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Citations for New England Biolabs :
1351 - 1400 of 10000+ citations for Cortisone Chemiluminescent ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... sufficient colonies were scraped from agar plates for >400-fold library coverage and DNA was extracted (T1010L, New England Biolabs). DNA concentrations were measured using a NanoPhotometer N60 (Implen).
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bioRxiv - Cell Biology 2019Quote: ... HeLa-CENP-A-SNAP cells were grown in 6 well plates and pulse labeled with tetra-methyl-rhodamine-conjugated SNAP substrate (TMR-Star; New England Biolabs) at 4µM final concentration ...
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bioRxiv - Cancer Biology 2020Quote: ... were added to each quadrant of the 96-well plate (n = 24 unique adapters) with a ligation mixture of 40 Weiss U T4 ligase (NEB), 1mM ATP (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Single antigen specific memory B cells were sorted on BD FACS Aria II into 96-well PCR plates (Axygen) containing 10 μl per well of lysis buffer (10 mM DPBS, 4 U Mouse RNase Inhibitor, NEB). Plates were immediately frozen on dry ice and stored at 80 C or processed for cDNA synthesis.
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bioRxiv - Molecular Biology 2022Quote: ... Ninety-six 12 μl Gibson reactions were performed in 96-well PCR plates using Gibson Assembly Master Mix (NEB E2611L). The Gibson reaction mix was transformed into Mach1 competent cells (4 μl Gibson into 40 μl cells ...
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bioRxiv - Genomics 2022Quote: ... Oligomers in each well of a 384-well plate were amplified with Q5 hot-start DNA polymerase (New England Biolabs) using primers SAC615 and SAC576 ...
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bioRxiv - Genomics 2024Quote: ... Resulting dsDNA was purified using a 1X SPRIbead clean-up within the 96-well plate, and the resulting product was subjected to USER digestion (80% ddH2O, 10% 10X rCutsmart, 10% USER enzyme (NEB)) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells and streaked onto Luria-Burtani (LB) plates containing carbenicillin. All-by-all repressor constructs (Fig. 5c) were cloned by digestion with BsiWI-HF (NEB) and BbsI (NEB) ...
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bioRxiv - Neuroscience 2023Quote: ... 3 µL of RNA from 4 x 96 well plates was transferred to a 384 well plate with 3 µL of master mix containing 1 µL of a 10 mM stock of dNTPs (NEB Catalog# N0447L ...
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bioRxiv - Cancer Biology 2022Quote: ... reactions were performed in 96-well plates using 5ng of cDNA samples using the Luna Universal qPCR Master Mix (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... of each sample was transferred to and digested in a 96-well plate with the restriction enzyme SbfI-HF (NEB). Custom P1 adapters (Hohenlohe et al ...
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bioRxiv - Biophysics 2021Quote: ... 1 μL of this PCR reaction was then combined with 5 μL Q5 buffer (NEB, cat#M0491S), 0.5 μL 10mM DNTP (Thermo Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The annealed product was labeled by fill-in synthesis using 5 U Klenow exo-(New England Biolabs) and a dNTP mix containing 40 µM each of dATP ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were incubated with 2 nM HaloTag-JF549 and 5 nM SNAP-Cell® 647-SiR (NEB) before imaging.
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bioRxiv - Molecular Biology 2021Quote: ... A preadenylated universal linker (5’-rAppCTGTAGGCACCATCAAT-NH2-3’) was prepared in house or purchased from NEB (S1315S) and ligated to the 3’ ends of the dephosphorylated footprints using T4 RNA Ligase 2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then incubated at 45 °C for 2 hr with 5 μL of Proteinase K (NEB) in the presence of 40 mM EDTA (pH 8.0 ...
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bioRxiv - Biophysics 2022Quote: ... The donor-labeled DNA was phosphorylated at the 5’ end using T4 Polynucleotide Kinase (New England BioLabs) for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... The purified and desalted ligation product was then electroporated into high efficiency 5-alpha E.coli cells (NEB). The cells were grown overnight on LB-Carbenicillin plates and then ...
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bioRxiv - Molecular Biology 2019Quote: ... Subsequently 5’ and 3’ termini were ligated using 10 units of T4 RNA ligase (New England Biolabs) at 37°C for 1 h ...
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bioRxiv - Genomics 2021Quote: ... 50 ng of DNA was digested using the 5-bp cutter ApeKI (New England Biolabs, Ipswich, MA) after which barcoded adapters were added by ligation with T4 ligase (New England Biolabs ...
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bioRxiv - Genetics 2020Quote: ... We mixed 20 µg of insert or 15 µg plasmid library with 5 µL AscI (NEB R0558L), 5 µL SbfI-HF (NEB R3642L) ...
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Differential impact of a dyskeratosis congenita mutation in TPP1 on mouse hematopoiesis and germlinebioRxiv - Cell Biology 2021Quote: ... 5′ 32P-labeled (TTAGGG)4 oligonucleotide (labeled using [γ-32P]ATP and T4 PNK; New England Biolabs) was added ...
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bioRxiv - Bioengineering 2021Quote: ... 5′ phosphorylation was performed either during synthesis (for tRNAfMet) or following synthesis using T4 Polynucleotide kinase (NEB)(for tRNALys and tRNAPhe ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: GBL phosphate (8) was incubated with 5 units of calf intestinal alkaline phosphatase (CIP, New England Biolabs) in 50 mM HEPES buffer (pH 8.0 ...
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bioRxiv - Cell Biology 2021Quote: ... FHIP1B or FHIP2A-coated IgG beads were labeled with 5 µM HaloTag-Alexa-660 (New England Biolabs) in the column for 10 minutes at room temperature and unbound dye was removed with a 300 mL wash with TEV buffer at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... Reactions conducted at UR-MAI consisted of 50 μl reactions: 5 μl 10X Standard Taq Buffer (NEB), 2.5 μL of each primer ...
