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Citations for New England Biolabs :
1401 - 1450 of 3709 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... followed by overnight treatment with apyrase (1-unit, NEB) on ice ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μL of 20 mg/mL Proteinase K (NEB), and 1 μL of 10 mg/mL RNase A (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 μL recombinant shrimp alkaline phosphatase (NEB cat#M0371S) was added to the reaction and incubated at 37°C for 1hr ...
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bioRxiv - Molecular Biology 2024Quote: ... Gaussia princeps luciferase (New England Biolabs, #E8023, 1:5,000), Histone H3 (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2024Quote: ... rabbit anti-cleaved caspase-3 1:100 (9661, NEB), rat anti-p21 1:100 (ab107099 ...
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bioRxiv - Physiology 2024Quote: ... 1 μl 10 mg/ml Proteinase K (Biolabs, #P8107S), 1 μl 10 mg/ml RNase A plus 4.5 μl 5 M NaCl ...
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bioRxiv - Genomics 2024Quote: ... 1% v/v murine RNase inhibitor (New England Biolabs), 0.1% w/v yeast tRNA ...
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bioRxiv - Synthetic Biology 2024Quote: ... The DNA 1 kb+ ladder (New England Biolabs, N3200) was used as a reference ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:20 diluted oligos were ligated (T4 ligase NEB) into 50ng of cut and purified vector then transformed into bacteria ...
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bioRxiv - Biochemistry 2024Quote: ... for 1 h or 10 units of PstI (NEB) for the indicated time points ...
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bioRxiv - Genomics 2024Quote: ... 5 µl 10x NEBuffer2 and 1 µl M.SssI (NEB) in a total volume of 50 µl (topped off with ddH2O) ...
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bioRxiv - Genomics 2024Quote: ... 1 µM NEBNext Universal PCR Primer for Illumina (NEB) was added to each reaction followed by amplification at 98°C for 45 sec ...
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bioRxiv - Bioengineering 2024Quote: ... followed by addition of 1 μL DpnI (NEB, R0176S) and incubation at 37°C for 1 hour and 80°C for 20 min) ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 1 X CutSmart buffer (New England Biolabs) and 6 μL Quick CIP (New England Biolabs) ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 1 uL of Murine RNase inhibitor (NEB) and 125 ng of the probe plasmid DNA as per the kit protocol ...
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bioRxiv - Cell Biology 2024Quote: ... anti-GFP from rabbit (1:1000, Torrey Pine Biolabs). Secondary antibodies from mouse and rabbit were respectively coupled to Alexa-647(1:200 ...
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bioRxiv - Microbiology 2024Quote: ... 1 µl of Proteinase K (800 units/ml, NEB) was added ...
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bioRxiv - Evolutionary Biology 2024Quote: ... in 1X NEB buffer 1 (New England Biolabs B7001) (30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... resuspended in 1× ThermPol buffer (New England BioLabs B9004S) + 200 µg/ml bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2024Quote: ... 1 μg of PacI (New England Biolabs, Ipswich, MA) linearized recombinant adenovirus plasmid was transfected to HEK293AD cells using Lipofectamine 2000 (ThermoFisher ...
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bioRxiv - Systems Biology 2022Quote: ... 5’-AAAC(N)19-20-3’) by combining 1 μl of each 100 μM oligonucleotide with 1 μl of 10× T4 ligation buffer (NEB cat. no. B0202S), 6.5 μl of water ...
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bioRxiv - Genomics 2020Quote: ... 50-100 fmol of PCR products were diluted in 9 µl then mixed with 1 µl of Rapid 1D Adapter and 1 µl of Ligase T4 Blunt (New England Biolabs, Ipswich, MA, USA). Pre-sequencing mix was incubated 10 min at 25°C and left on ice until ready to load ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment release step was performed in 5 μl 1% SDS supplemented with 1:10 Thermolabile Proteinase K (New England Biolabs cat. no. P8111S) at 37°C 1 hr followed by 58°C 1 hr ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragment release step was performed in 5 μl 1% SDS supplemented with 1:10 Thermolabile Proteinase K (New England Biolabs cat. no. P8111S) at 37°C 1 hr followed by 58°C 1 hr ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 µM of the 6-kb fragment was incubated with 1 unit/µL terminal deoxynucleotidyl transferase (TdT, New England Biolabs, MA, USA, #M0315S) and 0.5 mM deoxyadenosine triphosphate (dATP ...
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bioRxiv - Immunology 2023Quote: ... We then used a 2:1 insert to vector ratio in a 1 hour Hifi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). The Hifi assembly products were Ampure bead purified and eluted into 20 µL of H2O ...
