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Citations for New England Biolabs :
1401 - 1450 of 7560 citations for 2' Chloro 4 5 5 dimethyl 1 3 dioxan 2 yl butyrophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... 5% v/v PNGaseF (New England Biolabs, Ipswich, MA, USA) and 50 mM NEM were added to lysates mixed with 1 × GlycoBuffer and incubated on ice for 1 h ...
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Glucocorticoid receptor collaborates with pioneer factors and AP-1 to execute genome-wide regulationbioRxiv - Genomics 2021Quote: ... The 5′ end was repaired by decapping with RppH (NEB) for 1 hour at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 5 μg/ml ScFv16 and 25 mU/ml apyrase (NEB). The solubilisation was incubated stirring at 4 °C for 2 hours before insoluble material was removed by centrifugation at 30,000g for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 5 μg/ml NB35 and 25 mU/ml apyrase (NEB). The solubilisation was incubated stirring at °C for 2 hours before insoluble material was removed by centrifugation at 30,000g for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and NEB® 5-alpha F’IQ (New England Biolabs, NEB) were used for routine cloning procedures and chemically competent E ...
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bioRxiv - Molecular Biology 2021Quote: ... and NEB® 5-alpha F’IQ (New England Biolabs, NEB) were used for routine cloning procedures and chemically competent E ...
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bioRxiv - Evolutionary Biology 2022Quote: ... coli in 5 x 25 uL cell aliquots (NEB #C3020). Each cell aliquot was recovered in 1 mL outgrowth media at 37°C for 1 hr and then transferred into 100 mL of molten LB (1% tryptone ...
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bioRxiv - Genomics 2023Quote: ... The library was transformed into 5-alpha electrocompetent cells (NEB), grown in liquid culture ...
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bioRxiv - Genomics 2023Quote: ... The libraries were transformed into 5-alpha electrocompetent cells (NEB). The plasmid library was prepared by maxi-prep (Sigma) ...
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bioRxiv - Molecular Biology 2024Quote: ... The 5’-phosphate was added via T4 polynucleotide kinase (NEB) at 37 ºC for 30 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Protruding 5’ ends were filled in with Klenow enzyme (NEB) in the presence of [P]-32 dCTP (Hartmann Analytics ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg of replicon plasmid was linearised with AscI (NEB), purified by phenol-chloroform extraction ...
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bioRxiv - Genomics 2024Quote: ... The 5′ adapter was ligated with T4 RNA ligase (NEB), followed by a third biotinylated RNA enrichment ...
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bioRxiv - Bioengineering 2024Quote: ... grown in 5-alpha competent Escherichia coli (NEB, Ipswich, MA) and purified using the PureYield MidiPrep System (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was mixed with 5 U of T4 PNK (NEB), DNA splint (0.4 pmol ...
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bioRxiv - Synthetic Biology 2022Quote: ... Assembled products were heat-shock transformed to 5-alpha (NEB), TOP10 (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... 5 µL 10x T4 DNA ligase reaction buffer (NEB, #B0202S), 2 µL 400U/µL T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’P was created by T4 PNK treatment (NEB, M0201S), done according to the NEB T4 PNK protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 µL 10x Standard Taq Reaction Buffer (New England Biolabs) and 0.25 µL Taq-DNA polymerase (1.25 units/50 µL reaction) ...
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bioRxiv - Plant Biology 2023Quote: ... 5% (v/v) BsaI at 20 U/μL (NEB #R3733), 10% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was then applied to 5 mL chitin resin (NEB) prewashed with HEGX buffer and incubated for 1 hr at 4 °C with agitation ...
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bioRxiv - Cell Biology 2023Quote: ... NEB 5-alpha competent E.coli (NEB #C2987H, Ipswich, MA, USA) were transformed with ligated plasmid by heat shock at 42°C for 30 seconds ...
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bioRxiv - Cancer Biology 2023Quote: ... Linker reactions were removed with 5′ deadenylase (New England Biolabs) and Rec J Exonuclease (NEB) ...
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bioRxiv - Biochemistry 2023Quote: ... Assembled plasmids were transformed into NEB 5-alpha F’Iq (NEB) and standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5′-hydroxyl group repair (T4 polynucleotide kinase (M0201L; NEB) were conducted on beads ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 μL of T4 PNK buffer (NEB, cat. no. M0201S) and 5 μL of 10 mM ATP (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... and 5 µl of prestained protein ladder (New England Biolabs). The gel was electrophoresed at 150 V ...
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bioRxiv - Genomics 2024Quote: ... and dTTP with 5 U/µL DNA polymerase (NEB # M0210), the DNA was ligated by T4 DNA ligase (NEB # M0202 ...
