Labshake search
Citations for New England Biolabs :
1001 - 1050 of 10000+ citations for Mouse Switch associated protein 70 SWAP70 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... samples containing the same amount of protein (50 µg/sample) were processed for endoglycosidase-H (Endo-H, NEB) digestion using 5 milliunits Endo-H for 16h at 37°C ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 µg antibody (Table. S3) and 50 µl protein A magnetic beads (New England Biolabs, Cat. No. S1425S) were added to the supernatant and incubate overnight at 4 degree Celsius ...
-
bioRxiv - Cell Biology 2019Quote: ... 5-10 µg of total protein (9 µl of lysate) were treated with Endo Hf (New England Biolabs) for 1 h at 37°C ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 40 μl of filtered double-distilled water and 20 μl of Blue Protein Loading Dye (New England Biolabs) were added to the beads ...
-
bioRxiv - Cell Biology 2019Quote: ... The recombinant fusion proteins were purified via affinity chromatography from bacterial extracts using amylose resin (New England Biolabs) according to manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... σA-FLAG and RbpA-FLAG proteins were prepared by PCR using Q5® High-Fidelity DNA Polymerase (NEB) with primers #3130 + #3131 (HelD) ...
-
bioRxiv - Biochemistry 2021Quote: ... His tagged proteins were eluted with lysis buffer containing 400mM imidazole and bound immediately to amylose resin (NEB) for 1hr ...
-
bioRxiv - Microbiology 2021Quote: ... Standard curve was prepared using SARS-CoV-2 Positive Control plasmid containing full nucleocapsid protein (N gene) (NEB) and used to quantify copies of N gene in organoid samples ...
-
bioRxiv - Microbiology 2021Quote: ... A small fraction of the refolded MBP-ACE2NTD fusion protein was further purified by amylose magnetic beads (NEB) according to the protocol provided by the manufacturer.
-
bioRxiv - Molecular Biology 2021Quote: ... EcoRI sites underlined) and different concentration of recombinant His-SET8 protein (0 to 1.4 μM, New England Biolabs) were incubated for 10 min on ice in 1× GRB binding buffer [20 mM HEPES pH 7.5 ...
-
bioRxiv - Microbiology 2022Quote: ... Either 3-5 µL of Color Prestained Protein Standard-Broad Range (11-245 kDa) (New England Biolabs, P7712) or 1-3 µL of PageRuler Prestained Protein Ladder (10-180 kDa ...
-
bioRxiv - Microbiology 2022Quote: ... 1-20 μg of the Env protein was mixed with 1 μl of Glycoprotein Denaturing Buffer (NEB, 10×) and H2O (if necessary ...
-
bioRxiv - Cell Biology 2022Quote: ... residual ATP from the protein preparation was depleted using apyrase by mixing 4.5 µl of 500 nM protein with a 0.5 µl of apyrase (NEB), and incubating for 30 minutes at room temperature ...
-
bioRxiv - Microbiology 2024Quote: ... Samples were resolved on SDS-PAGE gels alongside a Blue Protein Standard Broad Range ladder (New England BioLabs). Bands were transferred onto nitrocellulose membranes and were blocked with a 5% (w/v ...
-
bioRxiv - Cell Biology 2024Quote: ... one of the immunoprecipitated samples was treated with 400 units of Lambda protein phosphatase (New England Biolabs, P0753S) at 30 °C for 30 min ...
-
bioRxiv - Developmental Biology 2024Quote: ... the Gbait and dld gRNAs were combined at 4.8uM each with 20uM Lba Cas12a protein (New England Biolabs) and incubated for 10 minutes at 370C ...
-
bioRxiv - Molecular Biology 2023Quote: NSD1/2 WT and different cancer mutants (3.4 μM) were mixed with recombinant H3.1 protein (1 μg) (purchased from NEB) or recombinant H3.1 mononucleosomes in methylation buffer (50 mM Tris/HCl ...
