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Citations for New England Biolabs :
1101 - 1150 of 10000+ citations for Mouse Acrosomal Protein SP 10 ACRV1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The purified protein was labeled with SNAP-Surface Alexa Fluor647 dye (New England Biolabs). The fractional dye labeling and DNA binding activity were assessed as previously described [12].
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined via SDS–PAGE and Coomassie staining using BSA (NewEngland Biolabs) as a standard ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then degraded by addition of 1 μL of Proteinase K (NEB #P8107) and incubation at 56°C for 30 min ...
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bioRxiv - Immunology 2023Quote: For embryo microinjection the annealed sgRNA was complexed with Cas9 protein (New England Biolabs) at room temperature for 10 minutes ...
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bioRxiv - Physiology 2023Quote: ... for every 2 µg of fusion protein in 1X TEV buffer (Catalog P8112S, NEB) for 16h at 30°C.
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bioRxiv - Physiology 2023Quote: ... The proteins were then purified by affinity chromatography using amylose resin (Catalog E8021L, NEB). The amylose resin was washed and pre-equilibrated in 20 mM tris ...
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bioRxiv - Cell Biology 2023Quote: ... MBP-tagged CCDC22 and CCDC93 proteins were purified using Amylose beads (New England Biolabs), mixed in approximately 1:1 stoichiometry ...
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bioRxiv - Developmental Biology 2023Quote: ... Injection mixtures containing 500 ng/µl of Cas 9 protein (NEB; Cat. no.: M0641) and 300 ng/µl of guide RNA were prepared and injected into eggs within 4 hours of egg laying ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were purified on amylose resin (New England Biolabs, Ipswich, MA, USA; Cat# E8021) and eluted with maltose using the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... immunoprecipitated beads were incubated with 40 Units of lambda protein phosphate (New England Biolabs) in a 50 μl reaction ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg of lysates were deglycosylated using Protein Deglycosylation Mix II (NEB Cat# P6044S). In short ...
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bioRxiv - Immunology 2023Quote: ... with a mixture of 0.33 µM EnGen Cas9 protein (New England Biolabs, Cat.No. M0646T), two Cas9 guides RNA ...
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bioRxiv - Cell Biology 2023Quote: ... cleaved protein product was passed through Ni-NTA resin and amylose resin (E8021, NEB) to remove the TEV protease and HIS-MBP tag ...
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bioRxiv - Biochemistry 2024Quote: ... Bound proteins were eluted by digestion with 500 units micrococcal nuclease (New England Biolabs) for 10 minutes at 37°C in 25 mM HEPES-KOH (pH 7.6) ...
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bioRxiv - Biochemistry 2024Quote: ... The proteins were purified on the amylose resin (New England Biolabs, Ipswich, MA, USA) and stored at −20 °C in 25 % glycerol ...
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bioRxiv - Genomics 2022Quote: ... and ribosomal RNAs were depleted using ribosomal RNA depletion kit (rRNA Depletion Kit, NEB). Ribosomal depleted RNA was used to make the RNAseq libraries using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid mini-prep kit and PCR DNA clean-up kits were provided by NEB.
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bioRxiv - Genomics 2020Quote: ... The supernatant was loaded on a 10-mL chitin column (NEB). The column was washed with HEGX ...
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bioRxiv - Biochemistry 2022Quote: ... 10 u/μL T4 DNA ligase (stock 400 u/μL, NEB). No difference in yield was found between the ligation splints ...
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bioRxiv - Molecular Biology 2021Quote: ... were then combined with 10 μM CLIP-Biotin (New England Biolabs) and rotated (end-over-end ...
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bioRxiv - Developmental Biology 2022Quote: ... in HBSS supplemented with 10 µg/ml DNase (New England Biolabs) at 37°C and swirled at 100 rpm for 32 minutes to enzymatically separate the epithelium from the mesenchyme as we previously did [47] ...
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bioRxiv - Genomics 2020Quote: ... 5 μL of 10 U/μL T4 PNK (New England Biolabs), 4 μL of 3 U/μL NEB T4 DNA Polymerase (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... and 3’-end dephosphorylated by 10 U T4-PNK (M0201S, NEB) in the presence of 20 U RNase inhibitor (M0314L ...
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bioRxiv - Genomics 2019Quote: ... 10 µL T4 RNA ligase 2 truncated (200 U/µL, NEB) was added ...
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bioRxiv - Microbiology 2019Quote: ... with the addition of 10 μg of BSA (New England BioLabs). The PCR reaction was incubated at 95°C for 3.5 minutes with 30 cycles of 30 s at 95.0°C ...
