletion and library preparation for sequencing was completed with the NEBNext protocol (‘Protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760 ...
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bioRxiv - Genetics 2020Quote: ... RNA-seq libraries were prepared according to the protocol for use with NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6310) and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) ...
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bioRxiv - Immunology 2023Quote: ... Ribosomal RNA from 1000 ng total extracted RNA was depleted using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat; New England Biolabs Inc.). The remaining RNA was used to produce the sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Inc. ...
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bioRxiv - Neuroscience 2021Quote: The rat HA-tagged PRG-1 ORF construct was generated by Gibson Assembly Cloning from NEB (Ipswich, MA, #E5510S) of the Plppr4 ORF clone (NM_001001508.2 ...
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bioRxiv - Genomics 2022Quote: ... The mixture electroporated into 100 μL of 10-beta E.coli (NEB, C3020K, 2kV, 200 ohm, 25 μF) in order to achieve a transformation efficiency equal to 300-times the number of unique oligos sequences (target CFU ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Commercial competent cells were used for the K-12 DH10B strain (NEB® 10-beta high efficiency). Transformation of B REL606 and B REL607 was achieved via the chemical competence method ...
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bioRxiv - Microbiology 2019Quote: 2–3 μL of recovered DNA was electroporated into NEB® 10-beta Electrocompetent cells (C320K, NEB) and plated on chloramphenicol LB plates ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting plasmid products were transformed into NEB 10-beta electrocompetent cells (New England Biolabs, Ipswich, MA) and purified using a ZymoPURE Midiprep Kit (Zymo Research ...
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bioRxiv - Biochemistry 2024Quote: ... Nicking mutagenesis plasmid products were transformed into NEB 10-beta electrocompetent cells (New England Biolabs, Ipswich, MA) and purified using a ZymoPURE Midiprep Kit (Zymo Research ...
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bioRxiv - Molecular Biology 2021Quote: DNAse-treated RNA with high RIN value was used to deplete ribosomal RNA using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB #E6350) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... ribosomal RNA depletion was carried on the extracted RNA using Nebnext rRNA depletion kit (Human/mouse/rat) (New England BioLabs. In, USA). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... RNA-seq libraries were prepared from RNA samples (150ng) using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB Cat# E7405) in conjunction with NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB Cat# E7765) ...
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bioRxiv - Biochemistry 2023Quote: ... An mRNA transcript library for Illumina sequencing was created using the NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (New England Biolabs, Ipswich, MA). A NextSeq 500 sequencer (Illumina ...
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bioRxiv - Immunology 2023Quote: ... The other pCMVtag2B vectors containing different truncated versions of rat ITCH (Fig 2F) were generated via Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Cat#E0554S) by circularizing the PCR products amplified with the primers listed in Table S2 ...
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bioRxiv - Genomics 2023Quote: ... The recombination products were then electroporated into electrocompetent cells (NEB 10-beta, New England BioLabs, cat. No. C3020K) and plated onto Carbenicillin plates ...
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bioRxiv - Systems Biology 2023Quote: ... The concentrated ligated products were then transformed into NEB 10-beta Electrocompetent E.coli (New England Biolabs, Ipswitch, MA). Three transformations were performed to increase the yield ...
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted using phenol-chloroform and subjected to ribosomal RNA removal using a NEBNext® rRNA Depletion Kit (Human/Mouse/Rat) (NEB, Ipswich, MA). A RNAseq library was prepared by using a NEBNext® Ultra directional RNA library prep kit (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... Bands were then extracted and DNA was purified from agarose blocks using beta-Agarase I (New England BioLabs, M0392L) following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2019Quote: ... Cloning was done in bacterial strain Escherichia coli NEB® 10-beta (New England Biolabs, Frankfurt a. M., Germany) grown at 37 °C in lysogeny broth [47] supplemented with 60 mg L-1 ampicillin (Applichem ...
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bioRxiv - Cancer Biology 2023Quote: ... Bands were then extracted and DNA was isolated from agarose blocks using beta-Agarase I (New England BioLabs, M0392L) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The mixed RNA was depleted of rRNA using the Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) and fragmented using NEBNext® Magnesium RNA Fragmentation Module (NEB, cat. no. E6150S) for 5 min ...
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bioRxiv - Immunology 2020Quote: ... and on the Sprague Dawley (SD) strain (rats) (Hera Biolabs) were obtained from vendor and breed in the Division of Laboratory Animal Resources (DLAR ...