Labshake search
Citations for New England Biolabs :
101 - 150 of 357 citations for Adenovirus Type 40 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2021Quote: ... 7 μg pNZdmsC3GH plasmid was digested with 40 U of SfiI (NEB), separated on a 1% agarose gel and ...
-
bioRxiv - Plant Biology 2022Quote: ... incubated with λ protein phosphatase (40 units/μL) (P0753S, New England Biolabs) or recombinant TOPP4-GST/HIS for 1 h at 30□ ...
-
bioRxiv - Microbiology 2022Quote: ... 0.1% NP-40 in NFW) and using a magnetic rack (NEB #S1506),the buffer was decanted after equilibration ...
-
bioRxiv - Molecular Biology 2024Quote: ... 50 μg/ml BSA) (12) and 40 U Murine RNase Inhibitor (NEB). Aliquots were withdrawn after 3 and 30 minutes at 25 °C ...
-
bioRxiv - Genomics 2024Quote: ... and the top two scoring sgRNAs for each target gene based on depletion or enrichment phenotypes were cloned into dual sgRNA lentivirus expression vectors with direct capture tags (Addgene 187241)40 using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs #E2621L) (Table S2) ...
-
bioRxiv - Molecular Biology 2021Quote: pUC19 was digested using thirteen commercially available Type II restriction enzymes (New England Biolabs). For BamHI ...
-
bioRxiv - Bioengineering 2022Quote: ... The template DNA was removed by using DpnI type IIM restriction enzyme (NEB, USA) at 37°C (1 – 2 h ...
-
bioRxiv - Molecular Biology 2022Quote: ... and/or 10 U of T4 PNK (wild-type or 3’ phosphatase minus, NEB). For RtcB ligation ...
-
bioRxiv - Biophysics 2022Quote: ... type IIS enzymes (BsaI, New England Biolabs #R3733, and BsmBI, New England Biolabs #R0739) were used to iteratively concatenate sequence modules ...
-
bioRxiv - Developmental Biology 2022Quote: ... along with enzymatic digestion of the wild-type amplicon with enzyme BslI (NEB R0555S). Genotyping dmrt2bumb4required treatment with the enzyme BaeGI (NEB R0708S) ...
-
bioRxiv - Biophysics 2023Quote: ... the use of a Type IIs restriction enzyme (BsaI-HF-v2, New England Biolabs) that produces unique 4 bp sticky ends ensures that the three fragments are oriented in a unique way.
-
bioRxiv - Microbiology 2023Quote: ... Genes of interest were amplified from wild-type genomic DNA using Phusion Polymerase (NEB), according to the manufacturer’s guidelines ...
-
bioRxiv - Developmental Biology 2024Quote: ... R0148S)2 or the wild-type allele was digested with HphI (New England Biolabs, R0158S) for 1 hour ...
-
bioRxiv - Cell Biology 2020Quote: 5′ end phosphorylation and radiolabeling (1x PNK buffer, 40 U T4 PNK (NEB), 40 μCi 32P-γATP ...
-
bioRxiv - Cell Biology 2020Quote: 5′ linker ligation (1x PNK buffer, 40 U T4 RNA ligase I (NEB), 80 U RNasIN ...
-
bioRxiv - Molecular Biology 2020Quote: ... 30 to 40 μg of genomic DNA was digested with HinfI (NEB R0155) and RsaI (NEB R0167) ...
-
bioRxiv - Molecular Biology 2020Quote: 5’ linker ligation (1x PNK buffer, 40 u T4 RNA ligase I (NEB), 80 u RNaseOUT ...
-
bioRxiv - Microbiology 2022Quote: ... 0.5 μl of deoxynucleotide (dNTP) solution mix (40 μM; New England Biolabs, UK), 1 μl of forward primer (10 μM ...
-
bioRxiv - Genomics 2023Quote: ... 160 µL of 40 U µL-1 Taq DNA ligase (New England Biolabs) and 700 µL of PCR-grade H2O ...
-
bioRxiv - Genetics 2024Quote: ... 40 μl of MNAse digestion buffer (3.33 gelUnits/μl Micrococcal Nuclease (NEB M0247S), 1.25X Micrococcal Nuclease buffer ...
-
bioRxiv - Biochemistry 2019Quote: ... This assembly was performed using a Type IIs restriction enzyme BsaI (New England Biolabs # R3535) and T4 DNA ligase (New England Biolabs # M0202 ...
-
bioRxiv - Genomics 2020Quote: KlenTaq wild type gene was amplified using a high fidelity PCR (Q5 polymerase from NEB) from a stored plasmid ...
-
bioRxiv - Biochemistry 2022Quote: ... wild-type CSF3R MIG plasmid and insert were digested with SfiI (New England Biolabs R0123S), ligated overnight with a 3:1 molar ratio ...
-
bioRxiv - Molecular Biology 2022Quote: ... the protein coding sequences were ordered synthetically with Part 3b type BsaI overhangs (NEB R3733) and cloned into the entry vector with BsmBI (NEB R0739) ...
