Labshake search
Citations for New England Biolabs :
1001 - 1050 of 8872 citations for RPA kit reagent since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... the Monarch® Genomic DNA Purification Kit (NEB, Cat# T3010) was used (10 µl Proteinase K ...
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bioRxiv - Genetics 2023Quote: ... Libraries were prepared using Ultra II RNA library kit (NEB) and samples were sequenced on the NovaSeq 6000 platform (Illumina ...
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bioRxiv - Biochemistry 2024Quote: ... using a Q5 Site-Directed Mutagenesis Kit (New England Biolabs), or by nicking mutagenesis32,60 ...
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bioRxiv - Immunology 2023Quote: ... Libraries were prepped following NEBNext Immune Sequencing Kit’s protocol (NEB), and qPCR was used to determine cycling conditions prior to plateau ...
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bioRxiv - Synthetic Biology 2023Quote: ... When using the PURExpress® kit (E6800S, New England Biolabs), we adhered to the manufacturer’s protocol and incubated our sample at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The mRNA was purified by Monarch RNA Cleanup Kit (NEB). Purified mRNAs were stored at 2-6 μg/μl concentration and were diluted prior to injection with Femtojet to 100-500 ng/μl.
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bioRxiv - Cell Biology 2023Quote: ... using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB; E2621L). The retroviral construct containing farnesylation signal-GFP was purchased from addgene (21836) ...
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bioRxiv - Developmental Biology 2023Quote: ... sgRNAs were synthesized using the HiScribe T7 kit (NEB, E2050S) and purified by the RNA purification Kit (Zymogen ...
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bioRxiv - Immunology 2023Quote: ... Using the NEBridge® Golden Gate Assembly Kit (NEB, E1602), individual fragments were assembled to full-length RBD gene segments ...
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bioRxiv - Developmental Biology 2023Quote: ... using the Q5 site directed mutagenesis kit (NEB, Ipswich, MA) and primers RA1318 and RA1063 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The ProtoScript First Strand cDNA Synthesis Kit (New England Biolabs) was used for the synthesis of cDNA using the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... and extracted their RNA using a miniprep kit (NEB, T2010S), performed reverse transcription (NEB ...
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bioRxiv - Genomics 2023Quote: ... NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) was used to prepare indexed sequencing libraries following the manufacturer supplied protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Luna Universal One-Step RT-qPCR Kit (NEB), and then analyzed with QuantStudio Design & Analysis Software v.1.5.1 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... or the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). We verified the sequences of plasmid DNAs purified from transformed E ...
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bioRxiv - Synthetic Biology 2024Quote: ... RNA was purified using the MonarchR RNA Cleanup Kit (NEB). The integrity of the purified RNA was determined by running RNA in a 1% agarose gel ...
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bioRxiv - Bioengineering 2024Quote: ... The LunaScript Primer-Free RT Master Mix Kit (NEB E3025S) was used to generate cDNA per manufacturer protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... NEBuilder® HiFi DNA Assembly Kit (catalog no. E2621L, NEB) was used to clone the sequence of interest into plasmid backbone.
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bioRxiv - Genetics 2023Quote: ... and concentration determined using NEBNext Lib Quant Kit (E7630, NEB). Libraries were sequenced with an Illumina NextSeq550 with 42bp per read (total of 84bp ...
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bioRxiv - Biophysics 2024Quote: ... and R88 using the Q5 site-directed mutagenesis kit (NEB). Giant spheroplasts were generated as described previously (Martinac et al. ...
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bioRxiv - Biophysics 2024Quote: ... using the NEBuilder HiFi DNA Assembly kit (New England Biolabs); by strictly following the protocols recommended by the manufacturers ...
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bioRxiv - Genomics 2024Quote: ... Ultra FS II library kit (New England BioLabs, Ipswich, MA) was used ...
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bioRxiv - Genetics 2024Quote: ... After the library quantification via NEBNext Library Quant Kit (NEB), paired-end sequencing was performed on the tiles of each region with a sequencing depth of ∼2 million reads per tile using an Illumina NextSeq 500 instrument via a NextSeq 500/550 High Output Kit v2.
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bioRxiv - Developmental Biology 2024Quote: The PCR-based Q5 Site-Directed Mutagenesis Kit (NEB, #E0554S) was used to perform truncation (constructs L-P ...
