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Citations for New England Biolabs :
1051 - 1100 of 1195 citations for Recombinant Human PVRL4 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... GST fusion proteins of the CTD and its mutants CTD-AA and CTD-YA were phosphorylated by Cdc2 kinase (NEB) using 32P-γ-ATP for 30 minutes at 30°C before incubated with immunoprecipitated CoAA or hnRNP M ...
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bioRxiv - Biophysics 2019Quote: ... was generated as a fusion construct with N-terminal maltose binding protein (MBP) as we described previously (45) using the pMAL-c2x (New England Biolabs) variant pMALX (46) ...
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bioRxiv - Cell Biology 2019Quote: SNAPf–tagged Kinesin-8X-MD proteins (20 μM) were biotinylated in 50 μl reaction volumes by incubating with 40 μM SNAP-biotin (NEB) at 4 °C for 1.5 hrs ...
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bioRxiv - Plant Biology 2019Quote: The nucleic acid sequences encoding the mature GUN1 and RH50 proteins were cloned into the BamHI-SalI sites of the pMAL-c5X Vector (New England Biolabs). In both cases ...
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bioRxiv - Cell Biology 2019Quote: ... To visualize SNAP-tagged dCENP-A proteins cells were labelled with 3 µM SNAP-Cell TMR Star (New England Biolabs) for 30 min ...
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bioRxiv - Biochemistry 2019Quote: ... Those fractions containing the protein of interest were pooled and incubated 30 minutes at 4°C with pre-equilibrated amylose resin (New England BioLabs) and eluted with elution buffer (30 mM HEPES pH 8.0 ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... to a final concentration of 0.1µg/ml was incubated with 3 µg purified STAT3 proteins as well as 5 µl ATP (New England Biolabs; N0440S) for 30 minutes at 30°C ...
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bioRxiv - Neuroscience 2020Quote: ... human parkin proteins were expressed as 6His-Smt3 fusion proteins in Escherichia coli BL21 (DE3) Codon-Plus RIL competent cells (C2527, New England Biolabs) as previous described [40–42] ...
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bioRxiv - Microbiology 2020Quote: ... fusion protein was purified by affinity solid phase extraction on an amylose resin as suggested by the manufacturer (New England Biolabs). Rabbits were immunised by intramuscular injections of 400 μg purified MBP–A104R fusion protein every four weeks over a period of four months ...
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bioRxiv - Microbiology 2021Quote: ... and cloned to the C terminus of a 11 His-tagged maltose-binding protein in a pMAL-C5X vector (New England Biolabs) separated by a cleavage site for tobacco etch virus protease ...
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bioRxiv - Biochemistry 2020Quote: Ten micrograms of purified RBD proteins were treated by 500 units of PNGase F (New England Biolabs, Ipswich, USA #P07045) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2019Quote: ... meliloti 2011 strains containing the plasmid pRK-MS2-rnTrpL was performed using MS2-MBP fusion protein bound to amylose beads (New England Biolabs) as described by Smirnov et al ...
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bioRxiv - Neuroscience 2019Quote: ... Heated mixtures were cooled to room temperature for 10 min and 30 ng/µl EnGen Cas9 protein (New England Biolabs) was added ...
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bioRxiv - Biochemistry 2019Quote: ... Reactions were carried out in 10 μl of the purified components from the PURExpress In Vitro Protein Synthesis Kit (E6800S/L, NEB) with the corresponding templates ...
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bioRxiv - Biochemistry 2019Quote: ... In the case of Biotinylated-AviTag-haBiP(28-635)T229A-V461F this protein was also made nucleotide free by the addition of 2 U CIP (NEB) per mg of BiP ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mM of purified protein was combined with 2 mM DTT and 10 mM SNAP-Surface Alexa Fluor 546 (New England Biolabs) in PBS for 60 min at 30°C ...
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bioRxiv - Biochemistry 2020Quote: ... eluted protein samples may be treated with TEV protease at 4 °C overnight before flowing through Amylose resin (New England Biolabs) for a second step of affinity purification ...
