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Citations for New England Biolabs :
1251 - 1300 of 7781 citations for 7 Amino 1 3 dimethyl 1H 8H pyrido 2 3 d pyrimidine 2 4 5 trione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and mRNA Cap 2’-O-methyltransferase (NEB, M0366S). Then ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µL of Lambda Protein Phosphatase (NEB, P0753S) was added to aliquots 3 and 4 (PPase only) ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µM RNCs or empty 70S ribosomes (NEB) were incubated (30 min ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM vanadyl-ribonucleoside (S1402S, New England Biolabs), and 15% formamide (60345 ...
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bioRxiv - Immunology 2022Quote: ... and Vaccinia 2’ O-methyltransferase (New England Biolabs). The mRNA was purified utilizing oligo-dT affinity chromatography and encapsulated in an LNP through a modified ethanol-drop nanoprecipitation process ...
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bioRxiv - Microbiology 2022Quote: ... mixed with 2× agarose gel loading dye (NEB), and resolved on 1% ultrapure agarose gel (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... mixed with 2× agarose gel loading dye (NEB), and resolved on 1% ultrapure agarose gel (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... 2 µL Thermostable RNase H (NEB, CN E7850), and 1 µL nuclease-free H2O ...
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bioRxiv - Biochemistry 2023Quote: ... 2 μl Taq DNA ligase (80 U, NEB), and 1 μl T4 DNA Polymerase (1 U ...
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bioRxiv - Biochemistry 2023Quote: ... 2 μl 10x Taq DNA Ligase buffer (NEB), 2 uL dNTP Solution Mix (10 mM stock ...
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bioRxiv - Cell Biology 2023Quote: ... 2 U of beta-agarose I (NEB, M0392), and 5 μL of Maxima H minus reverse transcriptase ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 units of T4 polynucleotide kinase (NEB, M0201S), 0.09 nM dNTPs and 0.045 μg/μL of BSA at 12°C for 30 min ...
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bioRxiv - Genomics 2024Quote: ... 2 µL of 10 mM ATP (NEB P0756S), 1 µL of E ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 µL of 10 mM ATP (NEB, #B0756AVIAL), 1 µL of E ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 U/mL yeast inorganic pyrophosphatase (NEB®) and 1000 U/mL murine RNase inhibitor (NEB®) ...
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bioRxiv - Microbiology 2024Quote: ... and 2 μl RppH (NEB product number M0356S), followed by incubation at 37°C for 1 hr ...
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bioRxiv - Genomics 2024Quote: ... contained 2 μL ENDOIV (NEB, 10 U/µL), 1 μL BST DNA Polymerase FL (NEB ...
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bioRxiv - Microbiology 2024Quote: ... 2 µL 10X Exonuclease I reaction buffer (NEB), 13 µL water ...
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bioRxiv - Immunology 2024Quote: ... 2 uL of Rapid PNGase F Buffer (NEB) was added and incubated at 80°C for 3 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... Then 2 μL 10x RNA ligation buffer (NEB), 0.2 μL 100 mM ATP ...
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bioRxiv - Systems Biology 2024Quote: ... 2 µL of 10X Thermo PCR buffer (NEB), 2 µL of ES-E14 wildtype cDNA template or from 1 µL plasmid pLJM1-EGFP for GFP esiRNAs ...
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Anti-HIV-1 Effect of the Fluoroquinolone Enoxacin and Modulation of Pro-viral hsa-miR-132 ProcessingbioRxiv - Molecular Biology 2024Quote: ... 10 units of T4 dsRNA Ligase 2 (NEB) were then added to the solution and incubated for 1 hour at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... 2 μl of Truncated T4 RNA Ligase (NEB), 1X ligation buffer (NEB) ...
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bioRxiv - Biophysics 2024Quote: ... and HindIII (NEB, R3104S, 2 unit/μg DNA) of Plasmid pUC19 (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µl of 20 mg/ml RNaseA (NEB) was added to the reaction and the reaction was incubated at room temperature for 5 minutes before quenching the reaction with 50 mM EDTA ...
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bioRxiv - Genetics 2024Quote: ... containing 2 U of T5 exonuclease (NEB, M0363S), 12.5 U of Phusion DNA polymerase (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... 2 kU of Taq DNA ligase (NEB, M0208S), 0.2 M Tris-HCl (pH 7.5) ...
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bioRxiv - Biophysics 2024Quote: ... using T4 RNA ligase 2 (New England Biolabs).
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bioRxiv - Biochemistry 2024Quote: ... 2) the NEBNext End Repair Module (NEB, E6050S) was used for repair of DNA ends after shearing ...
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bioRxiv - Genomics 2024Quote: ... and 2 U Quick CIP (NEB, cat# M0525S) in digestion buffer (10 mM Tris ...
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bioRxiv - Genomics 2024Quote: ... with 2% BSA and resuspended in NEB+ (NEB with 2% BSA ...