-
bioRxiv - Bioengineering 2023Quote: ... 0.27 nmol ErCas12a/LbCas12aU protein and 0.45 nmol crRNA were assembled in 1 X Nuclease Reaction Buffer (NEB). The protein and RNA were mixed and incubated for 10 minutes at room temperature and used for transformation of embryogenic C ...
-
bioRxiv - Biochemistry 2023Quote: ... and then deglycosylated and dephosphorylated overnight at 37 °C with the protein deglycosylation mix II (New England Biolabs) and Quick CIP (New England Biolabs) ...
-
bioRxiv - Microbiology 2023Quote: ... samples containing the same amount of protein (20 µg/sample) were processed for endoglycosidase-H (Endo-H, NEB) digestion using 5 milliunits Endo-H for 16h at 37°C ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were expressed and purified from 2 to 4 liters of Escherichia coli NiCo21(DE3) (New England Biolabs) as previously described (24) ...
-
bioRxiv - Genetics 2023Quote: Total protein extracts were prepared with 2.106 cells re-suspended in 50 μL of loading buffer (B7709S, Biolabs) containing 1% SDS ...
-
bioRxiv - Cell Biology 2023Quote: ... Purified sgRNAs targeting tlnrd1 and slc45a2 were pooled separately and complexed with recombinant Cas9 protein (New England Biolabs) in vitro using 300mM KCl buffer for 5 minutes at +37°C ...
-
bioRxiv - Microbiology 2023Quote: ... E protein glycosylation status was determined by treating RVP lysates with PNGase F (Cat#P0704S, New England Biolabs) for 3 hours at 37°C ...
-
bioRxiv - Plant Biology 2024Quote: ... The proteins were purified using the amylose resin affinity purification method by following the manufacturer’s recommendations (E8021L, NEB). Briefly ...
-
bioRxiv - Developmental Biology 2021Quote: ... the RNA samples isolated by PureLink Mini kit were subsequently purified by Magnetic mRNA Isolation kit (New England Biolabs, USA). mRNA concentration was estimated by Qubit RNA HS Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2021Quote: ... amplicons were gel purified with the InnuPREP Gel Extraction Kit (Analytik Jena) or the Monarch DNA Gel Extraction Kit (NEB), and submitted for Sanger sequencing (Eurofins Genomics) ...
-
bioRxiv - Developmental Biology 2020Quote: ... RNA was extracted using the Qiagen RNeasy Micro kit and libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England BioLabs). All libraries were sequenced on an Illumina HiSeq 2500 with 100 bp single-end reads.
-
bioRxiv - Molecular Biology 2021Quote: ... Adaptor and index primers were added to the sheared DNA fragments using a next-generation sequencing library preparation kit (NEBNext Ultra DNA Library Prep Kit for Illumina; NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted using illustra RNAspin Mini isolation kit (Cytiva) or Monarch Total RNA Miniprep Kit (New England Biolabs) subsequent to spheroblast construction using 10 U of Zymolyase (ZYMO Research ...
-
bioRxiv - Developmental Biology 2019Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II library prep kit with the NEB Unique Dual Index primer set kit (New England Biolabs). Samples were sequenced at the Northwestern University NuSeq Core Facility on an Illumina HiSeq 4000 on single-end 50 bp mode ...
-
bioRxiv - Microbiology 2019Quote: ... using Agilent High Sensitivity DNA kit in a Bioanalyzer 2100 instrument then quantitated using qPCR based NEBNext Library Quant Kit for Illumina (New England Biolabs) with a Piko-Real Real-Time PCR System (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2019Quote: ... Total RNA libraries were generated using NEBNext rRNA Depletion kit combined with NEBNext Ultra II Direction RNA Library Prep kit for Illumina (New England Biolabs). We used 100 ng of starting RNA material for input and pre-sorted synaptosomes and 1-5 ng for vGLUT1+ sorted synaptosomes which corresponds to ~100 Mio synaptosomes collected per P3 fractions ...
