Labshake search
Citations for New England Biolabs :
1051 - 1100 of 2049 citations for 6 Benzyloxy 1H indole 2 boronic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... Secondary amplification reactions were performed using NEBNext high-fidelity 2 PCR master mix (NEB) in 50 μl reactions (26 μl of 2X mix ...
-
bioRxiv - Molecular Biology 2024Quote: ... 20 µL of reactions were treated with 2 µL DNase I (NEB, Cat # M0303S) in a total volume of 100 µL at 37°C for ∼ 20 min before further analysis and treatment.
-
bioRxiv - Immunology 2023Quote: ... and 2 µL of home-made Gibson mix (T5 exonuclease (0.2U; New England Biolabs), Phusion polymerase (12.5U ...
-
bioRxiv - Developmental Biology 2024Quote: ... R0148S)2 or the wild-type allele was digested with HphI (New England Biolabs, R0158S) for 1 hour ...
-
bioRxiv - Genomics 2024Quote: ... and mixed with an equal volume of 2 x RNA loading dye (NEB, B0363S). 25 μl of the mixture was loaded on a 10% TBE-Urea gel (Bio-Rad ...
-
bioRxiv - Cell Biology 2024Quote: ... all kinases were tested for activity using 2 µg each of histone H1 (NEB) or Myelin Basic Protein (MBP ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA was ligated with 3′-adenylated adapters using T4 RNA Ligase 2 (NEB #MO351) for 4 hrs at 16°C ...
-
bioRxiv - Microbiology 2023Quote: ... The annealed constructs were ligated using 2 µL T4 RNA ligase2 (NEB, 10U/µL), 3 µL 0.1% BSA (Ambion) ...
-
bioRxiv - Molecular Biology 2023Quote: ... two pairs of oligonucleotides (Suppl. Table 2) were phosphorylated and ligated into BsbI (NEB) digested pDG459 plasmid ...
-
bioRxiv - Molecular Biology 2023Quote: ... a pair of oligonucleotides (Suppl. Table 2) was phosphorylated and ligated into BsbI (NEB) digested pX459 and pX458 plasmids ...
-
bioRxiv - Genomics 2023Quote: ... 20 of 2 U µL-1 Phusion High-Fidelity DNA Polymerase (New England Biolabs), 160 µL of 40 U µL-1 Taq DNA ligase (New England Biolabs ...
-
bioRxiv - Genomics 2023Quote: ... Forked adapters (Supplementary Table S7) were annealed in NEBuffer 2 1X (NEB cat. B7002S) to a final concentration of 20 µM in a thermal cycler set to heat at 98°C and gradually cool down to 4°C with a 1°C per 20 sec gradient.
-
bioRxiv - Biochemistry 2022Quote: ... 2 and cloned into pET28a using T4 DNA ligase (New England Biolabs GmbH, Germany). The gene encoding ribose-phosphate pyrophosphokinase (RPPK ...
-
bioRxiv - Bioengineering 2022Quote: ... Samples requiring dephosphorylation were treated with 2 U/μL λPP (New England Biolabs, # P0753) for 3 h at 30°C and then washed with PBSCM ...
-
bioRxiv - Cancer Biology 2022Quote: ... DNase I (2 µL, 2,000 U/ml, RNase-free, New England Biolabs, Ipswich, MA), 10× DNase buffer ...
-
bioRxiv - Molecular Biology 2023Quote: ... and inserted into the pGLuc Mini-TK 2 Gaussia luciferase enhancer reporter plasmid (NEB) via KpnI/SacI restriction sites ...
-
bioRxiv - Bioengineering 2022Quote: ... 10 μL PCR reactions were set-up using 2 μL 5x HF Buffer (NEB), 0.5 μL 10 mM dNTPs (NEB) ...
-
bioRxiv - Biophysics 2023Quote: ... 2 pmol were digested to single nucleosides using the Nucleoside Digestion Mix (NEB # M0649), and the ratio of Ψ s versus uridines was determined via tandem quadrupole mass spectrometry (Supplementary Figure S2a).
-
bioRxiv - Microbiology 2023Quote: ... 20-μL reactions contained 2 μM enzyme and 250 μM NTPs (New England BioLabs) in reaction buffer with 50 mM Tris-HCl (pH 7.5) ...
-
bioRxiv - Developmental Biology 2023Quote: ... On-Bead 5’ Decapping reaction was done using NEBuffer 2 buffer (NEB, B7002S ®). Off-Bead Reverse Transcription followed the instructions in SuperScript ® IV Reverse transcriptase kit ...
-
bioRxiv - Genetics 2024Quote: ... the DNA-bound beads were digested with 2 μl BpmI (NEB, catalog number R0565L) for 30 min at 37 °C ...
-
bioRxiv - Developmental Biology 2024Quote: ... Libraries were generated via PCR amplification using NEBNext HighFidelity 2× PCR Master Mix (NEB). The resultant libraries were purified again using the MinElute PCR Purification Kit (Qiagen) ...
-
bioRxiv - Genomics 2024Quote: ... 0.4 µL RNase Inhibitor and 2 µL Induro RT (200 U/µL; NEB M0681S) were incubated for 15 minutes at 60°C ...
-
bioRxiv - Molecular Biology 2024Quote: ... in 100 μl final volume per coverslip using: 2 μl T4 Ligase (NEB; #M0202S), 5 μl 10 μM biotinylated linker (5′-TACTACCTCGAGAGTTACGCTAGGGA-TAACAGGGTAATATAGTTT[biotin– dT]TTTCTATATTACCCTGTTA-TCCCTAGCGTAACTCTCGAGGTAGTA-3′) ...
