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Citations for New England Biolabs :
1001 - 1050 of 1187 citations for Yellow Fever virus Envelope protein sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: The sf-GFP reporter protein was expressed essentially as described 38 in the PURExpress ΔRF123 system (New England Biolabs). The DNA template was PCR-amplified from the pY71sfGFP plasmid 58 by PCR using primers sfGFP-fwd and sfGFP-rev (all primers are listed in Supplementary Table S2) ...
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bioRxiv - Biochemistry 2023Quote: Trx-linker concatemers (1 mg/mL) were incubated with 50,000 units of the catalytic subunit of cAMP-dependent protein kinase (PKA) (NEB)—which recognizes the RRAS motif within the central linker of the Trx-linker nonamer—in protein kinase buffer (50 mM Tris HCl ...
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bioRxiv - Biophysics 2023Quote: ... and DNA-bound proteins were released by MNase treatment (2 min 30° with 700 units of MNase NEB # M0247S) and analyzed by gel electrophoresis40.
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bioRxiv - Cell Biology 2023Quote: ... Total lysates (200 µg each) were incubated with or without 400 U lambda protein phosphatase (New England Biolabs, #P0753S) and 1 mM MnCl2 at 30 °C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: Purified and concentrated mTAAR7f was mixed with about 10x molar excess of both the heterotrimeric G protein and Nb35 and 0.5 U apyrase (NEB) and incubated overnight ...
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bioRxiv - Biochemistry 2023Quote: Approximately 100 µg of extracted protein was incubated with 2 µL (1,000 U) of PNGase F (New England Biolabs) at 37°C for 48 hrs ...
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bioRxiv - Cell Biology 2023Quote: For protein synthesis the verified plasmid was transformed into BL21 competent cells (C2530, New England BioLabs, Ipswich, MA, USA). Cells were grown shaking at 37°C in 2XYT with appropriate selection to an OD600 of 0.6 and then transferred to 18°C ...
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bioRxiv - Immunology 2023Quote: ... Glycan removal was performed on 20 µg or 25 µg total protein using PNGase F (#P0704; New England Biolabs), Endo H (#P0702 ...
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bioRxiv - Bioengineering 2023Quote: ... 20 ng of each integrase amplicon was used per reaction using PURExpress® In Vitro Protein Synthesis Kit (NEB) in a total volume of 9 µl ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing CrtSPARTA protein complex was pooled and further loaded onto an amylose resin column (New England Biolabs). The column was washed with 20 column volumes (CV ...
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bioRxiv - Biophysics 2023Quote: ... We then sequentially labeled Nup96 proteins with SNAP ligand conjugated Alexa Fluor 647 (BG-AF647, S9136S, New England Biolabs) and mitochondria with primary anti-Tomm20 antibodies (rabbit polyclonal ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 20 µL of denatured protein lysate was combined with 2.5 µL of 10X GlycoBuffer 3 (Cat.#B1720S; New England Biolabs) and 2.5 µL of Endo Hf in a total reaction volume of 25 µL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 20 µL of denatured protein lysate was combined with 2.6 µL of 10X GlycoBuffer 2 (Cat.#B3704S; New England Biolabs), 2.6 µL of 10% NP-40 (Cat.#B2704S ...
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bioRxiv - Developmental Biology 2024Quote: ... A cocktail of guide RNAs for each target (1 μL each) was pre-incubated with Cas9 protein (NEB #M0646M) and 0.68uL KCl (as described in (100)) ...
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bioRxiv - Microbiology 2024Quote: ... protein aliquots were subjected to PNGase F or a broad range mannosidase (α1,2/3/6) (New England Biolabs, France) digestion according to the manufacturer’s specifications ...
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bioRxiv - Microbiology 2024Quote: ... the protein eluted from MonoQ 5/50 GL was incubated with 10 μM SNAP-Surface Alexa Fluor 647 (NEB) for 1 hour at 4°C prior to gel filtration.
