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Citations for New England Biolabs :
1101 - 1150 of 4087 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and 5′-hydroxyl group repair (T4 polynucleotide kinase (M0201L; NEB) were conducted on beads ...
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bioRxiv - Developmental Biology 2023Quote: ... Protruding 5’ ends were filled in with Klenow enzyme (NEB) in the presence of [P]-32 dCTP (Hartmann Analytics ...
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bioRxiv - Evolutionary Biology 2022Quote: ... coli in 5 x 25 uL cell aliquots (NEB #C3020). Each cell aliquot was recovered in 1 mL outgrowth media at 37°C for 1 hr and then transferred into 100 mL of molten LB (1% tryptone ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 µL RNase H enzyme (5 U) (New England Biolabs) was added and the solution was incubated at 44°C for 1 hr ...
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bioRxiv - Genomics 2023Quote: ... 5 μL of rSAP mix (rSAP (1 U, NEB, M0371L) in 1X CutSmart buffer (for MseI/NdeI digestions ...
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bioRxiv - Microbiology 2022Quote: ... 2.5 μL of Klenow Fragment (3’ -> 5’ exo -, NEB M0212L), 5 μL 50% PEG 8000 ...
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bioRxiv - Cell Biology 2023Quote: ... NEB 5-alpha competent E.coli (NEB #C2987H, Ipswich, MA, USA) were transformed with ligated plasmid by heat shock at 42°C for 30 seconds ...
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bioRxiv - Cell Biology 2023Quote: ... using Large Klenow fragment 3’-5’ exo- (New England Biolabs). Biotinylated 19x 601 array DNA ...
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bioRxiv - Genomics 2023Quote: ... by using Klenow Fragment (3′→5′ exo-) (New England Biolabs) for 30 min at 37 °C and purified by using a QIAquick PCR Purification Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... Linker reactions were removed with 5′ deadenylase (New England Biolabs) and Rec J Exonuclease (NEB) ...
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bioRxiv - Physiology 2024Quote: ... 5 μL of α1-2,4,6 fucosidase O (2U/μl, NEB) with 1x GlycoBuffer 1 (NEB).
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bioRxiv - Plant Biology 2024Quote: ... 5 and 7dpi using a total RNA extraction kit (NEB) and following the kit instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Five μl of 5 U/μl Klenow fragment (NEB M0210L) was added to achieve a final concentration of 0.5 U/μl and incubated at 37°C for 15 min with the same agitation program as the previous step ...
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bioRxiv - Plant Biology 2024Quote: ... and A-tailed using Klenow fragment (3’-5’ exo-; NEB). The truncated Illumina Y-adapter was ligated to the DNA using T4 DNA ligase (Promega).
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bioRxiv - Molecular Biology 2024Quote: ... 5 μL T4 Polynucleotide Kinase Reaction Buffer (NEB, Cat #B0201S) and 5 μL 10 mM ATP ...
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bioRxiv - Genomics 2024Quote: ... 1 μL BST DNA Polymerase FL (NEB, 5 U/µL), 2μL TAQ Ligase (NEB ...
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bioRxiv - Immunology 2024Quote: ... and 5 μL of Proteinase K (NEB catalog no. P8107S). Tubes were incubated for 15 min at 37 °C with shaking at 600 rpm ...
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bioRxiv - Microbiology 2024Quote: ... 5 μl inorganic pyrophosphate (New England Biolabs product number M2403), and ultrapure water (as needed ...
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bioRxiv - Genomics 2024Quote: ... and dTTP with 5 U/µL DNA polymerase (NEB # M0210), the DNA was ligated by T4 DNA ligase (NEB # M0202 ...
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bioRxiv - Systems Biology 2024Quote: ... 1 μL of Klenow DNA Polymerase (5 U/μL, NEB) and 2 μL of T4 PNK (10 U/μL ...
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bioRxiv - Genomics 2024Quote: ... and 5 µl DNA Polymerase I (Large Klenow Fragment) (NEB). The reaction was incubated for 60 min at 37 °C and subsequently purified and eluted in 13 µl ddH2O using a MinElute kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: Chemically competent NEB® 5-alpha F’Iq cells (NEB; C2992H) were transformed with recombinant plasmids per manufacturer instructions and grown at 37°C on selective Luria-Bertani (LB ...
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bioRxiv - Genomics 2024Quote: ... and 3 μl of 5′-deadenylase (NEB, Catalog no. M0331S) to the 60 μl end-prepped product ...
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bioRxiv - Bioengineering 2024Quote: ... 5’-end labeling was performed using T4 Polynucleotide Kinase (NEB) and [gamma-32P] ATP (PerkinElmer) ...
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bioRxiv - Bioengineering 2024Quote: ... 5 μL of T4 DNA ligase (NEB, 400000 U/mL), and 10 μL of 10X T4 DNA ligase buffer (NEB) ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μl H2O and 1 μl PNGase F (NEB, P0704S) were then added to the lysate ...
