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Citations for New England Biolabs :
101 - 150 of 2030 citations for 6 2 Ethoxyphenyl 6 oxohexanoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The resulting mixtures were loaded on a 6% native polyacrylamide gel with SDS-free loading dye (NEB). The gel was made with TAE-Mg buffer (0.4 M Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... Cole) containing an N-terminal 6×His-tag using NEBuilder HiFi DNA Assembly Master Mix (NEB E2621L). The LSD1 constructs were expressed in BL21-CodonPlus (DE3)-RIPL competent E ...
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bioRxiv - Biophysics 2024Quote: ... then mixed with 0.2 volumes of 6× Agarose Gel Loading Dye (Purple, no SDS, New England Biolabs) and analyzed on a 1% agarose gel containing SYBR Safe ...
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bioRxiv - Molecular Biology 2021Quote: ... 6×50 µl PCR reactions were set up using NEBNext Ultra II Q5 master mix (New England Biolabs) with 5 nM oligos as template ...
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bioRxiv - Genetics 2022Quote: ... we PCR amplified NatMX from Addgene plasmid #35121 with the primers listed in Supplementary Table 6 using Phusion Hot Start Flex DNA polymerase (NEB). The NatMX cassette was transformed into the BY strain using the methods described above and transformants were plated onto YPD medium containing clonNAT ...
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bioRxiv - Molecular Biology 2022Quote: ... The final cDNA libraries were amplified (cycle number of 6) using NEBNext Multiplex Oligos for Illumina (NEB, E7335) and purified using SPRIselect size selection beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.6 mM of A, C, and T, NEB-N0446S; 0.1 µM Clean.G; 800 U/ml Bst Polymerase, NEB-M0374L ...
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bioRxiv - Biochemistry 2023Quote: ... and transformed 1 µL of the reaction mixture into 6 µL of BL21 competent cells (New England Biolabs). After heat shock and recovery in SOC media ...
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bioRxiv - Genetics 2024Quote: ... along with the co-injection marker rol-6(su1006) (pRF4) and DNA ladder (1kb DNA Ladder N3232, NEB), generating the three AFD-specific independent lines BFF95 ...
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bioRxiv - Cell Biology 2024Quote: ... Full length human NHSL1-A1 including Exon 1a and excluding exons 3 and 6 was generated by NEB HIFI assembly using a synthetic DNA fragment (gBlocksTM ...
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bioRxiv - Genetics 2024Quote: ... 10 μg of plasmid was digested with 15 units of KpnI-HF and 6 units of FseI (NEB), and the fragment was then run on an agarose gel and the digested fragment was isolated and column purified.
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bioRxiv - Microbiology 2022Quote: ... The resulting nucleic acids were treated with 2 U DNase I (New England Biolabs), 10 U S1 nuclease (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: Toxins were expressed with (His)6-tags at their C-termini in competent C43 (DE3) Escherichia coli cells (NEB) for HlgA and in BL21 (DE3 ...
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bioRxiv - Genetics 2021Quote: ... EagI/KpnI-digested ORFs were ligated into the EagI/KpnI-digested pUASTattB vector backbone using a 6:1 insert:vector molar ratio and T4 DNA ligase (M0202S, NEB) in a thermocycler overnight (∼16 hr) ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 µl of 1xMNase buffer containing 60 gel units/ml (6 Kunz units/ml) of Micrococcal nuclease (NEB M0247S) were added and incubated for exactly for 5 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... and subsequently mixed at a 6:1 ratio with the digested backbone in reactions containing T4 DNA ligase (NEB).
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bioRxiv - Developmental Biology 2020Quote: ... and 6 cDNA libraries were prepared with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) with 9 amplification cycles ...
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bioRxiv - Biophysics 2021Quote: Polymerised gels were removed from the coverslip chamber and placed into 6-well plates to undergo digestion in 0.2 mg/ml proteinase K (New England Biolabs) dissolved in digestion buffer (50 mM Tris pH 8.0 (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... the supernatant was passed twice at a flow rate of 0.5 mL/min through a column with 6 mL chitin beads (NEB) equilibrated with lysis buffer ...
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bioRxiv - Biophysics 2020Quote: ... The point mutants were subsequently cloned in the pNZopuAHis plasmid (C-terminal 6-HIS-tag) using EcoRV-AlnwnI (NEB) for the substrate-binding domain mutants and βamHI-AlwnI (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... 6 μM kifunensine was added to the ExpiCHO culture media at the time of transfection and Endo Hf (NEB) treatment of LC-kappa purified protein (∼10,000 U/mg protein at 4°C for 3 hours ...
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bioRxiv - Evolutionary Biology 2021Quote: ... see Supplementary Data 6) and cloned into pDHFR vector provided in the PURExpress In Vitro Protein Synthesis Kit (NEB). The translation into liposomes was done as described previously95 and the output was analyzed by Blue Native PAGE using 2% digitonin and NativePAGE Novex 4-16% Bis-Tris Protein Gel (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: To generate circular DNA with a single nick phagemid DNA (~2,6 μg) with or without non-denaturing bisulfite treatment was treated with 20 Units of Nt.BstNBI (New England Biolabs) for 1 hour at 37°C in 50 μl of 1X NEBuffer 3.1 followed by deactivation at 70°C for twenty minutes.
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bioRxiv - Microbiology 2021Quote: ... 4°C and the supernatant applied on a 6 ml gravity flow column with Chitin resin (New England Biolabs), equilibrated with 20 mM HEPES ...
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bioRxiv - Synthetic Biology 2022Quote: ... 8 μL of solution A and 6 μL of solution B were mixed with 16 U RNase Inhibitor (NEB), gBlock templates (2 nM each ...
