Labshake search
Citations for New England Biolabs :
1201 - 1250 of 10000+ citations for Epiandrosterone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... Small RNAs were 3’ dephosphorylated and 5’ phosphorylated with T4 polynucleotide kinase (T4PNK, NEB, #M0201S), and purified with RNA Clean and Concentrator-5 (Zymo Research ...
-
bioRxiv - Molecular Biology 2023Quote: ... 6 μL 20mg/ml BSA and 5 μL 400U/μl T4 DNA Ligase (NEB, M0202) overnight at 16°C (tumbling ...
-
bioRxiv - Biophysics 2023Quote: ... Calmodulin and myosin-5 expression plasmids were prepared from E.coli 5α cells (New England Biolabs), transformed with the each of plasmids ...
-
bioRxiv - Genomics 2023Quote: ... followed by A tailing with NEB Klenow Fragment (3’−5’ exo-) (New England Biolabs, M0212), adapter ligation with NEB DNA Quick Ligase (New England Biolabs ...
-
bioRxiv - Genomics 2023Quote: ... the single-stranded probes were further digested with 5 units of USER enzyme (NEB M5505L) in 1x DpnII buffer at 37 °C for 3 hours.
-
bioRxiv - Immunology 2023Quote: ... The diluted supernatant was loaded onto a 5 mL amylose resin column (New England Biolabs). The column was washed with 150 mL of buffer I ...
-
bioRxiv - Genetics 2023Quote: ... DNA was circularized at a concentration of 5 ng/μL with T4 DNA ligase (NEB), and treated with a cocktail of exonucleases ...
-
Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... the isolated RNA was treated with 1 μl (5 units) RppH enzyme (New England Biolabs) in NEB Buffer 2 (50 mM NaCl ...
-
bioRxiv - Cell Biology 2023Quote: ... Plasmids were amplified in chemically competent 5-alpha F’Iq Escherichia coli cells (New England Biolabs) after 42°C heat shock transformation and extracted using the GenElute HP Plasmid Miniprep Kit (Sigma-Aldrich ...
-
bioRxiv - Plant Biology 2023Quote: ... Ligation was performed by increasing the volume 5 times with T4 DNA ligase buffer (NEB) to a final concentration of 1X ...
-
bioRxiv - Microbiology 2023Quote: ... The 5’ ends were radiolabelled with 20 μCi γ32P-ATP using T4 polynucleotide kinase (NEB) and separated from free nucleotides using a MicroSpin G-50 column (Cytiva) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Exonuclease treatment was performed by addition of 5 µL of NEBbuffer1 (NEB, cat. no. B7001S) and 1 µL of each Exonuclease I (NEB ...
-
bioRxiv - Cell Biology 2023Quote: ... using 5 μl of the reaction with 1 μl of loading dye (New England Biolabs). Agarose gel was electrophoresed at 70V until resolution of bands required was reached ...
-
bioRxiv - Molecular Biology 2023Quote: RNA was denatured for 5 min at 95°C in 1x RNA Loading Dye (NEB) and separated on denaturing polyacrylamide gels (12% ...
-
bioRxiv - Molecular Biology 2023Quote: ... 55 Excess adaptor was removed by incubation with 5′ deadenylase and the exonuclease RecJf (NEB). TIS11B protein-RNA complexes were eluted from the beads by heating at 70°C for one minute ...
-
bioRxiv - Molecular Biology 2023Quote: ... Probes were synthesized as ssDNA oligos and 5’ 32P-radiolabeled with T4 polynucleotide-kinase (NEB) and ψ-32P-ATP (6000 Ci/mmol ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids were amplified using NEB 5-alpha F′ Iq competent Escherichia coli (NEB, Cat# C2992H) and extracted using the PureYield Plasmid Miniprep System (Promega ...
-
bioRxiv - Biochemistry 2024Quote: ... In short 5’-phosphorylated and 3’-OH RNA was circularized with RNA ligase 1 (NEB) followed by denaturing PAGE purification as described above ...
-
bioRxiv - Microbiology 2024Quote: ... and was [32P]-labelled at the 5’-end using T4 polynucleotide kinase (New England BioLabs) and purified as previously described (55) ...
-
bioRxiv - Cell Biology 2024Quote: ... These cells that were pre-stained with 5 µM SNAP-Surface 647 (New England Biolabs) for one hour at room temperature if necessary were stained with 5µg/mL of Alexa488 labeled lectins (PNA ...
-
bioRxiv - Genetics 2024Quote: All plasmids were transformed into NEB 5-alpha Competent Escherichia coli cells (New England Biolabs) and purified using Qiaprep Spin Miniprep Kits or Plasmid Plus Maxi Kits for plasmid libraries (QIAGEN) ...
-
bioRxiv - Plant Biology 2024Quote: ... 5 µg of RNA was used to synthesize cDNA by oligo dT (New England Biolabs) and reverse transcriptase (Thermo Fisher) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 5 μg of plasmid DNA of ACT1 was linearized using SpeI restriction enzyme (NEB, USA), purified by Phenol:Chloroform extraction and re-suspended in 10 μl of RNase free sterile water (Raut et al. ...
