Labshake search
Citations for New England Biolabs :
1351 - 1400 of 4162 citations for 2 4 Methyl 5 thiazolyl ethyl decanoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: 5’ end phosphorylation (1x PNK buffer, 20 u T4 PNK (NEB), 80 u RNaseOUT ...
-
bioRxiv - Molecular Biology 2022Quote: ... DNA was transformed into NEB 5-alpha cells (New England Biolabs) and plated on LB plates with carbenicillin for selection purposes and incubated overnight at 37 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... followed by 5 µL of PNGase F (New England Biolabs, P0704L). The sample was incubated at 37°C for 3h to allow the de-N-glycosylation to proceed ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and 5 µL of 10x T4 DNA Ligase Buffer (NEB #B0202). The reaction samples were incubated at room temperature for two hours and purified by FastGene PCR/Gel Extraction Kit (Nippon Genetics #FG-91302) ...
-
bioRxiv - Cell Biology 2023Quote: ... Klenow fragment (5 U) & T4 polynucleotide kinase (15 U) (NEB; M0201). The A-tailing was performed with Klenow exo-fragment (15 U ...
-
bioRxiv - Molecular Biology 2023Quote: ... ligated to the 5′ adapter with T4 RNA ligase (NEB M0204L), and reverse transcribed with Superscript III (Thermo Fisher 18080-051) ...
-
bioRxiv - Microbiology 2023Quote: ... with the inclusion of m7G(5’)G RNA Cap Analog (NEB). Electroporation of the resulting RNA was carried out using a Neon® Transfection System (Invitrogen™ ...
-
bioRxiv - Biochemistry 2023Quote: ... The purified DNA was 5’ phosphorylated with T4 Polynucleotide Kinase (NEB) and ligated with homemade T4 DNA ligase ...
-
bioRxiv - Immunology 2022Quote: ... 5 mol% labelled with SNAP-Surface Alexa Fluor 488 (NEB, S9128S), to 25% v/v HeLa lysate in a final volume of 20 µl (50 mM Tris-HCl ...
-
bioRxiv - Genomics 2023Quote: ... RNA was digested with addition of 5 U RNaseH (NEB, M0297) to the first strand cDNA synthesis reaction and incubation at 37 °C for 20 min.
-
bioRxiv - Genomics 2023Quote: ... RNA was digested with addition of 5 U RNaseH (NEB, M0297) to the first strand cDNA synthesis reaction and incubation at 37°C for 20 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... Purified RNA was treated with RNA 5′ pyrophosphohydrolase (New England Biolabs) for 2 h at 37℃ ...
-
bioRxiv - Systems Biology 2023Quote: ... and 5 μL of 400 U/μl T4 DNA ligase (NEB). The ligation reaction was incubated for 4 h at 16°C with occasional inversion of the tube (every 1 h) ...
-
bioRxiv - Genomics 2023Quote: ... 5 μL of 400 U/μL T4 DNA Ligase (NEB #M0202), and incubating at room temperature for ≥4 hours with rotation.
-
bioRxiv - Systems Biology 2023Quote: ... 5 μl of NEB T4 ligase (New England Biolabs, Ipswitch, MA) filled up to a volume of 100 μl with MilliQ H2O (Merck Millipore ...
-
bioRxiv - Systems Biology 2023Quote: ... alongside 5 U of quick alkaline phosphatase (calf intestinal, NEB #M0525S), all in a 30 µl rCutSmart buffer (NEB ...
-
bioRxiv - Molecular Biology 2023Quote: ... and then transformed into 5-alpha competent Escherichia coli cells (NEB), according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2023Quote: ... and 2.5 U Klenow Fragment (3’ -> 5’ exo-) (New England BioLabs) and H2O to 20 μL ...
-
bioRxiv - Microbiology 2023Quote: ... and 5 μM dNTP in 17 μL 1x rCutSmart buffer (NEB). The RNA and primers were then annealed by incubating as follows ...
-
bioRxiv - Molecular Biology 2023Quote: ... 40 μl of 5 pM λ-phage DNA (New England Biolabs), digoxigenin-labelled at both ends14 ...
-
bioRxiv - Neuroscience 2023Quote: ... 5 µL of Colour Prestained Protein Standard (New England Biolabs #P7719S) was included on each gel ...
-
mRNA vaccines and hybrid immunity use different B cell germlines to neutralize Omicron BA.4 and BA.5bioRxiv - Immunology 2022Quote: ... TAP reaction was performed using 5 μL of Q5 polymerase (NEB), 5 μL of GC Enhancer (NEB) ...
-
bioRxiv - Developmental Biology 2022Quote: ... it was transformed into NEB 5-alpha competent E.coli (NEB #C2987). The sequence of donor plasmid was confirmed by Sanger sequencing using 5 primers to ensure that there are no disabling mutations in the scarless DsRed cassette or regions of the homology arms ...
-
bioRxiv - Molecular Biology 2022Quote: ... All guides were 5’-phosphorylated using T4 PNK (New England Biolabs) except for experiments with 5’-OH guides (Fig ...
-
bioRxiv - Biochemistry 2022Quote: ... pH 9.0 and 5 U of Quick CIP (NEB, Ipswich, MA) was added ...