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bioRxiv - Microbiology 2023Quote: ... We then used a 2:1 insert to vector ratio in a 1 hour HiFi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). HiFi assembly products were Ampure bead purified and eluted into 20uL of H2O for higher electroporation efficiency.
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bioRxiv - Microbiology 2022Quote: ... We then used 1:3 insert to vector ratio in a 1 hour Hifi assembly reaction using NEBuilder HiFi DNA Assembly kit (NEB, Cat. No. E2621). After the HiFi assembly ...
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bioRxiv - Bioengineering 2023Quote: ErCas12a (1 μg) or LbCas12aU (1 μg) protein and 1 μg crRNA were assembled in 1X Nuclease Reaction Buffer (New England BioLabs, Ipswich, MA, USA) at room temperature for 10 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Each reaction was performed in a 10 μL volume containing 1× Luna Universal One-Step Reaction Mix plus 1× Luna WarmStart® RT Enzyme Mix (Cat. No. E3005; New England BioLabs® Inc.), 0.4 µM of each primer and 2 µL of 10 ng/µL RNA ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was mixed with Reaction Mix 1 (9:1 of 12 uM NEB RT random 6N-S to 12 uM oligo-dT(20), 12uM dNTPs ...
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bioRxiv - Synthetic Biology 2021Quote: ... 5 µg of empty vector and 1 µg of purified amplicon were digested overnight with 1 µl of SphI-HF® (New England Biolabs, catalog no. R3182) in a total volume of 50 µl ...
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bioRxiv - Bioengineering 2022Quote: ... and 15ul BseYI-digested product were run side-by-side on gel (1% agarose gel in TAE buffer, 1 kb ladder (NEB, Cat. No. / ID: N3232L), most gels run at 115V for 40 minutes) ...
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bioRxiv - Genomics 2023Quote: ... Lysed cells were then diluted 10 time in several tubes containing 450 μl of digestion mix (1X NEB 1 buffer, 1% Triton X-100). 100 units of HpaII were added and the tubes were incubated for 2 hours at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with mouse anti-MBP (NEB, 1:500), followed by washing and subsequent incubation with goat anti-mouse IgG ...
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bioRxiv - Genetics 2021Quote: ... T7 endonuclease 1 assay was performed following manufacturer’s protocol (NEB).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were stained with mouse anti-MBP (NEB, 1:500), followed by washing and subsequent incubation with goat anti-mouse IgG ...
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bioRxiv - Developmental Biology 2021Quote: ... and mounted in 1% low melting point agarose (LMP) (Biolabs) within a 1mm diameter glass capillary ...
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bioRxiv - Genomics 2020Quote: ... 1 μl (10 U/μl) of T4 polynucleotide kinase (NEB) and 1 μl of 10 mM dNTPs in a final volume of 50 μl at 24°C for 30 min ...
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bioRxiv - Genomics 2020Quote: ... 1 μl (3 U/μl) of T4 DNA polymerase (NEB), 1 μl (10 U/μl ...
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bioRxiv - Physiology 2022Quote: ... anti-MBP (1:10,000, catalog no. E8032S, New England Biolabs); anti-CCNA2 (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... Excess adapter was digested by adding 1 µl RecJf (NEB) and 1 µl 5’deadenylase (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... both proteins were deglycosylated by PNGase F (NEB, 1:50) overnight at 37°C in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-GFP (1:1000; TP401, Torrey Pine Biolabs, RRID:AB_10013661), mouse anti-GFP (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... and 1 unit of poly(A) polymerase (New England Biolabs) and was incubated at 42°C for 1 hour followed by enzyme inactivation at 95°C for 5 minutes ...
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bioRxiv - Genomics 2020Quote: ... 5′ adapter ligation with T4 RNA Ligase 1 (NEB, M0204L). The resulting RNA fragments were used for a reverse transcription reaction using Superscript III Reverse Transcriptase (Life Technologies ...
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bioRxiv - Genomics 2020Quote: ... 0.1mM dNTPs and 1 U/μl USER enzyme (NEB, USA). 75μl of the mix was added and incubated for 3h at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... 1× Phusion HF buffer (New England Biolabs GmbH, Schwalbach, Germany) with 1.5 mM MgCl2 ...
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bioRxiv - Genomics 2021Quote: ... and 1 µl of PNK (NEB M0201, 10,000 units/ml) and incubated at 37 °C for 20 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... were ligated using 1 μl Quick T4 DNA Ligase (NEB) and DNA fragments were amplified by PCR with NEXTFlex primers (Primer 1 - 5’-AATGATACGGCGACCACCGAGATCTACAC ...