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Citations for New England Biolabs :
101 - 150 of 961 citations for Goat Anti Mouse IgG Fc Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Precipitated RNA 3’ends were first dephosphorylated by shrimp alkaline phosphatase (SAP) (NEB M0371), and 3’ RNA linker (20 µM ...
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bioRxiv - Genomics 2020Quote: ... The PCR products were treated with exonuclease I and alkaline phosphatase (NEB, M0293; M0371) and Sanger sequenced to verify successful knockouts ...
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bioRxiv - Neuroscience 2020Quote: ... AP fusion proteins are quantified using calf intestinal alkaline phosphatase (CIP, New England Biolabs) activity ...
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bioRxiv - Cell Biology 2021Quote: ... was digested with BbsI and dephosphorylated using calf intestinal alkaline phosphatase (CIP) (NEB, M0290S). The diluted phosphorylated oligonucleotide duplex was ligated into the dephosphorylated px330 plasmid using a T4 ligase (New England Biolabs ...
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bioRxiv - Developmental Biology 2022Quote: ... Beads were washed with 1x and 5x PMPG and treated with alkaline phosphatase (NEB) to de-phosphorylate DDX43-crosslinked RNA on beads ...
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bioRxiv - Microbiology 2021Quote: ... Digested plasmid was then de-phosphorylated with Shrimp Alkaline Phosphatase (rSAP) (New England Biolabs) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: Purified RNAs were treated with alkaline phosphatase (Calf Intestinal Phosphate from New England Biolabs) at 50°C for 15min followed by heat inactivation at 95°C for 2.5min ...
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bioRxiv - Genetics 2019Quote: ... for 9 h and treated with 2 μl of calf intestinal alkaline phosphatase (NEB) at 37 °C for 30 min ...
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bioRxiv - Genetics 2020Quote: ... The purified linear plasmid was then dephosphorylated using Alkaline Phosphatase Calf Intestinal (CIP) (NEB) to ensure the linear plasmid did not ligate with itself ...
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bioRxiv - Molecular Biology 2021Quote: ... in the presence of 5 U of Quick-CIP alkaline phosphatase (New England Biolabs). After dialysis ...
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bioRxiv - Genetics 2022Quote: ... The pCEP4 backbone was dephosphorylated using Calf Intestinal Alkaline Phosphatase (CIP) (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... SpoX or SpoX* was dephosphorylated using 25 U of calf intestine alkaline phosphatase (NEB) in a 50 μL reaction volume and incubated for 1 h at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... the beads were washed and incubated with 50U shrimp alkaline phosphatase (New England Biolabs) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Fragments were dephosphorylated with 20 units Shrimp Alkaline Phosphatase (rSAP, M0371, New England Biolabs) for 1 hour at 37°C with 1 μl RNaseOUT (10777019 ...
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bioRxiv - Bioengineering 2023Quote: ... digested with Bbs1 and treated with alkaline phosphatase (Thermo) using T4 DNA ligase (NEB). The SaCas9 gRNA expression plasmid (pIBH072 ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated with 50 units of alkaline phosphatase (calf intestinal, QuickCIP, New England BioLabs) overnight at 37 °C while shaking ...
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bioRxiv - Systems Biology 2023Quote: ... and de-phosphorylating it with Quick CIP alkaline phosphatase (New England Biolabs, Ipswitch, MA); 2 ...
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bioRxiv - Microbiology 2019Quote: ... was obtained by adding 100 units of calf-intestinal alkaline phosphatase (CIP) (New England Biolabs) and 2mg/ml RNase A (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... Pure fractions were mixed with 500 units Quick Calf Intestinal Alkaline Phosphatase (New England BioLabs) per 1 mg SMARCAD1 for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... at 37°C for 30 min and dephosphorylated by 1U of Shrimp Alkaline Phosphatase (NEB) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... and 3 µg/ml leupeptin were incubate with 17.5 U alkaline phosphatase (New England Biolabs) at 37°C for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... The plasmid was cut and de-phosphorylated simultaneously using 1 U Shrimp Alkaline Phosphatase (NEB) and 20 U EcoRV-HF (NEB ...
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bioRxiv - Microbiology 2019Quote: ... resuspended in 100 μl CIP buffer with 10 units of calf intestinal alkaline phosphatase (NEB) and incubated for 30 min at 37°C in a heat block ...
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bioRxiv - Microbiology 2020Quote: ... 30 mM acetyl phosphate or 1 µl Calf Intestinal Alkaline Phosphatase (CIP) (NEB, catalog#: M0290V) was added to the solution ...
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bioRxiv - Microbiology 2020Quote: ... resuspended in 100 μl CIP buffer with 10 units of calf intestinal alkaline phosphatase (NEB) and incubated for 30 min at 37°C in a heat block ...
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bioRxiv - Genomics 2020Quote: ... input RNA was dephosphorylated by incubating with Calf Intestinal Alkaline Phosphatase (0.3U/μl) (CIP)(NEB) at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.125 μl of Shrimp Alkaline Phosphatase (1 U/ml; New England Biolabs, cat. no. M0371S), 0.0125 μl Exonuclease I (20 U/ml ...
