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Citations for New England Biolabs :
1151 - 1195 of 1195 citations for Recombinant Human PINX1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: All purified SNAP-tagged proteins were labeled with 10-fold molar excess of SNAP-Surface dyes (New England Biolabs, Ipswich, MA) in labeling buffer (1× PBS ...
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bioRxiv - Biochemistry 2024Quote: ... the crude extract containing 100 μg (in 100 μL volume) of proteins was treated with 400 units of λ-PPase (#P0753; New England Biolabs) for 60 min at 30 °C ...
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bioRxiv - Biophysics 2023Quote: Tau was phosphorylated in vitro according to previous protocols with some modifications.12,88 50 µL of 100 µM tau was incubated with 2 µg cAMP-dependent Protein Kinase (PKA, New England BioLabs P6000S) and/or 0.4 µg GSK3β (Sigma-Aldrich G4296 ...
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CXCL17 binds efficaciously to glycosaminoglycans with the potential to modulate chemokine signallingbioRxiv - Immunology 2023Quote: ... WT SUMO3-CXCL17 and variants were expressed as soluble proteins in SHuffle® T7 Competent E.coli (New England Biolabs, Hitchin, UK). In both protocols ...
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bioRxiv - Microbiology 2023Quote: ... Each protein was then isolated and affinity-purified using an amylose resin according to the manufactureŕs protocol (New England Biolabs, Ipswich, US) and the concentration was determined using the Bradford technique ...
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bioRxiv - Synthetic Biology 2023Quote: ... we constructed the genetic sequence of the major shell protein of 50nmGVs using Q5 DNA polymerase and Gibson assembly kits (New England Biolabs Inc.)65 ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was then purified by affinity-purification using an amylose beads according to the manufacturer’s protocol (New England Biolabs, Ipswich, USA) and the concentration determined by Bradford assay ...
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bioRxiv - Cell Biology 2023Quote: Beads with bound Flag-RAD50 or GFP-BRCA1 were washed with 5M NaCl to eliminate contamination with protein kinases and incubated with 100 or 500 U of CK2 holoenzyme complex (α2/β; NEB) in 30 μl of kinase buffer (20 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Biophysics 2023Quote: ... Trypsin digestion was initiated by adding 1:10 (trypsin to protein, m/m) mass spectrometry grade trypsin (New England Biolabs #P8101S) in 50 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2024Quote: The cell lysates or purified PINK1 bound to FLAG beads were incubated at 30°C for 1h with 200 units of ʎ protein phosphatase (LPP) in a total reaction of 100µL following the instructions of the manufacturer (New England Biolabs #P0753).
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bioRxiv - Cell Biology 2024Quote: We fused SNAP-3xHA coding sequences downstream H3.1 and H3.3 CDS 46 and inserted this fusion protein into the pBS31 vector 70 using NEBuilder® HiFi DNA Assembly Master Mix (NEB). These expression vectors (pB31-H3.1- and pB31-H3.3-SNAP-3xHA ...
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bioRxiv - Molecular Biology 2020Quote: Cleavage of the MBP tag from NlGr7 was completed according to manual instructions of the pMAL protein fusion and purification system (NEB, Inc, USA). The tag (MBP ...
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bioRxiv - Molecular Biology 2020Quote: ... the desired sequences pertaining to the truncated forms of the N protein were subcloned from the synthetic gene using polymerase chain reaction (Phusion polymerase, New England Biolabs, Hertfordshire, UK). All genes were cloned into the modified pet28 vector and gene sequences were confirmed by Sanger Sequencing.
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bioRxiv - Biochemistry 2022Quote: ... purified Trx-FER-KD and FER-KDK565R proteins were treated with 1 μl of λ-phosphatase (λ-PP) (400,000 units/ml, New England Biolabs. P0753S) and 1 mM MnCl2 for 1-2 h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... protease mutant S219V [24] was inserted at the 3’ end of gene encoding maltose binding protein (MBP) in pMAL-c5E vector (New England Biolabs, MA, USA) to construct pMAL-TEV vector ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 μl of the gRNA mixture was mixed with Cas9-NLS protein (final concentration of 5 μM. New England Biolabs, Ipswich, USA), 2M KCl (final concentration of 300mM) ...
