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Citations for New England Biolabs :
1001 - 1050 of 4333 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... followed by annealing at RT for 2h and subsequently ligation with T4 ligase (NEB) into the BsmBI-digested (Fermantas ...
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bioRxiv - Systems Biology 2021Quote: ... PE2 indexing barcodes were then added by amplifying 2 microliters of the previous reaction with forward primer P1_outer and reverse primers PE2_outer_SIC69 and PE2_outer_SIC70 (Supplementary file 6) for 5 cycles at an annealing temperature of 66C followed by 5 cycles with no annealing step (NEB Q5) and then purified with the Monarch PCR kit.
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bioRxiv - Systems Biology 2021Quote: ... P1 indexing barcodes were added using forward primers P1_inner_A through P1_inner_D and reverse primer P1_inner_nested_rev (Supplementary file 6) for 5 cycles at an annealing temperature of 55C followed by 5 cycles with no annealing step (NEB Q5). PE2 indexing barcodes were then added by amplifying 2 microliters of the previous reaction with forward primer P1_outer and reverse primers PE2_outer_SIC69 and PE2_outer_SIC70 (Supplementary file 6 ...
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bioRxiv - Cell Biology 2020Quote: ... 3 µg of purified DNA was digested with 10 units I-SceI (NEB, R0694), 10 units I-CeuI (NEB ...
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bioRxiv - Genomics 2020Quote: ... and followed by incubation with 3 μl (1 U/μl) of USER enzyme (NEB) at 37°C for 20 min ...
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bioRxiv - Neuroscience 2021Quote: ... with 3’-O-Me-m7GpppG anti-reverse cap analog (ARCA) or ApppG cap (NEB) added at 8:1 to GTP for a capping efficiency of ∼90% ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 μg of RNA was resuspended in 6x Loading dye (Biolabs, cat. No B7024S) and separated on a 20% PA gel without urea run at 160 volts at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... adaptor-ligated fragments were treated with 3 µl of USER® Enzyme (NEB, USA) before selective PCR enrichment using Phusion® High-Fidelity DNA polymerase using Universal and index primers ...
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bioRxiv - Plant Biology 2021Quote: ... and ligated at a 3:1 amplicon:plasmid ratio with t4 DNA ligase (NEB, US) overnight at 16°C ...
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bioRxiv - Genetics 2022Quote: ... and ligating into the BbsI ligated U6:3 vector (T4 ligase NEB, BbsI NEB) (S3 Table) ...
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bioRxiv - Genetics 2022Quote: ... and ligating into the BbsI ligated U6:3 vector (T4 ligase NEB, BbsI NEB) (S3 Table) ...
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bioRxiv - Microbiology 2022Quote: ... 15 μl 20% SDS and 3 μl 20 mg/ml Proteinase K (NEB #P8107) was added to the sample and the mixture was incubated at 37 °C for 30 min with agitation at 1400 rpm to completely lyze the cells ...
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bioRxiv - Developmental Biology 2021Quote: ... custom NTP mix was prepared with 3’-O-Me-m7G cap analogue (60mM, NEB), GTP (75mM ...
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bioRxiv - Immunology 2020Quote: ... and 3’ end A-overhangs were added by NEBNext® dA-Tailing Module (NEB). The A-tailed products were subsequently cloned into pGEM®-T Easy vector (Promega ...
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bioRxiv - Genetics 2020Quote: ... remaining ssDNA oligonucleotides were digested by the addition of 3 μL exonuclease 1 (NEB) to 20 μL extracted DNA ...
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bioRxiv - Synthetic Biology 2020Quote: ... This was done in 50 μl reactions using 3 μl dual index primers (NEB), 135 ng purified PCR product from the previous reaction and 25 μl Q5 high fidelity master mix (NEB) ...
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bioRxiv - Physiology 2020Quote: ... 636 bp 3’ arm of homology) with the nicking enzyme Nb.Bsm1 (New England Biolabs) and purification from an alkaline-agarose gel ...
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bioRxiv - Molecular Biology 2022Quote: ... and/or 10 U of T4 PNK (wild-type or 3’ phosphatase minus, NEB). For RtcB ligation ...
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bioRxiv - Biochemistry 2022Quote: ... 3’A overhang were added to each fragment using the Klenow fragment (NEB #M0212M) and DNA was purified using MinElute PCR Purification Kit (QIAGEN #28006) ...
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bioRxiv - Genomics 2022Quote: ... Illumina sequencing adapters containing uracil hairpin loop structure and 3′ T overhangs (NEB, #E7337A) were ligated to the DNA by adding 150 units of Quick T4 DNA Ligase (NEB ...
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bioRxiv - Molecular Biology 2022Quote: ... 3’UTR mutant was obtained by using the Site Directed Mutagenesis (New England Biolabs). miR-19a ...
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bioRxiv - Plant Biology 2024Quote: ... was used for Level 1 and 3 assembly and SapI (New England BioLabs, USA) for Level 2 assembly ...
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bioRxiv - Molecular Biology 2024Quote: ... HR template was divided into 3 parts and each part was amplified by NEB Q5 polymerase using AJ51 & AJ52 oligonucleotides for part 1 ...