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bioRxiv - Synthetic Biology 2021Quote: ... Mixes were subsequently digested 1h at 37 °C with DpnI (NEB) and used for electroporation in E ...
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bioRxiv - Molecular Biology 2021Quote: ... and Lachnospiraceae bacterium Cpf1 (LbCpf1) (200 nM) were independently preincubated with sgRNA (600 nM) in cleavage buffer [1× NEBuffer 3 (New England Biolabs, Ipswich, MA), 10 mM DTT ...
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bioRxiv - Neuroscience 2021Quote: ... Enzyme restriction with DpnII was performed in 30 µl reaction mix (1 µl DpnII, 3 µl DpnII buffer, 10 µl purified DNA and 16 µl H2O; New England Biolabs, Ipswich, MA) and incubation at 37°C for 2h ...
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bioRxiv - Genomics 2021Quote: ... nascent RNA samples were processed through the following steps: (i) 3′ adapter ligation with T4 RNA Ligase 1 (NEB, Ipswich, MA; M0204), (ii ...
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bioRxiv - Cell Biology 2020Quote: ... primers 1 - 3) and then inserting the resulting PCR product into BamHI-digested pBMN-mCherry using Gibson assembly (New England Biolabs, Ipswich, MA). The resulting pBMN-ARHGAP36-mCherry vectors with XhoI and SacII restriction sites were subsequently used in all experiments described herein.
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bioRxiv - Molecular Biology 2020Quote: ... and 0.1% Tween) with 3% BSA and then incubating the strips in 1:2000 dilution of monoclonal anti-MPB-HRP antibody (NEB, Inc, USA) in a blocking buffer for 1 h at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... was annealed with primer P3 (100 bp long) at a 1:3 DNA to primer molar ratio and ligated using T4 DNA ligase (NEB, catalog #M0202S). Primer P3 had biotin modification at its 3’ end ...
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bioRxiv - Immunology 2023Quote: ... using primers that overlapped with pLenti-EF1-Blast at the xho1 site on the 5’ end and overlapped with the 5’-end of the RNase L coding sequence on the 3’-end (Supplementary table 1) and NEBNext® High-Fidelity 2X PCR Master Mix (New England Biolabs: M0541S). RNase L coding sequence was amplified using a 3’-end primer that overlapped with pLenti-EF1-Blast at the xba1 site ...
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bioRxiv - Cancer Biology 2023Quote: ... The purified shESR1 and SGEN DNA were digested with XhoI and EcoRI and subsequently ligated at a molar ratio of approximately 3:1 with 10X T4 DNA Ligase Buffer (New England BioLabs Inc. #B0202S) and T4 DNA Ligase (New England BioLabs Inc ...
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bioRxiv - Genomics 2022Quote: ... RNA samples were then processed through the following steps: (i) 3′ adapter ligation with T4 RNA Ligase 1 (NEB, Ipswich, MA; M0204), (ii ...
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bioRxiv - Synthetic Biology 2024Quote: ... and then ligated by Goldengate assembly with an insert-to-vector ratio as 3:1 and the T4 ligase (New England Biolabs, Cat# M0202L), following the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... 2 μl of 10X CutSmart® buffer (1X final) and 2 μl of 1U/μl USER® II enzyme (M5505S, NEB) (0.1U/μl final ...
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bioRxiv - Microbiology 2020Quote: ... stained with a mAb cocktail composed of SARS-CoV-2 spike (Creative-Bios; 2BCE5) and SARS-CoV-2 nucleoprotein (Creative-Biolabs; NP1C7C7) followed by anti-Mouse IgG-HRP (Abcam ab6823 ...
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bioRxiv - Genomics 2021Quote: ... and supernatant was transferred to a new tube and added to 2 μL 10% SDS and 2 μL 20 mg/mL proteinase K (New England Biolabs P8107). Samples were incubated overnight at 65°C to reverse crosslinks ...
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bioRxiv - Genetics 2023Quote: ... 5 µl of 100 ng/µLcDNA diluted from the previous step was combined with 2 µL of block mix and 2 µL of nuclease free water (NEB, AM9937), and then the cDNA block oligo mix was incubated on a thermocycler under the following conditions to allow block oligo mix to bind to the 5′ end and the 3′ end of the cDNA molecule ...
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bioRxiv - Microbiology 2023Quote: ... antibiotic resistance cassette and 2 flanking regions were combined in equimolar amounts and mixed with 2 x HiFi reagent (NEB, UK) and incubated at 50°C for 1 hour ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 1×Buffer 4 (NEB). The reaction was stopped (6 mM EGTA ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4-5 cycles of PCR with OneTaq polymerase (New England Biolabs) was performed using the forward (pBPS_fwr ...
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bioRxiv - Cell Biology 2020Quote: ... 3 µg of purified DNA was digested with 10 units I-SceI (NEB, R0694), 10 units I-CeuI (NEB ...