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bioRxiv - Biophysics 2024Quote: M2R constructs were dephosphorylated through a 30-minute room temperature treatment with 200 units of lambda protein phosphatase (NEB), 10 units of calf intestinal phosphatase (NEB) ...
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bioRxiv - Developmental Biology 2024Quote: ... a mix containing 18µM sgRNA and 12µM Cas9 protein (EnGen™ Spy Cas9-NLS S. pyogenes, NEB Cat# M0646T) was incubated for 10 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Lipidation-deficient LC3B proteins were generated by mutagenic PCR using Q5 Hot Start High-Fidelity Master Mix (New England Biolabs) to introduce the GGG to GCT change that leads to a glycine to alanine (G120A ...
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bioRxiv - Immunology 2020Quote: ... was inserted into a published oligonucleotide scaffold (Talbot and Amacher, 2014) and injected together with recombinant Cas9 protein (New England Biolabs) into 1-2 cell stage zebrafish (AB strain) ...
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bioRxiv - Developmental Biology 2020Quote: ... We first joined sequences for fluorescent protein mCerulean (CFP) and 2A peptide P2A (a ribosomal skip sequence) with Q5 (NEB) fusion PCR and added them to the pWPXL vector with Gibson Assembly Master Mix (NEB) ...
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bioRxiv - Microbiology 2020Quote: ... glabrata total cell lysates using the anti-Rad53 antibodies described in the previous section and Protein A magnetic beads (New England Biolabs). The immunoprecipitated samples were run on 8% acrylamide gels ...
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bioRxiv - Molecular Biology 2022Quote: The coding sequence of the BTB domain of selected ZBTB family proteins was amplified from cDNA derived from the human HCT116 cell line using Q5 High-Fidelity DNA Polymerase (NEB). Specifically for the Patz1 construct ...
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bioRxiv - Evolutionary Biology 2022Quote: All constructs encoding for the protein fragments that were used as the tagless analyte in SPR experiments were produced using the IMPACT protein purification kit (NEB) following the manufacturer’s guidelines for cloning and refolding from denaturing conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 mM EDTA) at 65°C for 15 minutes and later the proteins were digested by 10U Proteinase K (NEB) at 37°C for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 μg/well of purified BSA (NEB) in a volume of 50 μl ...
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bioRxiv - Neuroscience 2019Quote: ... pRSET-dNOS exon 16 DNA was then transformed into T7 Express lysY/lq E.coli protein expression cells (New England Biolabs #C3013) and plated on LB plus ampicillin (60 µl/ml) ...
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bioRxiv - Biochemistry 2019Quote: ... Precipitated proteins were removed by centrifugation at 14000g for 30 min at 4°C and the remaining soluble protein was incubated with enterokinase light chain (NEB) for 16h at room-temperature as per manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... MBP-fusion proteins were purified with amylose resin following the manufacturer’s instructions (GE Healthcare, Pierce, Agilent Technologies and New England Biolabs). MBP-fusions (1 µg each in 100 µl of 1% NP40 ...
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bioRxiv - Cancer Biology 2019Quote: ... were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 µg/well of purified BSA (NEB) in a volume of 50 µl ...
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bioRxiv - Neuroscience 2020Quote: ... human parkin were expressed as 6His-Smt3 fusion proteins in Escherichia coli BL21 (DE3) Codon-Plus RIL competent cells (C2527, New England Biolabs) as previous described22,33,52 ...
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bioRxiv - Biochemistry 2021Quote: ... K4A with K3WA) mutants of the full-length RAD51AP1 protein were generated by Q5 Site-Directed Mutagenesis kit (New England Biolabs) following the instructions of the manufacturer and the primer pairs listed in Table 1.
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bioRxiv - Neuroscience 2021Quote: ... genomic PCR products containing the target sites of selected gRNAs were incubated with SpCas9 protein (New England Biolabs, Ipswich, MA) following the manufacturer’s protocol and analyzed on 2% agarose gel stained with ethidium bromide ...
