Labshake search
Citations for New England Biolabs :
1001 - 1050 of 1891 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2022Quote: ... 3 mM MgCl2 and 2 μL (10 U) RNase H (NEB, Cat# M0297S) in order to digest poly(A ...
-
bioRxiv - Molecular Biology 2022Quote: ... and 10 units T4 Polynucleotide Kinase (PNK) enzyme (New England Biolabs, cat#M0201S) for 2 hours at 37°C in 1X T4 PNK buffer (New England Biolabs ...
-
bioRxiv - Molecular Biology 2022Quote: ... all the buffers above were supplemented with 10 mM ribonucleoside-vanadyl complex (NEB) to inhibit ribonucleases ...
-
bioRxiv - Molecular Biology 2022Quote: ... 1 µL was used in a 10 µL Kinase/Ligase/DpnI (KLD, NEB) reaction and incubated at room temperature for 10-20 min ...
-
bioRxiv - Synthetic Biology 2022Quote: ... chemically competent DH5α cells or NEB 10-beta competent cells (NEB, Cat#C3019). Constructs were selected on LB plates (1% Tryptone ...
-
bioRxiv - Molecular Biology 2024Quote: ... and resuspending cells in 950 uL 10-beta/Stable Outgrowth Medium (NEB, B9035S). Serial dilutions of 10 uL were immediately spread onto Ampicillin selection plates to assay transformed library complexity (>= 10,000x) ...
-
bioRxiv - Molecular Biology 2024Quote: ... were added with 1.5 µL of 10× PNGase F buffer (New England Biolabs) in the sample by adjusting with Milli-Q H2O to total 15 µL ...
-
bioRxiv - Cell Biology 2024Quote: ... and 10 mM ribonucleoside vanadyl complex (RVC; S1402S; New England Biolabs; Ipswich, MA) for 10–20 min in a 37°C water bath ...
-
bioRxiv - Genomics 2024Quote: ... After adding 10 µL of 20 mg/µL proteinase K (New England Biolabs), proteins were degraded for 30 minutes at 55°C followed by an overnight incubation at 68°C with shaking to reverse crosslinks ...
-
bioRxiv - Molecular Biology 2024Quote: ... Adaptor ligation was performed with a 1:10 dilution of adaptor (NEB, E6440S). For DNA purification ...
-
bioRxiv - Systems Biology 2023Quote: ... The transformation into NEB 10-beta electrocompetent E.coli (New England Biolabs, Ipswitch, MA) was performed slightly differently ...
-
bioRxiv - Systems Biology 2024Quote: ... 2-10 µg plasmid DNA was digested with BsaI-HF-v2 (NEB R3733) in a 100 µl reaction for 4 h at 37°C and then spin-purified.
-
bioRxiv - Microbiology 2023Quote: ... The TRIzol-isolated RNA was treated with 10 U RNase-free DNaseI (NEB) for 30 min at 37°C ...
-
bioRxiv - Biochemistry 2023Quote: ... 10 μg genomic DNA was digested with 20 units Sty I (NEB, R3500S) at 37 °C for 3 h ...
-
bioRxiv - Systems Biology 2023Quote: ... Two transformations into NEB 10 beta electrocompetent cells (New England Biolabs, Ipswitch, MA) were performed as described above ...
-
Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... digested in buffer containing 10 mM DTT with Proteinase K (New England Biolabs), and expanded in ultrapure water as for the cell work ...
-
bioRxiv - Molecular Biology 2023Quote: ... and digested with 0.5 µl (10 U) EcoRI and NotI (New England BioLabs). The same restriction enzymes were used to digest the 3’ fragment and to ligate the 3’ fragment and 5’-pGEM-T easy plasmid and the resulting vector containing PpRossmann fold 5’−3’-pGEM-T was transformed into E ...
-
bioRxiv - Cell Biology 2022Quote: ... for 10 mins at 100°C and then resuspended in EndoH (NEB, P0702S) for 1.5 hours at 37°C ...
-
bioRxiv - Biochemistry 2022Quote: ... Each sample was mixed with 10 µL 10x MNase buffer (New England Biolabs), 1 µL 100x BSA (New England Biolabs) ...
-
bioRxiv - Microbiology 2022Quote: pWR1566 was purified from laboratory E.coli strain NEB 10-beta (New England Biolabs) and used to transform V.cholerae strain WR2700 using electroporation ...
-
bioRxiv - Plant Biology 2022Quote: ... with 10 units restriction enzyme (BsaI-HFv2, NEB or BpiI, Thermo Fisher Scientific) and 10 units T4 DNA ligase (HC ...
-
bioRxiv - Cell Biology 2023Quote: ... 10 mM DTT) or 5 units of RNASEH1 enzyme (M0297, New England Biolabs) for 30 mins at 37 °C ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... 10 U of both enzymes EcoRI-HF and HindIII-HF (New England Biolabs), 5.27 μl nuclease-free water (Sigma ...
-
bioRxiv - Genetics 2023Quote: ... and transformed into NEB 10-beta electrocompetent E.coli (New England BioLabs Cat. #3020). Plasmids were purified using a Qiagen Plasmid Midi Kit (Qiagen Cat ...
-
bioRxiv - Microbiology 2022Quote: ... 0.25 μL of dNTPs at an original concentration of 10 μM (NEB, N0447L), 2.5μL of 5X Maxima H Minus Reverse Transcription Buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... 0.05% Tween-20) containing 10 µMnt of circular ssDNA (PhiX174 virion DNA, NEB) immobilised on magnetic Streptavidin-coated beads (Dynabeads M-280 ...
