Labshake search
Citations for New England Biolabs :
1051 - 1100 of 3706 citations for Alpha 1 6 Fucosyltransferase FUT8 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: ... These SETD3 mutants were introduced into either EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) or EcoRV-digested pLenti-CMVTRE3G-Puro-DEST (w811-1) by Gibson Assembly (New England Biolabs).
-
bioRxiv - Biophysics 2022Quote: ... These SETD3 mutants were introduced to either EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) or EcoRV-digested pLenti-CMVTRE3G-Puro-DEST (w811-1) by Gibson Assembly (New England Biolabs).
-
bioRxiv - Biophysics 2022Quote: ... These SETD3 mutants were introduced to either EcoRV-digested pLenti-CMV-Puro-Dest (w118-1) or EcoRV-digested pLenti-CMVTRE3G-Puro-DEST (w811-1) by Gibson Assembly (New England Biolabs).
-
bioRxiv - Plant Biology 2021Quote: ... RNA adapters with a 5’ inverted dT modification (See Supplementary Table 1) were ligated to the DNase- treated RNA by T4 RNA ligase 1 (#M0204S, New England Biolabs) to render the canonical cleavage products not available for subsequent ligation reaction ...
-
bioRxiv - Molecular Biology 2020Quote: ... Samples were ligated to barcode primers 1-20 of NEBNext Index Primers for Illumina sets 1 and 2 (New England Biolabs) and libraries analysed using DNA High Sensitivity chip on an Agilent 2100 Bioanalyzer before being pooled ...
-
bioRxiv - Molecular Biology 2020Quote: ... 10 mM sodium ascorbate before collecting them by scraping in 1 mL of the same solution with Murine RNase Inhibitor (1:1000, NEB). Cells were collected in a microcentrifuge tube ...
-
bioRxiv - Genomics 2021Quote: ... End fill-in and A-tailing were performed by addition of Klenow Fragment 3’ --> 5’ exo-(Enzymatics) and dNTP mix (10 mM dATP, 1 mM dCTP, 1 mM dGTP New England Biolabs). After ligation to methylated Illumina TruSeq LT v2 adaptors using T4 DNA Ligase rapid (Enzymatics) ...
-
bioRxiv - Biochemistry 2021Quote: ... were 3′ end-radiolabeled by incubation for 1 hr at 16°C with 1.11 MBq [5′-32P] cytidine-3′,5-bisphosphate (222 TBq mmol−1, Hartmann Analytic) and 10 units T4 RNA ligase 1 (NEB) in a total reaction volume of 20 µl containing 15% (v/v ...
-
bioRxiv - Cell Biology 2020Quote: 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1x NEB 1 buffer, 1% triton X-100, and 100 U HpaII enzyme (NEB)) and incubated at 37°C for 3 hours with constant shaking ...
-
bioRxiv - Biochemistry 2022Quote: BsmBI-digested vector and insert were ligated in a molar ratio of 1:100 to a total of 1 μg using T4 DNA ligase (NEB) for 2 h at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... was added reconstituted with 1 μg of purified recombinant αS (vendor) in 1 μL of 5X RNA Polymerase Reaction Buffer (New England Biolabs) and incubated at 4°C for 15 minutes ...
-
bioRxiv - Molecular Biology 2023Quote: ... ligation reactions with T4 DNA ligase were prepared: 1 μM of ssPCRP was added to 1 μM complementary strands in 1X T4 ligation buffer (NEB) in a final volume of 10 μL ...
-
bioRxiv - Neuroscience 2022Quote: 4 µl procainamide-labeled glycans were combined with 1 µl 10x sodium acetate – Ca2+ buffer (Glycobuffer 1, New England Biolabs), 1 µl 10x BSA (diluted 1:10 with Milli-Q water from a 100X stock ...
-
bioRxiv - Molecular Biology 2024Quote: ... adding nuclease-free water to a final volume of 1 L) with 1:1000 (vol/vol) proteinase K (New England Biolabs) at 37°C for 1 h ...