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bioRxiv - Genetics 2020Quote: ... supplemented with 10 μl Proteinase K (800 U/ml, NEB #P8107), and incubated at 55°C for 14-18h while shaking at 800rpm ...
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bioRxiv - Cancer Biology 2019Quote: ... 10 μL of 400 U/μL T4 DNA Ligase (NEB, M0202), and 660 μL of water ...
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bioRxiv - Microbiology 2019Quote: ... 10 uL NEBNext Q5 Hot Start HiFi Master Mix (NEB M0543L), 0.2X final concentration SYBR Green I ...
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bioRxiv - Developmental Biology 2020Quote: ... and transformed into NEB 10-beta Competent E.coli (NEB, Ipswich, MA). Colonies positive for the Lgals3-R200S allele by PCR were grown overnight at 37 °C with nutation at 200 rpm ...
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bioRxiv - Genomics 2019Quote: ... 10 µL of 400 U/µL T4 DNA Ligase (NEB, M0202), and 660 µL of water ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 µL of 400 U/µL T4 DNA Ligase (NEB, M0202), and 660 µL of water ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 µM Rad51 and 10 µM (nucleotide) φX174 circular ssDNA (NEB) were mixed in reaction buffer C (30 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 10 μl NEBNext 2x High-Fidelity Master Mix (New England Biolabs), 0.3 μM of each primer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... coli K-12 DH10B (Neb 10-beta, NEB catalogue no. C3019H), E ...
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bioRxiv - Microbiology 2020Quote: ... and the assembly reaction used to transform E.coli (NEB 10 beta). Colonies positive by PCR screening using primers that flank the cloning site were sequenced across the entire S coding region and a single positive isolate adopted for all further manipulations.
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bioRxiv - Molecular Biology 2021Quote: ... and 10 units/µL T4 RNA Ligase 2 (truncated K227Q, NEB). The ligation reaction was incubated for 3 hours at 22 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 µL of the PhD library (1011 pfu, New England Biolabs) is added onto dispersed flakes and incubated for 3 hours on a rotator at room temperature and then washed twice before overnight incubation ...
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bioRxiv - Neuroscience 2022Quote: ... mixed with 3 µL 10% SDS and 1.8U Proteinase K (NEB), and incubated for 1 hour at 50°C ...
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bioRxiv - Biochemistry 2022Quote: ... the plasmid coding for segment 10 was linearized with BsaI (NEB), and the plasmids coding for the remaining segments were linearized using BsmBI (NEB) ...
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bioRxiv - Cell Biology 2022Quote: ... with 10 mM 10x Adenosine 5’-Triphosphate (#P0756S, New England Biolabs) for 30 min in RT.
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bioRxiv - Plant Biology 2021Quote: ... 1 μL 10 mM ATP (New England BioLabs; Ipswitch, MA, USA), 10 units T4 RNA Ligase 1 (New England BioLabs ...
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bioRxiv - Microbiology 2021Quote: ... and 10 U/mL of M-MuLV Reverse Transcriptase (NEB, M02553L) was added to each sample and incubated for 1 hr at 42°C ...
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bioRxiv - Microbiology 2019Quote: ... and 10 pmol of thermostable 5’ App DNA/RNA ligase (NEB) in 10-μl reaction volume ...
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bioRxiv - Developmental Biology 2020Quote: ... and 10 mM ribonucleoside vanadyl complex (RVC; New England Biolabs, S1402S) for 10-20 minutes in a 37°C water bath ...
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bioRxiv - Microbiology 2021Quote: Escherichia coli (NEB® 10-beta Electrocompetent E. coli or NEB® 5-alpha Competent E ...
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bioRxiv - Biophysics 2019Quote: ... 70 µL Millipore water and 10 µL T4 DNA ligase (NEB) were added to the hybridization reaction and incubated for 60 min at 20 °C.
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bioRxiv - Genomics 2021Quote: ... mixed with 3 μL 10% SDS and 1.8U Proteinase K (NEB), and incubated for 1 hour at 50°C ...
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bioRxiv - Biochemistry 2021Quote: ... Nb35–His (10 μg/mL) and apyrase (25 mU/mL, NEB); the suspension was incubated for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Nb35–His (10 µg/ml) and apyrase (25 mU/ml, NEB). The suspension was incubated for 1 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 mM ribonucleoside vanadyl complex (New England Biolabs, Ipswich, MA), 10 minutes in 4% paraformaldehyde (Ted Pella ...