Labshake search
Citations for New England Biolabs :
1151 - 1200 of 10000+ citations for Human Procollagen Type III N Terminal Propeptide PIIINP ELISA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... using Phusion© High-Fidelity PCR Kit (NEB) with annealing temperature at 62 ◦C ...
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bioRxiv - Microbiology 2024Quote: ... The Q5 NEB Mutagenesis Kit (New England Biolabs) was used to add an NES (LQKKLEELEL ...
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bioRxiv - Cell Biology 2024Quote: ... The NEB HiFi assembly kit (New England Biolabs) was used to clone the gene into a pET29b expression vector ...
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bioRxiv - Microbiology 2024Quote: ... the Q5 Site-Directed Mutagenesis Kit from NEB was used according to the protocol and mutagenesis primers were designed using NEBaseChanger.
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bioRxiv - Molecular Biology 2024Quote: ... with HiScribe T7 High Yield RNA Kit (NEB). Then 120 picomoles of RNA of the aptamer and RAI14 mutants ...
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bioRxiv - Microbiology 2024Quote: ... Luna Universal One-Step RT-qPCR Kit (NEB) was used to prepare cDNA and as reagent for RT-qPCR according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The NEBNext rRNA Depletion Kit (New England BioLabs) was employed to deplete ribosomal RNA ...
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bioRxiv - Microbiology 2024Quote: ... A Monarch PCR & DNA Cleanup Kit (NEB, T1030S) was used to purify the amplified product ...
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bioRxiv - Genomics 2024Quote: ... NEBNext Microbiome DNA Enrichment Kit (New England Biolabs) was applied to the extracted DNA to remove host eukaryotic insect DNA and increase the proportion of bacterial DNA ...
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bioRxiv - Immunology 2024Quote: ... DNA was cleaned using the Monarch kit (NEB) and quantified using a bioanalyzer (Agilent) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by Monarch Total RNA Miniprep Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The NEB HiFi assembly kit (New England Biolabs) was used to clone the gene fragment into a pET29b expression vector ...
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bioRxiv - Neuroscience 2024Quote: ... and purified with Monarch PCR cleanup kit (NEB). Purified amplicons were sequenced by Plasmidsaurus ...
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bioRxiv - Neuroscience 2024Quote: ... NEBuilder® HiFi DNA Assembly Kit (NEB, # E2621L) was used to construct the mRab12Q100L variant ...
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bioRxiv - Neuroscience 2024Quote: ... NEBNext Ultra II DNA Library Preparation Kit (NEB) was used to generate the libraries according to manufacturer9s instructions ...
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bioRxiv - Genomics 2024Quote: ... purified with a PCR purification kit (NEB, T1030S), and then subjected to Illumina sequencing with 60bp reads at 3M reads per sample (Donnelly Centre sequencing core facility).
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bioRxiv - Microbiology 2024Quote: ... aureus gDNA (Monarch genomic DNA purification kit, NEB). Gibson assembly (NEB ...
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bioRxiv - Genomics 2024Quote: ... A Monarch plasmid miniprep kit (NEB, Cat# T1110) was used to isolate the plasmids following the manufacturer protocol in which the afforded plasmids were further treated with the Nucleotide digestion mix (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... restriction digestion and Quick Ligation Kit (NEB, M2200S). Sequences coding 3×HA or 3×HA-GFP tags were added to the C-terminal of the protein using gene-specific PCR and HiFi assembly (NEB) ...
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bioRxiv - Biophysics 2024Quote: ... A Site- Directed Mutagenesis Kit (New England Biolabs) was used to generate the cDNAs encoding the OR point mutants.
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bioRxiv - Bioengineering 2024Quote: ... Q5 site-directed mutagenesis kit was from NEB. 96-well UV plates used in the assays were bought from Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... using Q5 Mutagenesis Kit (#E0554S, New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... using T4 DNA ligase (Quick Ligation Kit, NEB) at room temperature for 5–10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... and quantified using the Illumina Quant Kit (NEB). Sequencing of these libraries was performed on the Illumina NextSeq platform ...
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bioRxiv - Genetics 2024Quote: ... EpiMark® BC kit (New England Biolabs, USA), DNA BC kit I (Biovision ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The library of each sample was prepared separately with the NEBNext® Ultra (tm) II FS DNA Library Preparation Kit and the Unique Dual Indexing kit (New England Biolabs), with reagent volumes reduced to one-fifteenth of that advised by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... was then gel purified using the Qiagen MinElute Gel Extraction Kit (28604) and subjected to Gibson assembly via the New England Biosciences HiFi DNA Assembly Cloning Kit (NEB: E5520S) to insert the amplified region into the hSyn-eGFP vector following its linearization with BamHI (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were qualified on Agilent 2100 Bioanalyzer using High Sensitivity DNA kit and quantified by qPCR using NEBNext Library Quant kit for Illumina (NEB, E7630). Equal molarity of each library were pooled and subjected to sequencing on Illumina HiSeq 4000 and NovaSeq 6000 platforms at the McGill University and Génome Québec Innovation Centre to generate 50 bp paired-end reads.
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bioRxiv - Pathology 2021Quote: ... The kit used for library construction was the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB, USA). Total RNA extracted from the samples was subject to mRNA enrichment using poly-T oligo-attached magnetic beads ...
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bioRxiv - Genomics 2021Quote: ... We individually indexed samples to be pooled within the same sequencing lane during the PCR enrichment step using NEBNext® Multiplex Oligos for Illumina® (single-indexed with E7335 and E7500 kits, or dual-indexed with E6440 kit, New England Biolabs). We performed in-solution hybridization and target enrichment using our 12,049 tiled RNA probes ...