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bioRxiv - Bioengineering 2024Quote: ... then 5 μL of 6X Purple Loading Dye (NEB, B7024S) were added and run on a GelRed-stained 1% (w/v ...
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bioRxiv - Genomics 2022Quote: ... 2 μl of 10X CutSmart® buffer (1X final) and 2 μl of 1U/μl USER® II enzyme (M5505S, NEB) (0.1U/μl final ...
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bioRxiv - Microbiology 2020Quote: ... stained with a mAb cocktail composed of SARS-CoV-2 spike (Creative-Bios; 2BCE5) and SARS-CoV-2 nucleoprotein (Creative-Biolabs; NP1C7C7) followed by anti-Mouse IgG-HRP (Abcam ab6823 ...
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bioRxiv - Genomics 2021Quote: ... and supernatant was transferred to a new tube and added to 2 μL 10% SDS and 2 μL 20 mg/mL proteinase K (New England Biolabs P8107). Samples were incubated overnight at 65°C to reverse crosslinks ...
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bioRxiv - Genetics 2023Quote: ... 5 µl of 100 ng/µLcDNA diluted from the previous step was combined with 2 µL of block mix and 2 µL of nuclease free water (NEB, AM9937), and then the cDNA block oligo mix was incubated on a thermocycler under the following conditions to allow block oligo mix to bind to the 5′ end and the 3′ end of the cDNA molecule ...
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bioRxiv - Microbiology 2023Quote: ... antibiotic resistance cassette and 2 flanking regions were combined in equimolar amounts and mixed with 2 x HiFi reagent (NEB, UK) and incubated at 50°C for 1 hour ...
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bioRxiv - Synthetic Biology 2021Quote: ... for 4 hours at 37°C and treated with Antarctic phosphatase (NEB) for 30 min at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Annealed oligos were mixed with 4 μL 6X loading dye (NEB, B7024S), loaded onto a lab-made 8% TBE gel (Acrylamide/Bis solution 37.5:1 ...
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bioRxiv - Genomics 2020Quote: ... the circularized DNA was incubated in 1x NEBuffer 4 reaction buffer (NEB) (50 mM potassium acetate ...
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bioRxiv - Systems Biology 2020Quote: ... a 4-cycle PCR was performed with OneTaq polymerase (New England Biolabs) in 200 reactions (125 μL/reaction) ...
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bioRxiv - Microbiology 2021Quote: ... The parental plasmid was digested for 4 h using Dpn1 endonuclease (NEB) at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... a mix of 4 μl First strand Synthesis Reaction Buffer (NEB-kit), 0.5 μl Murine RNase Inhibitor (NEB-kit ...
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bioRxiv - Genomics 2020Quote: ... 4 μL of 20 mg/mL RNase A (New England Biolabs #T3018L) is added to the RNase positive aliquot of non-lysed TSB from 1-gram cannabis flower homogenate ...
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bioRxiv - Developmental Biology 2021Quote: ... by incubating with DNA polymerase I Klenow (4 mL; New England Biolabs) for 90 min at 37C with rotation ...
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bioRxiv - Immunology 2021Quote: ... Step 3 was cloned into Step 4 with T4 DNA ligase (NEB) and then this recominbeering fragment (5’ arm of homology ...
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bioRxiv - Synthetic Biology 2022Quote: ... 4 uL BbsI-HF (NEB R3539L, 1 uL T4 ligase (NEB M0202M), and 1uL of an XTEN linker-encoding DNA amplicon ...
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bioRxiv - Synthetic Biology 2022Quote: ... 4 uL BbsI-HF (NEB R3539L, 1 uL T4 ligase (NEB M0202M), and 1uL of an XTEN linker-encoding DNA amplicon ...
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bioRxiv - Genetics 2023Quote: ... 4 µl Luna universal qPCR Master Mix (New England Biolabs, Hitchin, UK) and 1.8 µl ultrapure water ...
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bioRxiv - Developmental Biology 2024Quote: ... 47 ml water) and 4 µL 10mg/ml Proteinase K (P8108S, NEB). The samples were incubated for 8 hours at 55 °C followed by 10 min at 98 °C to inactivate the Proteinase K ...
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bioRxiv - Biochemistry 2024Quote: ... then subsequent addition of 4 units of proteinase K (New England Biolabs) and incubation at 55 °C for 30 min ...
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bioRxiv - Biophysics 2024Quote: ... 4 mM BME) and added to an amylose resin (New England Biolabs), incubating overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... and subsequently incubated with 4 U of alkaline phosphatase (New England Biolabs) at 37°C for 30 min to hydrolyze unreacted NTPs ...
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bioRxiv - Cell Biology 2024Quote: ... 40U (4 µL) T4 polynucleotide kinase enzyme (T4 PNK; NEB, Cat #M0201S), 4 µL 10X T4 Ligase Buffer (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... Gibson assembly was performed by combining a part amplicon and linearized pEmpty in a 2:1 mass ratio in 2× NEB Gibson Assembly Master Mix (NEB, Cat# E2611S) and incubating at 50℃ for 2 hr before transformation ...