-
bioRxiv - Systems Biology 2023Quote: ... all wild-type bZIP intermediate plasmids were digested with AvrII (New England Biolabs, Ipswitch, MA) and HindIII-HF (New England Biolabs ...
-
bioRxiv - Synthetic Biology 2024Quote: pSpy0C2 was cloned using Golden Gate Assembly using the type II restriction enzyme Esp3I (NEB). The MCS-cat fragment and the pUC origin were amplified using OLEC10311/10312 from pSpy1C and OLEC10315/10316 from pUC19-mKate2∼ssrA ...
-
bioRxiv - Molecular Biology 2021Quote: ... cell lysates were incubated in 40 μg/ml PNGase F (New England Biolabs, MA) for 2 hr at 37 °C to cleave all N-glycans before immunoblotting 62 ...
-
bioRxiv - Biochemistry 2021Quote: ... After slowly cooling down T4 DNA ligase (final concentration of 40 U/mL, NEB) was added and the reaction was performed for 2 h at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: ... 40 μg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C to remove of biotin from non-ligated fragment ends ...
-
bioRxiv - Biophysics 2021Quote: ... we linearized 40 µg of the vector with NotI (R0189S, New England Biolabs, MA), mixed the linearized plasmid with 5×107 cells that we had washed with 10 mL of cytomix (120 mM KCl ...
-
bioRxiv - Molecular Biology 2019Quote: ... 40 µg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C ...
-
bioRxiv - Cell Biology 2019Quote: ... immunoprecipitated beads were incubated with 40 Units of lambda protein phosphate (New England Biolabs) in a 50 μl reaction ...
-
bioRxiv - Biochemistry 2021Quote: ... and 0.02% NP-40) including 1 μL of PstI (NEB, at 100,000 U/mL), 2 mM ATP (Sigma-Aldrich) ...
-
bioRxiv - Genomics 2019Quote: ... 40 μg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C ...
-
bioRxiv - Biophysics 2023Quote: ... 0.05% (v/v) Tween 20 (SigmaAldrich) and 40 mg/mL BSA (New England Biolabs).
-
bioRxiv - Biophysics 2023Quote: ... 0.05% (v/v) Tween 20 (SigmaAldrich) and 40 mg/mL BSA (New England Biolabs), pH 7.9 ...
-
bioRxiv - Cancer Biology 2023Quote: ... A volume of 40 μl reaction mix containing 5 μl isothermal amplification buffer (NEB), 3 μl 100 mM MgSO4 ...
-
bioRxiv - Cell Biology 2023Quote: ... immunoprecipitated beads were incubated with 40 Units of lambda protein phosphate (New England Biolabs) in a 50 μl reaction ...
-
bioRxiv - Biophysics 2023Quote: 12mer arrays (40 ng) were digested with 1 U of MNase (New England Biolabs) in a 10 μL reaction for varying digestion times (2.5 to 10 minutes ...
-
bioRxiv - Genomics 2021Quote: ... gDNA was digested with the CspCI Type IIB restriction enzyme (IIB-REase - New England BioLabs, Inc.) which has shown to yield a high marker density in triatomine65 ...
-
bioRxiv - Cell Biology 2020Quote: β1-tubulin wild type construct was generated by the gibson assembly (HiFi Kit, New England Biolabs) of a TubB1 sequence fragment synthesised as a gBlock by Integrated DNA Technologies (IDT ...
-
bioRxiv - Developmental Biology 2020Quote: ... followed by digestion of the wild-type strand with the enzyme BsurI (New England Biolabs, R0581S)[27] ...
-
bioRxiv - Microbiology 2020Quote: ... The wild-type Chlamydia trachomatis ct276 gene was amplified by PCR with Phusion DNA polymerase (NEB) using 100 ng C ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Golden gate (Type-IIS) cloning was conducted using chemically competent Escherichia coli DH5α cells (NEB, C2987H). Construction of destination vectors with the toxic selection marker ccdB was done with chemically competent One Shot ccdB Survival 2 T1R E ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 750 ng of each sample was digested with 40 U of EcoRI (NEB, R0101L) and 2 μl of 10× EcoRI buffer in a 20 μl reaction for 12 hours ...
-
bioRxiv - Molecular Biology 2022Quote: ... was used for chromatin analysis of nuclei and 40 U of HaeIII (New England Biolabs) was used for secondary cleavage ...
-
bioRxiv - Molecular Biology 2020Quote: ... Beads were resuspended in 40 μL of 1X T4 DNA ligase reaction buffer (NEB, B0202S) containing 375 nM of Second ligation adapter (synthesized by IDT ...
-
bioRxiv - Molecular Biology 2022Quote: 5’ linker ligation (1x PNK buffer, 40 U T4 RNA ligase I (NEB, Cat# M0204L), 80 U RNaseOUT ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... PCR’s were conducted for 40 cycles with the Phusion™ High-Fidelity DNA polymerase (NEB) using an annealing temperature of 65°C.
-
bioRxiv - Immunology 2022Quote: ... cells were digested with Micrococcal Nuclease at 37°C for 40 min (New England BioLabs) and added 10 mM EDTA to stop the reaction ...