-
bioRxiv - Genetics 2022Quote: ... Isolated sRNA was treated with RNA 5’ Pyrophosphohydrolase (RppH, M0356S, BioLabs), to ensure 5’ monophosphate-independent capturing of small RNAs as previously described [57] ...
-
bioRxiv - Immunology 2022Quote: ... and then transformed in NEB 5-alpha competent cells (NEB, C2987U). Expi293F (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2022Quote: ... The 5′ end was phosphorylated using T4 polynucleotide kinase (NEB, M0201L). A second bead binding was performed ...
-
bioRxiv - Genomics 2022Quote: ... 5′ end phosphorylation using T4 polynucleotide kinase (NEB, Ipswich, MA; M0201), (iv ...
-
bioRxiv - Microbiology 2022Quote: ... and subsequent restriction enzyme assay of exon 5 (with BSaaI, NEB) and exon 7 (BSErI ...
-
bioRxiv - Microbiology 2022Quote: ... coli host strain NEB 5-alpha F’ lq (New England Biolabs) was exclusively used to generate ...
-
bioRxiv - Systems Biology 2024Quote: ... and A-tailed using Klenow HC 3′ → 5′ exo (#M0212L; NEB).
-
bioRxiv - Microbiology 2024Quote: ... Ligation of 5’ linker using T4 RNA ligase 1 (NEB; #M0204S) was conducted after phosphorylating the 5’ end of the pooled fragments with T4 PNK (NEB ...
-
bioRxiv - Molecular Biology 2024Quote: ... dA-tailing was done using Klenow fragment (3′→5′ exo-, NEB) with deoxyadenosine triphosphate (dATP ...
-
bioRxiv - Molecular Biology 2024Quote: ... 72 °C for 1 min) × 5] using NEBNext 2xMasterMix (NEB, M0541S) with Ad1_noMX and v2_Ad2.* indexing primers followed by qPCR amplification to determine additional cycle numbers ...
-
bioRxiv - Cell Biology 2024Quote: ... ligated to the 5’ adapter with T4 RNA ligase (NEB M0204L), and reverse transcribed with Superscript III (Thermo Fisher 18080-051) ...
-
bioRxiv - Genomics 2024Quote: ... followed by 5’-hydroxyrepair in T4 polynucleotide kinase mix (NEB, M0201S) at 37 °C for additional 45 min ...
-
bioRxiv - Genomics 2024Quote: ... and 0.5 µL BST DNA Polymerase FL (NEB, 5 U/µL) and 2 µL 10x ThermoPol Buffer (NEB ...
-
bioRxiv - Genomics 2024Quote: ... add 5 U of phi29 DNA Polymerase (10 U/μL, NEB), 0.5 μL of 10 mM dNTPs ...
-
bioRxiv - Molecular Biology 2024Quote: ... 72 °C for 1 min) × 5] using NEBNext 2xMasterMix (NEB, M0541S) with Ad1_noMX and v2_Ad2.* indexing primers followed by qPCR amplification to determine additional cycle numbers ...
-
bioRxiv - Microbiology 2024Quote: ... MBTUni-13 primer (5’-ACGCGTGATCAGTAGAAACAAGG-3’) and Phusion Polymerase (NEB M0530L). PCR products were purified with PureLink PCR Purification Kit (Invitrogen K310002 ...
-
bioRxiv - Plant Biology 2024Quote: ... 5 μg of DNA were digested with MseI restriction endonuclease (NEB) and separated using 0.8% (w/v ...
-
bioRxiv - Synthetic Biology 2024Quote: ... This mixture was combined with 5 units of Esp3I (R0734, NEB) and 100 units of T4 DNA ligase in 1x T4 ligation buffer ...
-
bioRxiv - Genomics 2024Quote: ... using klenow polymerase (5 U/μl, New England Biolabs, Catalog #M0210L). Blunt ends of the fragments were ligated with T4 DNA Ligase (1U/µl ...
-
bioRxiv - Biochemistry 2024Quote: ... Unligated linker was depleted by treatment with yeast 5’-deadenylase (NEB) and RecJ exonuclease (Lucigen/Epicenter) ...
-
bioRxiv - Cell Biology 2024Quote: ... beads were resuspended in a removal mix containing 5’ deadenylase (NEB) and RecJf exonuclease (NEB ...
-
bioRxiv - Microbiology 2020Quote: ... amplified with the primers Dhm1275-1 and −2 (Table 2) using a NEBuilder HiFi DNA Assembly Kit (New England BioLabs, Ipswich, MA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2021Quote: ... 5 μl of 100 ng/μl cDNA diluted from the previous step was combined with 2 μl block mix and 2 μl nuclease free water (NEB, catalog no. AM9937), then the cDNA-block oligo mix was incubated on a thermocycler under the following condition to allow block oligo mix to bind to 5’ and 3’ end of the cDNA molecule ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... was prepared by combining 2 µL of 100 µM guide RNA (gRNA) with 2 µL of 20 µM EnGen SpyCas9 NLS (NEB, Cat. No. M0646T), and incubated at room temperature for 2-4 hours ...
-
bioRxiv - Microbiology 2020Quote: ... incubated overnight at 4°C and treated with DNAse I (New England Biolabs) at 37°C for 60 minutes ...