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bioRxiv - Microbiology 2021Quote: ... 39 μl of each lysate was combined with 5 μl of 10X NEB buffer for Protein MetalloPhosphatases (New England Biolabs, Ipswich, MA), 5 μl of 10 mM MnCl2 and 1-2 μl (400-800 units ...
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bioRxiv - Genetics 2020Quote: ... Zebrafish embryos were injected at the 1-cell stage with a 1 nL solution of bbs2 gRNA (200 ng/μL) and Cas9 protein (10 μM; New England BioLabs, Beverly, MA). Mutagenesis was confirmed by High Resolution Melt Analysis (HRMA ...
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bioRxiv - Molecular Biology 2022Quote: ... deglycosylation treatment (Dglyco) consisted of sample incubation for 3 hours at 37°C with 20 nL of Protein Deglycosylation Mix II (NEB, cat. P6044S) per μL of sample ...
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bioRxiv - Microbiology 2024Quote: ... 39 μl of each benzonase-treated lysate was combined with 5 μl of 10X NEB buffer for Protein MetalloPhosphatases (New England Biolabs, Ipswich, MA), 5 μl of 10 mM MnCl2 and 1 μl (400 units ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 - 400 ng of PCR fragments were used as template DNA to synthesize analytic amounts of DNA deaminases using PURExpress In Vitro Protein Synthesis kit (NEB, Ipswich, MA) following manufacturer’s recommendations.
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bioRxiv - Evolutionary Biology 2024Quote: ... or four (white shavenbaby experimental) sgRNA targeting the first or second exon (100 ng/μl) and CAS9 protein (EnGen Spy Cas9 NLS from NEB, Catalog # M0646M). The distribution of the major trichome height for the white control was normal (Shapiro-Wilk normality test W = 0.9637 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins (except from cells expressing eGFP and cytoplasmically tagged EGFR) were further treated with 250 U of PNGase F (NEB, Cat# P0704) for 1 hour at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Electrophoresis was conducted in MES buffer at 100-120 V for 40 min with protein standards (NEB, P7119S, or Bio-Rad, #1610373). Proteins were transferred to nitrocellulose membrane and membranes blocked in 5% milk powder/PBS overnight at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... A pre-cleaning step was applied by incubating each sample with 10 μl of Protein A magnetic beads (New England Biolabs GmbH, Frankfurt, Germany) for 1 h at 4 °C in tube roller (15 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... The DNA encoding the EGFP-fusion proteins and the P2A peptide was inserted by Gibson assembly using NEBuilder HiFi DNA assembly master mix (New England BioLabs, Ipswich, MA, USA). The HeLa Flp-In T-REx K44A Dynamin2-EGFP-P2A-Caveolin1-mCherry construct was obtained by in vitro mutagenesis of the pcDNA/FRT/TO/Dyn2-EGFP-P2A-Caveolin1-mCherry construct exchanging lysine at position 44 to an alanin using these primers ...
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bioRxiv - Developmental Biology 2020Quote: ... A total of 863 Bicyclus embryos were injected with 300 ng/µl of guide and 300 ng/µl of Cas9 protein (NEB; Cat. no.: M0641) mixed together in equal parts (total volume = 10 µl ...
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bioRxiv - Developmental Biology 2019Quote: ... The bound protein (~42kDa, 360 amino acids) was released from the MBP tag by cleavage with Factor Xa (New England Biolabs, Massachusetts, United States) for 24 hours at 4°C in 20mMTris pH 7.2 ...
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bioRxiv - Biochemistry 2021Quote: ... and one plasmid was randomly selected from those plasmids and used for expression with a PURE system (PURExpress In Vitro Protein Synthesis Kit, New England BioLabs, Ipswich, MA, USA).
