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Citations for New England Biolabs :
1351 - 1400 of 4157 citations for Rabbit Caveolin 1 CAV1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... original numbering with signal sequence = 20–559) were subcloned into the manufacturer-supplied plasmid from the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, Ipswich, MA, USA) using the Gibson assembly method with synthetic E ...
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bioRxiv - Microbiology 2021Quote: ... MNase activity was quenched by addition of EGTA and samples were subsequently pre-cleared by addition of 40 µl Protein A Magnetic Beads (New England Biolabs, Ipswich, MA, United States) and rotating at 4°C for 60 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Removal of both N-linked and O-linked glycans was performed by incubation with five different glycosidases: PNGaseF (New England Biolabs, 125 U/μg protein), O-glycosidase (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... aliquots of the same protein extract were separated and treated with 200 U of Lambda Phage Phosphatase (#P0753S, New England Biolabs, Ipswich, Massachusetts, United States), as specified by the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: Injections into fertilized eggs for knockout experiments were done using 50ng/µl of each sgRNA independently and 80nM of NEB EnGen Spy Cas9-NLS protein (New England Biolabs France Genopole, Evry, France), pre-incubated at room-temperature for 15 minutes (Hill et al. ...
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bioRxiv - Plant Biology 2024Quote: ... when OD600 reached 0.5 for 4 h at 37 °C and then they were purified using amylose resin column (New England Biolabs, for MBP related proteins purification) or Ni-NTA agarose column (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... 1 mM ATP and 10 mM DTT and with 1 μl (2 U/μl) of RNase H (NEB), 1 μl (3 U/μl ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA was diluted to 0.2 ng.μl-1 and 1 ng was analysed by qPCR using Luna Universal qPCR MasterMix (NEB) with 500nM of primers ...
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bioRxiv - Molecular Biology 2020Quote: ... Methylated adapters (RRBS-1, RRBS-2; Table 1) were added by ligation using DNA ligase (New England Biolabs). DNA was purified using ratio of 1:1.2 sample to AMPure XP beads ...
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bioRxiv - Genomics 2020Quote: ... before and in between the following procedures: (1) 3′ adapter ligation with T4 RNA Ligase 1 (NEB, M0204L), (2 ...
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bioRxiv - Cell Biology 2020Quote: ... Single-stranded DNA primers and primer dimers were digested using 1 unit μL−1 ExoI (New England Biolabs) and 1 U μL−1 HinFI (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... but instead of ATP 1 mM MnCl2 was added and 1 μl (400 U) of λ phosphatase (NEB) was used instead of PKR.
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bioRxiv - Cell Biology 2021Quote: ... with additionally 1 μl oligo-dT primers and 1 μl of dNTP mix (NEB Biolabs, Ipswich, MA, USA). Further ...
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bioRxiv - Cell Biology 2021Quote: ... with additionally 1 μl oligo-dT primers and 1 μl of dNTP mix (NEB Biolabs, Ipswich, MA, USA). Further ...
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bioRxiv - Microbiology 2022Quote: ... RNA integrity was confirmed by mixing samples 1:1 in 2X RNA loading dye (B0363S; New England Biolabs) and separating by 0.8 % (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... or anti-MBP antibody (1:10.000, used with 1:10.000 secondary horseradish peroxidase-conjugated anti-mouse antibody; NEB).
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bioRxiv - Bioengineering 2021Quote: ... 1:15 and 1:30 (PEI-AuNCs:siRNA)) were incubated with RNase H (10μg/ml, New England BioLabs, M0297L) at 37°C for 30 minutes ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Each reaction was visualised on a 1 % agarose gel using a 1 kb DNA ladder (New England Biolabs) and a Lambda ladder (Roche ...
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bioRxiv - Molecular Biology 2022Quote: ... The products were resolved in 1% agarose gels along with 1 kb Plus DNA Ladder (New England Biolabs) and visualized using SYBR™ Safe DNA Gel Stain (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... 1 µl 10x BSA (diluted 1:10 with Milli-Q water from a 100X stock, New England Biolabs), and 1 µl of each enzyme used as described in Fig ...
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bioRxiv - Neuroscience 2023Quote: ... column purified and ligated at a 1:1 vector to insert ratio using the QuickLig Kit (NEB #M2200S). The ASCL1 stop codon was subsequently mutated using the QuickChange II-XL site-directed mutagenesis kit (Agilent #200523 ...
