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Citations for New England Biolabs :
1201 - 1250 of 1359 citations for F actin uncapping protein LRRC16A CARMIL1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The pRL814 (containing green fluorescent protein (GFP) under control of PT7A1-O34) backbone was digested with NdeI and HindIII-HF (NEB). The digested backbone and amplified genes were ligated using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Biochemistry 2024Quote: PamB2-ribosome complexes were generated by in vitro transcription-translation reactions in PURExpress in vitro protein synthesis system (New England Biolabs) with the same reaction mix as described earlier in the toeprinting assays ...
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bioRxiv - Plant Biology 2024Quote: ... MBP-RVE4 and MBP-RVE8 proteins were expressed in Escherichia coli BL21 strain according to the manufacturer’s instructions using the pMAL Protein Fusion and Purification System (New England Biolabs; #E8200) and purified using MBPtrap HP column (Cytiva ...
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bioRxiv - Synthetic Biology 2024Quote: ... where separately introduced to the wild-type MS2 coat protein present in these mRNA constructs using the NEB-Q5 mutagenesis kit (New England Biolabs). The primer sets and PCR settings used are listed in supplementary information ...
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bioRxiv - Microbiology 2024Quote: ... SINV-REP construct derived from pToto64 clone encoding SINV non-structural proteins and a reporter luciferase tag was linearized using SacI (New England Biolabs), followed by in vitro transcription using SP6 RNA polymerase (New England Biolabs) ...
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bioRxiv - Cell Biology 2024Quote: ... Reactions were quenched by addition of EDTA to 50 mM and proteins were removed by treatment with proteinase K (20 units/ml) (NEB) and SDS (0.25% ...
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bioRxiv - Neuroscience 2024Quote: ... The beads were washed three times with PNK wash buffer and the RNA-protein complexes labeled with 32P with the following reaction: 4 μl 10X PNK buffer (NEB), 2 μl T4 PNK enzyme ...
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bioRxiv - Genomics 2024Quote: ... and then RCA amplified overnight at 30oC using Phi29 DNA polymerase from the nick site with T4 gene 32 single-stranded binding protein (SSB) (New England Biolabs). RCA amplified DNA was ethanol precipitated and resuspended in CutSmart buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Sequences coding 3×HA or 3×HA-GFP tags were added to the C-terminal of the protein using gene-specific PCR and HiFi assembly (NEB). The 3×HA and 3×HA-GFP sequences were cloned from pMXs-3XHA-EGFP-OMP25 plasmid (Addgene 83356).
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bioRxiv - Synthetic Biology 2024Quote: ... 10 µL of the reaction mixture was used to purify the proteins contained in the PURE system using a His-tag spin column (New England Biolabs), according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... The XPF and ERCC1 constructs for expression of XPF and ERCC1 proteins were fused by Cre recombinase (New England Biolabs). The constructs for SLX4 and XPF-ERCC1 were then transformed to E ...
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bioRxiv - Evolutionary Biology 2024Quote: The mRNAs for cell-free protein expression were transcribed using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Supernatant fluids containing GST-fused proteins or MBP-fused proteins were incubated with Glutathione Sepharose 4B beads (#17-0756-01; Cytiva) or amylose resin (#E8021; New England Biolabs), respectively ...
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bioRxiv - Cell Biology 2024Quote: ... were cloned into the pGEX-6P1 vector using BamHI/XhoI restriction sites and expressed as a 3C protease-cleavable N-terminal GST-fusion protein in BL21 (DE3) cells (NEB). A 20mL LB overnight culture was inoculated into 1L of LB medium supplemented with 100μg/mL ampicillin to an OD600 of 0.05 and incubated at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: Specific antibodies against Slug (C19G7, Cell Signaling Technology, NEB, Frankfurt, Germany, #9585, 1:400), pan-cytokeratine (polyclonal ...
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bioRxiv - Immunology 2022Quote: ... As an isotype control an unspecific polyclonal rabbit antibody was used (NEB, Frankfurt, Germany). To block ORF8 during differentiation it was preincubated with the polyclonal rabbit anti-ORF8 antibody or isotype over night and added to the monocytes (t=0 ...