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bioRxiv - Biochemistry 2024Quote: ... crosslinked RNA fragments were dephosphorylated at their 3’-ends using T4-PNK (NEB, M0201S) and ligated to a pre-adenylated 3’-adapter (L3-App ...
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bioRxiv - Molecular Biology 2023Quote: ... these products were ligated to a 3’ adapter (1× T4 RNA ligase buffer (NEB), 10% PEG-8000 ...
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bioRxiv - Immunology 2022Quote: ... To digest remaining M1 tag forward oligo: 3 µl 20U/ µl Exonuclease I (NEB) was added and samples were incubated for 1 h at 37°C ExoI denaturation was performed for 5 min at 98°C ...
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bioRxiv - Cell Biology 2023Quote: ... and Exon 3 amplified using Q5® High-Fidelity DNA Polymerase (New England Biolabs). The following primers were used ...
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bioRxiv - Molecular Biology 2023Quote: ... free adapters were removed by adding 3 units of RecJf (New England Biolabs, M0264) and 1.7 units of 5ʹ deadenylase (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was ligated with 3′-adenylated adapters using T4 RNA Ligase 2 (NEB #MO351) for 4 hrs at 16°C ...
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bioRxiv - Microbiology 2023Quote: ... then barcoded RNA adapters were ligated to the 3′ end (T4 RNA Ligase, NEB). Samples were separated by SDS-PAGE and transferred to nitrocellulose membranes ...
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bioRxiv - Cell Biology 2024Quote: ... infrared IR800-dye conjugated 3’ adapters were ligated overnight using RNA ligase 1 (NEB) in a high-percentage PEG8000 buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... a 3′ RNA adapter was ligated onto the RNA with T4 RNA ligase (NEB). Protein-RNA complexes were run on an SDS-PAGE gel ...
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bioRxiv - Genomics 2024Quote: ... 264 nl of 3’ ligation brew (1× T4 RNA Ligase Buffer (New England Biolabs), 1 µM pre-adenylated 3’ linker (Integrated DNA Technologies) ...
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bioRxiv - Synthetic Biology 2024Quote: ... across 3 reactions) were double digested with NheI-HF (New England Biolabs, Cat# R3131L) and MfeI-HF (New England Biolabs ...
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bioRxiv - Developmental Biology 2024Quote: ... then assembled with the ckb-3 promoter via Gibson assembly (New England Biolabs, US).
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bioRxiv - Microbiology 2020Quote: ... Annealed oligos were mixed with 4 μL 6X loading dye (NEB, B7024S), loaded onto a lab-made 8% TBE gel (Acrylamide/Bis solution 37.5:1 ...
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bioRxiv - Genomics 2020Quote: ... the circularized DNA was incubated in 1x NEBuffer 4 reaction buffer (NEB) (50 mM potassium acetate ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM MgCl2 and 4 units of RNase Inhibitor Murine (NEB, M0314). After 30 min at room temperature ...
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bioRxiv - Systems Biology 2020Quote: ... a 4-cycle PCR was performed with OneTaq polymerase (New England Biolabs) in 200 reactions (125 μL/reaction) ...
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bioRxiv - Microbiology 2021Quote: ... The parental plasmid was digested for 4 h using Dpn1 endonuclease (NEB) at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... a mix of 4 μl First strand Synthesis Reaction Buffer (NEB-kit), 0.5 μl Murine RNase Inhibitor (NEB-kit ...
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bioRxiv - Genomics 2020Quote: ... 4 μL of 20 mg/mL RNase A (New England Biolabs #T3018L) is added to the RNase positive aliquot of non-lysed TSB from 1-gram cannabis flower homogenate ...
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bioRxiv - Developmental Biology 2021Quote: ... by incubating with DNA polymerase I Klenow (4 mL; New England Biolabs) for 90 min at 37C with rotation ...
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bioRxiv - Immunology 2022Quote: ... and subsequently incubated with 4 U of alkaline phosphatase (New England Biolabs) at 37°C for 30 min to hydrolyze unreacted NTPs ...
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bioRxiv - Developmental Biology 2024Quote: ... 47 ml water) and 4 µL 10mg/ml Proteinase K (P8108S, NEB). The samples were incubated for 8 hours at 55 °C followed by 10 min at 98 °C to inactivate the Proteinase K ...
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bioRxiv - Biochemistry 2024Quote: ... then subsequent addition of 4 units of proteinase K (New England Biolabs) and incubation at 55 °C for 30 min ...
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bioRxiv - Biophysics 2024Quote: ... 4 mM BME) and added to an amylose resin (New England Biolabs), incubating overnight at 4°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... 4 uL BbsI-HF (NEB R3539L, 1 uL T4 ligase (NEB M0202M), and 1uL of an XTEN linker-encoding DNA amplicon ...
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bioRxiv - Synthetic Biology 2022Quote: ... 4 uL BbsI-HF (NEB R3539L, 1 uL T4 ligase (NEB M0202M), and 1uL of an XTEN linker-encoding DNA amplicon ...
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bioRxiv - Genetics 2023Quote: ... 4 µl Luna universal qPCR Master Mix (New England Biolabs, Hitchin, UK) and 1.8 µl ultrapure water ...