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bioRxiv - Neuroscience 2020Quote: ... the enhanced green fluorescent protein sequence and an SV40 polyA) was blunted at both ends using a Klenow fragment of DNA polymerase I (NEB) and cloned into the EcoRV site of the pminiTol2 plasmid (ref) ...
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bioRxiv - Genomics 2021Quote: ... equal amount of naked DNA and DNA bound by Reb1 protein (~ 5pmol) were incubated with AAG (10 units) and APE1 (1 unit) (New England Biolabs) in a 20 μL reaction containing 1X Thermopol buffer (20mM Tris HCl pH 8.8 ...
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bioRxiv - Immunology 2020Quote: For recombinant CARD proteins, NLRP1 constructs (985-1473, 1213-1473, 1374-1466, 1374–1473) with or without C-terminal SNAP tag (NEB), CARD8–CARD (451-537 ...
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bioRxiv - Immunology 2020Quote: ... The HDR templates were mixed at fourfold molar excess with a single-strand DNA binding protein (ET SSB, NEB, M2401S) and heated at 95°C for 10 minutes to achieve singlestranded donor oligonucleotides (ssODNs ...
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bioRxiv - Microbiology 2021Quote: pTXB1 and pTYB21 derivatives for protein overproduction were constructed based on the guidelines provided with the IMPACT kit (New England Biolabs). The coding sequences of nepR1 ...
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An egg-derived sulfated N-Acetyllactosamine glycan is an antigenic decoy of influenza virus vaccinesbioRxiv - Immunology 2021Quote: ... 25 ug of the 2020 QIV was denatured for 10 minutes at 75°C and treated with the Protein Deglycosylation Mix II (New England Biolabs) for 30 minutes at 25°C and 1 hour at 37°C ...
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bioRxiv - Immunology 2020Quote: ... The resulting expression vectors were confirmed for correct fusion protein open reading frames by sequencing and each was linearized with PmeI (New England Biolabs) and transformed into the P ...
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bioRxiv - Immunology 2022Quote: Protein expression plasmids were constructed using dsDNA gBlocks from IDT and NEBuilder® HiFi DNA Assembly Cloning Kit (NEB, E5520S) and then transformed in NEB 5-alpha competent cells (NEB ...
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bioRxiv - Microbiology 2022Quote: ... following which the beads were washed three times with IP buffer and the bound proteins eluted with 30 μL 3x Sample Loading Buffer (NEB) at 95 °C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... following which the matrix was washed three times with IP buffer and the bound proteins eluted with 30 μL 3x Sample Loading Buffer (NEB) at 95 °C for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... Proteins from the supernatant at 2 mg/mL were used for the Co-IP assay using Protein G magnetic beads (New England Biolabs) and a mouse monoclonal anti-HA antibody (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 nM RNAse-free DNA fragments containing gRNA target sequences and 30 nM Cas9 or Cas12a protein (NEB, Ipswich, MA) were mixed in reaction tubes as per manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... DNA templates are prepared by inserting protein-coding sequence of gLuc (pCMV-GLuc control vector, New England BioLabs, Ipswich, MA) into pSP73 vectors (Promega ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Fusions to the miniGs-399 protein with C-terminal truncations to RXFP1 were constructed using PCR followed by NEBuilder HiFi DNA Assembly (New England Biolabs). Truncations removed 10 ...
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bioRxiv - Biochemistry 2024Quote: ... a pET-derived vector that is normally used for T7 RNA polymerase-dependent protein expression (AVA421) was linearized by digestion with XbaI (NEB) in a reaction containing 1X CutSmart buffer and 0.5 U/μl XbaI to be used for run-off transcription using T7 RNA polymerase to transcribe the first 26 nucleotides following the T7 promoter sequence to generate the standard model RNA substrate ...
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bioRxiv - Bioengineering 2024Quote: ... and mCherry inserts were generated via PCR and cloned into the linearized viral backbone as a single fusion protein using HiFi cloning mix (NEB). H2B-iRFP nuclear marker was (Addgene Plasmid #90237) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 40 – 50 µg of protein lysate from each sample was denatured in 1X Glycoprotein Denaturing Buffer (Cat.#B1704S; New England Biolabs) with 20 µL total volume at 100°C for 10 min ...