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bioRxiv - Microbiology 2021Quote: ... The D614G S-protein was generated by introducing the mutation into the Wuhan reference strain via Q5 Site-directed mutagenesis (NEB). Other individual mutations were subsequently introduced into the D614G S by the same process ...
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bioRxiv - Neuroscience 2020Quote: ... human parkin were expressed as 6His-Smt3 fusion proteins in Escherichia coli BL21 (DE3) Codon-Plus RIL competent cells (C2527, New England Biolabs) as previous described 44-46 ...
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bioRxiv - Biochemistry 2020Quote: ... λex = 488 nm and λem = 526 nm were used for detection of covalently bound flavin and λex = 670 nm and λem = 633 nm for visualisation of the Blue Prestained Protein Standard (New England Biolabs).
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bioRxiv - Microbiology 2021Quote: ... or beads alone were suspended in 1x NEBuffer™ for Protein Kinases with 200μM ATP (Thermo) and 100 units of CK2 (NEB) at 30°C for 45 minutes ...
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bioRxiv - Microbiology 2021Quote: Protein bound glutathione Sepharose beads were resuspended in 1x NEBuffer™ for Protein Kinases supplemented with 1mM ATPγS and 10 units of CK2 (NEB) and incubated at 30°C for 45 minutes ...
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bioRxiv - Microbiology 2021Quote: The CV-B3 Δ116-2C protein used for crystallogenesis was produced in Escherichia coli T7 Iq Express pLysS (New England BioLabs). Cells were grown in TB medium containing 100 µg/mL of ampicillin and 34 µg/mL chloramphenicol at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... cholerae H-NS protein was purified as previously described (4) using the IMPACT-kit (New England Biolabs, Catalogue No. #E6901S). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μg of tissue or cell lysates were treated with or without 2 μL Lambda Protein Phosphatase (LPP) (New England Biolabs) at 30 °C for 15 min (30 min for HEK-293E lysate ...
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bioRxiv - Biochemistry 2022Quote: 30 uL of tagged Fluc-WT proteins were incubated with Factor Xa (NEW ENGLAND BioLabs, final concentration 67 ug/mL) for 2 hours or overnight on ice.
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bioRxiv - Immunology 2022Quote: ... produced by natural mating were microinjected in the cytoplasm with a mixture of 0.33 µM EnGen Cas9 protein (New England Biolabs, M0646T), Arid5a-guide2 and Arid5a-guide5 (21.23 ng/µl (∼0.66 µM ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA-protein complexes were radioactively labeled with 32P-γ-ATP (20 μCi) using 40U T4 Polynucleotide Kinase (New England Biolabs) in supplied PNK buffer for 30 min at 37ºC ...
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bioRxiv - Biophysics 2022Quote: ... The generated DNA tether was then ligated to the DNA-protein hybrid by overnight incubation with T4 ligase (New England Biolabs) at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... In vitro Cas9 cleavage assay was carried out using purified IVT product and Cas9 protein (Cas9 Nuclease, S. pyogenes, New England BioLabs) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 40 μl of filtered double-distilled water and 20 or 30 μl of Blue Protein Loading Dye (New England Biolabs) were added to the beads or the inputs ...
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bioRxiv - Neuroscience 2022Quote: ... A 7.8 kb cassette that includes 5.2 kb DDX5 promoter sequence and 2.3 kb encoding a DDX5-GFP fusion protein was cloned into pDest-Tol2 vector (Kwan et al., 2007) through Gibson assembly (#E5510, NEB). Gibson assembly primers were designed using NEBuilder Assembly Tool (http://nebuilder.neb.com/) ...
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bioRxiv - Biochemistry 2022Quote: SNAP–tagged kinesin-8B-MD proteins (20 μM) were biotinylated in 50 μl reaction volumes by incubating with 40 μM SNAP-biotin (NEB) at 4 °C overnight ...
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bioRxiv - Biochemistry 2022Quote: In vitro protein translation was carried out (in absence of the release factors) using the PURExpress ΔRF123 kit (New England Biolabs). This guaranteed the stabilization of the ternary complexes mRNA-affitin-Ribosome ...