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Citations for New England Biolabs :
101 - 150 of 485 citations for Mouse Anti Zika Virus NS1 Antibody B4 Biotin Conjugate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used in the study were: anti-acetylated lysine (PTM Biolabs, 1:1000 ...
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bioRxiv - Physiology 2023Quote: ... Primary antibodies used targeted pan anti-β-hydroxybutyryllysine (PTM Biolabs, PTM-1201) or anti-amyloid-β1-16 (BioLegend 6E10 ...
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bioRxiv - Genomics 2020Quote: ... biotin-dNTPs at the DNA termini were removed with 500 units of Exonuclease III (NEB #M0206) in 1X NEBuffer 1 (NEB #B7001 ...
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bioRxiv - Biophysics 2020Quote: ... the 5’ end of the 4 kb transcript was biotin-labeled using Vaccinia Capping System (NEB) and 3-biotin-GTP (NEB ...
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bioRxiv - Biophysics 2020Quote: ... A 478 bp fragment of biotin labelled DNA was produced by PCR with Taq Polymerase (NEB) of λ-DNA using the following primers ...
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bioRxiv - Microbiology 2020Quote: ... Biotin-labeled RNP lysates were supplemented with streptavidin beads (1 mL per replicate, New England Biolabs) and captured by rotating at room temperature overnight ...
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bioRxiv - Genomics 2021Quote: ... biotin was removed from the unligated ends by adding 5 µl of T4 DNA polymerase (NEB) to 5 ug of DNA ...
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bioRxiv - Microbiology 2021Quote: ... in the 5’end of 30 mer RNA substrate we used the vaccinia virus capping enzyme following the manufacturer’s protocol (New England Biolabs), except that the methyl donor SAM was absent and 0.05 units of inorganic pyrophosphatase (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2 -O-methyltransferase (New England Biolabs). The mRNA was purified by oligo-dT affinity purification ...
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bioRxiv - Microbiology 2022Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2’-O-methyltransferase (New England Biolabs). The mRNA was purified by oligo-dT affinity purification ...
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bioRxiv - Microbiology 2024Quote: ... the cap-1 structure was added using the vaccinia virus capping enzyme and 2L-O-methyltransferase (New England Biolabs). The mRNA was purified ...
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bioRxiv - Plant Biology 2023Quote: ... The precipitates were eluted into 30μL 1×SDS loading buffer and detected using anti-Myc and anti-MBP antibody (1:5,000, New England Biolabs).
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bioRxiv - Plant Biology 2022Quote: ... Each MBP-tagged RAPTOR1B fragment was detected with anti-MBP antibodies (NEB, E8032S). For the in vitro kinase assay ...
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bioRxiv - Microbiology 2021Quote: ... The membrane was probed with horseradish peroxidase (HRP)-conjugated anti-MBP antibody (NEB) and HRP-conjugated anti-FLAG antibody to detect MBP-TcpB and FBXO22 ...
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bioRxiv - Biochemistry 2021Quote: ... membranes were probed with rabbit polyclonal anti-pansuccinyllysine antibody (PTM Biolabs, PTM-401) at a dilution of 1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Biotin-dNTPs at the unligated DNA termini were removed by ∼500 units of Exonuclease III (NEB #M0206) in 1X NEBuffer 1 (NEB #B7001 ...
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bioRxiv - Cell Biology 2019Quote: ... 1 µl of 1 mM BG-(PEG)12-biotin (New England Biolabs; PEG linker available on request) was added to 200 µl of axonemes ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µl of 1 mM BG-(PEG)12-biotin (New England Biolabs; PEG linker available on request) was added to 200 µl of axonemes ...
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bioRxiv - Molecular Biology 2023Quote: ... SNAP-tag coupling activity (methodology described below) was first tested with a small ligand BG-biotin (NEB) or DNA (a 550 bp benzylguanylated PCR product ...