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bioRxiv - Microbiology 2024Quote: ... 5 mM MgCl2) and either 25 nM NudE.1 WT or NudE.1 E64,65Q or NudC WT (NEB). For NAD spike-in kinetics ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 µl 100× Protease inhibitor cocktail and 1 µl of CviKI-1 (5 U/100,000 nuclei, NEB R0710S) were added ...
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bioRxiv - Plant Biology 2024Quote: ... we used a 1:1 mix of Low Range ssRNA Ladder (New England Biolabs™, catalog no. N0364S) and 14-30 nt ssRNA Ladder Marker (Takara™ ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μl ligation enhancer (New England BioLabs), 0.9 μl nuclease-free water and 0.1 μl duplexed adapter (100 μM ...
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bioRxiv - Cell Biology 2020Quote: ... Index Primers Set 1 (New England Biolabs). P5/P7 PCR fragments were purified with Agencourt AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Cell Biology 2020Quote: ... T7 Endonuclease-1 was purchased from NEB, US (Cat ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 1 uL of PNK enzyme (NEB M0236S), and 2 uL of 1mM ATP to total 10 uL and incubated at 37 C for 30 minutes followed by inactivation by heating to 65 C for 20 minutes.
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bioRxiv - Developmental Biology 2021Quote: ... and 1 U of Klenow fragment (NEB) at 20°C for 30 minutes ...
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bioRxiv - Genomics 2020Quote: ... 1 μl of NEBNext Ligation Enhancer (NEB), and 2 μl of 1.5 μM NEBNext adaptors for Illumina sequencing (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... and 1 µl of PNK enzyme (NEB), carried out at 37°C for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... 1 µl of 10x PNK buffer (NEB), 1 µl of rSAP enzyme (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 unit/µl Murine RNase Inhibitor (NEB), protease inhibitor (Pierce ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 unit/µl Murine RNase Inhibitor (NEB), protease inhibitor (Pierce ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 unit/µl Murine RNase Inhibitor (NEB), protease inhibitor (Pierce ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 unit/µl Murine RNase Inhibitor (NEB), protease inhibitor (Pierce ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 µL Klenow large fragment (NEB) in 100 µL T4 DNA ligase buffer with 0.25 mM dNTPs for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: Using T4 RNA ligase 1 (NEB, M0437M), total RNA was isolated from 10-week-old or senescent rosettes of WT plants were ligated to a specific oligo adaptor at their 5’terminal ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse monoclonal anti-MBP (1:2000, NEB), rabbit polyclonal anti-Spo11 (1:1000 ...
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bioRxiv - Genomics 2022Quote: ... and 1 μl 5’deadenylase (NEB, M0331) into the 20 μl ligation reaction for incubation with 30 minutes at 30°C and 3 minutes at 65°C to inactivate the enzymes followed by column purification (Zymo Research ...
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bioRxiv - Genomics 2022Quote: ... and 1 μl PNK enzyme (NEB, M0201S) in 1X T4 PNK buffer ...
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bioRxiv - Microbiology 2020Quote: ... HiFi Polymerase (1 unit of Phusion, NEB) in GC buffer (containing MgCl2 ...
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Genetic mapping of fitness determinants across the malaria parasite Plasmodium falciparum life cyclebioRxiv - Evolutionary Biology 2019Quote: ... 1× Phi29 reaction buffer (New England Biolabs), and 30 units of Phi29 polymerase (New England Biolabs) ...
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bioRxiv - Cancer Biology 2020Quote: ... and 1 μl BglII (New England Biolabs). The reaction mixture was incubated for 1 hour at 37°C and then ran on a 1.5% Agarose gel.
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bioRxiv - Molecular Biology 2020Quote: ... 1 mM rNTP mix (New England Biolabs), 0.75 μL T7 polymerase (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 µL 10x T4 ligase buffer (NEB), 0.5 µL mg/mL bovine serum albumin (NEB) ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 mg/mL bovine serum albumin (NEB): 25 nL ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1 mg / mL bovine serum albumin (NEB): 25 nL ...
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bioRxiv - Genomics 2020Quote: ... and 1 µL T4 DNA Ligase (NEB) in a 10 µL reaction (filled up to total volume with H2O) ...
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bioRxiv - Biophysics 2019Quote: ... 1 U/µL murine RNase inhibitor (NEB), 200 ng mRNA ...