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bioRxiv - Microbiology 2020Quote: ... and then added up to 5 fmol DNA (2000:1000:1 RNA:protein:DNA ratio) and NEBuffer 3.1 (NEB) to 1X ...
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m7G cap-eIF4E interaction stimulates polysome formation by enhancing first-round initiation kineticsbioRxiv - Biophysics 2021Quote: ... 5’-end capping and 3’-end biotinylation were performed using the Vaccinia Capping System (New England Biolabs) and the Pierce RNA 3’ End Biotinylation Kit (Thermo Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... 3 µl Antarctic phosphatase [5 U] and 7.32 µl Antarctic phosphatase buffer (both New England BioLabs, Germany). The reaction was heat-inactivated at 85°C during 15 min ...
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bioRxiv - Biophysics 2020Quote: ... was added to the flow cell and incubated for 5 minutes followed by Nb.BsmI (0.2 U/μl) in 1x NEB 3.1 buffer (NEB) which was also incubated for 5 minutes ...
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bioRxiv - Genomics 2021Quote: ... The cell suspension was then mixed with 5 ml ligation buffer (550 μl 10x ligation buffer (NEB), 27.5 μl 20 mg/ml BSA (NEB ...
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bioRxiv - Biochemistry 2022Quote: ... Finale expression constructs were subcloned in NEB 5- alpha competent E.coli cells (New England Biolabs, Ipswich, US) followed by Sanger sequencing (Microsynth ...
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bioRxiv - Microbiology 2022Quote: ... as follows: 1µL of 2µM MBTUni-12 primer (5’-ACGCGTGATCAGCRAAAGCAGG-3’) + 1µL 10mM dNTPs Mix (NEB #N0447S) + 8µL Nuclease-free water (Ambion ...
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bioRxiv - Biochemistry 2022Quote: ... mixing with 5 μL of stop buffer (50 mM EDTA and 2 mg/ml Proteinase K (NEB)) ...
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bioRxiv - Microbiology 2022Quote: ... 23 nt RNA (5’-GAAUCUAUACAUAAAGACCAGGC-3’) was capped with vaccinia capping enzyme and 2’-O-methyltransferase (NEB) and radiolabelled with [α 32P]-GTP ...
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bioRxiv - Neuroscience 2022Quote: ... 45 nM pegRNA3-3AGTGA (Appendix Table S1) and 5 µl NEBuilder HiFi DNA Assembly Master Mix (NEB) and incubated at 37°C for 1 h.
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bioRxiv - Biochemistry 2020Quote: ... Fragmentation of 1 µg mRNA aliquots for 5 min at 94°C in 1x Fragmentation Buffer (NEB) was optimal to obtain a pool of 100-200 nt fragments ...
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bioRxiv - Microbiology 2022Quote: ... with the 5’ monophosphates available on the treated viral RNAs using T4 RNA ligase (NEB, Ipswich, MA) for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... to digest the bottom strand of dsDNA and truncated the 5’ primer region with USER enzyme (NEB). We added 5 M NaCl 20% v/v to the oligo mix and pre-annealed by heating it at 95°C for 3 minutes ...
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bioRxiv - Biophysics 2022Quote: ... a third primer ACTB K118TAG phosphorylated at its 5’ end and Taq DNA ligase (New England Biolabs) were added to the reaction mixture ...
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bioRxiv - Neuroscience 2019Quote: ... and used to transform NEB 5-α competent Escherichia coli cells (New England Biolabs, Whitby, ON, Canada). After screening plasmid constructs for directionality using T7 promoter oligonucleotide and gene-specific primers (Table S1) ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA fragments were directly 3’-end dephosphorylated using 5 U of Antarctic Phosphatase (New England Biolabs, UK) for 30 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... 5 μL of plasma was lyophilized and resuspended in 20 μL 1X Rapid PNGaseF buffer (NEB #P0710S) and incubated for 15 minutes at 70°C to denature proteins ...
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bioRxiv - Cell Biology 2020Quote: ... An 8.9 kb fragment preceding and including exon 5 was amplified by PCR (Phusion DNA Polymerase, NEB) and cloned into a plasmid encoding both a floxed neomycin resistance cassette and a diphtheria toxin negative selection marker ...
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bioRxiv - Microbiology 2019Quote: Second-strand extension was performed using Klenow 3’ → 5’ exo− fragment (New England BioLabs, Ipswich, MA USA). Double-stranded cDNA was amplified using AmpliTaq Gold polymerase (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... 10X T4 DNA ligase buffer (5 uL) and T4 DNA ligase (320 units; M0202S, New England Biolabs) and incubating at room temperature for 3 hours and then 65 °C for 10 minutes.
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bioRxiv - Molecular Biology 2021Quote: ... DNA guides used for cleavage (Supplementary Table S3) were 5’-phosphorylated using T4 PNK (New England Biolabs) except when cleavage with 5’-OH guides ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA pellets were resuspended in 5 µL dephosphorylation reaction mix (7 mM Tris pH 8, 1x NEB T4 PNK buffer ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL of 5 μM annealed adaptors and 10 μL of Blunt/TA ligase master mix (NEB) was added to each reaction ...
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bioRxiv - Microbiology 2021Quote: ... and pvhsp70 5’ UTRs were digested using BlpI and SpeI restriction enzymes (New England Biolabs, Ipswich, MA), and agarose gel-purified fragments were subcloned into the plasmid pD-pfcam-Luc also digested with these enzymes ...