-
bioRxiv - Molecular Biology 2024Quote: ... The digested nuclei were ligated with 2 μl of T4 DNA ligase (NEB M0202S) in a total ligation volume of 2 ml at 16°C for 12 h.
-
bioRxiv - Developmental Biology 2024Quote: ... Sequencing libraries were prepared with NEBNext High-Fidelity 2×PCR Master Mix (NEB, M0541S).
-
bioRxiv - Biochemistry 2024Quote: ... 1 µL of Buffer 2 and 0.5 µL of rSAP (New England Biolabs, M0371S) pre-mixed with 0.5 µL of 50% glycerol were added ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 2 µL of the annealed product were mixed with 0.5 U Klenow Fragment (NEB), 33 µM dNTPs ...
-
bioRxiv - Genomics 2024Quote: ... DNase I (2 µL, 2,000 U/ml, RNase-free, New England Biolabs, Ipswich, MA), 10× DNase buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 μM of dsDNA was incubated with CpG methyltransferase (M. SssI, New England Biolabs) in the presence of 200 μM of S-adenosylmethionine ...
-
bioRxiv - Immunology 2024Quote: ... washed in 10 mL RPMI+10% FBS + 2 µl RNase-free DNase I (NEB), and placed in single-cell suspension in 1 mL RPMI+10% FBS ...
-
bioRxiv - Immunology 2024Quote: ... washed in 10 mL RPMI+10% FBS + 2 µl RNase-free DNase I (NEB), and stained with LIVE/DEAD Aqua for 15 minutes at room temperature ...
-
bioRxiv - Biophysics 2024Quote: ... Vector DNA was prepared by digestion with EcoRI (NEB, R3101S, 2 unit/μg DNA) and HindIII (NEB ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Cells were recovered in 2 mL of 37°C SOC recovery medium (NEB #B9020S) at 37°C for 1 hour ...
-
bioRxiv - Bioengineering 2024Quote: ... USER enzyme digestion: 500 ng aliquots were digested with 2 µL USER Enzyme (NEB) at 37°C for 3 hours ...
-
bioRxiv - Cancer Biology 2024Quote: ... and 2 µl of T4 DNA ligase (2,000,000 U/ml, NEB, cat. no. M2200M), for 15 min at RT with 3 µl of the USER adaptor (NEBNext Multiplex Oligos for Illumina ...
-
bioRxiv - Genetics 2021Quote: ... 0.5 μM MPRA_v3_F and MPRA_v3_20I_R primers (Supplementary Table 14) and 2 ng BSA (NEB, B9000). PCR master mix was emulsified by vortexing with 220 μL Tegosoft DEC (Evonik) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... treated ~2 μg RNA with RNase-free DNase I (Catalog# M0303S NEW ENGLAND Biolabs, USA), then used 1 μL treated RNA in cDNA synthesis with SuperScript III Reverse Transciptase (Invitrogen ...
-
bioRxiv - Molecular Biology 2022Quote: pLXV-EF1alpha-2xStrep-SARS-CoV-2-nsp14-IRES-Puro was opened with BsrGI-HF (NEB) and EcoRI-HF (NEB) ...
-
bioRxiv - Developmental Biology 2022Quote: ... samples were washed in PBS-T and blocked in PBS-T + 2% BSA (NEB, B9000) + 5% donkey/goat serum (Jackson Immunoresearch/Dianova ...
-
bioRxiv - Molecular Biology 2020Quote: ... or 500 ng of chromatin-bound or nucleoplasmic RNA with 2 µl Quick CIP (NEB) in a total volume of 20 µl for 90 min at 37°C ...
-
bioRxiv - Genomics 2020Quote: ... add 2 μl 10 mM dATP and 5 μl Klenow Fragment (3’ −> 5’ exo-) (NEB) to digest the unligated DNA fragments at 37°C for 40 min ...
-
bioRxiv - Biochemistry 2020Quote: ... pre-tRNA stock was diluted 1 in 2 into RNA loading buffer (New England Biolabs) and separated on a 10% acrylamide urea-TBE denaturing gel ...
-
bioRxiv - Immunology 2021Quote: ... PCRs were performed with Phusion High-Fidelity DNA Polymerase (2 U/µL) (New England Biolabs) in a total volume of 50 μl ...
-
bioRxiv - Genomics 2020Quote: ... 5 μL of Phusion High Fidelity 2× Master mix (New England Biolabs, Beverly MA, USA) and 2 μL of 10 μM standard Illumina P1 and P2 primers ...
-
bioRxiv - Bioengineering 2020Quote: ... PCR was performed using Q5® High-Fidelity 2× Master Mix (New England Biolabs, US). The point mutation in dCas9 to create H840A Cas9n was made using Q5® Site-Directed Mutagenesis Kit (New England Biolabs ...
-
bioRxiv - Bioengineering 2020Quote: ... or colony PCR using Q5® High-Fidelity 2× Master Mix (New England Biolabs, US) if a chromosomal region was targeted ...
-
bioRxiv - Biochemistry 2021Quote: ... The sample was then resuspended in 100 μl/sample volume of 1X NEBuffer 2 (NEB) supplemented with Triton X-100 to 0.1% and transferred to a new tube ...
-
bioRxiv - Cancer Biology 2021Quote: ... were incubated at 37 °C for 2 hours in 1x T4 DNA ligase buffer (NEB). Following this incubation 1200 units T4 DNA ligase (NEB ...
-
bioRxiv - Microbiology 2021Quote: ... Genomic DNA (2 μg) was digested with the AluI restriction enzyme (New England Biolabs, U.S.A) and purified by using an illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare ...