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Citations for New England Biolabs :
1001 - 1050 of 10000+ citations for Rat Heat Shock Protein Beta 8 HSPB8 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Eluted proteins were incubated with TEV protease and MBP-fusion endoglycosidase (EndoHf, New England Biolabs) at 4 °C overnight ...
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bioRxiv - Genomics 2021Quote: - T4 gene 32 protein (10 mg/mL, NEB or EPFL “homemade” T4g32p, 10 mg/mL). In 96-well plates ...
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bioRxiv - Biochemistry 2020Quote: Tsetse salivary proteins were treated with peptide-N-glycosidase F (PNGase F, New England Biolabs), which cleaves all N-linked glycans except those with an α-1,3 fucose modification of the chitobiose core [20] ...
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bioRxiv - Bioengineering 2020Quote: ... we use Western Blot to detect spike protein with/without treatment of PNGase F (NEB). 100 ng particles were incubated with Glycoprotein Denaturing Buffer at 98°C for 10 min ...
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bioRxiv - Plant Biology 2021Quote: ... 300 ng of gRNA transcript was bound to a purified Cas9 protein (New England Biolabs) for 10 minutes at 25° C ...
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bioRxiv - Biochemistry 2021Quote: ... or Endoglycosidase H (translation products of ∼50-150 kDa protein substrates; New England Biolabs, P0702S). All samples were solubilised for 12 h at 37°C and then sonicated prior to resolution by SDS-PAGE (10% or 16% PAGE ...
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bioRxiv - Biochemistry 2021Quote: ... Initial protein-level deglycosylation was performed using 250 U of Endo H (New England Biolabs) for up to 5 μg trimer ...
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bioRxiv - Plant Biology 2020Quote: ... MBP and GST fusion proteins were separated from supernatants using amylose resin (New England Biolabs) and Glutathione Sepharose 4 Fast Flow (GE Healthcare Life Sciences) ...
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bioRxiv - Immunology 2020Quote: The I53-50A and I53-50B.4.PT1 proteins were expressed in Lemo21(DE3) (NEB) in LB (10 g Tryptone ...
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bioRxiv - Microbiology 2021Quote: Recombinant proteins were expressed in Escherichia coli T7 Express lysY/Iq cells (New England Biolabs). For all proteins except PP1γ ...
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bioRxiv - Biophysics 2020Quote: ... The overhang DNA was added to the protein-oligo chimera together with T4 ligase (NEB) and incubated for 30 min at 16°C followed by 30 min on ice ...
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bioRxiv - Cell Biology 2021Quote: ... For embryo microinjection the annealed sgRNA were complexed with EnGen Cas9 protein (New England Biolabs) at room temperature for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... C57BL/6NJ zygotes were co-injected with Cas9 protein (50 ng/μl) (New England Biolabs) or Cas9 mRNA (1000 ng/μl ...
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bioRxiv - Developmental Biology 2022Quote: ... Lysates were then incubated at 30°C for 30 minutes with lambda protein phosphatase (NEB).
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bioRxiv - Biochemistry 2021Quote: Pulldowns of MBP-tagged fusion proteins were performed using an amylose resin (New England Biolabs). Appropriate amounts of (see Fig ...
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bioRxiv - Biochemistry 2021Quote: ... 1μL m6A antibody per sample was coupled to pre-washed Protein G Magnetic Beads (NEB) in 1x reaction buffer (150mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... Immobilized proteins were deglycosylated with endoH in 0.5% SDS and 40mM DTT (New England Biolabs) overnight at 37°C according to the manufacturer.
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bioRxiv - Cell Biology 2022Quote: ... and either incubated with buffer control or lambda protein phosphatase (λ) (New England Biolabs; P0753) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2019Quote: ... per 1µg protein for 1 hour at 30°C in PMP buffer (New England Biolabs) plus 1mM MnCl2 ...
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bioRxiv - Neuroscience 2019Quote: ... protein samples were treated with peptide-N-Glycosidase F (PNGase) (New England Biolabs, Whitby, ON) following manufacturers guidelines ...
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bioRxiv - Developmental Biology 2020Quote: ... which was injected into one-cell state embryos together with Cas9 protein (New England Biolabs). Mutant animals were identified by PCR using the primers ...
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bioRxiv - Microbiology 2021Quote: ... Protein purification was done in eight steps: (1) amylose resin high flow (New England Biolabs) chromatography using a step elution from 0 mM to 10 mM maltose in Tris buffer A (20 mM Tris/HCl ...
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bioRxiv - Cell Biology 2019Quote: ... were precleared using 40μl of Protein-A Magnetic beads (New England Biolabs Cat No-S1425S) to remove non-specific components from the lysate ...
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bioRxiv - Microbiology 2021Quote: ... The protein expression level was evaluated in four T7 expression strains: T7 Express (C2566, NEB), T7 Express with LysY and lacIq (C3013 ...
