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Citations for New England Biolabs :
1151 - 1200 of 2797 citations for 7 Chlorothieno 3 2 b pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... DNA fragments were end-repaired and A-Tailed using Klenow fragment (3’-5-exo-) (NEB, M0212L), the DNA fragments were ligated to illumina adaptors (Illumina ...
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bioRxiv - Molecular Biology 2021Quote: ... 3′ dA-tailed using NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB E7546L) and were purified with paramagnetic beads ...
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bioRxiv - Genetics 2021Quote: ... Singlet and duplet pools were A-tailed using using Klenow HC 3’→5’ exo- (#M0212L; NEB).
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bioRxiv - Cancer Biology 2020Quote: ... Positive control slides were treated with 3 U/mL DNase-1 (New England Biolabs, Cat. M0303) for 10 minutes at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... and a NEBNext Multiplex Oligos for Illumina (Index Primers Set 3) (Code E7710; New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... denatured and phosphorylated with 10 U of 3’-phosphatase-minus T4 polynucleotides kinase (New England Biolabs) at 37 °C for 30 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A deoxyadenosine triphosphate was added at each 3’end with the Klenow fragment (New England Biolabs), and (3 ...
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bioRxiv - Neuroscience 2023Quote: ... 3 μg of total RNA was reverse transcribed using LunaScript cDNA Synthesis Kit (New England Biolabs). Gene-expression levels were quantified by real-time quantitative PCR (iCycler iQ BioRad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... A deoxyadenosine triphosphate was added at each 3’end with the Klenow fragment (New England Biolabs). Illumina Truseq adapters (Affymetrix ...
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bioRxiv - Molecular Biology 2023Quote: ... 3-4 μg of sperm gDNA was dephosphorylated with 10 μl of rSAP (NEB, no. M0371S) and 16 μl of 10× rCutSmart buffer (NEB ...
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bioRxiv - Genetics 2023Quote: ... then dATP was added to the 3′ ends of the DNA using Klenow exo-(NEB #M0212). The DNA was then subjected to double-sided SPRI bead size selection (AMPure XP beads ...
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bioRxiv - Microbiology 2023Quote: ... Pre-adenylated L3-1R-App 3’ adaptors were ligated using T4 RNA ligase 1 (M0204, NEB) for 75 min at 25°C ...
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bioRxiv - Biophysics 2022Quote: ... The gene sequences were flanked by restriction enzyme sites for 5’ NdeI and 3’ EcoRI (NEB). Genes were cloned into pET21a using standard protocols from NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’ and 3’ homology arms and the insert were cloned into the pUC19 vector (NEB, #N3041S) by using Gibson Assembly Master Mix (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Buffer IIIb (3% Triton X-100, 1x T4 ligase buffer, 1x protease inhibitors; NEB, Sigma-Aldrich) was added to a final volume of 1 ml and sample was incubated for 15 min at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... newly deposited CENP-A was labelled with 3 µM SNAP-Cell® 647-siR (S910102S, NEB) for 30 mins before washing excess with media and allowing to grow for a further 30 mins ...
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bioRxiv - Molecular Biology 2024Quote: ... About 3 µg of DNA from each sample was digested with two restriction enzymes (PstI; NEB catalog #R0140 and XhoI ...
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bioRxiv - Bioengineering 2024Quote: ... The extracted DNA (3 μg) was fully digested with NcoI restriction enzyme (New England Biolabs, USA), separated in a 0.8% agarose gel and blotted onto a Hybond+ Nylon membrane (Amersham Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... or 2 μL of Shortcut RNase III (0.02 L/μL; NEB) were added and incubated for 45 seconds ...
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bioRxiv - Genomics 2020Quote: ... with 2 μl (5 U/μl) of Klenow fragment exo- (NEB) in a final volume of 55 μl by incubation at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were resuspended in 2% bovine serum albumin (New England BioLabs) and were transferred immediately in ice to the genomics core laboratory.
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bioRxiv - Molecular Biology 2021Quote: ... 2 µg BSA dealt as negative control (NEB, Ipswich, MA, USA). Samples were applied in a final volume of 100 µl coating buffer (100 mM Tris-HCL pH 8 ...
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bioRxiv - Bioengineering 2022Quote: ... 1 μL of 2-Log DNA Ladder (200 μg/mL; NEB) is mixed with 1 μL of Gel Loading Dye (6x ...
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bioRxiv - Genomics 2019Quote: ... 10 µL T4 RNA ligase 2 truncated (200 U/µL, NEB) was added ...