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bioRxiv - Plant Biology 2022Quote: ... 5 pmol of label-free RNA substrates were dephosphorylated with Alkaline Phosphatase (New England Biolabs) and labeled with [γ-32P] ATP (PerkinElmer ...
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bioRxiv - Systems Biology 2023Quote: ... The backbone was de-phosphorylated with Quick CIP alkaline phosphatase (New England Biolabs, Ipswitch, MA). All components were ligated together using NEB T4 Ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatants were immunoprecipitated overnight with 40 µL of precoated anti-IgG magnetic beads (goat anti-rabbit IgG magnetic beads, NEB) previously incubated with the antibody of interest for 4 h at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... the plasmid digestion was supplemented with 1.5 units of recombinant shrimp alkaline phosphatase (rSAP; NEB #M0371S). Digested insert and vector were purified and ligated together at room temperature for 30 min using T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... Typically 5ug of HMW DNA was subjected to incubation using Shrimp Alkaline Phosphatase (rSAP; NEB, M0371) for 30min at 37°C followed by 5min at 65°C to dephosphorylate DNA free ends ...
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bioRxiv - Biochemistry 2019Quote: ... To obtain the nucleotide free (apo) state 50 U/mL of alkaline phosphatase (New England Biolabs) was used ...
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bioRxiv - Molecular Biology 2020Quote: ... ExoSAP was done by adding 0.125 µl Shrimp Alkaline Phosphatase (1U/µl; New England Biolabs, M0371S), 0.0125 µl Exonuclease I (20 U/µl ...
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bioRxiv - Genomics 2022Quote: ... The pCEP4 backbone was dephosphorylated using Calf Intestinal Alkaline Phosphatase (CIP) (New England Biolabs, Cat#: M0290). The backbone and fragments of either miPSC_1_L1 or miPSC_4_L1 were combined in a single ligation reaction using T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Molecular Biology 2019Quote: ... These fragments were dephosphorylated with 30 units of recombinant shrimp alkaline phosphatase (M0371, NEW ENGLAND BIOLABS) for 1 hour at 37 °C with RNasin Plus (N2611 ...
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bioRxiv - Microbiology 2020Quote: ... either 30 mM acetyl phosphate or 0.5 μl Calf Intestinal Alkaline Phosphatase (CIP) (NEB, catalog#: M0290V) was added to the solution ...
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bioRxiv - Plant Biology 2022Quote: ... The resultant DNA fragments were treated with Exonuclease I and Shrimp Alkaline Phosphatase (New England Biolabs), purified with Fast GeneTM Gel/PCR Extraction Kit (NIPPON Genetics Co. ...
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bioRxiv - Molecular Biology 2022Quote: ... and 5 µg of HMW DNA were de-phosphorylated using Shrimp Alkaline Phosphatase (rSAP; NEB, M0371) for 30 min at 37°C followed by 5 min at 65°C ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant SYNJ2BP (0.5 µg) was dephosphorylated using 1 µl of calf intestinal alkaline phosphatase (CIP) (NEB) in 1x CutSmart Buffer in a total volume of 10 µl ...
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The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... 100 µL of CIP-mix containing 10 U of calf intestinal alkaline phosphatase (New England Biolabs) in CIP-buffer were added and beads incubated for 30 min at 37°C ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: GBL phosphate (8) was incubated with 5 units of calf intestinal alkaline phosphatase (CIP, New England Biolabs) in 50 mM HEPES buffer (pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... was sequentially digested with BamH1 and Kpn1 followed by dephosphorylation with shrimp alkaline phosphatase (New England Biolabs). Ligation of the product and linearized vector was performed overnight at 14°C with T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... The pBluescript plasmid was cut by EcoRV and dephosphorylated by Calf Intestinal Alkaline Phosphatase (CIP) (NEB, M0290L), then ligated with the phosphorylated PCR fragments by T4 DNA Ligase (NEB ...
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bioRxiv - Immunology 2020Quote: ... The amplified DNA was then treated with recombinant Shrimp Alkaline Phosphatase and Exonuclease 1 (New England Biolabs), and sent to ELIM Biopharm for sequencing ...
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bioRxiv - Microbiology 2020Quote: ... beads were resuspended in 200 μl CIP mix (20 units of calf intestinal alkaline phosphatase (M0290, NEB) in CIP buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 µl freshly made 1 M NH4HCO3 and calf intestinal alkaline phosphatase (1 U, New England Biolabs) were added ...
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bioRxiv - Cell Biology 2023Quote: ... and the vector was treated with Mung Bean Nuclease and Alkaline Phosphatase Calf Intestinal (New England Biolabs). The blasticidin resistance gene was isolated from a plasmid pKM808 using XhoI/SalI (New England Biolabs ...
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bioRxiv - Bioengineering 2023Quote: ... the vector was dephosphorylated by the addition of 2 U of shrimp alkaline phosphatase (New England BioLabs) for the last 30 min of the restriction reaction ...
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bioRxiv - Biochemistry 2023Quote: ... guide RNAs were first dephosphorylated using shrimp alkaline phosphatase (rSAP, 0.05 U/µL; New England Biolabs, M0371) at 37 °C for 30 min ...