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bioRxiv - Cancer Biology 2020Quote: ... The synthetic genes encoding WT human TRIM28 with an N-terminal maltose binding protein (MBP) affinity tag (UniProt: Q13263) were subcloned to the pMAL-p2X vector (New England Biolabs, Beverly, MA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Samples were diluted to 1.0 mg/mL protein using homogenization buffer and incubated with 20 U/μL lambda phosphatase in MnCl2 and enzyme buffer as supplied with the lambda protein phosphatase kit (New England Biolabs, Evry-Courcouronnes, France) for 3 h at 30 °C ...
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bioRxiv - Bioengineering 2023Quote: ErCas12a (1 μg) or LbCas12aU (1 μg) protein and 1 μg crRNA were assembled in 1X Nuclease Reaction Buffer (New England BioLabs, Ipswich, MA, USA) at room temperature for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... corresponding to a portion of PfRNF1 (RP2; Fig. 1A) was expressed as a maltose-binding fusion protein using the pMAL™c5X-vector (New England Biolabs, Ipswich, USA). To this end ...
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bioRxiv - Microbiology 2021Quote: The rem allele was amplified from a cloning construct (primer sequences in Table S4) and ligated into pTYB12 (NEB IMPACT system of protein purification), resulting in an N-terminal intein fusion to Rem ...
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bioRxiv - Molecular Biology 2021Quote: ... original numbering with signal sequence = 20–559) were subcloned into the manufacturer-supplied plasmid from the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, Ipswich, MA, USA) using the Gibson assembly method with synthetic E ...
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bioRxiv - Microbiology 2021Quote: ... MNase activity was quenched by addition of EGTA and samples were subsequently pre-cleared by addition of 40 µl Protein A Magnetic Beads (New England Biolabs, Ipswich, MA, United States) and rotating at 4°C for 60 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Removal of both N-linked and O-linked glycans was performed by incubation with five different glycosidases: PNGaseF (New England Biolabs, 125 U/μg protein), O-glycosidase (New England Biolabs ...
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bioRxiv - Bioengineering 2022Quote: ... Processing the samples for proteomics was then continued using a 10 kDa filter aided sample preparation protocol previously published with minor modifications.[30] The proteins were digested using Lys-C enzyme (P8109S, New England Biolabs, MA, USA; 1:50) and then with trypsin (V5111 ...
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bioRxiv - Biophysics 2019Quote: ... The resulting product (typically 50 – 100 μL of 5 – 20 μM of protein) was mixed with an equimolar amount of SNAP-Surface 549 (New England Biolabs; 1 mM in DMSO) and incubated at room temperature for 30 min ...
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bioRxiv - Cell Biology 2020Quote: ... aliquots of the same protein extract were separated and treated with 200 U of Lambda Phage Phosphatase (#P0753S, New England Biolabs, Ipswich, Massachusetts, United States), as specified by the manufacturer ...
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bioRxiv - Plant Biology 2024Quote: ... when OD600 reached 0.5 for 4 h at 37 °C and then they were purified using amylose resin column (New England Biolabs, for MBP related proteins purification) or Ni-NTA agarose column (Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: Injections into fertilized eggs for knockout experiments were done using 50ng/µl of each sgRNA independently and 80nM of NEB EnGen Spy Cas9-NLS protein (New England Biolabs France Genopole, Evry, France), pre-incubated at room-temperature for 15 minutes (Hill et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein amounts of OP18 and beta-actin were quantified using a primary stathmin polyclonal antibody (1:1000; Cell Signaling Technology, NEB GmbH, Frankfurt/Main, Germany) and a polyclonal beta-actin antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... 250 μL of 2 mg mL-1 Y289L GalT,46 50 μL of 500 kU mL-1 PNGase F (New England Biolabs, 5 U μg-1 of protein), and 62.5 μL of Lambda Protein Phosphatase (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... purified KRAS protein was mixed with GMP-PNP (molar ratio of 10:1 GMP-PNP:KRAS) and calf intestinal alkaline phosphatase (NEB cat# M0290, 3 units per mg of KRAS). The reaction mixture was incubated for 3 hours at room temperature and then purified by size-exclusion chromatography on a 10/300 Superdex 75 GL column (GE Healthcare ...