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bioRxiv - Physiology 2024Quote: ... 5 μL of α1-2,4,6 fucosidase O (2U/μl, NEB) with 1x GlycoBuffer 1 (NEB).
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bioRxiv - Plant Biology 2024Quote: ... 5 and 7dpi using a total RNA extraction kit (NEB) and following the kit instructions ...
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bioRxiv - Microbiology 2024Quote: ... 5 μl inorganic pyrophosphate (New England Biolabs product number M2403), and ultrapure water (as needed ...
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bioRxiv - Molecular Biology 2024Quote: ... Five μl of 5 U/μl Klenow fragment (NEB M0210L) was added to achieve a final concentration of 0.5 U/μl and incubated at 37°C for 15 min with the same agitation program as the previous step ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 μL T4 Polynucleotide Kinase Reaction Buffer (NEB, Cat #B0201S) and 5 μL 10 mM ATP ...
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bioRxiv - Immunology 2024Quote: ... and 5 μL of Proteinase K (NEB catalog no. P8107S). Tubes were incubated for 15 min at 37 °C with shaking at 600 rpm ...
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bioRxiv - Bioengineering 2024Quote: ... 5’-end labeling was performed using T4 Polynucleotide Kinase (NEB) and [gamma-32P] ATP (PerkinElmer) ...
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bioRxiv - Genomics 2024Quote: ... and 5 µl DNA Polymerase I (Large Klenow Fragment) (NEB). The reaction was incubated for 60 min at 37 °C and subsequently purified and eluted in 13 µl ddH2O using a MinElute kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: Chemically competent NEB® 5-alpha F’Iq cells (NEB; C2992H) were transformed with recombinant plasmids per manufacturer instructions and grown at 37°C on selective Luria-Bertani (LB ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of T4 DNA ligase (NEB, 400000 U/mL), and 10 μL of 10X T4 DNA ligase buffer (NEB) ...
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bioRxiv - Bioengineering 2024Quote: ... then 5 μL of 6X Purple Loading Dye (NEB, B7024S) were added and run on a GelRed-stained 1% (w/v ...
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bioRxiv - Genomics 2022Quote: ... 2 μl of 10X CutSmart® buffer (1X final) and 2 μl of 1U/μl USER® II enzyme (M5505S, NEB) (0.1U/μl final ...
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bioRxiv - Microbiology 2020Quote: ... stained with a mAb cocktail composed of SARS-CoV-2 spike (Creative-Bios; 2BCE5) and SARS-CoV-2 nucleoprotein (Creative-Biolabs; NP1C7C7) followed by anti-Mouse IgG-HRP (Abcam ab6823 ...
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bioRxiv - Genomics 2021Quote: ... and supernatant was transferred to a new tube and added to 2 μL 10% SDS and 2 μL 20 mg/mL proteinase K (New England Biolabs P8107). Samples were incubated overnight at 65°C to reverse crosslinks ...
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bioRxiv - Genetics 2023Quote: ... 5 µl of 100 ng/µLcDNA diluted from the previous step was combined with 2 µL of block mix and 2 µL of nuclease free water (NEB, AM9937), and then the cDNA block oligo mix was incubated on a thermocycler under the following conditions to allow block oligo mix to bind to the 5′ end and the 3′ end of the cDNA molecule ...
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bioRxiv - Microbiology 2023Quote: ... antibiotic resistance cassette and 2 flanking regions were combined in equimolar amounts and mixed with 2 x HiFi reagent (NEB, UK) and incubated at 50°C for 1 hour ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 1×Buffer 4 (NEB). The reaction was stopped (6 mM EGTA ...
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bioRxiv - Genomics 2022Quote: ... 7 μl PEG 8000 (17.5% final) and 1 μl of T4 RNA ligase 2 KQ (NEB, M0373L) in 1X T4 RNA ligase buffer ...
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bioRxiv - Biochemistry 2020Quote: 1 μg of N protein was dissolved in 36 μL H218O and 2 μL 10x glycobuffer (NEB). To this solution 2 μL PNGase F was added and the reaction mixture was incubated at 37 °C for 16 h ...
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bioRxiv - Genomics 2021Quote: ... and NEBNext® Multiplex Oligos for Illumina® Index Primers Set 1 and 2 (NEB #E7335S, #E7500S), following the manufacturer’s instructions with minor modifications ...
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bioRxiv - Molecular Biology 2022Quote: ... All enzymatic reactions were performed as a 1-step reaction with 1x Glycobuffer 2 (New England Biolabs), 10 μg RBD produced in CHO-S- ...
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bioRxiv - Molecular Biology 2023Quote: ... The samples were multiplexed with NEBNext Multiplex Oligos for Illumina (Index Primers Set 1 and 2) (NEB). Size selection steps were performed with Ampure XP beads (Beckman Coulter ...