-
bioRxiv - Biochemistry 2024Quote: In-vitro synthesized pri-miRNAs (100 pmol) were 5’-end labelled by T4-PNK (NEB) using [γ-32P]-ATP (Perkin Elmer ...
-
bioRxiv - Biophysics 2021Quote: Polymerised gels were removed from the coverslip chamber and placed into 6-well plates to undergo digestion in 0.2 mg/ml proteinase K (New England Biolabs) dissolved in digestion buffer (50 mM Tris pH 8.0 (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2019Quote: ... pellet samples were collected from the qRT-PCR plate and mixed with Purple Gel Loading Dye (BioLabs, Cat#B7025S) to be load on 1% agarose gel ...
-
bioRxiv - Neuroscience 2019Quote: Single nucleus RRBS were performed by first sorting PROX+/FOS+ and /FOS- neuronal nuclei in 96-well plates in 3 µL of 0.1× CutSmart buffer (New England Biolabs) per well as described in the Flow Cytometry section of the Materials and Methods ...
-
bioRxiv - Genomics 2023Quote: ... cells in the latter plate were labeled with HaloTag Oregon Green and SNAP-Cell 647-SiR (New England Biolabs) simultaneously according to the manufacturers’ protocols ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Each plate’s DNA library was then amplified in two 50 µl Q5 High-Fidelity DNA Polymerase reactions (New England BioLabs) for 11 thermocycles ...
-
bioRxiv - Biochemistry 2019Quote: ... we used a commercial assay kit (Ph.D.-7 Phage Display Peptide Library Kit, New England Biolabs) and followed the recommended protocol for “solution phase panning with affinity bead capture” with the following modifications ...
-
bioRxiv - Biophysics 2023Quote: ... Plasmid DNA molecules were purified using a commercially available purification kit (MonarchQR Plasmid Miniprep Kit, NEB). The purified plasmids were run on a 0.8% agarose gel supplemented with 2.5 µg/mL of chloroquine in 1xTBE buffer containing 2.5 µg/mL of chloroquine at 25 V for 15 hours ...
-
bioRxiv - Plant Biology 2020Quote: ... using the Gibson assembly kit (NEB). The pET28b vector was linearized by digesting with BamHI and XhoI to create overhangs compatible for Gibson assembly ...
-
bioRxiv - Biophysics 2020Quote: Using PURExpress kits (New England Biolabs), 37.5 μL IVT reactions were assembled on ice with 1.5 μl RNase inhibitor ...
-
bioRxiv - Microbiology 2020Quote: ... using the NEBuilder HiFi kit (NEB).
-
bioRxiv - Microbiology 2020Quote: ... using the NEBuilder HiFi kit (NEB). The mdtU-lacZ translational fusions were constructed by PCR-amplifying the mdtU gene from either a WT (E ...
-
bioRxiv - Molecular Biology 2022Quote: ... NEBNext rRNA Depletion kit( NEB #E7405) was used to deplete ribosomal RNA ...
-
bioRxiv - Molecular Biology 2021Quote: ... USA (NEB PCR Cloning Kit E1203S) were used for the sub-cloning of DNA fragments and to create plasmid templates for run-off in vitro transcription ...
-
bioRxiv - Cell Biology 2019Quote: ... using Gibson assembly cloning kit (NEB). COX8A-SNAP vector was commercially obtained from NEB ...
-
bioRxiv - Genomics 2021Quote: ... NEBNext UltraII Q5 kit (NEB M0544) was used for PCR enrichment ...
-
bioRxiv - Microbiology 2021Quote: ... using Monarch Plasmid Miniprep kit (NEB). Sequencing was through the company Genewiz.
-
bioRxiv - Molecular Biology 2021Quote: ... Q5 Site-Directed Mutagenesis Kit (NEB) was used for generating dCas9 plasmids encoding the mutant p300core* (Y1467F ...
-
bioRxiv - Immunology 2022Quote: ... The second strand synthesis kit (NEB) at 16°C for 2 hours was used for cDNA synthesis followed by a 1.4X AMPure XP bead cleanup ...
-
bioRxiv - Cell Biology 2022Quote: ... Q5 site directed mutagenesis kit (NEB) was used and the manufacturer’s instructions were followed.
-
bioRxiv - Biophysics 2020Quote: ... and Q5 Hotstart Mutatagenesis kit (NEB) were used.
-
bioRxiv - Genomics 2022Quote: Monarch Genomic DNA Purification kit (NEB)
-
bioRxiv - Developmental Biology 2022Quote: ... “Q5 Site-Directed Mutagenesis Kit” (NEB) was used ...
-
bioRxiv - Neuroscience 2020Quote: ... using the Gibson assembly kit (NEB) to create pBPGUw-QF2 ...
-
bioRxiv - Developmental Biology 2021Quote: ... HiScribe T7 ARCA mRNA kit (NEB) was used for mRNA synthesis with 10 μl of 2x ARCA/NTP mix ...
-
bioRxiv - Microbiology 2022Quote: ... or Monarch RNA isolation kit (NEB) was used for RNA extraction ...
-
bioRxiv - Immunology 2021Quote: ... end blunting (Quick blunting kit; NEB), and ligation with (T4 ligase ...