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Citations for New England Biolabs :
951 - 1000 of 3540 citations for SUN domain containing protein 2 SUN2 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... the allelic lacI-containing shuttle vector pMQ123 was first digested with BamHI (New England BioLabs) and the targeted small regulatory RNAs were amplified using Phusion polymerase (New England BioLabs ...
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bioRxiv - Microbiology 2022Quote: ... The beads were resuspended in 1X PBS containing NuPage LDS Sample Buffer (New England Biolabs) and dithiothreitol (DTT ...
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bioRxiv - Biophysics 2023Quote: ... and BG-oligonuculeotides were labeled with myosin II containing a C-terminal SNAP-tag (NEB) in anion-exchange elution buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... CNCCs were re-suspended in DEPC-PBS containing 0.04% non-acetylated BSA (New England Biolabs). Cell viability was determined to be >95% by Trypan Blue staining ...
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bioRxiv - Cancer Biology 2023Quote: ... 30ng of genomic DNA was used for each reaction containing 7.5 U Φ29 (#M0269L, BioLabs), 1x Φ29 DNA polymerase buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... agarose plugs containing DNA were washed three times with 1× NEBuffer 3.1 (New England BioLabs) with 5-minute incubations ...
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bioRxiv - Genomics 2023Quote: ... either NT of EMC2 guide containing inserts were T4 ligated (ensure it is NEB #M0202M) with an insert to vector ratio of 1:2 for a 16 hours at 16C ...
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bioRxiv - Systems Biology 2023Quote: ... PCR amplification was carried out in a reaction mixture containing PCR Master Mix (NEB, M0543S), 0.5 μM of each primer ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... between long oligos containing the gRNA target sequences using Q5 polymerase (New England Biolabs, #M0491S). The vector and the PCR products were then integrated using Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... the Sox2-promoter-containing pCR Zero plasmid was digested with Xbal + SalI-HF (R3138S, NEB) and ligated (T4 Ligase 5 U/µL ...
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bioRxiv - Microbiology 2024Quote: ... coli DH5α F’Iq cells and transformants were selected on LB agar plates containing carbenicillin (NEB). The DNA sequence of the plasmid inserts for each transformant was verified before and after transformation into STm by electroporation ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were incubated in a complete medium containing 10 μM SNAP-Cell Block (NEB) to block all available pre-existing H3.3-SNAP (quench) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 35 µl of CRISPR reaction in 1x CutsmartT were added containing 75 nM LbaCas12a (NEB), 95 nM gRNA and varying concentrations of 60 bp synthetic dsDNA target ...
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bioRxiv - Bioengineering 2024Quote: ... media containing 100 μg/mL ampicillin and 109 p.f.u./mL of M13K07 helper phage (NEB) was added for overnight phage amplification in 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 20,000 to 50,000 cells were lysed in a buffer containing digitonin (#16359, New England Biolabs) and Tn5 transposase enzyme (Tagment DNA Enzyme and Buffer Kit ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were incubated in RA and SAG medium containing 1µM SNAP-Cell Block (NEB S9106S) or 100nM SNAP-Cell TMR-Star ligand (NEB S9105S ...
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bioRxiv - Microbiology 2020Quote: ... The soluble proteins were mixed with amylose resin (New England Biolabs) according to the manufacturer’s protocols.
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bioRxiv - Molecular Biology 2020Quote: The protein was expressed in NiCo21 (DE3) cells (New England Biolabs) grown in Overnight Express Instant TB Medium (EMD Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with DTT and proteins were degraded using proteinase K (NEB). Total cellular RNA was purified using the RNeasy maxi kit (QIAGEN ...
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bioRxiv - Developmental Biology 2021Quote: ... Cas9 protein (New England Biolabs, #M0646M, EnGen Cas9 NLS 20 μM) and 300 pg of sgRNAs along with 5 ng of GFP cRNA were co-injected into the X ...
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bioRxiv - Developmental Biology 2021Quote: ... in equimolar ratios and EnGen®Spy Cas9 NLS protein (NEB) into 1-cell zebrafish embryos.
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bioRxiv - Immunology 2021Quote: ... Deglycosylation was performed on beads using Protein Deglycosylation Mix II (NEB) in denaturing conditions according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... associated with EnGen™ Spy Cas9 NLS protein (New England BioLabs), using the 4D-Nucleofector™ System (Lonza ...
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bioRxiv - Physiology 2020Quote: ... were co-injected with 0.5 μl Cas9 protein (NEB, M0386, 20μM), diluted 1:10 ...
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bioRxiv - Cell Biology 2021Quote: ... Molecular weights were identified using a protein standard (BioLabs, Cat# P7712S). Beta-actin was used as the loading control ...