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bioRxiv - Molecular Biology 2021Quote: ... IgG (Epredia™, NC748P) or 1X PBS was incubated with protein A beads (NEB, S1425S) with agitation for 1 hour at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... to visualize the bands alongside a color prestained protein broad range standard (New England Biolabs).
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bioRxiv - Developmental Biology 2021Quote: Injection mixes for CRISPR mediated manipulations consisted of 650 ng/ul dCas9 protein (NEB, M0652) or Cas9 protein (NEB ...
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bioRxiv - Genetics 2019Quote: ... sgRNAs were mixed with Cas9 protein (gift from Darren Gilmour, M0646M NEB, or B25641 invitrogen) and co-injected into 1-cell stage embryos using a microinjector (Eppendorf) ...
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bioRxiv - Cell Biology 2020Quote: ... 30 μg of microsomal proteins were treated with EndoH-Hf (New England Biolabs, Ipswich, MA) for 4 h at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Ribonucleoprotein (RNP) complexes were formed from sgRNA and recombinant Cas9 2NLS protein (New England Biolabs) mixed at a sgRNA to Cas9 ratio of 4.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... The lambda protein phosphatase procedure was carried out following the manufacturer’s guidelines (New England Biolabs). The sample was incubated at 30°C for 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... PURExpress-derived protein was mixed in a reaction containing 1x NEBuffer 2.1 (New England Biolabs), three-fold diluted PURExpress-derived protein ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was further purified on a 5-10 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Bioengineering 2023Quote: The heterologous protein MBPeGFP was purified using NEBExpress Ni spin columns (NEB, Ipswich, MA, USA) according to manufacturer recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... the protein coding sequences were ordered synthetically with Part 3b type BsaI overhangs (NEB R3733) and cloned into the entry vector with BsmBI (NEB R0739) ...
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TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... Chromatin was pre-cleared with 20 μl Protein A magnetic beads (New England Biolabs, S1425S) for 1.5 h and 100 μL of the solution was saved as an input control ...
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bioRxiv - Cell Biology 2023Quote: ... cells were lysed in 1x NEBuffer for Protein MetalloPhosphatases (New England BioLabs; supplied with λPP), with 0.5% NP-40 and 0.01 mg/mL leupeptin ...
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bioRxiv - Developmental Biology 2023Quote: ... The protein products were purified using the NEBExpress Ni Spin Columns (New England Biolabs, S1427S). The concentration of the purified products was quantified using the Qubit protein assay kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were determined via SDS–PAGE and Coomassie staining using BSA (New England Biolabs) as a standard ...
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bioRxiv - Molecular Biology 2023Quote: RNA-protein complexes were isolated with oligo[dT)]25 magnetic beads (New England Biolabs, S1419S). Briefly ...
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bioRxiv - Immunology 2023Quote: ... For embryo microinjection the annealed sgRNA were each complexed with Cas9 protein (New England Biolabs) at room temperature for 10min in injection buffer (final concentrations ...
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bioRxiv - Biophysics 2023Quote: ... MBP-HNRNPH2 proteins were purified by affinity chromatography using amylose resin (#E8021, New England BioLabs) and eluted with buffer containing 20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... the immunity protein genes were amplified by PCR using Phusion polymerase (New England Biolabs, UK) with the following primers ...
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bioRxiv - Bioengineering 2023Quote: ... The luciferase protein expressed in NiCo21(DE3) competent Escherichia coli (New England Biolabs, MA, USA) was purified using a Ni-NTA agarose resin column (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... All aliquots were supplemented with 10X NEBuffer for Protein MetalloPhosphatases (PMP) and 10X MnCl2 (NEB). The first aliquot (Untreated control ...
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bioRxiv - Physiology 2024Quote: ... 20 mg of protein from each sample was subjected to PNGase F treatment (NEB #P0704) to remove N-linked oligosaccharides prior to SDS-PAGE ...
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bioRxiv - Neuroscience 2024Quote: ... 30 µl of protein A or G paramagnetic beads (73778S or 9006S; New England Biolabs) were washed and then complexed with primary antibodies or naïve rabbit IgG at 4°C with end-over-end rotation ...
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bioRxiv - Biochemistry 2023Quote: ... protein was combined with 1.5x molar excess of SNAP-Surface Alexa488 dye (NEB, Cat# S9129S). SNAP dye labeling was performed in buffer containing 20 mM Tris [pH 8.0] ...
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bioRxiv - Biochemistry 2024Quote: ... Soluble protein fractions were mixed with an amylose resin column (New England BioLabs, Beverly, MA) pre-equilibrated with buffer A ...
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bioRxiv - Biochemistry 2023Quote: ... Initial protein-level deglycosylation was performed using 250 U of Endo H (New England Biolabs) for 5 µg trimer ...