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bioRxiv - Genomics 2024Quote: Each of the fragmented protein factor-enriched chromatin sample was mixed with 50 µg/ml of BSA (cat# B9000S, NEB) to prevent chromatin aggregation ...
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bioRxiv - Microbiology 2024Quote: ... reactions were performed in a final volume of 100 μl using the PURExpress ΔRibosome In vitro Protein Synthesis Kit (New England Biolabs) according to the manufacturer instructions and with ribosomes purified from E ...
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bioRxiv - Molecular Biology 2023Quote: ... Template DNA for in vitro protein synthesis was generated with Phusion® Hot Start Flex DNA Polymerase (NEB. Ipswich MA) using gBlocks as template and flanking primers (Supplementary Table S2) ...
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bioRxiv - Neuroscience 2022Quote: ... 100 cells were isolated directly into low protein binding eppendorfs containing 2 ul NEBNext Single Cell Lysis Buffer (NEB, E5530S). Samples were kept on dry ice until transfer to −80 C for overnight storage.
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bioRxiv - Biochemistry 2022Quote: ... and LaTR were fused to the C-terminus of the maltose binding protein (MBP) in the pMAL-c2x vector (New England Biolabs). Precisely ...
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bioRxiv - Genomics 2022Quote: ... for 2 hours and then crosslinked with p19 siRNA binding protein (1 μg/ml in depc-PBS; New England Biolabs) or anti-N6-methyladenosine (m6A ...
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bioRxiv - Microbiology 2023Quote: ... The eluted gp120 coree protein was deglycosylated overnight in a 37 °C water bath with Endoglycosidase Hf (New England Biolabs). Afterwards ...
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bioRxiv - Microbiology 2023Quote: Reporter fusions of sRNA targets were in-vitro translated in the absence and presence of sRNA using the PURExpressⓇ In Vitro Protein Synthesis kit (NEB). Four picomoles of in-vitro transcribed 5’UTR reporters (including the RBS/sRNA interaction site and the first 10 codons of flgM ...
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bioRxiv - Biochemistry 2023Quote: ... four cycles of RD were performed using the PureExpress in vitro protein synthesis kit (New England Biolabs, Ipswich, MA, USA) for in vitro transcription and translation ...
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bioRxiv - Cancer Biology 2023Quote: ... lysate volume equivalent to 20-40 µg total protein was first denatured at 100°C for 10 min in 1X glycoprotein denaturation buffer (NEB). Endo H digestion was performed at 37°C for 1h in the presence of 1X GlycoBuffer 3 and 1 µL of Endo H enzyme (NEB) ...
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bioRxiv - Molecular Biology 2023Quote: ... with GFP protein fused at C- terminal and NcoI restriction enzyme sites on both ends using Gibson Assembly (NEB, USA). Empty vector pCAMBIA 1302 was used as a positive control ...
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bioRxiv - Plant Biology 2023Quote: ... into the pMAL-C2 vector and fusion proteins were expressed and purified using amylose-affinity chromatography as described by the manufacturer (New England Biolabs). The over-expressed recombinant proteins were then coupled to CNBr-activated Sepharose (Sigma ...
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bioRxiv - Synthetic Biology 2023Quote: ... was then done with the homologies to construct the initial green fluorescent protein (GFP) plasmid and then transformed into competent E.coli (NEB#C2984H) and subsequently sequenced (Plasmidsaurus ...
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bioRxiv - Systems Biology 2023Quote: ... The glutathione or amylose agarose was then washed 5-10 times to remove unbound proteins before boiling and analysis by SDS-PAGE WB using anti-MBP (NEB) and anti-GST (abcam ...
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bioRxiv - Plant Biology 2023Quote: ... A cDNA clone for the 2b protein of Ho-CMV was recapitulated by site-directed mutagenesis of the coding sequence of the Fny-CMV 2b protein using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). The resultant DNA product encoding the recapitulated Ho-CMV 2b protein contained the amino acid substitutions S47A ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was eluted at 100% Buffer F and protein fractions were pooled and combined with TEV and CIP (#M0525) from NEB and dialyzed overnight against Buffer E at 4 °C ...