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Citations for New England Biolabs :
1151 - 1200 of 3865 citations for Borrelia burgdorferi sensu stricto B31 CRASP 1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... 1 U/μL RNase inhibitor (NEB), 1 μM (MetRS) ...
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bioRxiv - Genomics 2023Quote: ... 1 µM Cas9 Nuclease (NEB, M0386S) (2µL ...
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bioRxiv - Cancer Biology 2023Quote: ... 1× CutSmart buffer (New England Biolabs), 1 mM ATP (New England Biolabs) ...
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bioRxiv - Immunology 2022Quote: ... 1 µl Phusion DNA Polymerase (NEB); 2 µl 10 mM dNTPs ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μl β-agarase (NEB M0392S) was added and mixed by flicking without allowing sample to cool ...
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bioRxiv - Biophysics 2023Quote: ... Streptavidin (1 mg/mL, NEB N7021S) was diluted 1:10 in DPBS and applied to passivated flow chambers for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM NAD (New England Biolabs), 2.0 U T5 exonuclease (New England Biolabs) ...
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bioRxiv - Cell Biology 2023Quote: ... 1×Phusion HF Reaction Buffer (NEB),0.2 mM dNTPs (NEB) ...
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bioRxiv - Bioengineering 2023Quote: ... 1× Taq buffer (New England Biolabs), 2 mM manganese(II ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 u XbaI (New England Biolabs), 1.33 u BssHII (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μL DpnI (New England Biolabs), and 5 μL MilliQ water ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL 5′deadenylase (NEB #M0331S), 1 µL of RiboLock (ThermoFisher #EO0381 ...
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bioRxiv - Cancer Biology 2024Quote: ... along with 1 kb (NEB, N3232L) and 100 bp (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... 1 kb plus DNA ladder (NEB)
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL T4 Ligase (NEB, #M0202L), and 1 µL T4 PNK (NEB ...
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bioRxiv - Biophysics 2023Quote: ... and in 1 × CutSmart buffer (NEB). The reaction mixture was pipetted ten times to ensure thorough mixing ...
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bioRxiv - Cell Biology 2023Quote: ... in 1 x GlycoBuffer-2 (Biolabs). After incubation for 60 min at 37 °C ...
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bioRxiv - Bioengineering 2024Quote: ... 1 µL 10 mM dNTPs (NEB), 2 µL each 10 µM forward and reverse primers ...
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bioRxiv - Molecular Biology 2023Quote: ... 1:100 Murine RNase Inhibitor (NEB)) ...
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bioRxiv - Immunology 2023Quote: ... 1 µL RNA ligase I (NEB), 0.5 µL IRDye800-labeled oligonucleotide (34) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... adding 1-2ul ApaLI (NEB #R0507L) and 5ul 10X Cutsmart to 43-ul samples and incubating at 37°C for at least 2 hr (or up to overnight).
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bioRxiv - Plant Biology 2024Quote: ... 1 x cut smart buffer (NEB), and RNase inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... 1 mM dNTPs (New England Biolabs), 3.5 μM of Phi29 Random Hexamer Primer ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µL T4 DNA Ligase (NEB), 100 ng DNA (oligo 3) ...
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bioRxiv - Genomics 2024Quote: 1 μl of Cas9 nuclease (NEB) and 2 μl of single-guide RNA (check concentration ...
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bioRxiv - Microbiology 2024Quote: ... in 1 x rCutSmart buffer (NEB) at 37 °C for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... A pre-cleaning step was applied by incubating each sample with 10 μl of Protein A magnetic beads (New England Biolabs GmbH, Frankfurt, Germany) for 1 h at 4 °C in tube roller (15 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... The DNA encoding the EGFP-fusion proteins and the P2A peptide was inserted by Gibson assembly using NEBuilder HiFi DNA assembly master mix (New England BioLabs, Ipswich, MA, USA). The HeLa Flp-In T-REx K44A Dynamin2-EGFP-P2A-Caveolin1-mCherry construct was obtained by in vitro mutagenesis of the pcDNA/FRT/TO/Dyn2-EGFP-P2A-Caveolin1-mCherry construct exchanging lysine at position 44 to an alanin using these primers ...
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bioRxiv - Developmental Biology 2020Quote: ... A total of 863 Bicyclus embryos were injected with 300 ng/µl of guide and 300 ng/µl of Cas9 protein (NEB; Cat. no.: M0641) mixed together in equal parts (total volume = 10 µl ...
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bioRxiv - Developmental Biology 2019Quote: ... The bound protein (~42kDa, 360 amino acids) was released from the MBP tag by cleavage with Factor Xa (New England Biolabs, Massachusetts, United States) for 24 hours at 4°C in 20mMTris pH 7.2 ...