-
The RNA-binding protein RbpB is a central regulator of polysaccharide utilization in gut BacteroidesbioRxiv - Microbiology 2023Quote: ... 20 pmol of the dephosporylated RNA were 5’-end-labeled (20 µCi of 32P-γATP) in a 20 µL reaction for 1 h at 37°C using 1 U polynucleotide kinase (NEB). Labeled RNA was purified on a G50-column (GE Healthcare ...
-
bioRxiv - Biophysics 2024Quote: ... Splint ligation reaction was done by mixing DNA in equal molar ratios of 1 mM in a 20 μl reaction and annealed in 1✕ ligation buffer (NEB) over 4 hours using a thermocycler (bio-rad) ...
-
bioRxiv - Bioengineering 2024Quote: ... containing 0.06 % pluronic acid (F-127, final concentration) and 1 µL of each disulfide bond enhancer 1 and 2 (NEB #E6820). We used droplet oil consisting of 3M™ Novec™ HFE7500 Engineered Fluid (3M ...
-
bioRxiv - Microbiology 2023Quote: Verified amplicons were combined with the pMV306H integrative vector backbone at a ratio of 1:1 v/v and allowed to ligate overnight at 4°C with T4 DNA ligase (NEB). Ligation products were subsequently transformed into chemically competent DH5ɑ E.coli ...
-
bioRxiv - Synthetic Biology 2023Quote: Gel electrophoresis was performed using 1 % agarose gels and the ladders used were either Quick-Load 1 kb Extend DNA Ladder (NEB), or Quick-Load Purple 1 kb plus DNA Ladder (NEB).
-
bioRxiv - Developmental Biology 2023Quote: ... 1 μg of cDNA was amplified with Phusion polymerase for 32 cycles and separated on a standard 1% agarose gel together with a 100 bp or 1 kb ladder (New England Biolabs). Ribosomal protein L32 (RpL32 ...
-
bioRxiv - Molecular Biology 2023Quote: ... DNA was PCR amplified (primers hFUR Exon 1-3 PCR For and hFUR Exon 1-3 PCR Rev) and purified (Monarch PCR & DNA cleanup kit, NEB). The resulting PCR product was sequenced using the hFur Exon 1 Seq primer.
-
bioRxiv - Genomics 2023Quote: ... beads were incubated with 10 µL of USER mix (1 µL of 10X USER buffer and 1 µL of USER enzyme in 8 µL of nuclease-free water, NEB) and incubated at 37°C for 15 minutes ...
-
bioRxiv - Biochemistry 2022Quote: ... 50 μg of supercoiled pUBER plasmid was treated with 1 unit/μg Nt.BbvCI in 1 × Cutsmart buffer (New England Biolabs, USA) at 37°C for 4 h ...
-
bioRxiv - Biochemistry 2023Quote: ... were ligated to 50 pmol RNA oligonucleotide “20.25” (5’-UCG AAG UAU UCC GCG UAC GU-3’, Dharmacon) with 1 µL T4 RNA ligase 1 (NEB, #M0204S) for 1 h at 16 °C in 15% (v/v ...
-
bioRxiv - Biophysics 2023Quote: ... and a reverse elongation primer (Supplementary Tables 1 and 2) and incubating for 1 cycle of annealing/extension with Q5 polymerase (New England Biolabs). dsDNA product was then incubated with ExoSAP-IT (Applied Biosystem ...
-
bioRxiv - Cancer Biology 2023Quote: Fresh omentum and omental HGSOC tumor metastasis biopsy samples were cut into small pieces and dissociated in digestion solution (1 mg/mL collagenase/Dispase [Sigma cat. no. 10269638001], 1 unit/mL DNase I [NEB, cat ...
-
bioRxiv - Microbiology 2023Quote: ... A 1 ml aliquot of cells was incubated with 1 μl M13KO7 (1011 plaque-forming units per ml, pfu/ml) (NEB) for 10 min on ice to allow attachment ...
-
bioRxiv - Neuroscience 2024Quote: ... The cut organoids were placed in pre-warmed Accutase supplemented with 1:2000 DNAseI and 1:2000 RNAase Inhibitor (both NEB), and slowly pipetted up and down using wide-bore pipette tips ...
