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Citations for New England Biolabs :
1051 - 1100 of 10000+ citations for Human Carboxyl Terminal PDZ Ligand Of Neuronal Nitric Oxide Synthase Protein NOS1AP ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Purified GST-Bbs5 protein (400 μg) was incubated with 6 μl of PKC (New England Biolabs) and 10 μl of ATP (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... GST-fusion proteins on glutathione beads were pre-treated with 250 units CK2 (New England Biolabs) in protein kinase buffer (New England Biolabs ...
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bioRxiv - Developmental Biology 2021Quote: ... and the protease enzyme was further removed from hTNPO1 protein by Ni-NTA Magnetic Beads (NEB) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Precipitated proteins were wash once in acetone and resuspended in 1X blue loading buffer (NEB, B7703S).
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bioRxiv - Molecular Biology 2021Quote: ... The resulting PCR product was ligated into the pMal-c2x protein expression plasmid (New England Biolabs) adjacent to the malE gene to generate the pMAL-c2x-malE-L375-his10 plasmid encoding maltose binding protein (MBP ...
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bioRxiv - Developmental Biology 2021Quote: ... The guide RNA was injected into one-cell stage embryos together with Cas9 protein (N.E. Biolabs). Mutant animals were identified by PCR using the following primers ...
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bioRxiv - Biochemistry 2019Quote: Assays were conducted by incubating 0.1 and 1 μM SidJ in 1X Protein Kinase buffer (NEB), with 10 mM CaCl2 ...
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bioRxiv - Bioengineering 2021Quote: ... Amplified protein and pCTCON backbone were digested with BamHI and NheI restriction enzymes (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5 μM protein was incubated in the presence or absence of 20 μM SAM (NEB #B9003) in 25 mM HEPES pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... Surface biotinylated proteins immobilized on streptavidin agarose beads were denatured in Denaturing Buffer (New England Biolabs) at 100°C for 10 min in accordance with the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... All proteins were overexpressed in Escherichia coli BL21 Codon-Plus competent cells (C2527, New England Biolabs) and grown at 37 °C in 2 % Luria Broth containing 30 mg/L kanamycin until OD600 reached 0.6 ...
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bioRxiv - Microbiology 2021Quote: One microgram of full-length S protein was treated with 1 μg of FXa (P8010L, NEB), furin (P8077S ...
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bioRxiv - Cell Biology 2021Quote: ... and 1 mM MnCl2 only or together with 1 μl Lambda Protein Phosphatase (New England Biolabs). After incubation at 30°C for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... MBP-tagged protein was captured by gravity flow affinity chromatography using amylose resin (New England Biolabs). Captured protein was washed with wash buffer (50mM Tris/HCl pH 7.6 ...
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bioRxiv - Biochemistry 2022Quote: ... The fusion protein was purified from the supernatant using amylose resin (New England Biolabs, Ipswich, MA), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... together with the Cas9 protein (EnGen® Cas9 NLS, S. pyogenes, M0646M, NEB, Ipswich, MA, USA) was injected into zebrafish embryos at one-cell stage ...
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bioRxiv - Molecular Biology 2020Quote: ... half of the samples were treated with a broad-spectrum lambda protein phosphatase (NEB Cat#P0753L) and PNGase-F (Promega Cat#V4831 ...
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bioRxiv - Microbiology 2019Quote: Cellular extracts were incubated with 2,000 units of λ-protein phosphatase (λ-Ppase) (New England Biolabs) in 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein-RNA complexes were radiolabeled on-bead in 40 µl reactions with T4 polynucleotide kinase (NEB) and 2 µl [γ-32P]ATP (6,000 Ci/mmol ...
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bioRxiv - Genomics 2019Quote: Cas9/RNP complexes were assembled with 25 pmol Cas9 protein (EnGen Cas9 NLS, New England Biolabs) and 50 pmol gRNA just prior to electroporation ...
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bioRxiv - Cell Biology 2019Quote: ... SNAP-FOR1(3P,FH2) protein was labeled with SNAP-549 dye (New England Biolabs, Ipswich, MA) as per manufacturer’s instructions prior to each TIRF experiment.
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bioRxiv - Cell Biology 2020Quote: Total protein extracts were obtained from cell pellets directly resuspended in the loading buffer (Biolabs B7709S) containing 1% SDS ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were prediluted to a concentration of 750 nM in 1x CutSmart buffer (New England Biolabs) and preloaded with guide DNA or guide RNA in a 1:1 ratio at 37°C for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... All proteins were overexpressed in Escherichia coli BL21 Codon-Plus competent cells (C2527, New England Biolabs) and grown at 37 °C in 2 % Luria Broth containing 30 mg/L kanamycin until OD600 reached 0.6 ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were injected with 200-300 pg gRNAs and 1.6 ng EnGen Cas9 protein (#M0646M NEB). The target and gRNA sequences ...
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bioRxiv - Molecular Biology 2021Quote: ... The antibodies were captured using 50 µl of protein G magnetic beads (S1430, New England Biolabs) incubated for 60 min with end over end mixing at 4°C ...