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bioRxiv - Cell Biology 2022Quote: ... Cx36-SNAP transfectants were treated with 6 µM SNAP-Cell Oregon Green (New England Biolabs Inc., Boston, MA, USA) in 10% FBS DMEM for 30 min at 37°C to quench all Cx36-SNAP molecules ...
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bioRxiv - Microbiology 2024Quote: ... protein aliquots were subjected to PNGase F or a broad range mannosidase (α1,2/3/6) (New England Biolabs, France) digestion according to the manufacturer’s specifications ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted from 4.3×10^6 cells using the Monarch HMW DNA Extraction Kit for Cells and Blood (New England Biolabs) with 1400 rpm lysis speed ...
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bioRxiv - Molecular Biology 2023Quote: ... The cDNA was amplified in a 25 µl PCR reactions using 6 µl cDNA and 2X Master mix (NEB). The PCR cycling program was 98 °C for 30 seconds ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 µL 100 µM dNTPs, 0.3 µL 50 mg/mL BSA, 6 µL blunting enzyme mix from quick blunting kit, NEB). Prior to ligation ...
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bioRxiv - Biochemistry 2023Quote: 3 μL of the transcription and co-transcriptional reactions were added to a 6 μL mixture containing 1x DNase I reaction buffer and 0.5 U/μL DNase I (New England Biolabs). After incubation at 37°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... We incubated TXTL reactions at 29 °C for 6 h and digested at 37 °C with PacI (NEB R0547S) as instructed by the provider and added water instead of PacI for the undigested control ...
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bioRxiv - Microbiology 2024Quote: ... Reactions were then DpnI-treated for 6 hours and purified using the Monarch PCR and DNA Cleanup Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... 800 μL of 2 M MgCl2 and 1.6 mL 1 M CaCl2 were added to the lysed cells along with 2.5 μL DNase (New England Biolabs) and OmniCleave endonuclease (Biosearch Technology ...
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bioRxiv - Biophysics 2024Quote: ... the eluate from IgG Sepharose 6 FF was incubated with SNAP-Surface-Alexa Flour 647 (New England Biolabs, S9136S) at 4 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... These DNA samples were then digested at 37°C for 6 h with 100 U MspI (New England Biolabs). Reactions were inactivated with Proteinase K at 42°C for 45 min ...
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bioRxiv - Biochemistry 2020Quote: ... The substrate was designed such that the digested strand is 6 nt shorter than the undigested strand to allow 3′ fill-in by Klenow Fragment (3′-5′ exo-; NEB) with α[32P]-dCTP and cold dATP ...
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Direct analysis of ribosome targeting illuminates thousand-fold regulation of translation initiationbioRxiv - Molecular Biology 2020Quote: ... Gel-purified cDNA products were ligated to the adapter oWG920 (Supplementary Table 6) using T4 RNA ligase 1 (NEB M0437M). cDNA cleanup was performed using 10 µl MyOne Silane beads (Thermo Scientific 37002D ...
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bioRxiv - Synthetic Biology 2020Quote: ... All digested products were column purified and mixed at a 6:1 molar ratio in the presence of T4 DNA ligase (NEB).
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bioRxiv - Microbiology 2021Quote: ... primers oJV5-6 were used to remove ackA from pJV204 using the Q5 site-directed mutagenesis kit (NEB CN# E0554S) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: Wild-type MukB was 6×His-tagged at the C-terminus and was expressed from plasmid Pet21 in C3013I cells (NEB). For Immobilized His6-tagged MukB ...
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bioRxiv - Biochemistry 2021Quote: ... 150 μl of the remaining sample was mixed with 6 μl (1 μl per 25 μl sample volume) of Thermolabile Proteinase K (NEB), incubated at 37 °C for 30 minutes ...
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bioRxiv - Systems Biology 2020Quote: The extracted plasmids were amplified using “lib-seq” primers in the Supplementary Table 6 for 10 cycles using Phusion PCR kit (NEB). Each 50 μL PCR reaction consisted of 6 μL of plasmid library ...
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bioRxiv - Microbiology 2021Quote: ... were PCR-amplified using primers that contained restriction sites for EcoRI and SacI (Table S5, primers No. 1-6) and the high-fidelity polymerase Q5 (New England Biolabs) according to vendor’s manual ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... cDNA was synthesized from 1 μg of total RNA using M-MuLV Reverse Transcriptase and Random Primers 6 (both New England Biolabs) at 42 °C for 60 min and diluted in 1:4 ratio by PCR grade water ...
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bioRxiv - Plant Biology 2020Quote: ... for sgRNA-LecRK-I.1 and pMR218 (L5-L2) for sgRNA-LecRK-I.6 via a cut-ligation reaction with BbsI (New England Biolabs) and T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Neuroscience 2021Quote: ... One piece of mouse frontal cortex tissue (6-10 mg) was placed in 3 ml of ice-cold nuclei extraction buffer (NEB) [0.32 M sucrose ...
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bioRxiv - Molecular Biology 2021Quote: ... for 6 h at 37°C in the absence or presence of RNase H or RNase A (40 U, NEB). Digested nucleic acids were cleaned with phenol-chloroform extraction and resuspended in nuclease-free water ...
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bioRxiv - Biochemistry 2021Quote: ... 1 μg of total RNA was depleted of rRNA using NEBNext rRNA Depletion Kit (Human/Mouse/Rat) prior to cDNA synthesis primed with random hexanucleotide oligonucleotides (Random Primer 6, NEB). Sequencing library construction was carried out using NEBNext Ultra II FS DNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Genomics 2020Quote: ... and half of the DNA was 3’-end labeled for 1 h at 37 °C in a 10-μl reaction containing 6 units of terminal deoxynucleotidyl transferase (New England Biolabs), 0.25 mM CoCl2 ...