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bioRxiv - Genetics 2021Quote: ... Vector components were purified using the Monarch® DNA Gel Extraction Kit or the Monarch® PCR & DNA Cleanup Kit (NEB) and assemblies were performed using NEBuilder® HiFi DNA Assembly Master Mix (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... Library quality was checked by Agilent BioAnalyzer 2100 using the High Sensitivity DNA kit (Part number 5067-4626) and libraries were quantified using the Library Quant Kit for Illumina (NEB #7630). Libraries were then sequenced using a NextSeq500 platform (75-base-pair (bp ...
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bioRxiv - Molecular Biology 2022Quote: ... Library quality was checked by Agilent BioAnalyzer 2100 using the DNA 1000 kit (Part number 5067-1504) and libraries were quantified using the Library Quant Kit for Illumina (NEB #7630). Libraries were then sequenced using a NextSeq500 platform (75-base-pair (bp ...
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bioRxiv - Biochemistry 2023Quote: ... loci were amplified using the NGSgo®-MX6-1 kit (GenDx) and multiplexed sequencing libraries were prepared using the Ultra™ II FS DNA Library Prep Kit for Illumina (NEB). Libraries were sequenced on a NovaSeq 6000 system (Illumina ...
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bioRxiv - Cell Biology 2023Quote: ... Reverse transcription into cDNA was performed using a cDNA synthesis kit (ProtoScript First Strand cDNA Synthesis Kit, New England BioLabs, Ipswich) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: We constructed NEBNext libraries with 200- and 10-ng RNA by following the guideline of NEBNext Ultra II RNA Library Prep Kit for Illumina kit (NEB, E7770). SmartSeq2 libraries with single cells were prepared following the protocol that was reported by Picelli ...
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bioRxiv - Cell Biology 2024Quote: ... samples were run on a 1% agarose gel and areas corresponding to the expected size were excised and purified from the gel using a gel extraction kit (Monarch DNA Gel Extraction Kit, NEB, USA). This PCR product was used for a second round of amplification followed by gel purification ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was synthesized with RevertAid First Strand cDNA Synthesis Kit (ThermoScientific) reverse transcription kit and PCR was carried out using OneTaq® DNA Polymerase reagents (NEB) using conditions previously detailed and oligonucleotides described in Suppl ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA-seq libraries were generated using the NEBNext Globin&rRNA depletion kit and the NEBNext UltraII Directional RNA Library prep kit according to the manufacturer’s protocols (New England Biolabs, MA, USA), as described previously58 ...
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bioRxiv - Biophysics 2023Quote: ... Vector DNA was linearized with NheI or MluI and cRNA synthesized in vitro using the SP6 or T7 AmpliCap Max High Yield Message Maker Kit (Cellscript, USA) or HiScribe® T7 ARCA mRNA Kit (New England Biolabs) and stored at −20°C (for frequent use ...
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bioRxiv - Molecular Biology 2022Quote: RNA-seq libraries for RIP-TRIBE and polysome were prepared in triplicates from 150 ng of total RNA using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with the NEBnext rRNA depletion Kit (NEB #E6310L). cDNA libraries were sequenced on an Illumina NovaSeq6000 using S-prime or S1 flow cells with paired-end 150-bp reads ...
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bioRxiv - Biochemistry 2022Quote: ... RNA-seq libraries were generated using the NEBNext Globin&rRNA depletion kit and the NEBNext UltraII Directional RNA Library prep kit according to the manufacturer’s instructions (New England Biolabs, MA, USA). Sequencing experiments were performed with NextSeq 500 (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA-seq libraries were generated using the NEBNext Globin&rRNA depletion kit and the NEBNext UltraII Directional RNA Library prep kit according to the manufacturer’s instructions (New England Biolabs, MA, USA). Sequencing experiments were performed using NextSeq 500 (Illumina ...
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bioRxiv - Immunology 2023Quote: ... These amplicons were used as templates for in vitro transcription using the HiScribe T7 mRNA Kit with CleanCap® Reagent AG kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Poly-A selection for mRNA enrichment was performed using the corresponding NEB kit (#E7490) followed by library preparation using the directional RNA library prep kit from NEB (#E7765). An additional NEB kit Low Input RNA library prep kit (#E6420 ...
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bioRxiv - Biophysics 2024Quote: ... Vector DNA was linearized with NheI or MluI and mRNA synthesized in vitro using the SP6 or T7 AmpliCap Max High Yield Message Maker Kit (Cellscript, USA) or HiScribe® T7 ARCA mRNA Kit (New England Biolabs).
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were qualified on Agilent 2100 Bioanalyzer using High Sensitivity DNA Kit and quantified by qPCR using NEBNext Library Quant Kit for Illumina (NEB, E7630). Equal molarity of each library was pooled and subjected to sequencing on Illumina NovaSeq 6000 platform at the McGill University and Génome Québec Innovation Centre to generate 100 bp paired-end reads.
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bioRxiv - Biophysics 2024Quote: ... Vector DNA was linearized with NheI or MluI and cRNA synthesized in vitro using the SP6 or T7 AmpliCap Max High Yield Message Maker Kit (Cellscript, USA) or HiScribe® T7 ARCA mRNA Kit (New England Biolabs) and stored at -20°C ...
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bioRxiv - Developmental Biology 2020Quote: A Fragment Analyzer™ Automated CE System (Analysis Kit: cat.: DNF-474-0500) was used to assess the fragment distributions and a Library Quant Kit for Illumina (NEB, cat.: E7630L) was used to measure molar concentrations ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μg/ml glycogen) and DNA purification was carried out with the PCR cleanup kit (NEB, Monarch PCR & DNA Cleanup Kit, T1030). About 10ng of the purified CUT&RUN DNA was used for preparation of multiplexed libraries with the NEB Ultra II DNA Library Prep Kit per manufacturer’s instruction (NEB #E7103) ...