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bioRxiv - Biochemistry 2021Quote: ... and one plasmid was randomly selected from those plasmids and used for expression with a PURE system (PURExpress In Vitro Protein Synthesis Kit, New England BioLabs, Ipswich, MA, USA).
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bioRxiv - Cancer Biology 2020Quote: ... The synthetic genes encoding WT human TRIM28 with an N-terminal maltose binding protein (MBP) affinity tag (UniProt: Q13263) were subcloned to the pMAL-p2X vector (New England Biolabs, Beverly, MA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Samples were diluted to 1.0 mg/mL protein using homogenization buffer and incubated with 20 U/μL lambda phosphatase in MnCl2 and enzyme buffer as supplied with the lambda protein phosphatase kit (New England Biolabs, Evry-Courcouronnes, France) for 3 h at 30 °C ...
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bioRxiv - Cell Biology 2023Quote: ... corresponding to a portion of PfRNF1 (RP2; Fig. 1A) was expressed as a maltose-binding fusion protein using the pMAL™c5X-vector (New England Biolabs, Ipswich, USA). To this end ...
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bioRxiv - Cell Biology 2022Quote: ... annealed and phosphorylated sgRNA (1:10) and T4 Ligase reaction buffer (1:10, #B0202S, New England Biolabs) with 10 mM 10x Adenosine 5’-Triphosphate (#P0756S ...
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bioRxiv - Cell Biology 2019Quote: ... 1 µl of 1 mM BG-(PEG)12-biotin (New England Biolabs; PEG linker available on request) was added to 200 µl of axonemes ...
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Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery systembioRxiv - Genetics 2019Quote: ... 1 µL of each oligo (100 µM) were mixed with 1 µL of T4 ligation buffer (NEB) and 7 µL of water ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 µl freshly made 1 M NH4HCO3 and calf intestinal alkaline phosphatase (1 U, New England Biolabs) were added ...
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bioRxiv - Molecular Biology 2020Quote: ... Proximity ligation was done under the following conditions: 1 unit/µl RNA ligase 1 (New England Biolabs), 1x RNA ligase buffer ...
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bioRxiv - Genomics 2022Quote: ... and the PCR products were analyzed in 1% agarose with 1 kb DNA Ladder (New England Biolabs). Primers used in this study are shown in Supplemental Table S1.
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bioRxiv - Molecular Biology 2022Quote: ... 1 µg of each pFRT-TO-CLIP-UPF1 mutant vector was digested with 1 µL SpeI (NEB) and 1 µL HindIII-HF (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM EDTA pH 8.0 (Roth) and 1:100 Proteinase K (New England Biolabs 20 mg/ml)) in a 2 ml Eppendorf tube for at least 24 hours at RT in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µl of 1 mM BG-(PEG)12-biotin (New England Biolabs; PEG linker available on request) was added to 200 µl of axonemes ...
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bioRxiv - Microbiology 2023Quote: ... A 1 ml aliquot of each phage was treated with 1 µl DNase (New England Biolabs, USA) at 37 ⁰C for 40 minutes ...
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bioRxiv - Microbiology 2021Quote: The rem allele was amplified from a cloning construct (primer sequences in Table S4) and ligated into pTYB12 (NEB IMPACT system of protein purification), resulting in an N-terminal intein fusion to Rem ...
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bioRxiv - Molecular Biology 2021Quote: ... original numbering with signal sequence = 20–559) were subcloned into the manufacturer-supplied plasmid from the PURExpress® In Vitro Protein Synthesis Kit (New England Biolabs, Ipswich, MA, USA) using the Gibson assembly method with synthetic E ...
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bioRxiv - Microbiology 2021Quote: ... MNase activity was quenched by addition of EGTA and samples were subsequently pre-cleared by addition of 40 µl Protein A Magnetic Beads (New England Biolabs, Ipswich, MA, United States) and rotating at 4°C for 60 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Removal of both N-linked and O-linked glycans was performed by incubation with five different glycosidases: PNGaseF (New England Biolabs, 125 U/μg protein), O-glycosidase (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... aliquots of the same protein extract were separated and treated with 200 U of Lambda Phage Phosphatase (#P0753S, New England Biolabs, Ipswich, Massachusetts, United States), as specified by the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: Injections into fertilized eggs for knockout experiments were done using 50ng/µl of each sgRNA independently and 80nM of NEB EnGen Spy Cas9-NLS protein (New England Biolabs France Genopole, Evry, France), pre-incubated at room-temperature for 15 minutes (Hill et al. ...