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bioRxiv - Systems Biology 2020Quote: ... and 2 μL NEBNext Second Strand Synthesis Enzyme Mix (E6111S, NEB) were added to the purified cDNA ...
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bioRxiv - Cell Biology 2019Quote: ... 2 µL of T4 RNA ligase II truncated KQ (NEB M0373), 0.5 µL of SUPERaseIN ...
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bioRxiv - Cell Biology 2021Quote: ... and digested with 2 U/μl of DpnII restriction nuclease (NEB) at 37 °C overnight ...
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bioRxiv - Immunology 2021Quote: ... a mix containing 2 μl of RNAse H (NEB, Cat. #M0297S), 1 μl of E ...
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bioRxiv - Genomics 2021Quote: ... the reaction was incubated with 2 μL of USER (NEB #5508) in 1X CutSmart Buffer at 37 °C for 15 min before final purification with Zymo Oligo Clean & Concentrator kit ...
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bioRxiv - Synthetic Biology 2021Quote: ... SARS-CoV-2 RNA was replaced with nuclease free water (NEB) for the assay without the target RNA ...
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bioRxiv - Microbiology 2020Quote: ... 12.5 μl Q5 high-fidelity 2*master mix (New England Biolabs), 8.5 μl dH2O ...
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bioRxiv - Genomics 2021Quote: ... 2 μL of 1 mg/ml bovine serum albumin solution (NEB), 1 μL of hOGG1 (ProSpec TechnoGene Ltd. ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10 units/µL T4 RNA Ligase 2 (truncated K227Q, NEB). The ligation reaction was incubated for 3 hours at 22 °C ...
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bioRxiv - Microbiology 2020Quote: ... after which 2 µL calf intestinal phosphatase (10,000 U/mL, NEB) was added to the digest and incubated for 1h at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... and 4 μl T4 RNA ligase 2 (NEB, catalog no. M0239) for 16 hours at 16°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 800U of truncated T4 RNA ligase 2 K227Q (New England Biolabs) in supplied PNK buffer with RNAsin (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... and RNA ligase 2 of bacteriophage T4 (New England BioLabs, Inc.). Reactions were incubated for one hour at 25 °C and ligated duplexes were purified by 1ml monoQ column (Cytiva ...
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bioRxiv - Microbiology 2022Quote: ... An additional 2 μl RNase A (20 mg/ml, NEB #T3018) was added and followed by 5 min incubation at 56 °C to remove residual RNA in the sample ...
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bioRxiv - Plant Biology 2021Quote: ... in 1X NEB Buffer 2 (New England BioLabs; Ipswitch, MA, USA), and 0.1 mM dATP for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... 1–2 mg of RNA was treated with DNAse I (NEB), x µL and 2 µL of this reaction was used to make cDNA ...
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bioRxiv - Molecular Biology 2020Quote: ... After the addition of 50ul of 10X NEBBuffer 2 (NEB, B7002) and 1ul of MboI restriction enzyme (NEB ...
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bioRxiv - Genomics 2020Quote: ... 2 μL 5x Q5 reaction buffer (New England Biolabs, Ipswich, MA), 0.2 μL 10 mM dNTPs (Kapa Biosystems ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 µl NEB-Next High-Fidelity PCR Mix (NEB, cat # M0541S), 5 µl of SYBR Green (Invitrogen™ ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 2 μl of the rare-cutter AseI (New England Biolabs, UK) was added and incubated at 30 °C overnight ...
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bioRxiv - Biophysics 2019Quote: ... The annealed strands were ligated with T4 RNA ligase 2 (NEB), in 20 μl reactions for 1h at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... 2 μl of 10X T4 RNA Ligase Buffer (New England Biolabs), 1 μl of 2 μM 5’-adenylated RNA linker ...
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bioRxiv - Genomics 2021Quote: ... 50 μL NEB Buffer 2 and 375 U MboI (NEB R0147M) were added and allowed to incubate for 2 hours at 37°C rotating ...
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bioRxiv - Biochemistry 2021Quote: ... 400 μM ATP (1x T4 RNA ligase 2 reaction buffer (NEB)) including 0.25 units/μL T4 RNA ligase 2 (Neb ...
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bioRxiv - Microbiology 2021Quote: ... Ligation was conducted using T4 double stranded RNA ligase 2 (NEB) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Oligos (2 μM each) were annealed in 1X CutSmart buffer (NEB) at 95°C for 5 min followed by cooling to room temperature ...