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bioRxiv - Microbiology 2021Quote: ... plates were immunostained using a monoclonal antibody against SARS-CoV2 nucleoprotein (Creative-Biolabs; NP1C7C7) at a dilution of 1:1000 followed by 1:5000 anti-mouse IgG monoclonal antibody and was developed using KPL TrueBlue peroxidase substrate for 10 minutes (Seracare ...
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Spatial visualization of A-to-I Editing in cells using Endonuclease V Immunostaining Assay (EndoVIA)bioRxiv - Molecular Biology 2024Quote: ... Cells were then incubated for 1 hour with anti-MBP antibody (New England BioLabs) solution diluted in calcium containing blocking buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... Fgfrb-eGFP signal was detected using an anti-GFP antibody (TP401, Torrey Pine Biolabs) in combination with nuclear (Hoechst ...
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bioRxiv - Biochemistry 2024Quote: ... or fluorescently labelled secondary antibodies diluted 1:15000 (Cell Signaling Technology, NEB, Hitchin, UK), for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: In vitro trans-translation assays were performed using the PURExpress In Vitro Protein Synthesis and Δ Ribosome kits (New England Biolabs). For trans-translation assays ...
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bioRxiv - Genomics 2021Quote: ... Samples were then combined and AMII adapters containing the motor proteins needed for sequencing were ligated using NEBNext® Quick Ligation Module (NEB). AMPure XP beads (Beckman Coulter ...
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bioRxiv - Biochemistry 2020Quote: ... Beads were resuspended in 1 volume of buffer A / 300 mM NaCl / 2 mM MnCl2 / 1 mM DTT and incubated with λ protein phosphatase (NEB) at 50 U / mL for 1 hour at 23°C with agitation ...
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bioRxiv - Biochemistry 2021Quote: ... The RNAs were recovered from the gel slices by digesting the protein with proteinase K (8 U; P8107S, NEB, Ipswich, MA) leaving a polypeptide remaining at the crosslinked nucleotide ...
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bioRxiv - Plant Biology 2020Quote: 20-33 kD subregions of NRPD1 and RDR2 polypeptides were expressed in vitro using a PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs). Briefly ...
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bioRxiv - Immunology 2022Quote: Single mutations of the spike protein were generated via two PCR fragments of the spike ORF using high-fidelity Phusion polymerase (New England Biolabs, USA). The first fragment was generated via a generic forward primer (pCAGGS-5 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a total of 575 embryos were injected with a mixture containing 300 ng/μL of sgRNA (one guide) and 600 ng/μL of Cas9 protein (NEB, M0641) while for Dll ...
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bioRxiv - Microbiology 2022Quote: ... The SARS-CoV-2 Spike mutant D614G was generated by site-directed mutagenesis using as an input DNA the expression vector encoding SARS-CoV-2 Spike_614D protein (kindly provided by J. Garcia-Arriaza, CNB-CSIC) by Q5 Site Directed Mutagenesis Kit (New England Biolabs, Barcelona, Spain) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: The fusion protein was purified from the clarified supernatants by adding 20 ml of chitin resin (New England Biolabs, cat#S6651S) and incubating with gentle rotation overnight at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The non-15N 4R tau used for small-scale chemical in vitro crosslinking was phosphorylated using cAMP-dependent Protein Kinase (PKA, catalytic subunit, New England Biolabs #P6000S) according to manufacturer guidelines (25uL reaction at 30°C for 2 hours with 200 μM ATP ...
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bioRxiv - Synthetic Biology 2021Quote: ... and a maltose binding protein (MBP) (65) and Factor Xa sequence derived from pMAL-c5X vector (New England Biolabs, Ipswich, MA) with a V313A mutation to be consistent with the native E ...
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bioRxiv - Bioengineering 2020Quote: ... 200-500 ng of the purified PCR product was used as DNA template in 25 μl of coupled in vitro transcription and translation reaction using PURExpress In Vitro Protein Synthesis Kit (New England Biolabs, E6800L). The reaction was incubated at 37°C for 30 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... After annealing the complex an equimolar amount was mixed with 1000 ng Cas9 recombinant protein (NEB; final concentration 20 ng/μL) and incubated at RT for 15 min ...