-
bioRxiv - Biochemistry 2023Quote: ... 4 U/mL creatine kinase) containing 10 µMnt cssDNA (NEB, PhiX virion DNA) was supplemented with Rad51 (5 µM ...
-
bioRxiv - Genomics 2023Quote: ... 0.75 µl of Thermopol Reaction buffer (New England Biolabs, cat. no. B9004S, 10×) and 0.5 µl of Sulfolobus DNA polymerase IV (New England Biolabs ...
-
bioRxiv - Genetics 2023Quote: ... 10 μl of Q5 Hot Start High-Fidelity 2x Master Mix (NEB M0494S), 1 μl of 10 μM Primer 1 (5’-ATGAAGCTACTGTCTTCTATCGAACAAGC-3’ ...
-
bioRxiv - Cell Biology 2024Quote: ... Reactions (10 µl) were performed Luna Universal qPCR Master Mix (New England Biolabs). mRNA abundance for each gene was determined relative to GAPDH mRNA using the 2−ΔΔCt method ...
-
bioRxiv - Cell Biology 2024Quote: ... Mutagenic PCR products were then digested with 10 U DpnI (New England Biolabs) at 37°C for 30 min to eliminate template DNA ...
-
bioRxiv - Cell Biology 2024Quote: ... The Color Protein Standard Broad Range (10–250 kDa) (New England BioLabs Inc.) was used as protein molecular weight ladder ...
-
bioRxiv - Molecular Biology 2024Quote: ... mixed with 1 μL (10 units) of T4 Polynucleotide Kinase (NEB, Cat #M0201L), 5 μL T4 Polynucleotide Kinase Reaction Buffer (NEB ...
-
bioRxiv - Microbiology 2024Quote: ... Enco) by incubating with 10 U of T4 polynucleotide kinase (New England Biolabs) at 37 °C for 1 hour and cleaned using G50 columns (Microspin™ G-50 Columns ...
-
bioRxiv - Cell Biology 2024Quote: ... coli Lysis Buffer (1x TBS, 1x Protease Inhibitor Cocktail, 10 μl Benzonase [NEB] ...
-
bioRxiv - Molecular Biology 2024Quote: ... CD44_7k – BamHI / T7) and 10% v/v CutSmart Buffer (New England Biolabs, USA) at 37°C for 4 hours ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 5 mM BME) onto a 10 ml amylose column (New England Biolabs). The amylose column was washed with lysis buffer ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 10 µg of purified RNA was immobilized on streptavidin magnetic beads (NEB) for 8 h at 4°C under orbital rotation in binding buffer (24 mM KCl ...
-
bioRxiv - Neuroscience 2024Quote: ... The lysates were then treated with 10 μl DNase I (New England Biolabs) and incubated at 4°C for 1 hour on a rotator followed by centrifugation at 13,000 rpm ...
-
bioRxiv - Molecular Biology 2024Quote: ... 10 µg of isolated RNA was treated with DNase I (NEB, Cat: M0303S) to remove any contaminating genomic DNA ...
-
bioRxiv - Plant Biology 2024Quote: ... 10 μg of gDNA was digested with SacI restriction endonuclease (New England Biolabs). Digested samples were electrophoresed on a 1% agarose gel stained with SYBR Safe (Invitrogen ...
-
bioRxiv - Genetics 2024Quote: ... a 10 µL reaction containing 0.2 µL AseI (New England BioLabs, MA, USA) and 1 µL CutSmartTM r3.1 Buffer (New England BioLabs ...
-
bioRxiv - Molecular Biology 2023Quote: ... using the manufacturer’s protocol or with radioactive labeling mix (1 µL 10x PNK buffer, NEB, 1 µL of 100 µM gapmer, 1 µL T4 PNK, NEB, 1 µL γ-32P-ATP, Hartmann, 6 µL ddH2O) for 40 min at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: ... 3 µg of purified DNA was digested with 10 units I-SceI (NEB, R0694), 10 units I-CeuI (NEB ...
-
bioRxiv - Genetics 2021Quote: ... About 10 ng of genomic DNA was fragmented using the NEBNext dsDNA fragmentase (NEB) and sequencing libraries were generated using the NEBNext Ultra DNA Library prep kit (NEB ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... This 20 μL 10-cycle PCR reaction was performed using Q5 polymerase (NEB M0491L) following the manufacturer’s guidelines ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 500 ng DNA were digested with 10 units of CviQI (NEB, cutting site G’TAC) at 25°C for 2 h ...
-
bioRxiv - Molecular Biology 2020Quote: ... Then 1.5 µl of 10 mM stock of the m7GpppA (S1405L, New England Biolabs) was added to crystal slurry and the mix was loaded on a 60 µm grid made from nylon (NY6004700 ...
-
bioRxiv - Molecular Biology 2021Quote: ... RCA products were debranched using 10 units T7 Endonuclease/μg DNA (New England Biolabs) for 15 minutes at 37 °C ...
-
bioRxiv - Molecular Biology 2021Quote: ... In vitro translation was carried out in 10 µL using the PURExpress kit (NEB) according to the commercial protocol in the presence of a mix of 32P-radiolabeled cspA mRNA (200000 cpm/µL ...