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Citations for New England Biolabs :
101 - 150 of 3242 citations for Glyphosate Chemical Purity 96% 2 13C 99%; 15N 98+% 100 Ug Ml In H2O since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... Chemical competent NEB5α-cells (NEB biolabs) were used for propagation of plasmid DNA after Q5-mutagenesis ...
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bioRxiv - Plant Biology 2023Quote: ... and amplified using NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 2, New England Biolabs, #E6442). After quality check with the Bioanalyzer High Sensitivity DNA assay (Agilent) ...
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bioRxiv - Molecular Biology 2019Quote: 2 µg of genomic DNA was digested with 100 units of MspI (NEB R0106M) or TaqαI (NEB R0149M ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin was digested overnight (ON) with either 2 μl (100 U) Dpn II (NEB) (later experiments ...
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bioRxiv - Neuroscience 2021Quote: Libraries were prepared from 1 ug RNA using a library prep kit (NEB #7770), rRNA depletion kit (NEB #E6310) ...
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bioRxiv - Microbiology 2021Quote: ... acnes lysates were incubated with 0.5 ug plasmid DNA together with different buffers (NEB) supplemented with or without ATP or SAM ...
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bioRxiv - Cancer Biology 2019Quote: ... and then resuspended in sorting buffer (PBS with 0.5% BSA, 2.5 mM MgCl2, 0.5 mM CaCl2, 1 µg/mL DAPI and 100 U/mL DNaseI, NEB) and incubated for 30 minutes at room temperature before sorting ...
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bioRxiv - Genomics 2021Quote: ... 12 μL of 10 mg/mL Bovine Serum Albumin (100× BSA, NEB), 5 μL of 400 U/μL T4 DNA Ligase (NEB M0202) ...
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bioRxiv - Genomics 2023Quote: ... 12 μL of 10 mg/mL Bovine Serum Albumin (100 × BSA, NEB), 5 μL of 400 U/μL T4 DNA Ligase (NEB #M0202) ...
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bioRxiv - Microbiology 2020Quote: ... Enzymatics) and end repaired Silane purified cDNA eluted in in 32 μl of RNase free H2O (1x NEB buffer 2 ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified DNA was eluted in 17 μL H2O and treated with exonuclease (New England Biolabs, ordering info missing). Subsequently ...
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bioRxiv - Biochemistry 2020Quote: ... (1.5 μM x 2.5 μL in H2O) followed by addition of 4.5 μL NEBNext Ultra II Pol master mix (NEB). The reaction mixture was denatured at 98 °C for 1 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... One acceptor and one or more donors were mixed with a ratio of 1:1.1 in distilled H2O with 0.5 ul (7.5 U) of CRE recombinase (NEB # M0298M) and 1 ul Cre buffer (provided with CRE recombinase ...
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bioRxiv - Genomics 2021Quote: ... 50 µl of ULR-Phusion mix (1.2 µl ULR Ladder, 28.8 µl H2O, 30 µl Phusion PCR mastermix [New England Biolabs] and the samples were mixed with 147.5 µl ProNex chemistry ...
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bioRxiv - Microbiology 2021Quote: ... primers that contained restriction sites for BamHI and XmaI or XbaI (Table S5, primers No. 9-16 and 95-98) and the high-fidelity polymerase Q5 (New England Biolabs) according to vendor’s manual ...
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bioRxiv - Bioengineering 2022Quote: ... 1 μL of 2-Log DNA Ladder (200 μg/mL; NEB) is mixed with 1 μL of Gel Loading Dye (6x ...
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bioRxiv - Genomics 2021Quote: ... 2 μL of 1 mg/ml bovine serum albumin solution (NEB), 1 μL of hOGG1 (ProSpec TechnoGene Ltd. ...
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bioRxiv - Microbiology 2020Quote: ... after which 2 µL calf intestinal phosphatase (10,000 U/mL, NEB) was added to the digest and incubated for 1h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... An additional 2 μl RNase A (20 mg/ml, NEB #T3018) was added and followed by 5 min incubation at 56 °C to remove residual RNA in the sample ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 ul of micrococcal nuclease (Biolabs M02475, 2×106 U/ml) was added to 200 μl buffer containing 1 mM CaCl2 and incubated for 10 min 37°C ...
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bioRxiv - Genetics 2023Quote: ... diluted to 2 mg/ml in Nuclease-Free Water (NEB, B1500L), at 37°C for 15 minutes each ...
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bioRxiv - Immunology 2021Quote: ... except after the Ni-NTA step it was concentrated (1 mg/ml in 0.2 ml) and shaved with endoglycosidase D (10 μl, 100 unit) (New England BioLabs) for 16 hrs at 4°C prior to gel filtration ...
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bioRxiv - Genomics 2021Quote: ... 96 μM S-adenosylmethionine (NEB) and 200 U of M.CviPI (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... The sample was then resuspended in 100 μl/sample volume of 1X NEBuffer 2 (NEB) supplemented with Triton X-100 to 0.1% and transferred to a new tube ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 ug from each cell line was digested with 25 units EcoRI (New England Biolabs) in 100 ul total volume ...