-
bioRxiv - Cancer Biology 2021Quote: ... Samples with RIN value higher than 8 were processed using the NEBNext poly(A) mRNA Magnetic Isolation kit and the NEBNext Ultra II RNA Library Prep kit for Illumina (New England Biolabs), following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... PCR/reaction cleanup and gel extraction were conducted with the Monarch PCR and DNA cleanup kit and Monarch DNA gel extraction kits (NEB). E ...
-
bioRxiv - Microbiology 2021Quote: ... cholerae and phages was extracted using commercially available kits (Qiagen DNeasy Blood and Tissue or Monarch genomic DNA purification kit (New England BioLabs). For preparation of phage DNA ...
-
bioRxiv - Microbiology 2022Quote: ... After enrichment of mRNA using the Universal Ribodepletion Kit cDNA preparation was performed with the Next Ultra Directional Library Preparation Kit for Illumina (NEB). The cDNA was sequenced on HiSeq 3000 (Illumina ...
-
bioRxiv - Cell Biology 2022Quote: ... 60 ng of total RNA was used for library preparation using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNext rRNA Depletion Kit (New England Biolabs). The libraries were paired-end sequenced (2 × 75 bp ...
-
All-trans retinoic acid induces synaptopodin-dependent metaplasticity in mouse dentate granule cellsbioRxiv - Neuroscience 2021Quote: ... and RNA was consecutively isolated using a column based RNA isolation kit according to the manufacturer’s instructions (Monarch® Total RNA Miniprep Kit; #T2010S New England Biolabs). Strand specific cDNA library preparation from polyA enriched RNA (150 bp mean read length ...
-
bioRxiv - Immunology 2019Quote: ... The culture supernatant was projected to perform luciferase assay with Gaussia Luciferase assay kit and Cypridina Luciferase assay kit (NEB) following their description.
-
bioRxiv - Systems Biology 2021Quote: Bulk RNA-seq libraries were prepared from 1000 ng of RNA using the NEBNext Ultra RNA Library Prep kit for Illumina or the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) as per the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Enrichment of mRNA was done using the Universal Ribodepletion Kit followed by Next Ultra Directional Library Preparation Kit for Illumina (NEB). The cDNA was sequenced on HiSeq™ 2500 (Illumina ...
-
bioRxiv - Genomics 2021Quote: ... Libraries were constructed from 1 µg of total RNA using Illumina’s TruSeq stranded mRNA Library Preparation Kit or from 500 ng total RNA using NEBNext Ultra II RNA -polyA+ (mRNA) Library Preparation Kit (New England Biolabs) following the manufacturer’s description.
-
bioRxiv - Genetics 2021Quote: ... RNA samples were depleted of rRNA using the QIAseq FastSelect RNA Removal Kit then processed into libraries using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Samples were recovered by phenol/CHCl3/isoamyl alcohol extraction twice and purified as per kit instruction using Monarch PCR and DNA cleanup Kit (New England BioLabs). DNA was suspended in 200 ul pre-warm (55°C ...
-
bioRxiv - Developmental Biology 2021Quote: ... or deletions in full-length Gpr161 were generated using Quikchange site-directed mutagenesis kit (Stratagene or Q5 Mutagenesis Kit (NEB).
-
bioRxiv - Molecular Biology 2021Quote: ... RNA-sequencing libraries were prepared with a NEBNext rRNA Depletion Kit and an ENBNext Ultra Directional RNA Library Prep Kit (New England Biolabs). Then ...
-
bioRxiv - Genomics 2022Quote: MNase-seq libraries were prepared from purified nucleosomal DNA using the kit NEBNext Ultra DNA Library Prep Kit for Illumina (cat. # E7370L New England BioLabs) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: RNA was purified by silica-based SPE using Monarch RNA Cleanup Kits (for in vitro assays) or Total RNA Miniprep Kit for intracellular samples (NEB).
-
bioRxiv - Plant Biology 2022Quote: ... Total RNA was isolated using Qiagen RNeasy Plant Mini Kit and cDNA was synthesised using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs). SYBR Green Power Up (Thermofisher ...