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 ul 10 mM dGTP, 1.5 ul 10 mM dTTP, 37.5 ul 0.4 mM biotin-14-dATP, 40 ul NEB buffer2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Digested DNA fragments were filled in with biotin-labeled dATP by incubating with Klenow enzyme (NEB, M0212), biotin-14-dATP ...
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bioRxiv - Immunology 2024Quote: ... the post-capture PCR of the sample-bead conjugate was performed with 25 μL LongAmp Taq 2x Hot Start Master Mix (New England Biolabs, M0533), 1 μL 10 μM forward (10x_cDNA_fwd ...
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bioRxiv - Molecular Biology 2020Quote: ... Plasmids encoding HA-tagged LC3B proteins were generated by replacing the EGFP sequence in the EGFP plasmids with an HA-tag followed by a Tobacco etch virus protease sequence recognition site and a Flag-Tag (Genewiz) using AgeI and HindIII (New England Biolabs). Lipidation-deficient LC3B proteins were generated by mutagenic PCR using Q5 Hot Start High-Fidelity Master Mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was synthesized from 500 ng of total RNA with retrotranscriptase from Moloney murine leukemia virus (Ref. M0253, New England BioLabs) and random hexanucleotides as primers by following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The cap 0 standard was generated by treating the 5’-triphosphate/3’-FAM-labeled 25mer with Vaccinia virus capping enzyme (VCE, NEB) in the presence of GTP and S-adenosylmethionine (SAM) ...
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bioRxiv - Biochemistry 2020Quote: ... a maltose binding protein (MBP) and a Tobacco Etch Virus (TEV) protease cleavage site in the N-terminal via Gibson assembly (New England Biolabs) as per the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... mutations were inserted into the open reading frame of Vesicular stomatitis virus glycoprotein VSV-G using the Q5 SDM kit (NEB). Myc epitope-tagged SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Microbiology 2021Quote: ... The DNA depleted RNA was used as the template for reverse transcription performed with Moloney murine leukemia virus (MMLV) reverse transcriptase (M0253, NEB). The cDNA samples were then diluted 1:4 in water and used in quantitative real-time PCR with genespecific primers using SsoAdvanced Universal Sybr green Supermix (1725271 ...
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bioRxiv - Microbiology 2021Quote: ... Virus titer was determined using SYBR-based one step qRT-PCR with Luna Universal One Step qRT-PCR reagent (NEB). QuantStudio 5 (Applied Biosystems ...
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bioRxiv - Microbiology 2020Quote: ... The DNA depleted RNA was used as the template for reverse transcription performed with Moloney murine leukemia virus (MMLV) reverse transcriptase (M0253, NEB). The cDNA samples were then diluted 1:4 in water and used in quantitative real-time PCR with gene-specific primers using SsoAdvanced Universal Sybr green Supermix (1725271 ...
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bioRxiv - Developmental Biology 2022Quote: ... using 200-300 ng of purified RNA as template and Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase (M0253, NEB). cDNA was synthesised using the standard first strand synthesis protocol with random hexamers (S1230 ...
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bioRxiv - Plant Biology 2022Quote: All ObANS genes were inserted into the pBI121 plasmid under the control of the constitutive Cauliflower mosaic virus 35S promoter using the SacI and XbaI restriction enzymes (Thermo-scientific) and the NEBuilder ligation system (NEB). The plasmids were transformed into Agrobacterium tumefaciens strain GV3101 using electrotransformation and positive colonies were selected with kanamycin ...
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bioRxiv - Molecular Biology 2023Quote: ... A CRISPR array containing two repeats and one spacer targeting the gfp gene of recombinant Sindbis virus (SINV-GFP) was generated by annealing and extending two partially complementary DNA oligos with Q5 polymerase (NEB) (Table S1) ...