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bioRxiv - Molecular Biology 2023Quote: ... Four ug of fragmented gDNA was digested with 20 U of RNase H (NEB #M0297S) at 37°C for 6 hours to serve as a negative control ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg/mL cycloheximide) supplemented with 0.1 U/µL murine RNase Inhibitor (NEB), and passed through a G25 needle 25 times ...
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bioRxiv - Cell Biology 2022Quote: ... followed by 2 μL of 20 mg/mL RNaseA (New England BioLabs) for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was then passed over a 2 mL amylose column (NEB) and washed with 3 CV of Flag Wash Buffer ...
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bioRxiv - Biochemistry 2020Quote: ... 186 bp DNA (0.4 mg/ mL) in 1x NEBuffer 2 (NEB: B7002) was incubated with dATP (100 μM) ...
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bioRxiv - Biochemistry 2022Quote: ... and further passivated with 2 mg/ml Bovine serum albumin (BSA, NEB:B9000S) to prevent non-specific binding of proteins and DNA to the surface ...
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bioRxiv - Bioengineering 2022Quote: ... followed by 2 μL of 20 mg/mL RNaseA (New England BioLabs) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was rocked with 2 mL of amylose resin slurry (NEB) for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by 2 µL of 20 mg/mL RNaseA (New England BioLabs) for 30 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... 2 μL of T4 RNA Ligase (10 000 U/mL, NEB, M0204S), and 10 μL of PEG 8000 50% (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... and protein purity and concentration were analyzed along with T7 RNAP (New England Biolabs, #M0251S) by 10% SDS-PAGE gel electrophoresis stained with Coomassie blue (Bio-rad).
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bioRxiv - Molecular Biology 2021Quote: ... 5 µL of linker ligation mixture was added (38% PEG-8000, 1x NEB T4 ligase buffer, 2 µM oBZ407_preA preadenylated linker, 100 units NEB T4 RNA ligase 2, truncated) and they were further incubated at 37 °C for 3 hrs ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was purified with Silane beads and eluted in 52 μl of RNase free H2O as input for end repair reaction using End Repair Module (NEB) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2019Quote: ... up to 500 ng of gDNA was incubated in 10 µL H2O with 1x CutSmart buffer and 0.5 U rSAP (New England BioLabs #M0371L) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were resuspended in H2O used for library preparation with the NEBNext Ultra II Library Prep kit for Illumina (NEB). Beads were then washed 4 times using Tween wash buffer (5 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... gRNAs were annealed in (1 μl Forward primer, 1 μl Reverse primer, 5 μl Buffer 4 NEB, 43 μl H2O) by heating at 98 °C for 5 min and allowing the tubes to cool down to room temperature in the thermoblock (∼3h) ...
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bioRxiv - Microbiology 2020Quote: ... In the meantime, we prepared 150 μl of T4 PNK mix (120 μl H2O, 20 μl 10x T4 PNK buffer (NEB), 1 μl Murine RNase Inhibitor (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... before resuspending the beads in 25 μl RNA ligation mix (9 μl H2O, 3 μl 10x T4 RNA ligase buffer (NEB), 0.3 μl 0.1M ATP ...
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bioRxiv - Cell Biology 2021Quote: ... according to the manufacturer’s instructions (with an elution in 30 μL sterile filtered MilliQ H2O) and restriction digest with suitable restriction enzymes (New England Biolabs) of about 250 ng plasmid DNA for 3 h ...
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bioRxiv - Biochemistry 2023Quote: ... DNA pellets were suspended in 50 μl of H2O and 10 μg of DNA was digested by EcoRI restriction endonuclease (NEB) at 37 °C for 1 h ...
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bioRxiv - Synthetic Biology 2024Quote: ... The coding and reverse oligonucleotides were mixed (6 µL H2O, 1 µL T4 Ligase Buffer, 1 µL T4 PNK (10 U/µL; NEB), 1 µL of each 100 µM oligonucleotide ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... proteins (2 mg mL-1) were incubated with 180000 U mL-1 PNGase F (New England Biolabs, Unites States) in PBS (pH 7.4 ...
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bioRxiv - Genomics 2022Quote: ... 3 ug of input DNA was dephosphorylated with Quick CIP (New England Biolabs, cat no M0508). Following enzyme inactivation with alkaline phosphatase ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were further quenched by the addition of 0.2 ug of RNAse A (New England Biolabs) and incubation at 37 °C for 20 min ...
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bioRxiv - Genomics 2019Quote: ... we used the miRNA quantifications from all brain libraries with all starting amounts using both batches (total of 99 libraries, 19 for the Clontech, NEXTflex, Deduped and Fivepercent methods and 10 for Illumina and 13 for NEB). We normalized the data using the DESeq2(40 ...