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bioRxiv - Microbiology 2022Quote: All recombinant proteins were expressed in Escherichia coli T7 Express lysY/Iq cells (New England Biolabs). Except for GST-PP1γ ...
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bioRxiv - Biochemistry 2022Quote: ... CBP (Chitin Binding Protein, produced by N. Martín in Dr. Casanova’s lab, New England Biolabs Protocol) was used as a secondary antibody at 1:300 to detect chitin and visualize the tracheal branches ...
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bioRxiv - Microbiology 2022Quote: ... individual sgRNAs and Cas9 protein in 1X NEB 3.1 buffer (New England Biolabs, Ipswich, MA, USA) were incubated at 37°C for 2 h ...
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bioRxiv - Microbiology 2022Quote: ... The DST was removed by incubating the protein with 64 units of Enterokinase light chain (BioLabs) in 10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: Genetically modified zebrafish lines were created using Cas9 protein (EnGen® Spy Cas9 NLS, #M0646T, NEB) and previously established protocols combining PCR and reverse transcription to generate guide RNAs44 ...
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bioRxiv - Microbiology 2022Quote: ... The EcRecA protein was purified in a similar manner and then compared to commercial EcRecA (NEB). The protein activity of commercial NEB and purified proteins was equivalent ...
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bioRxiv - Biochemistry 2024Quote: ... crude extracts were treated with the λ-protein phosphatase (λ-PPase; New England BioLabs, Cat# P0753S) before application on the gel ...
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bioRxiv - Molecular Biology 2024Quote: The glycosylation status of the proteins was studied using the PNGase-F and the EndoH (NEB) glycosidases according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The HA protein tag and point mutations were introduced using the KLD Enzyme mix (NEB M0554S) and were verified by Sanger sequencing and whole-plasmid sequencing (Plasmidsaurus).
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bioRxiv - Cell Biology 2023Quote: ... In some cases immunoprecipitated proteins were treated with PNGase-F or Endo-H (New England Biolabs) according to the manufacturers protocols ...
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bioRxiv - Biochemistry 2023Quote: ... per 1 µg protein for 45 min at 30°C in PMP buffer (New England Biolabs) plus 1 mM MnCl2 ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate-antibody mix was then incubated with Magnetic Protein A beads (New England Biolabs, S1425S) overnight at 4 °C with end over rotation ...
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bioRxiv - Molecular Biology 2023Quote: ... Preclearing step: ∼ 50 μL magnetic beads (Protein A or G Magnetic Beads; #S1425S or #S1430S NEB) were added to the sample and incubation was carried out for 1 h on a rocking platform at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by simultaneous overnight tag cleavage by TEV and dephosphorylation by λ-protein phosphatase (NEB, P0735). The cleaved ...
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bioRxiv - Biophysics 2023Quote: ... The labelled proteins were subjected to overnight cleavage by exogenous furin (New England Biolabs, Ipswich, MA) at 37°C to fully convert GP0 to GP1 and GP2 ...
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bioRxiv - Bioengineering 2024Quote: ... In vitro cleavage reactions were performed on the circularized DNA using SpCas9 protein (NEB, Ipswich, MA) and KLRC1 sgRNA ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples collected from culture media were treated with PNGase F enzyme (New England Biolabs, P0709S) to remove the N-linked oligosaccharides from the TECTA protein according to the manufacturer protocol ...
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bioRxiv - Cell Biology 2024Quote: ... AcGFP and TagRFP-657 proteins and SNAP-Surface 488 and Alexa 647 dyes (New England Biolabs) were measured in the same excitation and emission ranges as in flow cytometry assays ...
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bioRxiv - Neuroscience 2024Quote: ... and used to form ribonucleoprotein (RNP) complexes when combined with recombinant Cas9 protein (New England Biolabs). Ribonucleoprotein complexes were formed using the method of Kouranova49 ...
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bioRxiv - Biochemistry 2024Quote: ... reactions were performed with 6 μl of PURExpress in vitro protein synthesis system (New England Biolabs). The reactions were carried out on different templates (Supplementary Table 2) ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were eluted with lysis buffer + 250 mM imidazole and bound to chitin-resin (S6651L, NEB), before washing in lysis buffer and overnight incubation with lysis buffer + 200 mM DTT and his-ULP1 protease ...
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bioRxiv - Bioengineering 2024Quote: Protein was expressed for in vitro assays in T7 Express cells (New England Biolabs, Catalog # C2566H) in 96-well deep-well plates using the pET22b(+ ...
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bioRxiv - Microbiology 2023Quote: ... using the kit Luna® Universal qPCR Master Mix Kit (New England Biolabs, Ipswich, MA, United States). PCRs were conducted in a total volume of 20 µl containing 10 μL of Luna Universal qPCR Mix ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were then purified using the DNA cleanup column kit (the Monarch PCR & DNA cleanup kit from NEB).