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bioRxiv - Synthetic Biology 2021Quote: 8.3 μM bdSUMO-HSPB611–20 fusion protein containing pSer or nhpSer at site S16 of HSPB6 were reacted with 3 units of λ phosphatase (NEB) according to manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2021Quote: ... the TEV protease was removed by incubation of the purified protein with 250 µL amylose resin (New England Biolabs, Hitchin, UK). Protein concentrations were determined by the bicinchoninic acid assay (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... we constructed the genetic sequence of the major shell protein of 50nmGVs using Q5 DNA polymerase and Gibson assembly kits (New England Biolabs Inc.)65 ...
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bioRxiv - Microbiology 2023Quote: ... Each protein was then isolated and affinity-purified using an amylose resin according to the manufactureŕs protocol (New England Biolabs, Ipswich, US) and the concentration was determined using the Bradford technique ...
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CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signallingbioRxiv - Immunology 2023Quote: ... WT SUMO3-CXCL17 and variants were expressed as soluble proteins in SHuffle® T7 Competent E.coli (New England Biolabs, Hitchin, UK). In both protocols ...
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bioRxiv - Biochemistry 2024Quote: ... the crude extract containing 100 μg (in 100 μL volume) of proteins was treated with 400 units of λ-PPase (#P0753; New England Biolabs) for 60 min at 30 °C ...
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bioRxiv - Biophysics 2023Quote: Tau was phosphorylated in vitro according to previous protocols with some modifications.12,88 50 µL of 100 µM tau was incubated with 2 µg cAMP-dependent Protein Kinase (PKA, New England BioLabs P6000S) and/or 0.4 µg GSK3β (Sigma-Aldrich G4296 ...
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bioRxiv - Cell Biology 2024Quote: All purified SNAP-tagged proteins were labeled with 10-fold molar excess of SNAP-Surface dyes (New England Biolabs, Ipswich, MA) in labeling buffer (1× PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Unligated 3’ linker was removed by incubating the samples with the 5’-deadenylase KmHnt3 (see Recombinant protein expression and purification) and RecJ exonuclease (New England Biolabs, M0264S) for 45 min at 37 °C ...
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bioRxiv - Biophysics 2023Quote: ... Trypsin digestion was initiated by adding 1:10 (trypsin to protein, m/m) mass spectrometry grade trypsin (New England Biolabs #P8101S) in 50 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2022Quote: ... for each co-expressed DF-APOBEC1 protein using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760L) with NEBNext Poly(A ...
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bioRxiv - Microbiology 2022Quote: ... Purified protein (4 μg) was deglycosylated with 500 U of Endoglycosidase H (Endo H) (New England Biolabs, Ipswich, MA, United States) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... Strep–PIF4 recombined proteins were expressed in the Escherichia coli BL21 (DE3) strain and then purified using amylose resin (NEB, E8021S). DNA probes of NHX1 and SAL1 were synthesized and labeled using the Biotin 3′ End DNA Labeling Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was then purified by affinity-purification using an amylose beads according to the manufacturer’s protocol (New England Biolabs, Ipswich, USA) and the concentration determined by Bradford assay ...
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bioRxiv - Cell Biology 2023Quote: Beads with bound Flag-RAD50 or GFP-BRCA1 were washed with 5M NaCl to eliminate contamination with protein kinases and incubated with 100 or 500 U of CK2 holoenzyme complex (α2/β; NEB) in 30 μl of kinase buffer (20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2024Quote: The cell lysates or purified PINK1 bound to FLAG beads were incubated at 30°C for 1h with 200 units of ʎ protein phosphatase (LPP) in a total reaction of 100µL following the instructions of the manufacturer (New England Biolabs #P0753).
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bioRxiv - Cell Biology 2024Quote: We fused SNAP-3xHA coding sequences downstream H3.1 and H3.3 CDS 46 and inserted this fusion protein into the pBS31 vector 70 using NEBuilder® HiFi DNA Assembly Master Mix (NEB). These expression vectors (pB31-H3.1- and pB31-H3.3-SNAP-3xHA ...