-
bioRxiv - Microbiology 2024Quote: Lysates were treated with 20 μg·ml-1 of RNase A and 2U·ml-1 of DNase I (New England Biolabs, Whitby, Canada) along with 10% DNase buffer 10x at 37°C for 30 min followed by heat inactivation at 75°C for 10 min ...
-
bioRxiv - Genetics 2024Quote: ... Each assay was prepared to contain a final concentration of 1× PrimeTime Master Mix (IDT) or 1× Luna Universal qPCR Master Mix (NEB), 0.1 μM for each of the two probes (LNA9K1-Gly ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 µM of sgRNA scaffold and a RPA1-specific forward primer and were mixed in 1× HiFi reaction buffer with MgCl2 and 1 unit Phusion High-Fidelity DNA Polymerase (New England BioLabs), 25 µl total volume ...
-
bioRxiv - Neuroscience 2024Quote: ... pH 8.0) for 1 h at 37 °C and washes in CSTX buffer (1× CutSmart buffer (New England Biolabs (NEB) B60004) ...
-
bioRxiv - Microbiology 2024Quote: ... A 20 µL Gibson Assembly reaction was performed with 50 ng of vector at a 2:1 ratio for 1 hour (New England Biolabs NEBuilder HiFi DNA Assembly Master Mix ...
-
bioRxiv - Microbiology 2024Quote: ... incubation at 30 °C for 1 h) and removed together with unused Linker 1 in a 30 min incubation with exonuclease RecJ (NEB). The reaction was stopped by heating at 65 °C for 20 min and products purified using a DyEx spin column (Qiagen) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... We incubated 1 µg of DNA origami nanoparticle with 1 U/µL DNase I (2,000 units/mL, New England Biolabs, M0303S) with 10× DNase I buffer diluted in water (Gibco) ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 1 µL of murine RNase inhibitor (NEB) in a 50 μL final reaction volume ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 µl RNase H (5U final, NEB M0297L) was added directly to each reaction and incubation for 20 min at 37°C ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and 0.5U μl-1 I-Scel enzyme (NEB) in water were incubated at 37°C for 30 minutes and then micro-injected at a volume of approximately 2nl per embryo into lamprey embryos at the one-cell stage ...
-
bioRxiv - Developmental Biology 2022Quote: ... and 1 μL of PNK enzyme (NEB M0247S). During the incubation ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 1 µl of BsaI-HFV2 (New England Biolabs), up to 20 µl of nuclease-free H2O ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 μL MgSO4 (4 mM; Biolabs, Ipswich, MA), 1.4 μL dNTPs (1.4 mM ...
-
bioRxiv - Neuroscience 2020Quote: ... 1:1000 concentrations of SNAP-Surface 488 (NEB #S9124 ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 333 units RNA Ligase 1 (NEB M0204S) with incubation at 16°C for ~16-18 hours ...
-
bioRxiv - Molecular Biology 2021Quote: ... treated with apyrase (25 mU mL−1, NEB) and incubated at room temperature for another one hour ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 μl BsmBI (10,000 units/ml, NEB, R0580), and water was added to 14 μl ...
-
bioRxiv - Cell Biology 2020Quote: ... and 1 μL ENGEN Spy Cas9 NLS (NEB) and allowing the mix to incubate at room temperature for at least 10 min ...
-
bioRxiv - Genomics 2020Quote: ... 0.5 µl T4 RNA ligase 1 (5U; NEB)] was added and reactions were incubated at 25°C for 1 hour ...
-
bioRxiv - Genomics 2020Quote: ... 1 µl 10x T4 RNA ligase buffer (NEB), 0.25 µl SUPERase-In ...
-
bioRxiv - Biochemistry 2021Quote: ... 1 µL T4 DNA Ligase Buffer (NEB; B0202A), 1 µL of the insert and plasmid DNA ...
-
bioRxiv - Biochemistry 2021Quote: ... and 10 U T4 RNA ligase 1 (NEB) and 1 μM of 5’ adaptor oligo (total reaction volume = 20 μl) ...