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bioRxiv - Microbiology 2024Quote: ... Using ProtoScript First Strand cDNA Synthesis Kit (New England BioLabs), RNA is denatured and cDNA was synthesized ...
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bioRxiv - Microbiology 2023Quote: ... and Luna Universal qPCR Master Mix kit (New England Biolabs). Reactions were performed as technical replicates within each of three biological replicates ...
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bioRxiv - Microbiology 2024Quote: ... Isolation was performed using Monarch Plasmid Miniprep Kit (NEB, UK).
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bioRxiv - Microbiology 2024Quote: ... coli strain carrying the plasmid (Monarch Mini-Prep kit, NEB) and transformed it into electrocompetent K ...
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bioRxiv - Immunology 2024Quote: ... The HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) was used to transcribe the template DNA per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were isolated using Monarch Plasmid Miniprep kit from NEB and linearized using restriction enzyme by NEB as per manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... purified using the Monarch® DNA gel extraction kit (NEB) and subjected to Sanger Sequencing for ORF sequence-verification prior to codon-optimization at Genscript and subcloning ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were prepared according to manufacturer’s instructions using the following kits: NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® and NEBNext® Multiplex Oligos for Illumina® (New England BioLabs) and pooled in equimolar quantities ...
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bioRxiv - Molecular Biology 2022Quote: ... Ribosomal RNA was depleted of using the NEBNext® rRNA Depletion Kit and cDNA libraries were prepared using the NEBNext® Ultra™ Directional RNA Library Prep Kit for Illumina® (NEB, Ipswich MA). RNA was barcoded using the NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Plant Biology 2020Quote: ... mRNA was isolated with Oligo-dT Beads from NEB and RNAseq libraries were constructed with the Directional Kit from NEB. Sequencing was performed at IPS2 Paris ...
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bioRxiv - Synthetic Biology 2021Quote: ... The reaction was set up using a commercial kit (PURExpress NEB) according to user guidelines ...
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bioRxiv - Cancer Biology 2021Quote: ... The NEBNext ultra II RNA library prep kit (New England Biolabs) was used to generate poly(A)+ libraries ...
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bioRxiv - Microbiology 2020Quote: ... the overhanging ends were blunted using a blunting enzyme kit (NEB) and an A-tail was added with Taq DNA polymerase (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... using the NEBuilder HiFi DNA Assembly Cloning Kit (New England BioLabs). Allelic replacement of the hlyA open reading frame by this constructs ...
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bioRxiv - Developmental Biology 2021Quote: ... Mutagenic primers and the Q5 site-directed mutagenesis kit (NEB E0554S) were used to generate the BD-Chinmonon-stop and the CG11180/Chigno-sim* mutant ...
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bioRxiv - Cell Biology 2019Quote: ... The NEBNext Ultra II RNA Library Prep Kit (New England Biolabs) was used to generate TruSeq-barcoded RNA-Seq libraries ...
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bioRxiv - Evolutionary Biology 2019Quote: ... using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs) and the online tool NEBasechanger to design the mutagenesis primers ...
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bioRxiv - Microbiology 2020Quote: ... The ChiZ overexpression construct was engineered using the NEBuilder kit (NEB), according to the manufacturer’s instructions with primers PA311 + PA312 and LW043 + LW044 and the pBRplac-lacI derivative ...
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA fragment ends were repaired using Quick Blunting™ kit (NEB), purified with QIAquick column (Qiagen ...
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bioRxiv - Developmental Biology 2021Quote: ChIP-seq libraries were made following the NEBnext UltraII kit (NEB) and associated protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... cDNA libraries were prepared using the E7765L mRNA kit (NEB next). Paired-end read sequencing was performed using the HiSeq2500 instrument ...
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bioRxiv - Neuroscience 2022Quote: ... Assembly was done using NEB HiFI DNA assembly kit (NEB E5520) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Ribosomal RNA was depleted using the NEBNext rRNA depletion kit (NEB) following the product instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... MeRIP was performed using the EpiMark N6-methyladenosine Enrichment Kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and the NEBuilder HiFi DNA assembly cloning kit (New England Biolabs), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... ARF1 point mutations were generated by Quick-change Mutagenesis kit (NEB) and KLD reactions ...