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bioRxiv - Biochemistry 2020Quote: ... morsitans salivary proteins were treated with PNGase F (New England Biolabs), resolved by SDS-PAGE and transferred onto a PVDF membrane (GE Healthcare ...
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bioRxiv - Bioengineering 2021Quote: Recombinant RfxCas13d proteins were expressed in E.coli NiCo21(DE3) (NEB C2529). Cells were grown in 1L of lysogeny (Luria-Bertrani ...
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bioRxiv - Neuroscience 2021Quote: ... 1 µl of lambda protein phosphatase (New England BioLabs, Ipswich, MA). Each mixture was incubated at 30 °C for either 1 ...
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bioRxiv - Plant Biology 2020Quote: ... the purified proteins (0.125 mg) or RNase If (1.25 U; NEB) were incubated at 25°C for 60 minutes with a fluorescent-labeled RNA substrate (5’-fluorescein isothiocyanate (FITC)-CUUUUAG(A20) ...
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bioRxiv - Molecular Biology 2022Quote: ... and α1-3,4 fucosidase (New England Biolabs, 4 U/μg protein). All enzymatic reactions were performed as a 1-step reaction with 1x Glycobuffer 2 (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... Molecular weights were determined using protein standards (New England Biolabs P7719).
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bioRxiv - Molecular Biology 2021Quote: ... The protein was then digested with LysC endoproteinase (0.25 µg; NEB) for 4 hours at 37°C ...
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bioRxiv - Bioengineering 2020Quote: IVPS was performed using PURExpress In Vitro Protein Synthesis Kit (NEB) with supplements (16 U RNase inhibitor (NEB) ...
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bioRxiv - Pathology 2021Quote: ... Soluble protein extracts were purified using amylose resin (New England Biolabs) and/or Ni-NTA agarose beads (Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... Magnetic beads conjugated with IgG Protein A/G (New England Biolabs) were pre-blocked with 0.5 mg/mL of sonicated salmon sperm DNA (Thermo Scientific ...
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bioRxiv - Biophysics 2021Quote: ... 100μg/ml single stranded DNA binding protein (gp32) from T4 (NEB), 277μg/ml T4 DNA clamp ...
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bioRxiv - Biochemistry 2021Quote: ... the purified proteins were incubated with excess streptavidin (New England Biolabs) for 10 minutes before analysis via gel-shift using SDS-PAGE ...
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bioRxiv - Developmental Biology 2021Quote: ... Cas9 protein (2.12 ng) (New England BioLabs Inc. Ipswich, MA, USA,) and gRNA (133 pg ...
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bioRxiv - Developmental Biology 2021Quote: ... Purified sgRNA and Cas9-NLS protein (New England Biolabs® Inc.) were diluted to 300ng/ul.
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bioRxiv - Molecular Biology 2021Quote: ... Samples were then added to protein A magnetic beads (NEB, S1425S) and rotated at 4°C for 4 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were purified using amylose resin column (New England Biolabs). Fractions were eluted and checked by silver staining ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were induced in SHuffle Express strains (New England Biolabs) upon addition of 1mM IPTG in cultures containing kanamycin 50 μg/mL and incubated with agitation at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: Protein extract samples (5µl) were subjected to EndoHf (New England Biolabs) treatment according to manufactures instructions ...
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bioRxiv - Genomics 2022Quote: ... EnGen Spy Cas9 NLS protein (20 μM) was purchased from NEB. The injection mixture consisted of 600 ng/μl Cas9 protein ...
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bioRxiv - Genetics 2022Quote: ... 0.8pg of 3xNLS-SpCas9 protein and 1mU of I-SceI (NEB) was injected per embryo at early 1-cell stage ...
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bioRxiv - Plant Biology 2022Quote: ... and MBP-HIS-GFP proteins were purified with amylose resin (NEB) and SUMO-HIS-BIN2 protein was purified with Ni-NTA Agarose (Qiagen).
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bioRxiv - Neuroscience 2024Quote: ... sgRNAs were mixed with Cas9 protein (M0646M NEB, or B25641 invitrogen) and coinjected into one-cell stage embryos using a microinjector (Eppendorf) ...
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bioRxiv - Biochemistry 2023Quote: ... each protein construct was transformed in either BL21 DE3 pLysS (NEB) harbouring a truncated OGT version with an intact catalytic core (OGTSW ...
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bioRxiv - Cell Biology 2024Quote: ... were combined with protein kinase buffer (PK buffer, New England Biolabs: 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... Dephosphorylation reactions were performed with Lambda Protein Phosphatase (Lambda PP) (NEB). 1,000 units of Lambda PP were utilized per µmol of Ycf1 protein in a 500 µL reaction ...