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bioRxiv - Physiology 2023Quote: ... was obtained by reverse transcription (RT) using 1 μg of RNA and Moloney murine leukaemia virus (M-MuLV) reverse transcriptase (M0253, NEB), using the first strand cDNA synthesis standard protocol with random primers (S1330 ...
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bioRxiv - Microbiology 2024Quote: The ectodomains of the hemagglutinin proteins from selected influenza virus strains were ordered as synthetic DNA fragments from Twist Biosciences and cloned with a barcoded fragment encoding the last 46 amino acids of WSN HA as 3-segment assembly reaction into a construct containing the 3’ non-coding region of the packaging signal and signal peptide from A/WSN/1933 influenza HA and the a Read 1 Illumina sequence and the full 5’ packaging signal from A/WSN/1933 virus using Hifi Assembly Mastermix (NEB). The backbone for this cloning reaction was a pHH21 plasmid (16 ...
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bioRxiv - Microbiology 2024Quote: ... hepatitis delta virus (HDV) ribozyme and simian virus 40 (SV40) poly-A sequence using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) at 50 °C for 60 min ...
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bioRxiv - Biochemistry 2023Quote: ... modified to include a tobacco etch virus (TEV) protease recognition sequence downstream of the 6xHis-tag47 using NEBuilder HiFi DNA assembly master mix (New England BioLabs). Sequences were verified by Sanger DNA sequencing [Genomics Shared Resource ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 30 minutes in PBS containing the primary antibodies: rabbit polyclonal anti-lactyllysine antibody (PTM-1401, PTM Biolabs, Hangzhou, China), mouse monoclonal anti-tubulin β3 (801201 ...
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bioRxiv - Biochemistry 2023Quote: ... Bound protein was detected by western blot using mouse anti-MBP diluted 1:10,000 (New England Biolabs), fluorescent secondary antibodies (Li-Cor Biosciences) ...
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bioRxiv - Microbiology 2019Quote: ... followed by Alexa Fluor 488 goat anti-rabbit secondary antibody (New England Biolabs, UK) for 1h ...
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bioRxiv - Developmental Biology 2024Quote: ... Fgfrb-eGFP signal was detected using an anti-GFP antibody (TP401, Torrey Pine Biolabs) in combination with nuclear (Hoechst ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were then incubated for 1 hour with anti-MBP antibody (New England BioLabs) solution diluted in calcium containing blocking buffer ...
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bioRxiv - Developmental Biology 2020Quote: ... 37.5 μl of 0.4 mM biotin-14-dATP and 10 μl of 5 U/ μl Klenow (NEB, M0210L) were added and mixed by pipetting ...
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bioRxiv - Biophysics 2021Quote: ... The SNAP tag on Nup358min-zip and Nup358min was biotinylated with SNAP-biotin substrate (New England BioLabs, MA) as described99.
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bioRxiv - Biophysics 2019Quote: ... Biotin- and digoxygenin-labeled handles were amplified with a non-proofreading Taq DNA polymerase (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biophysics 2019Quote: ... Biotin- and digoxygenin-labeled handles were amplified with a non-proofreading Taq DNA polymerase (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Biophysics 2019Quote: ... 27P-XhoI-A and XbaI-A oligonucleotides (Table S1) were biotin or digoxigenin tailed using Terminal Transferase (NEB) and either BIO-dUTP or DIG-dUTP ...
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bioRxiv - Molecular Biology 2024Quote: ... The biotin-dNTP at unligated ends was then removed by incubation with 200 U Exonuclease III (NEB, M0206S) in 1× NEB buffer 1 (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... DNA synthesis was visualized by detecting biotin with the Phototope-Star detection kit (New England Biolabs Ref N7020S) and images acquired on a Chemidoc system (Biorad).
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bioRxiv - Microbiology 2020Quote: ... and in vitro bacterial cultures was used as template in reverse transcription reactions using the Murine Moloney Leukemia Virus Reverse Transcriptase (NEB M0253S) following the manufacturer’s protocol ...